王恬
新生动物营养发育生理、饲料源生物活性物质的应用研究及动物生长的营养调控。
个性化签名
- 姓名:王恬
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:-
-
学科领域:
畜牧科学、动物医学
- 研究兴趣:新生动物营养发育生理、饲料源生物活性物质的应用研究及动物生长的营养调控。
王恬,男,江苏常州人。博士、教授、博士生导师。现任南京农业大学动物科技学院院长,动物营养与饲料科学研究所负责人。南京农学院畜牧兽医系七七级本科生,南京农业大学畜牧系饲料科学硕士研究生,香港大学(The Hong Kong University)动物生理与营养学博士研究生。1994年香港大学动物学系研究助理、2000年加拿大McGill大学高级访问学者。1982年至今,先后任南京农学院畜牧兽医系、畜牧系与动物科学系助教、讲师、副教授、副系主任、南京农业大学实验牧场场长、中外合资南京三益饲料添加剂有限公司(南农大参股)总经理、农业部南京奶牛原种场场长、南京农业大学动物科技学院院长。社会兼职主要有:全国饲料评审委员会委员、全国饲料工业标准化技术委员会委员、中国畜牧兽医学会动物营养学分会常务理事、家畜生态学分会理事、中国饲料工业协会理事、江苏省畜牧兽医学会副理事长、江苏省饲料工业协会副会长、江苏省饲料营养研究会副理事长。
多年来一直从事动物营养及饲料科学方面的科学研究、本科生教学和研究生指导工作。获“江苏省优秀教育工作者”称号。主持项目先后获江苏省供销合作总社科技进步三等奖、江苏省高等教育教研成果二等奖、教育部提名国家自然科学奖二等奖。先后主持或参与了国家科技攻关、863、国家自然科学基金、教育部青年骨干教师基金、农业部重点科技攻关及江苏省科技攻关及国际合作项目等。在国内外学术刊物上发表有关畜牧科技论文100多篇,主编或参编出版科技著作及教材18本(部)。论著代表作有:《优良鹅生产技术》,农业出版社(1990);《饲料添加剂应用原理及技术》,江苏科学技术出版社(1994);《高效饲料配方及配制技术》,中国农业出版社(2001);《畜牧学通论》,高等教育出版社(面向21世纪课程教材)(2002);《饲料学》,中国农业出版社(面向21世纪课程教材)(2003)。主要研究方向:新生动物营养发育生理”、“饲料源生物活性物质的应用研究”及“动物生长的营养调控”。
-
主页访问
2257
-
关注数
0
-
成果阅读
572
-
成果数
10
【期刊论文】胰岛素和酶解配方乳对初生仔猪胃肠道生长发育影响的研究
王恬, 邹仕庚, 郑春田, 陆治年, 许若军
动物营养学报,2001,(1):19~24,-0001,():
-1年11月30日
为研究胰岛素和酶解配方乳是否促进初生仔猪胃肠道的生长发育,本试验比较了饲喂配方乳、配方乳补加胰岛素(60mIU/ml)或酶(胰蛋白酶和糜蛋白酶)解配方乳三天后仔猪胃肠的重量,肠道长度,肠粘膜DNA、RNA和蛋白质含量,血浆中皮质醇、胰岛素和胃泌素的水平。饲喂配方乳补加胰岛素或酶解配方乳的仔猪,与饲喂配方乳的仔猪相比,胃、小肠、结肠的重量,小肠和结肠的长度,小肠粘膜DNA、RNA含量和结肠粘膜DNA、RNA、蛋白质含量均无显著变化(P>0.05);血浆中皮质醇、胰岛素水平也无显著差异(P>0.05)。但饲喂配方乳补加胰岛素的仔猪小肠粘膜尤其是回肠后段粘膜的重量(P<0.05)和蛋白质含量(P<0.01)显著高于饲喂配方乳的仔猪;饲喂酶解配方乳仔猪血浆的胃泌素水平均显著(P<0.01)高于饲喂配方乳的仔猪,酶解配方乳中可能含有刺激初生仔猪胃泌素释放的物质。
仔猪, 胃肠道, 胰岛素, 酶解配方乳
-
35浏览
-
0点赞
-
0收藏
-
0分享
-
168下载
-
0评论
-
引用
【期刊论文】胰岛素和酶解配方奶粉对初生仔猪小肠生长发育的影响
王恬, 郑春田, 陆治年, 邹仁庚, 许若军, 张建生
畜牧兽医学报,1999年,(5):405~413,-0001,():
