潘庆华
通过植物遗传学、植物病理学和分子生物学等手段来了解植物与病原菌,特别是水稻与稻瘟病菌之间存在的基因对基因相互作用的分子机制
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- 姓名:潘庆华
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学术头衔:
博士生导师
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学科领域:
农艺学
- 研究兴趣:通过植物遗传学、植物病理学和分子生物学等手段来了解植物与病原菌,特别是水稻与稻瘟病菌之间存在的基因对基因相互作用的分子机制
潘庆华 (1963年3月生),1983年7月华南农业大学植物遗传育种学专业毕业,获农学学士学位;其后,在中国农业科学院作物育种栽培研究所从事水稻抗病育种及其基础研究;1991年5月自费赴日本京都大学留学,并分别于1994年3月和1998年3月获农学硕士和农学博士学位;其后,在泰国科技开发署生物技术中心从事博士后研究;1999年10月31日作为国外引进人才返回华南农业大学工作,1999年12月起任该校植物病理学、植物遗传育种学和微生物学教授,博士生导师。主要研究兴趣在于:通过植物遗传学、植物病理学和分子生物学等手段来了解植物与病原菌,特别是水稻与稻瘟病菌之间存在的基因对基因相互作用的分子机制。主要研究方向及其成绩:(1)水稻抗病基因的定位、克隆及其功能研究;已经定位了11个水稻稻瘟病抗病基因,并克隆了其中4个基因。(2)稻瘟病菌无毒基因的定位、克隆及其功能研究;已经定位了16个稻瘟病菌无毒基因,并克隆了其中8个基因。(3)稻瘟病菌群体的分子遗传学研究;已经完成了近1000个稻瘟病菌株11个群体的遗传及致病型结构的动态或比较分析。独立主持了包括国家“863”计划、国家转基因植物产业化专项、国家自然科学基金在内的科研项目15项。在国内外重要杂志发表论文40多篇;参编著作4部;获得了农业部科学技术进步二等奖一项,农业部科学技术进步三等奖两项。多次应邀出席国际水稻生物技术大会、国际稻瘟病大会、国际植物与微生物分子相互作用大会、国际植物保护大会等并作大会报告或墙报交流。在实验室建设和管理以及学生培养方面也全面地与国际接轨,在建设世界一流的实验室以及培养具有国际感觉的学生方面作出了积极而有成效的探索,并因此而获得了国内外专家广泛的好评。中国植物病理学会生物技术委员会委员;《植物病理学报》和《华南农业大学学报》编委。
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【期刊论文】IdentiWcation and Wne mapping of AvrPi15, a novel avirulence gene of Magnaporthe grisea
潘庆华, Jun-Hong Mad, Ling Wang, Shu-Jie Feng, Fei Lin, Yi Xiao, Qing-Hua Pan
Theor Appl Genet(2006)113: 875-883,-0001,():
-1年11月30日
Avirulence of Magnaporthe grisea isolate CHL346 on rice cultivar GA25 was studied with 242 ascospore progenies derived from the cross CHL346
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潘庆华, J. W. Chen, L. Wang, X. F. Pang, Q. H. Pan
Mol Gen Genomics(2006)275: 321-329,-0001,():
-1年11月30日
Genetic analysis and fine mapping of a resistance gene against brown planthopper (BPH) biotype 2 in rice was performed using two F2 populations derived from two crosses between a resistant indica cultivar (cv.), AS20-1, and two susceptible japonica cvs., Aichi Asahi and Lijiangxintuanheigu. Insect resistance was evaluated using F1 plants and the two F2 populations. The results showed that a single recessive gene, tentatively designated as bph19(t), conditioned the resistance in AS20-1. A linkage analysis, mainly employing microsatellite markers, was carried out in the two F2 populations through bulked segregant analysis and recessive class analysis (RCA), in combination with bioinformatics analysis (BIA). The resistance gene locus bph19(t) was finely mapped to a region of about 1.0 cM on the short arm of chromosome 3, flanked by markers RM6308 and RM3134, where one known marker RM1022, and four new markers, b1, b2, b3 and b4, developed in the present study were co-segregating with the locus. To physically map this locus, the bph19(t)-linked markers were landed on bacterial artificial chromosome or P1 artificial chromosome clones of the reference cv., Nipponbare, released by the International Rice Genome Sequencing Project. Sequence information of these clones was used to construct a physical map of the bph19(t) locus, in silico, by BIA. The bph19(t) locus was physically de-fined to an interval of about 60 kb. The detailed genetic and physical maps of the bph19(t) locus will facilitate marker-assisted gene pyramiding and cloning.
