李清彪
个性化签名
- 姓名:李清彪
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
生物化学工程
- 研究兴趣:
李清彪,男,1963年出生,1990年于天津大学获得博士学位,1993年南开大学博士后出站后到厦门大学化学工程系任教,1995年2月至1996年9月在香港科技大学化工系从事访问学者和博士后研究,1996年9月任厦门大学化学工程系副系主任,1997年晋升为教授,1998年5月起任厦门大学化学工程系(现更名为化学工程与生物工程系)系主任至今。现任厦门大学教授、博士生导师。
已主持包括国家自然科学基金项目、教育部优秀青年教师资助计划项目、福建省科技计划重点项目在内的科研项目10多项,在国内外刊物上发表科研论文60多篇,参加的课题曾获福建省科技进步奖,同时获厦门市科技进步二等奖。发表教学论文近10篇。主讲的“化工工艺学”课程被评为福建省普通高等学校优秀课程。2001年被评为厦门市优秀教师,2004年被评为福建省优秀教师。
主要的社会兼职:中国化工学会理事;中国化工高等教育学会理事;中国化工教育协会理事;福建省化工学会副理事长;福建省石油学会常务理事;中国化学工程学报(英)编委;厦门市科技咨询评审专家;泉州市泉港区政府科技顾问。
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1363
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成果数
20
【期刊论文】高选择性基因工程菌E. coli SE5000生物富集水体中的镍离子
李清彪, 赵肖为, 卢英华, 何宁, 孙道华, 邓旭*
环境科学学报,2004,24(2):232~236,-0001,():
-1年11月30日
利用基因工程菌E.coli SE5000对水体中的镍离子进行富集研究。菌体细胞对Ni2+ 的富集速率很快,富集过程满足Langmuir等温线模型。经基因改造的基因工程菌不仅最大镍富集容量与原始宿主菌相比增加了4倍多,而且对pH值的变化呈现出更强的适应性。对离子强度及其它共存重金属离子的影响的实验结果表明:Na+,Ca2+,Cd2+,Pb2+ 的影响较小,但Mg2+,Hg2+,Cr3+ 和Cu2+ 所引起的负面效应较大。金属螯合剂EDTA 的存在对基因工程菌的富集行为影响很大。
大肠杆菌, 镍离子, 生物富集, 废水处理
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【期刊论文】Aerobic and anaerobic biodegradation of polyethylene glycols using sludge microbes
李清彪, Yi-Li Huang, Qing-Biao Li*, Xu Deng, Ying-Hua Lu, Xin-Kai Liao, Ming-Yuan Hong, Yan Wang
Process Biochemistry 40(2005)207-211,-0001,():
-1年11月30日
The aerobic and anaerobic biodegradation of polyethylene glycols (PEGs) in a model wastewater was investigated employing sludge microbes from Xiamen Terylene plant. The effect of molecular weight (MW) on the aerobic/anaerobic biodegradation of PEGs was assessed by performing shake flasks/sealed flasks experiments, respectively, using organic model wastewater containing PEG 600, 6000 and 20,000. In aerobic biodegradation, although there were differences between the degradation processes, the three kinds of PEG were all degraded by about 80% in 5 days, regardless of the wide diversity of MW. In anaerobic biodegradation, about 50% degradation of PEG 600 in 9 days, 40% degradation of PEG 6000 in 10 days and 80% degradation of PEG 20,000 in 6 days were obtained. The effect of nutrition on anaerobic degradation was also investigated. The biodegradation rate of PEG 6000 increased sequentially in the organic, inorganic and enriched organic media. The latter two were similar to each other. In the enriched organic media PEG 6000 was degraded by 50% in 10 days and 70% in 14 days, while in the organic media the degradation rate was 23% in 10 days and 50% in 14 days. It was notable that the anaerobic microbes could use PEG 6000 as sole carbon source, and PEGMWup to 20,000 was efficiently biodegraded in this study, either in aerobic or anaerobic way.