-1年11月30日
为研究胰岛素和酶(胰蛋白酶和糜蛋白酶)解配方奶粉是否促进初生仔猪的组织生长和功能成熟,本试验比较了饲喂配方奶粉与饲喂配方奶粉补加胰岛素(60mIU/ml)或酶解配方奶粉3d后仔猪小肠的重量和长度,组织形态学结构,粘膜DNA,RNA和蛋白质含量、及小肠内容物中乳糖酶、麦芽糖酶和厉碱性磷酸酶活性。饲喂配方奶粉加胰岛素或酶解配方奶粉的仔猪,与饲喂配方奶粉的仔猪相比,小肠的重量、长度、组织形态学结构及粘膜的DNA和RNA含量均无显著变化(P>0.05)。但饲喂配方奶粉补加胰岛素的仔猪小肠粘膜尤其是回肠后段粘膜的重量和蛋白质含量显著高于饲喂配方奶粉的仔猪(P<0.05或P<0.01)。与仅喂配方奶粉的仔猪相比,饲喂配方奶粉补加胰岛素的仔猪小肠内容物中乳糖酶、麦芽糖酶和碱性磷酸酶总活性显著升高;饲喂酶解配方奶粉的仔猪小肠内容物中乳糖酶和碱性磷酸酶总活性显著升高(P<0.01)。结果表明,口饲胰岛素刺激小肠粘膜尤其是回肠后段粘膜重量和蛋白质含量的增加及小肠内容物中乳糖酶、麦芽糖酶和碱性磷酸酶总活性的升高;酶解配方奶粉中含有能刺激初生仔猪小肠发育的活性物质。
胰岛素,, 酶解配方奶粉,, 猪,, 小肠,, 乳糖酶,, 麦芽糖酶,, 碱性磷酸酶
-
42浏览
-
0点赞
-
0收藏
-
0分享
-
217下载
-
0评论
-
引用
【期刊论文】胰岛素和IGF-I对离体犊牛小肠上皮细胞增殖和吸收功能的影响
王恬, 马玉敏, 许若军
细胞生物学杂志,2002年,(3):176~179,-0001,():
-1年11月30日
本试验选择离体犊牛小肠上皮细胞,以细胞增殖率和葡萄糖吸收率作为细胞生长发育与功能成熟的指标,研究了胰岛素与胰岛素样生长因子-I(IGF-I)对细胞生长发育的影响。结果表明,胰岛素浓度为10μg/ml时,明显促进小肠上皮细胞的增殖和对葡萄糖的吸收,浓度达到50μg/ml时则抑制细胞的增殖和吸收(P<0.01)。IGF-I浓度为100ng/ml时,对促进小肠上皮细胞增殖和吸收葡萄糖的作用最强(P<0.01),但100ng/ml、500ng/ml和1000ng/ml三种不同浓度的IGF-I对刺激细胞增殖和提高吸收功能无显著差异(P>0.05)。
胰岛素IGF-I, 小肠上皮细胞
-
147浏览
-
0点赞
-
0收藏
-
0分享
-
270下载
-
0评论
-
引用
王恬, 王恬、傅永明、吕俊龙、蒋厚生、陈才勇、於朝梅、李亚平
畜牧与兽医,2003,(6):4~7,-0001,():
-1年11月30日
50 头21日龄断奶的胜利白猪随机分为5组,Ⅰ组(对照组)为基础日粮组;Ⅱ、Ⅲ、Ⅳ组分别为基础日粮+1%、2%、3%小肽营养素;Ⅴ组为基础日粮+2%SDPP。试验期28天。与对照组相比,断奶日粮中添加3%小肽营养素后,仔猪断奶至7周龄平均日增重提高33.91%(P<0.05),料重比下降8.4%(P<0.05),与添加2%SDPP的效果类似。添加小肽营养素后,仔猪十二指肠、空肠、回肠的绒毛长度增加、隐窝深度减小,这种趋势随着小肽营养素添加量的增加而提高。小肽营养素能刺激仔猪断奶后十二指肠食糜乳糖酶、淀粉酶、脂肪酶和胰蛋白酶的活性,以添加3%小肽营养素和2%SDPP效果较好。此外,小肽营养素可促进断奶仔猪免疫系统发育,增加血液中IgG浓度(P<0.05),降低腹泻率(P<0.05)。结果表明,小肽营养素可促进断奶仔猪生产性能及小肠发育,其作用随添加量的增大而提高,添加量以3%最好,并可代替2%SDPP。
小肽营养素, 断奶仔猪, 生长发育, 小肠, 免疫系统
-
53浏览
-
0点赞
-
0收藏
-
0分享
-
209下载
-
0评论
-
引用
【期刊论文】牛初乳酶解物对离体犊牛小肠上皮细胞增殖和吸收功能的影响*
王恬, 马玉敏, 许若军
动物营养学报,2002 ,(2):9~13,-0001,():
-1年11月30日