Rice brown planthopper (, BPH), , Resistance gene, Genetic map, Physical map, Sequence information
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潘庆华, Shen Chen
,-0001,():
-1年11月30日
The famous rice cultivar (cv.), St. No.1, confers complete resistance to many isolates collected from the South China region. To effectively utilize the resistance, a linkage assay using microsatellite markers (SSR) was performed in the three F2 populations derived from crosses between the donor cv. St. No.1 and each of the three susceptible cvs. C101PKT, CO39 and AS20-1, which segregated into 3R:1S (resistant/susceptible) ratio, respectively. A total of 180 SSR markers selected from each chromosome equally were screened. The result showed that the two markers RM128 and RM486 located on chromosome 1 were linked to the resistance gene in the respective populations above. This result is not consistent with those previously reported, in which a well-known resistance gene Pif in the St. No.1 is located on chromosome 11. To confirm this result, additional four SSR markers, which located in the region lanked by RM128 and RM486, were tested. The results showed that markers RM543 and RM319 were closer to, and RM302 and RM212 completely co-segregated with the resistance locus detected in the present study. These results indicated that another resistance gene involved in the St. No.1, which is located on chromosome 1, and therefore tentatively designated as Pi37(t). To narrow down genomic region of the Pi37(t) locus, eight markers were newly developed in the target region through bioinformatics analysis (BIA) using the publicly available sequences. The linkage analysis with these markers showed that the Pi37(t) locus was mapped to a≈0.8 centimorgans (cM) interval flanked by RM543 and FPSM1, where a total of seven markers co-segregated with it. To physically map the locus, the Pi37(t)-linked markers were landed on the reference sequence of cv. Nipponbare through BIA. A contig map corresponding to the locus was constructed based on the reference sequence aligned by the Pi37(t)-linked markers. Consequently, the Pi37(t) locus was defined to 374 kb interval flanking markers RM543 and FPSM1, where only four candidate genes with the resistance gene conserved structure (NBS-LRR) were further identified to a DNA fragment of 60 kb in length by BIA.
Oryza sativa L., Magnaporthe grisea
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潘庆华, X.Q. Liu
,-0001,():
-1年11月30日
Blast resistance in the indica cultivar (cv.) Q61 was inherited as a single dominant gene in two F2 populations, F2-1 and F2-2, derived from crosses between the donor cv. and two susceptible japonica cvs. Aichi Asahi and Lijiangxintuanheigu (LTH), respectively. To rapidly determine the chromosomal location of the resistance (R) gene detected in Q61, random amplified polymorphic DNA (RAPD) analysis was performed in the F2-1 population using bulked-segregant analysis (BSA) in combination with recessive-class analysis (RCA). One of the three linked markers identified, BA1126550, was cloned and sequenced. The R gene locus was roughly mapped on rice chromosome 8 by comparison of the BA1126550 sequence with rice sequences in the databases (chromosome landing). To confirm this finding, seven known markers, including four sequence-tagged-site (STS) markers and three simple-sequence repeat (SSR) markers flanking BA1126550 on chromosome 8, were subjected to linkage analysis in the two F2 populations. The locus was mapped to a 5.8cM interval bounded by RM5647 and RM8018 on the short arm of chromosome 8. This novel R gene is therefore tentatively designated as Pi36(t). For fine mapping of the Pi36(t) locus, five additional markers including one STS marker and four candidate resistance gene (CRG) markers were developed in the target region, based on the genomic sequence of the corresponding region of the reference japonica cv. Nipponbare. The Pi36(t) locus was finally localized to an interval of about 0.6cM flanked by the markers RM5647 and CRG2, and co-segregated with the markers CRG3 and CRG4. To physically map this locus, the Pi36(t)-linked markers were mapped by electronic hybridization to bacterial artificial chromosome (BAC) or P1 artificial chromosome (PAC) clones of Nipponbare, and a contig map was constructed in silico through Pairwise BLAST analysis. The Pi36(t) locus was physically delimited to an interval of about 17.0 kb, based on the genomic sequence of Nipponbare.
