赵凤林
新有机试剂的合成 ;药物分析测定(荷移光度法、荧光技术、非离子表面活性剂囊泡对药物的包封和缓释作用);生物大分子(蛋白质,核酸,糖类物质等)与小分子探针(试剂,染料,药物等)的超分子作用及其在生物大分子测定方面的应用研究。
个性化签名
- 姓名:赵凤林
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学术头衔:
博士生导师
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学科领域:
分析化学
- 研究兴趣:新有机试剂的合成 ;药物分析测定(荷移光度法、荧光技术、非离子表面活性剂囊泡对药物的包封和缓释作用);生物大分子(蛋白质,核酸,糖类物质等)与小分子探针(试剂,染料,药物等)的超分子作用及其在生物大分子测定方面的应用研究。
赵凤林,教授。1946年生,1970年毕业于北京大学化学系。1970年~1978年在校办制药厂有机车间工作,1979年至今在化学系分析教研室任教, 1993年7月至1994年7月作为访问学者在泰国朱拉隆功大学工作。
主要教学工作:分析化学理论课与实验课(本科生的主干基础课)
参编的教材和教学参考书:《分析化学》、《定量化学分析简明教程》、《基础分析化学实验》(北京大学出版社),《国际标准常规分析方法大全》(科学出版社)。
主要研究内容:
新有机试剂的合成 ;药物分析测定(荷移光度法、荧光技术、非离子表面活性剂囊泡对药物的包封和缓释作用);生物大分子(蛋白质,核酸,糖类物质等)与小分子探针(试剂,染料,药物等)的超分子作用及其在生物大分子测定方面的应用研究。
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116
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成果数
5
赵凤林, Ronghua Yang, † Ke'an Li, *, † Feng Liu, † Na Li, † Fenglin Zhao, † and Winghong Chan‡
Anal. Chem. 2003, 75, 3908-3914,-0001,():
-1年11月30日
A new optical chemical sensor for continuous monitoring of aliphatic aldehydes has been proposed based on the reversible chemical reaction between a new sensing reagent, 3,3',5,5'-tetramethyl-N-(9-anthrylmethyl)benzidine (TMAB), and the analytes. TMAB, containing two receptors and two fluorescent reporters, can perform dual fluorescence responses corresponding to the reactions of hydrogen ion and carbonyl compound. When immobilized in a plasticized poly(vinyl chloride) membrane, TMAB extracts aliphatic aldehydes from aqueous solution into the bulk membrane phase and reacts with the analyte by forming a Schiff base. Since the extraction equilibrium and chemical reaction are accompanied by fluorescence increase of the sensing membrane, the chemical recognition process could be directly translated into an optical signal. At pH 3.20, the sensor exhibits a dynamic detection range from 0.017 to 4.2mM n-butyraldehyde with a limit of detection of 0.003mM. The forward response time (t95) of the sensor is 3-5 min, and the reverse response time is 5-7 min. The responses of the sensor toward different kinds of aldehydes and ketones depend on the lipophilicity and the reactivity of the analytes. Since the fluorescence enhancement of the sensing membrane at 296nm/410nm is only related to the formation of Schiff base, the measurement of aldehydes is independent of pH.
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赵凤林, Ronghua Yang, † Ke'an Li, *, † Kemin Wang, ‡ Fenglin Zhao, † Na Li, † and Feng Liu†
Anal. Chem. 2003, 75, 612-621,-0001,():
-1年11月30日
In the present paper, a new cyclodextrin/porphyrin supramolecular sensitizer for zinc ion has been proposed based on the porphyrin dual fluorescence emission ratio. In aqueous solution, meso-tetraphenylporphyrin shows weak fluorescence, while in the presence of alkylated
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【期刊论文】A Ratiometric Fluorescent Sensor for AgI with High Selectivity and Sensitivity
赵凤林, Rong-Hua Yang, †, ‡ Wing-Hong Chan, *, † Albert W. M. Lee, † Ping-Fang Xia, † Hong-Kui Zhang, † and Ke'An Li*, ‡
J. AM. CHEM. SOC. 2003, 125, 2884-2885,-0001,():
-1年11月30日
The design and synthesis of fluorescent sensors with high selectivity and sensitivity for heavy and transition metal ions continues to grow at an unabated pace.1 Regardless of the signal transducing function, signaling changes of a fluorescent molecule at two bands that exhibit enhancement of one band at the expense of the other upon cation binding, a ratiometric measurement can be made which can increase the selectivity and sensitivity of a measurement and can eliminate most or all of the possible variability due to differences in instrumental efficiency and content of effective dye.2 Many investigations have been conducted to make ratiometric fluorescent sensors for metals;3 however, few reports have been explored for AgI.4 As many heavy cations are known as fluorescence quenchers, discrimination between AgI and chemically close ions presents a challenge.