本试验以离体犊牛小肠上皮细胞为试验对象,选择细胞增殖率和葡萄糖吸收率作为细胞生长发育与功能成熟的指标,研究了牛初乳的蛋白酶水解产物对细胞生长发育的影响。结果表明,初乳经胃蛋白酶水解处理90分钟后所产生的水解物对离体犊牛小肠上皮细胞有明显的促增殖作用(P<0.01),而初乳经胰蛋白酶处理则不表现促增殖活性。初乳酶解物对促进细胞吸收葡萄糖的作用随着酶解处理时间的延长逐渐增强,初乳经胰蛋白酶水解处理90分钟或经胃蛋白酶水解处理150分钟时的产物对细胞吸收葡萄糖的促进作用分别达到最强(P<0.01)。结果表明,初乳酶解物即小分子蛋白质(肽)具有刺激离体小肠上皮细胞增殖和功能发育的活性。
初乳, 酶解物, 小肠上皮细胞
-
50浏览
-
0点赞
-
0收藏
-
0分享
-
146下载
-
0评论
-
引用
-
41浏览
-
0点赞
-
0收藏
-
0分享
-
131下载
-
0评论
-
引用
【期刊论文】Gastrointestinal Absorption of Insulin-like Growth Factor-Ⅰ in neonatal Pigs
王恬, Xu Ruo Jun, Wang Tian
Journal of Pediatric Gastroenterology & Nutrition, 1996, No.23, p. 430-437.,-0001,():
-1年11月30日
To determine if milk-borne insulin-like growth factor I (IGFI) is absorbed in neonatal piglets, recombinant human IGF-I was iodinated and then administered via an oral-gastric tube to newborn and 3-day-old piglets together with fluorescent isothiocyanate dextran (FITC-D70s, a macromolecular marker for assessment of gut closure). Results showed that total radioactivity and trichloroacetic acid (TCA)-precipitable radioactivity rose significantly in the plasma of both newborn and 3-day-old piglets 1 hour after oral-gastric administration of 125I-IGF-I, although the magnitude of increase was greater in newborns than in 3-day-old piglets. In contrast, significant absorption of FITC-D70s was observed only in newborn piglets but not in 3-day-old piglets. Chromatographic analysis revealed that 125I-IGF-I represented up to 20% of total plasma radioactivity in the newborns and up to 10% in the 3-day-old piglets. The 125I-IGF-I found in the plasma was mostly bound to IGF-binding proteins. These results indicate that milkborne IGF-I can be absorbed in neonatal piglets and the absorption is independent of gut closure.
