田华雨
博士 研究员 博士生导师
中科院长春应用化学研究所
生物医用高分子、纳米基因/药物载体、基因治疗、免疫治疗、肿瘤疫苗。
个性化签名
- 姓名:田华雨
- 目前身份:在职研究人员
- 担任导师情况:博士生导师
- 学位:博士
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学术头衔:
博士生导师
- 职称:高级-研究员
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学科领域:
有机高分子材料
- 研究兴趣:生物医用高分子、纳米基因/药物载体、基因治疗、免疫治疗、肿瘤疫苗。
田华雨,男,研究员,博士生导师。中国科学院长春应用化学研究所生态环境高分子材料重点实验室生物医用高分子课题组工作。2005年在中科院长春应用化学研究所取得高分子化学专业博士学位;曾在韩国科学技术院、日本九州大学做客座研究,在美国犹他大学做访问学者。2012年至今,在中科院长春应用化学研究所任研究员。
目前主要从事生物医用高分子、纳米基因/药物载体、基因治疗、免疫治疗、肿瘤疫苗等方面的研究。在JACS、Angew、AM、AFM、ACS Nano、Nano Lett等国际主流杂志发表SCI论文150余篇。获权中国发明专利25项。2007和2011年获得吉林省科技进步一等奖,2012年获得国家自然科学基金优秀青年基金资助。所开发的细胞用基因转染试剂实现产业化。
研究领域:生物医用高分子、纳米基因/药物载体、基因治疗、免疫治疗、肿瘤疫苗。
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成果阅读
854
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成果数
13
Advanced Materials,2019,31(40):1902885
2019年08月18日
Activatable theranostic agents that can be activated by tumor microenvironment possess higher specificity and sensitivity. Here, activatable nanozyme-mediated 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) loaded ABTS@MIL-100/poly(vinylpyrrolidine) (AMP) nanoreactors (NRs) are developed for imaging-guided combined tumor therapy. The as-constructed AMP NRs can be specifically activated by the tumor microenvironment through a nanozyme-mediated “two-step rocket-launching-like” process to turn on its photoacoustic imaging signal and photothermal therapy (PTT) function. In addition, simultaneously producing hydroxyl radicals in response to the high H2O2 level of the tumor microenvironment and disrupting intracellular glutathione (GSH) endows the AMP NRs with the ability of enhanced chemodynamic therapy (ECDT), thereby leading to more efficient therapeutic outcome in combination with tumor-triggered PTT. More importantly, the H2O2-activated and acid-enhanced properties enable the AMP NRs to be specific to tumors, leaving the normal tissues unharmed. These remarkable features of AMP NRs may open a new avenue to explore nanozyme-involved nanoreactors for intelligent, accurate, and noninvasive cancer theranostics.
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Biomaterials,2019,223():119470
2019年12月01日
Nowadays, two-dimensional (2D) nanomaterials with many fascinating physicochemical properties have drawn extensive attention as drug delivery platforms for cancer theranostics. Nevertheless, current existing 2D nanomaterial-based drug delivery systems normally undergo the bottlenecks of hash preparation process, low drug loading content and unsatisfactory therapeutic outcome. Herein, we developed a novel nanoparticles-induced assemble strategy to construct 2D nanosheets with ultra-high curcumin loading content of 59.6 % and excellent stability in water. Furthermore, a distinct photothermal effect and multimodal imaging property after polydopamine coating could be obtained, thereby leading to precise and efficient ablation of tumor in combination of curcumin-induced chemotherapy. More importantly, the design principle of our work offers novel facile strategy to assemble metal-binding drugs into 2D nanomedicine with high drug content and well-defined shapes.
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Biomaterials,2019,223():119459
2019年12月01日
Combination of photodynamic therapy (PDT) and photothermal therapy (PTT) generally requires different components to build a composite irradiated with different excitation lights. One component photoactive agent for enhanced combination of PDT and PTT under the excitation of a single wavelength light source is more urgent in tumor phototherapy via adjusting spatial arrangement of photoactive units. Herein, porphyrin-based covalent organic framework nanoparticles (COF-366 NPs) were synthesized to control the orderly spatial arrangement of the photoactive building units and firstly used for antitumor therapy in vivo. COF-366 NPs provide the simultaneous therapy of PDT and PTT under a single wavelength light source with the monitoring of photoacoustic (PA) imaging, which makes the operation simpler and more convenient. COF-366 NPs had achieved good phototherapy effect even in the face of large tumors. The prepared multifunctional COF-366 NPs open up a new avenue to phototherapeutic materials and expand the application range of covalent organic framework.