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【期刊论文】Cyclodextrin-porphyrin supramolecular sensitizer for mercury(Ⅱ) ion
赵凤林, Ronghua Yang a, Ke'an Li a, *, Kemin Wang b, Feng Liu a, Na Lia, Fenglin Zhao a
Analytica Chimica Acta 469 (2002) 285-293,-0001,():
-1年11月30日
A new supramolecular sensitizer for mercury(Ⅱ) ion based on a cyclodextrin/porphyrin inclusion complex has been proposed. In aqueous base solution, meso-tetraphenylporphyrin (TPP) emits weak fluorescence. In the presence of alkylated-cyclodextrin, significant fluorescence enhancement is observed from TPP by forming a cyclodextrin/porphyrin inclusion complex. In addition, the formation of supramolecular complex causes a remarkable increase of the porphyrin metallation rate following the fluorescence quenching of TPP at its maximum emission wavelength. The quenching of TPP fluorescence at room temperature is fast and nearly complete upon Hg(Ⅱ) interaction. Based on these experimental observations, a simple, fast and low-cost fluorometric method for determination of Hg(Ⅱ) was developed. With the optimum conditions described, Hg (Ⅱ) in water from 5.0×10−8 to 2.0×10−5 mol l−1 can be determined with a limit of detection of 2.0×10−9 mol l−1. The method was applied in the analysis of water samples with satisfactory results.
Cyclodextrin, Porphyrin, Supramolecular complex, Mercury ion, Fluorescence
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赵凤林, Ying Zhang, RongHua Yang, *, Feng Liu, and Ke'An Li
Anal. Chem. 2004, 76, 7336-7345,-0001,():
-1年11月30日
A new zinc(Ⅱ) porphyrin conjugate with an appended pyrene subunit has been synthesized and shown to exhibit significant and analytical usefulness for fluorescence sensing toward imidazole derivatives. The molecular recognition was based on the bridging interaction of the imidazole ring of analyte with the zinc(Ⅱ) center of the porphyrin, while the transduction signal for the recognition process was the pyrene excimer fluorescence. The sensor was constructed and applied for fluorescence assay of histidine in aqueous solution by immobilizing the sensing material in a plasticized PVC membrane. When the membrane was bathed in an alkaline solution void of histidine, zinc(Ⅱ) porphyrin was present in the monomer form, and pyrene emitted monomer fluorescence at 378 and 397 nm. With the presence of histidine in the sample solution, histidine was extracted into the membrane phase and bridged with the Zn(Ⅱ) center of the porphyrin, causing the monomer porphyrin to be converted to its dimeric species. Since the formation of porphyrin dimmer was accompanied by the enhancement of pyrene excimer emission at 454 nm, the chemical recognition process could be directly translated into a fluorescent signal. With the optode membrane M1 described, histidine in sample solution from 6.76×10-7 to 5.01×10-3 M can be determined. The limit of detection was 1.34×10-7 M. The optical selectivity coefficient obtained for histidine over biologically relevant amino acids and anions met the selectivity requirements for the determination of histidine in biological samples. Serum histidine values obtained by the optode membrane fell in the normal range of the content reported in the literature and were in good agreement with those obtained by HPLC.
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