Biodegradation, Polyethylene glycol (, PEG), , Aerobic process, Anaerobic process
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【期刊论文】Bioaccumulation of nickel from aqueous solutions by genetically engineered Escherichia coli
李清彪, X. Deng a, *, Q.B. Li a, Y.H. Lu a, D.H. Sun a, Y.L. Huang a, X.R. Chen b
Water Research 37(2003)2505-2511,-0001,():
-1年11月30日
This study constructed a genetically engineered Escherichia coli JM109 which simultaneously expressednickel transport system andmetallothionein to remove andrecover Ni2+ from aqueous solution. Bioaccumulation process was rapidandfollowedlinearized Langmuir isotherm. A more than six-fold increase of Ni2+ binding capacity was obtainedby genetically engineered E. coli cells comparedwith original host E. coli cells. A pH assay showedgenetically engineered E. coli cells accumulatedNi 2+ effectively over a broadrange of pH (4-10). The presence of 1000mg/L Na+ andCa 2+, or 50mg/L Cd2+ or Pb2+ did not have a significant effect on Ni2+ bioaccumulation, while Mg2+, Hg2+ and Cu2+ poseda severe adverse influence on Ni2+ uptake by genetically engineered E. coli. Furthermore, genetically engineered E. coli cells did not require extra nutrients for Ni2+ bioaccumulation.
Escherichia coli, Nickel, Bioaccumulation, Genetic engineering
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【期刊论文】Biosorption and bioreduction of diamine silver complex by Corynebacterium
李清彪, Haoran Zhang, Qingbiao Li, ∗ Yinghua Lu, Daohua Sun, Xueping Lin, Xu Deng, Ning He and Shuzhen Zheng
J Chem Technol Biotechnol 80: 285-290 (2005),-0001,():
-1年11月30日
Corynebacterium strain SH09 separated from a silver mine was used for biosorption and bioreduction of diamine silver complex. The biosorption of the diamine silver complex was better than that of silver ions and the maximum of the former was about 350 (mg Ag) (g dried biomass)−1. After dried cells of SH09 were resuspended in the aqueous solution of diamine silver complex in the dark at 60℃ for more than 72h, transmission electron microscopy (TEM) observations showed that a large quantity of black particles whose diameter ranged from 10 to 15nm were formed on the cell wall. The particles were identified as being silver nanoparticles by X-ray diffraction (XRD) and UV-vis spectroscopy. Under the same conditions, no bioreduction of silver nitrate was found. According to IR spectra, some functional groups, such as the amide of the proteins, were involved in the processes of biosorption and bioreduction.
silver nanoparticles, bioreduction, biosorption, diamine silver complex, Corynebacterium
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67浏览
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334下载
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【期刊论文】Biosorption of lead by Phanerochaete chrysosporium in the form of pellets
李清彪, WU Juan, *, LI Qing-biao
Journal of Enitonmental Sciencer Vol.14, No.1, pp.108-114. 2002,-0001,():
-1年11月30日
The growth of Phanerochaete chryrosporium (ATCC 24725) in pellets was influenced by colture time, medium pH. CN, surlactant concentration, spote number in inoculum, and shaking rale. The reroval of Pb2+ from aqueous solution by this kind of mycellal pellets was studied. The results indicated that many factors affected biosorption. These factors included pH. Pb2+ concentration, co-ion, adsorption time, and chemical pretreatments of biomass. Under optimum biosorption conditions (pH 4.5, 27℃, 16H),the higheat lead uptake of 108mg/g, was observed with mycelial pellets of 1.5-1.7mm in diameter which were treated with 0.1mol/L NaOH solution before adsorption. Pretreatment of biomass with NaOH frther increased its biosorption capacity.
Phanerochaere churysosporium, mycelial pellets, lead ion, biosorption
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46浏览
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李清彪, LI Qing-Biao*, YOU Ying-Cai, TAO Xue and HE Bing-Lin**
CHINESE SCIENCE BULLETIN 1994, 39 (19): 1620~1623,-0001,():
-1年11月30日
phenylalanine ammonia-lyase,, deactivation,, L-phenylalanine,, chloride ion,, dissolved oxygen.,
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李清彪, X.W. Zhao a, M.H. Zhou a, Q.B. Li b, Y.H. Lu b, N. He b, D.H. Sun b, X. Deng b, *
Process Biochemistry 40(2005)1611-1616,-0001,():
-1年11月30日
Genetically engineered Escherichia coli JM109, which expressed merT-merP protein and metallothionein (MT), was employed in this study to evaluate its potential for mercury bioaccumulation accompanied by simultaneous cell propagation in Hg2+ solution containing organic matters. In contrast to original host, E. coli JM109 which could hardly grow at 1mg/L Hg2+ concentration, genetically engineered E. coli cells were able to propagate themselves in LB with Hg2+ concentration up to 7.4mg/L, although cell reproduction was delayed with increasing Hg2+ concentration. Simultaneously, this strain could accumulate Hg2+ from LB+ Hg2+ solutions effectively with more than 96% of mercury removal whether the cells were induced by IPTG or not. Cell propagation was somewhat inhibited by IPTG, but the capability of Hg2+-uptake of the induced cells was enhanced. These results suggested this strain could be used for the bioremediation of contaminated wastes containing mercury and organic matters.