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Biomaterials,2019,216():119255
2019年09月01日
Targeted activation or enhancement is an attractive strategy in the design of nano-theranostics. However, the responsiveness of the nanoagents is restricted by the limited levels of intra-tumor stimuli. Herein, we constructed a positive feedback nanoamplifier by encapsulating glucose oxidase (GOx) in the ferric ions contained metal organic framework (MIL-100), and coating the nanoparticles with polydopamine modified hyaluronic acid (HA-PDA). The mechanism of action of the ensuing nanoamplifiers was three pronged: 1) the high intra-tumor acidity accelerated the release of GOx, which consumed endogenous glucose and “starved” the tumors, in addition to aggravating the local acidity and H2O2 levels; 2) the hydroxyl radicals (·OH) generated from the Fenton-like reaction between MIL-100 with H2O2 contributed to the chemodynamic tumor therapy and augmented the O2 microenvironment, which could be speeded up under acid condition; 3) the oxygen (O2) produced in the Fenton-like reaction relieved the intra-tumor hypoxia and ensured the enzymatic reaction of GOx, along with augmenting the photoacoustic signal of nanoamplifier. Preliminary experiments in tumor bearing mice showed that the nanoamplifier not only boosted the local acidity/H2O2/O2 levels in tumor site to successfully suppress the growth of tumors through the self-enhanced chemodynamic/starving therapy, but also achieved the photoacoustic imaging of tumors. Taken together, this novel nanoamplifier with the abilities of self-enhanced tumor imaging and therapy is a promising entrant in the field of anti-tumor theranostics.
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Chem. Mater.,2019,31(9):3313–3323
2019年04月16日
Photodynamic therapy (PDT) still faces a key challenge associated with its oxygen-dependent property, which limits its therapeutic efficiency against hypoxic tumor. To address the problem, covalent organic nanosheets (CONs) are prepared with a donor–acceptor molecular heterostructure. CONs can address the hypoxic-tumor PDT by two strategies, that is, type I PDT and type I PDT combined with photothermal therapy (PTT). On the one hand, the molecular heterostructure of CONs can afford highly efficient charge carrier separation, a long lifetime of electrons and holes can be obtained, the electrons can reduce O2 to form O2·–, and at the same time, the holes can oxidize water to produce ·OH. Therefore, type I PDT is obtained, which can be used to diminish the limits of hypoxia in type II PDT. On the other hand, the recombination of photoexcited species results in an important nonradiative attenuation, and thus the energy is emitted as heat. A combination of type I PDT and PTT serves as an effective way to get around the difficulties of hypoxia in PDT. Intravenous injection of CONs in nude mice followed by the combination of type I PDT and PTT under single-wavelength irradiation achieves significant tumor ablation.
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Biomaterials,2019,197():284-293
2019年03月01日
Despite multifunctional nanoparticles using for photothermal therapy can efficiently kill cancer cells, their further application is still hindered by the intrinsic high uptake in the reticuloendothelial system (RES) organs, causing the slow elimination from the body and potential toxicity to the body. Therefore, it is ideal to develop multifunctional nanoparticles which process the ability to effectively accumulate in tumors, while the nanoparticles can be rapidly excreted from the body via renal clearance after effective treatment. Herein, we report the multifunctional nanoparticles (FeTNPs) based on the coordination interaction of phenolic group and metal iron, which are composed of ferric iron, tannic acid (TA) and poly (glutamic acid)-graft-methoxypoly (ethylene glycol) (PLG-g-mPEG). FeTNPs exhibit the following highlighted features: (1) The effective accumulation in the tumor tissue is achieved based on EPR effect. (2) The dual photoacoustic (PA)/magnetic resonance (MR) imaging capacity can provide guidance for the photothermal therapy (PTT). (3) FeTNPs can be dynamically disassembled by deferoxamine mesylate (DFO) to accelerate elimination of the nanoparticles, thus reducing the potential toxicity for the body. The DFO triggered dynamic disassembling strategy may open a new avenue to overcome the dilemma between EPR effect and renal clearance.