Oryza sativa
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【期刊论文】稻瘟病菌群体的分子遗传学研究——广东省与江苏省稻瘟病菌群体遗传及致病型结构的比较分析
潘庆华, 吴伟怀, 王玲, 何艺郡
中国农业科学,2004,37(11):1628-1635,-0001,():
-1年11月30日
通过基于SRAP 标记的分子指纹分析法对由广东省40个菌株和江苏省42个菌株构成的试验群体进行了遗传结构分析。结果表明,在相似性系数取0.83 时,82个供试菌株被分为27 个宗谱;其中广东群体16 个宗谱,其宗谱频率为59.3%;江苏群体12 个宗谱,其宗谱频率为44.4%,由此说明前者比后者的遗传多样性丰富。值得指出的是,在全部27个宗谱中,只有1个宗谱是两个群体共有的,由此推测二者之间存在明显的遗传特异性。另一方面,利用中国、日本和IRRI 的三套鉴别品种分别对82个供试菌株进行了致病型结构分析。结果表明,在上述三套鉴别品种上,广东群体分别被划分为16、30 和20个小种(致病型),其小种频率分别为40.0%、75.0%和50.0%;而江苏群体则被分为8、23 和11个小种,其小种频率分别为19.0%、54.8%和26.2%。由此说明广东群体的致病型多样性也比江苏群体的丰富;在两个群体之间致病型多样性与遗传结构多样性存在相关关系。此外,对两个群体的致病型特异性和优势小种的构成进行了比较分析。结果显示,在两个群体之间存在高度的致病型特异性;优势小种的构成亦存在分明的差别。由此说明在两个群体之间致病型特异性与遗传结构特异性也是密切相关的。本研究的结果再一次说明,在进行有关病原菌群体的分子遗传学研究时,应该分别从遗传宗谱和致病型的多样性和特异性等4个方面来剖析其结构特征。
稻瘟病菌, 遗传多样性, 遗传特异性, 致病型多样性, 致病型特异性
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【期刊论文】稻瘟病菌群体的分子遗传学研究——广东省稻瘟病菌群体遗传及致病型结构的时空变化分析
潘庆华, 杨雪燕, 王玲, 何艺郡
中国农业科学,2004,37(10):1468-1473,-0001,():
-1年11月30日
为了全面、系统地了解最近几年来广东省稻瘟病菌群体遗传及致病型结构的时空变化特征,通过基于SRAP(sequence-related amplified polymorphism)标记的分子指纹方法对由广东省2000~2002年度各83个 菌株构成的试验群体进行了遗传结构分析。结果表明,当相似性系数为0.92 时,3个年度的供试菌株分别被分为33、25 和13个遗传宗谱,其宗谱频率分别为39.8%、30.1%和15.7%。由此推测,广东省稻瘟病菌群体在这3年间其遗传多样性呈减少趋势,而且2001~2002年度的减少幅度要比2000~2001年度的大。另一方,对上述3个群体进行了基于中国鉴别品种的致病型结构分析。结果表明,上述3个群体分别被划分为20、17和15个致病型,其致病型频率分别为24.4%、20.5% 和18.1%。由此说明,3个稻瘟病菌群体的致病型多样性大体上也呈减少趋势。本文对稻瘟病菌群体遗传结构与致病型结构的互动关系,以及稻瘟病菌群体致病性遗传结构与寄主群体抗性遗传结构的互动关系进行了分析。据此推测,广东省水稻品种抗性遗传结构的稳定化和简单化是造成其稻瘟病菌群体的遗传和致病型多样性减少的主要因素。
稻瘟病菌, 遗传结构, 致病型结构, 动态变化
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潘庆华, Q. H. Pan*†, L. Wang† and T. Tanisaka
Plant Pathology (1999) 48, 288-293,-0001,():
-1年11月30日
Blast, caused by Pyricularia grisea, is a major constraint on rice production. To widen genetic diversity for disease resistance, the Indian native rice cultivar Aus373 was screened by F2 segregation analyses to investigate the genetic basis of its high resistance. Aus373 was crossed with a series of Japanese differential cultivars (JDCs) and the Chinese susceptible cultivar Lijiangxintuanheigu (LTH). The resistance ratios of subsequent F2 progenies were used to determine the number of blast-resistance loci present as well as allelic relationships with known loci. Resistance of Aus373 was governed by dominant alleles at two loci, one at the Pi-k locus and the second apparently at a new locus linked to an isozyme gene Amp-1 with a recombination fraction of 37.9
allelism,, genetic linkage,, isozyme marker,, Japanese differential cultivar,, resistance genes,, rice blast
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潘庆华, Q. H. PAN, WANG, H. IKEHASHI, H. YAMAGATA and T. TANSAKA