Escherichia coli, Mercury, Cell propagation, Bioaccumulation
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李清彪, Qingbiao Li*, Songtao Wu, Gang Liu, Xinkai Liao, Xu Deng, Daohua Sun, Yuelin Hu, Yili Huang
Separation and Purification Technology 34(2004)135-142,-0001,():
-1年11月30日
that biosorption of Pb (II) ions was preferential to that of Cd (II) ions. In the single-ion situation, biosorption of Cd (II) ions and Pb (II) ions had the optimum adsorption conditions in common, which were the solution pH 4.5, temperature 27℃. The maximum uptake obtained at initial concentration of Cd (II) ions 50mg l−1, could reach 15.2mg g−1, for Pb (II) ions it could reach 12.34mg g−1. Both the adsorption equilibrium data fitted the Freundlich model well. Moreover, the uptake of Cd (II) ions had a less sensitive dependence on temperature than that of Pb (II) ions.
Binary ions adsorption, Heavy metals, Fine-chemical, Wastewater, Fungus
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64浏览
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198下载
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李清彪, LI Qingbiao**, LIAO Xinkai, WU Zhiwang, DENG Xu, UANG Yili, LU Yinghua, SUN Daohua, HONG Mingyuan and WANG Lin
Chinese d. Chem. Eng., 12(1)108-112 (2004),-0001,():
-1年11月30日
A new recycling hydrolytic-aerobic combined process was developed to treat the high concentration organic wastewater. Simulated wastewater containing 10g.L-1 starch with a CODCr value of 10000mg.L-Z was used. At first, the hydrolytic degradation and aerobic degradation process were examined in two batch reactors, respectively. In the stand-alone hydrolytic process, starch in the wastewater almost disappeared after 11h treatment, but CODcr remained as high as 5803mg.L-1 after two days. In the aerobic process, the biodegradation rate of starch was much slower during the first 11h than that in the hydrolytic process, although the ODcr removal efficiency reached 89.6e/0 and more than 90/o starch could be degraded after 37.5h. To determine the interaction effects of the two processes, a series of hydrolytic-aerobic combinations were examined in details. Hydrolytic process played an important role in the whole recycle combination process as it could improve the biodegradability of the high concentration starch wastewater. However, from the other experiments, the negative effect of hydrolytic acidification was found in the hydrolytic-aerobic combination, which suggested that the aerobic microorganisms needed time to adapt themselves to the acidic environment. The effect of the degrading time, which was spent in the hydrolytic and aerobic unit, and the number of circulations, with which the wastewater went through the two units were investigated. It was found that a recycle combination of 6h hydrolytic process with 12h aerobic process was highly effective and potentially economical, in which the final removal efficiency of CODCr and efficiency of starch degradation reached 94.1% and 98.8%, respectively.
activated sludge,, COD,, starch,, recycling,, hydrolytic-aerobic
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【期刊论文】Membrane of PVA coated on porous catalytic ceramic disks supported H3PW12O40
李清彪, Qing-Lin Liu*, Qing-Biao Li
Journal of Membrane Science 202(2002)89-95,-0001,():
-1年11月30日
Catalytic disks of porous ceramic plates supported H3PW12O40 were prepared. Cross-linked poly vinyl alcohol (PVA) composite membranes using the catalytic disks as the matrixes were obtained by spin coating. The dehydration reaction of butanediol (BD) to form tetrahydrofuran (THF) was taken as a model reaction to testify the catalytic activity of the disk, and which was found to be a good catalyst compared with the original H3PW12O40. The separative properties of the membrane were also examined by pervaporation using the same reacting mixture, and the water content in the reactor was much less than THF during the reaction coupled pervaporation.
Pervaporation, Composite membranes, Catalytic membranes, Liquid permeation, Membranes formation
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