王正荣
生物技术和时间生物学
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- 姓名:王正荣
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学术头衔:
博士生导师
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学科领域:
医学微生物学
- 研究兴趣:生物技术和时间生物学
王正荣,男,47岁,教授,博士导师,四川省学术技术带头人,现任四川大学基础与法医学院副院长、四川省卫生厅副厅长、中国时间生物医学专业委员会副主任。主要从事生物技术和时间生物学研究工作。
先后主持完成国家自然科学基金、卫生部和教育部等基金课题以及国家“九五”攻关和“973”子课题10多项,获科研经费1000多万,先后发表论文120余篇,其中30余篇在国外刊物上发表,许多论文被国内外专家反复引用,美国、英国、法国、加拿大等西方发达国家的专家均来索取研究论文和要求合作。出版专著4本,出席国际性学术会议10多次,并在会上宣读论文,受到国内外同行专家们的高度评价。先后获卫生部、四川省科技进步奖三项,申请国家发明专利四项。1996年被四川省政府授予“四川省有突出贡献的中青年专家”的荣誉称号,获国务院专家特殊津贴。先后培养博士、硕士研究生30多人,博士后4名,开设并讲授研究生课程两门。
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成果数
4
王正荣, 屈艺, 黄翔, 杨春蕾, 刘柏林
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-1年11月30日
目的 探讨肝癌裸鼠移植瘤细胞DNA合成与端粒酶表达的近日节律。方法 在时辰同步化裸鼠中构建肝癌细胞SMMC27721裸鼠移植瘤,继续在程控光照条件下饲养,于光照后3 、9 、15 、21h取样,用流式细胞仪测定各时期细胞DNA含量,细胞周期分布及端粒酶活性水平。结果 肝癌裸鼠移植瘤细胞DNA 合成随近日节律变化,且伴随端粒酶活性的同步节律变化,呈近日节律特征。结论 肝癌裸鼠移植瘤的生长与端粒酶活性表达在静止相达高峰,为制定肿瘤时辰化疗方案提供了有价值的参考。
肝癌, DNA合成, 端粒酶, 近日节律, 基因表达
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王正荣, Li-Guo Chen, Zheng-Rong Wang*, Chao-Min Wan, Lee Chao, Julie Chao, Hao-Yang Xing
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-1年11月30日
Background The success of any gene-therapy approach depends on the survival of the genetically engineered cells that are implanted in the patient to deliver the therapeutic product. Immunoisolation of nonautologous cells within a microcapsule is a unique approach for gene therapy. Methods We employed an immunoisolation device that protects nonautologous cells from destruction, to implant human atrial natriuretic peptide (hANP)-producing Chinese hamster ovary (CHO) cells in two-kidney, one-clip (2K1C) hypertensive rats. CHO cells transfected with the plasmid CMV-cANP were encapsulated in biocompatible polycaprolactone (PCL) capsules, and then the PCL capsules were implanted into 2K1C hypertensive rats intraperitoneally. Results The implantation of encapsulated hANP-producing cells caused a significant delay of blood pressure (BP) increase 2 weeks post-implantation and the effect lasted for more than 5 months. The implantation of encapsulated hANP-producing cells also caused significant increases in renal blood flow (RBF), glomerular filtration rate (GFR), sodium output, urine excretion, and urinary cGMP levels. These beneficial effects were reflected morphologically by an attenuation of the glomerular sclerotic lesions, reduct on in cardiomyocyte size, tubular injury and renal arterial thickening. Immunoreactive hANP can be detected in the blood of 2K1C rats after implantation of the PCL capsules containing hANP-producing cells. Conclusions This study demonstrates the usefulness of encapsulated ANP gene transfected cells as a new tool for ANP gene delivery in studying renovascular hypertension and cardiovascular diseases. Thus, our results may have important implications for clinical use of transgene cells as therapeutic agents in the treatment of cardiovascular diseases.
atrial natriuretic peptide, encapsulated gene transfected cells, gene therapy, cell therapy, hypertension, polycaprolactone
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王正荣, LG Chen, ZR Wang, CM Wan, J Xiao, L Guo, HL Guo, G Corne lissen and F Halberg
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-1年11月30日
Renal excretion in experimental hypertensive rats implanted with encapsulated human atrial natriuretic peptide (hANP)-producing cells is circadian periodic. Chinese hamster ovary (CHO) cells transfected with the plasmid hANP-cDNA were encapsulated in biocompatible polycaprolactone capsules for intraperitoneal implantation into two-kidney, one-clip (2K1C) hypertensive rats. During a 12:12 light-dark cycle, as compared to control CHO cells, the implantation of encapsulated hANPproducing CHO cells was associated with an increase in the net excretion of water, sodium and potassium, and with a reversal of the advanced circadian phases related to renovascular hypertension in 2K1C rats. The increase in blood pressure postimplantation was delayed, and increases in renal blood flow, glomerular filtration rate, sodium output, urinary excretion and urinary cyclic GMP concentrations were also found. Implantation of encapsulated hANP-producing cells affects circadian rhythms in kidney excretion functions of 2K1C rats, and may be useful for the treatment of cardiovascular disease.
atrial natriuretic peptide, encapsulated transfected cells, hypertension, circadian rhythm
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【期刊论文】A New Peptide with Membrane-permeable Function Derived from Human Circadian Proteins
王正荣, Tao PENG, Ying-Hui LIU, Chun-Lei YANG, Chao-Min WAN, Yao-Qi WANG, and Zheng-Rong WANG*
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-1年11月30日
cultured neuroglial cells, we found that human Clock protein DNA-binding peptide [residue 35-47, hClock-(35-47)] had a translocation activity very similar to Tat-(48-60). The cellular uptake of hClock-(35-47) increases with the increase of incubation time and concentration. The internalization effect at 4℃ was same as that at 37℃. Internalization of hClock-(35-47) was saturable and could be inhibited by the excess of the other MPPs. Moreover, The uptake of these peptides were ignificantly inhibited in the presence of heparan sulfate. These results strongly suggested that the hClock-(35-47) shared a common or very similar internalization pathway with other MPPs. Furthermore, we injected rat through the common carotid artery with hClock-(35-47)-FITC peptide, and cryostat sections of the brain were prepared and observed using a fluorescence microscope. Result showed that the peptide had the ability to translocate through the blood-brain barrier. It is promising to provide a new safe carrier for the intracellular and encephalic treatment.
membrane-permeable peptide (, MPP), , cell membrane, hClock, DNA-binding peptide, blood-brain barrier
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