Plant Breeding 117, 27-31(1998),-0001,():
-1年11月30日
The Chinese native rice cultivar 'Maowangu' expresses a high level of resistance to many races of rice blast (Pyricularia grisea) collected from North China and Japan. 'Maowangu' was crossed with 10 Japanese differential cultivars and the susceptible Chinese cultivar Lijiangxintuanheigu (LTH). Allclism tests were conducted in the F2 populations with rice blast races. The resistance of 'Maowangu' was governed by two dominant genes which were non-allelic to the resistance genes at seven loci: Pi-a, Pi-i, Pi-k, Pi-z, Pi-ta, Pi-b, and Pi-t. Toidentify the two resistance genes, two F3 lines of 'Shin 2/Maowangu' segregating 3R: 1S were selected for linkage tests in 1994. One was linked to marker genes C and Amp-3 on chromosome 6 with recombination frequencies of 35.8
Oryza satica-Pyricularia grisea-allelism test-avirulent race-Chinese native cultivar-linkage test-virulent race
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潘庆华, Menglan Zhu, Ling Wang, and Qinghua Pan
,-0001,():
-1年11月30日
The Chinese native cv. Q14 Expresses a high level of resistance to many isolates of Pyricularia grisea collected from Japan, Thailand, and China. Q14 was crossed to an indica-susceptible cultivar, Q61. To rapidly determine the Chromosomal location of the major resistance gene present in th cultivar, a linkage analysis using microsatellited markers was performed in the F2 population segregating 3R:1S (resistant/susceptible) through bulked-segregant analysis (BSA) in combination with recessiveclass analysis (RCA). Atotal of 189 microsatellite markers selected from each chromosome equally (with≈10 cemto,prgams) were tested with the BSA approach. Only two markers, RM151 and RM259, located on chromosome 1 showed positive and negative polymorphisms, respectively, for a resistance gene segregating in the population. To confirm the polymorphic markers, a total of 155 viable susceptible indiviuals were tested with the RCA approach. The markers RM151 and RM259 were found to link to the resistance gene with recomination frequencies of 11.9±2.8% and 9.7±8.0%, respectively. For further characterization of the resistance gene, 3 resistance genes mappedon chromosome 1, as well as 15 major resistance genes that might be employed in the breeing program, were selected for differential tests with 85 Chinese isolated. The resistancegene idetified in this research conveys reactions distinct form those conditioned by the 18 resistance genes. This new resistance gene tentatively was designted Pi27 (t).
Oryza sativa.,
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