Milk,, Peptide,, Gut closure,, Postnatal development,, Pig.,
-
47浏览
-
0点赞
-
0收藏
-
0分享
-
78下载
-
0评论
-
引用
【期刊论文】Functional structure and growth of the pancreas in postnatal growing animals
王恬, R.J. Xu a, T. Wang b and S.H. Zhang c
Biology of the Pancrease in Growing Animals, Elsevier, 1999, p.15-26.,-0001,():
-1年11月30日
The pancreas consists of two histologically distinct and functionally independent components, i.e., endocrine islets and exocrine acini. Cells in the endocrine islets produce hormones and regulate body metabolism via the blood circulation, while cells in the exocrine acini secrete digestive enzymes through the duct system into the duodenal lumen. The structure of the pancreas and its postnatal developmental changes in humans and laboratory rodents have been intensively reviewed in more recent publications (1-3). A similar review is lacking for the domestic animal species. This chapter focuses on morphological structure of the pancreas in pigs and its growth and structural changes during the postnatal period. Wherever possible, information derived directly from studies of pigs is used, or otherwise species origin of the data is defined. Comparisons with other domestic animal species are made when information is available.
-
44浏览
-
0点赞
-
0收藏
-
0分享
-
174下载
-
0评论
-
引用
王恬, Tian WANG, Ruo-Jun XU, Yuan-Qiang ZHANG, Chun-Tian ZHENG and Jian-Sheng ZHANG
中国组织化学与细胞化学杂志(英文稿),1999,(2): 48-54, 8.,-0001,():
-1年11月30日
This study examined the effects of milk protein pre-hydrolyzation on the crypt cell proliferation and morphological alterations in the small intestine in neonatal piglets. The newborn unsuckled Erhualian piglets were bottle-fed for 3 days with cow milk formula or pre-hydrolyzed cow milk formula. When compared with newborn pigs, the height of the intestinal villi decreased by 30-45% in jejunal region inmilk-fed piglets, while no significant change was observed in the intestinal villus height in piglets fed pre-hydrolyzed milk. Microscopic examination showed an atrophy of the intestinal villi and accelerated proliferation of crypt cells in the milk-fed piglets, possibly due to a hypersensitive reaction of the intestine to dietary protein. The results indicate that the nature of dietary protein had a significant effect on the intestinal structure. This finding may have a significant implication in infant care and animal feeding.
Hydrolyzed milk, Intestine, BrdU, Immunohistochemistry, Micrometry, Neonatal piglet.,
-
58浏览
-
0点赞
-
0收藏
-
0分享
-
81下载
-
0评论
-
引用
【期刊论文】Effects of Colostrum Feeding on Intestinal Development in Newborn Pigs
王恬, Wang Tian and Xu Ruo Jun
Biology of the Neonate, 1996, No.70, p.339-348.,-0001,():
-1年11月30日
To investigate what factors lead to the rapid postnatal tissue growth and functional maturation in the newborn intestine we compared intestinal tissue mass and digestive enzyme activities between newborn unsuckled piglets and piglets bottle-fed for 3 days with either intact porcine colostrum or trypsinized porcine colostrum. Bottle-feeding of colostrum or trypsinized colostrum led to a significant increase in weight and length of the small intestine and a significant increase in mucosal weight of the large intestine. The mucosal protein content in the small and the large intestine and the mucosal DNA content in the large intestine increased significantly following 3-days bottle-feeding of porcine colostrum or trypsinized colostrum. The total mucosal DNA contents in the small intestine of piglets fed colostrums or trypsinized colostrum were, respectively, 39 and 64% greater than that in the newborn unsuckled piglets. The numbers of cells labeled with BrdU in intestinal crypts also increased significantly in piglets fed both diets compared with that of newborns. But a marked increment in villus height was observed only in piglets fed intact colostrum. Brush-border membrane enzymes showed a differential response to the dietary treatment. Bottle-feeding of intact porcine colostrum, but not trypsinized porcine colostrum led to a significant increase in lactase, alkaline phosphatase and aminopeptidase specific activities in the small intestine. In contrast, the specific activity of maltase in the small intestine increased significantly with age irrespective of dietary treatment. These results indicated that genetic and dietary factors are involved in regulating postnatal intestinal development, and porcine colostrum contains a trypsin-labile component which can increase lactase and alkaline phosphatase activities in the newborn intestine.
Colostrum, Growth factor, Intestine, Enzyme.,
-
55浏览
-
0点赞
-
0收藏
-
0分享
-
118下载
-
0评论
-
引用