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【期刊论文】Molecular Strings Significantly Improved the Gene Transfection Efficiency of Polycations
J. Am. Chem. Soc.,2018,140(38):11992–1200
2018年08月29日
High transfection efficiency and low cytotoxicity are the two key factors to be considered in the design of gene carriers. Herein, a novel and versatile gene carrier (PLL-RT) was prepared by introducing “molecular string” RT (i.e., p-toluylsulfonyl arginine) onto the polylysine backbone. The introduction of RT string contributed to the formation of multiple interactions between the polycationic gene carriers and cell membrane or DNA, as well as adopting α-helix conformation, all of which would be beneficial to enhance the gene transfection. In addition, RT string grafted onto other polycations such as hyperbranced PEI25k and dendrimer PAMAM could also acquire improved transfection efficiency and low cytotoxicity. Moreover, PLL-RT presented significant tumor inhibition effect in vivo. This work provided an effective strategy for constructing novel gene carriers with high transfection and low cytotoxicity.
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Angewandte Chemie International Edition,2018,57(32):10309-1031
2018年06月11日
Intrinsically integrating precise diagnosis, effective therapy, and self-anti-inflammatory action into a single nanoparticle is attractive for tumor treatment and future clinical application, but still remains a great challenge. In this study, bovine serum albumin–iridium oxide nanoparticles (BSA-IrO2 NPs) with extraordinary photothermal conversion efficiency, good photocatalytic activity, and a high X-ray absorption coefficient were prepared through one-step biomineralization. The nanoparticles allow tumor phototherapy and simultaneous photoacoustic/thermal imaging and computed tomography. More importantly, BSA-IrO2 NPs can also act as a catalase to protect normal cells against H2O2-induced reactive oxygen pressure and inflammation while significantly enhancing photoacoustic imaging through microbubble-based inertial cavitation. These remarkable features may open up the exploration iridium-based nanomaterials in theranostics.
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ACS Nano,2018,12(8):8255–8265
2018年08月08日
Low toxic theranostic nanoparticles that can simultaneously achieve effective tumor accumulation and rapid renal clearance are highly desired for imaging contrast agents and photothermal therapy (PTT) in tumor diagnosis and therapy. Herein, we report a one-pot method for preparing multifunctional nanoparticles (FeAP-NPs) based on the coordination interaction of natural polyphenols (anthocyanins) extracted from fruits, FeIII ions, and poly(l-glutamic acid)-g-methoxy poly(ethylene glycol) copolymers. The FeAP-NPs possess the following favorable advantages: (1) The components of FeAP-NPs originate from natural products, an endogenous element, and poly(amino acid) derivatives, guaranteeing their safety for in vivo application. (2) FeAP-NPs exhibit excellent dual photoacoustic (PA)/magnetic resonance (MR) imaging capacity and high photothermal efficiency. (3) FeAP-NPs can overcome the intractable dilemma of the enhanced permeability and retention (EPR) effect and renal clearance for nanomedicine through the dynamic disassembling ability, which induces a switch of the elimination pathway. Complete tumor ablation is realized by PTT in MCF-7-bearing nude mice under the precise guide of PA and MR imaging. The detailed evaluation of the safety, biodistribution, and elimination behaviors of FeAP-NPs is conducted in vitro or in vivo. This work provides a promising comprehensive solution for nanomedicine clinical application.
anthocyanins,, photoacoustic imaging,, magnetic resonance imaging,, photothermal therapy,, EPR effect,, dynamic disassembling
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Advanced Functional Materials,2018,28(22):1800490
2018年04月19日
Developing a comprehensive platform which has both diagnosis and therapeutic strategies is necessary for efficient tumor treatment. In this work, a Fuel Improved microRNA Explorer (FIRE) probe with signal amplification capability is designed for sensitive detection of microRNA-21 (miR-21), which is upregulated in most tumor cells. Besides, FIRE could be loaded by polyethylenimine (PEI)-modified gold nanorods (AuNR-PEI) via facile electrostatic interaction, which could avoid the complicated processes commonly used to covalently conjugate nucleic acid probes onto AuNRs. The as-fabricated AuNR-PEI/FIRE system could efficiently distinguish tumor cells from non-tumor cells. The fluorescence signals in MCF-7 breast carcinoma and HeLa cervical carcinoma cells treated with AuNR-PEI/FIRE are enhanced 7- and 4.5-fold, respectively, compared with non-amplification system. AuNR-PEI/FIRE improves tumor detection ability in vivo and exhibits excellent tumor inhibition efficacy under the fluorescence imaging and photoacoustic imaging guided photothermal therapy. This is the first time to utilize the combined application of amplified nucleic acid detection and photothermal effect derived from gold nanorods together with PA imaging in a facile manner to provide a promising theranostic strategy for accurate diagnosis and tumor therapy.
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