王华芳
植物耐旱生物学,耐旱生物技术(生物工程)包括林木耐旱基因工程、细胞工程(组织培养、细胞培养)等;耐旱生理与分子生物学包括林木耐旱基因克隆、耐旱转基因植物分子和生理检测筛查;矮化耐旱节水绿化原理与技术等
个性化签名
- 姓名:王华芳
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
生物化学
- 研究兴趣:植物耐旱生物学,耐旱生物技术(生物工程)包括林木耐旱基因工程、细胞工程(组织培养、细胞培养)等;耐旱生理与分子生物学包括林木耐旱基因克隆、耐旱转基因植物分子和生理检测筛查;矮化耐旱节水绿化原理与技术等
北京林业大学教授,生物化学与分子生物学学科硕士和博士研究生导师。
为北京林业大学林学专业77级本科毕业生,1982年获农学学士学位,留校任教至今。在此期间,1987.7 获北京林业大学森林植物学专业硕士学位;1992.7 获中国农业大学植物生理生化专业理学博士学位;1995.03―04,芬兰Jämsäköski 林学院进修;1996赴香港浸会大学作植物耐旱信息传递访问学者; 1998赴南非Natal大学作植物生物技术博士后。
主讲研究生课程《植物细胞工程》、《植物组织培养》、《生物标记》等;本科生课程《生物工程》、《植物化学调控》等;培养植物生物技术方向研究生,一批硕士、博士生已经毕业;在读硕士研究生11名,博士生研究生7名。
主要研究方向为植物耐旱生物学,耐旱生物技术(生物工程)包括林木耐旱基因工程、细胞工程(组织培养、细胞培养)等;耐旱生理与分子生物学包括林木耐旱基因克隆、耐旱转基因植物分子和生理检测筛查;矮化耐旱节水绿化原理与技术等。
主持林木耐旱生物技术类研究课题,如国家863课题“刺槐、杨树耐旱新品种培育”,国家自然科学基金课题“胡杨根系向水性耐旱基因EST克隆与转化体系”,国家研究与开发课题“抗旱、耐盐转基因花卉(灌木)新品种培育”等。
研究成果在有关学术期刊上发表论文40多篇。申请发明专利5项;获科学技术鉴定成果2项,部级科技进步二等奖1项。
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【期刊论文】小麦品种农大98生殖生长期32P在器官间的再分配
王华芳, 高荣孚, 孙振元, 韩碧文, 刘淑兰
作物学报,1997,6(23):735~739,-0001,():
-1年11月30日
用同位素示踪法研究了小麦品种农大98在籽粒发育期中32P在各器官间的再分配。从开花期~完熟期,穗的32P以指数式从占全株总32P的7%增至46%;根的32P从47%减至7%;茎的32P以S形曲线从8%增至40%;叶以相反方式从38%减至7%。在小麦蜡熟期1天之内,6~12时,穗和茎分别输出32P22%。6、9、12、15、18和21时小麦体内出现32P分配高峰的器官依次为穗(50%)→茎(45%)→叶(41%)→根(30%)→茎(47%)→穗(66%)。由以上得出结论,32P在小麦体内的再分配是其分配重心在各器间的转移。
小麦, 32P, 再分配
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王华芳
林业科学,1996,3(32):219~224,-0001,():
-1年11月30日
用32P示踪原子法研究了一年生侧柏根系吸收磷的规律和影响因素。结果表明,生长和适应有代表性的3个种源侧柏,山东泰安(ST)、贵州黎平(GL)、和内蒙包头(NB)吸收32P的规律相同但数昊不同,ST、GL和NB吸收32P的速率遵从方程V=VoExp(Kt), 外流速率遵从E=Eo/(Km+t)2。种源侧柏的基因型差异、生理活性和环境条件都影响其对P的吸收。ST、GL和NB的半吸收期T1/2在营养液中分别是6.4、6.1和5.8h,在土壤分别是40.7\18.4和7.5h;在营养液中的外流最大量一半所需的时间Km分别是0.9\53和14min,ST、GL根阔别褐色区吸收32P比NB多,黄褐区类似而根尖区相反。苗木吸收的P大部分积累在根部,其余的向地上部分运输,ST、GL和NB上运32P的速度分别是3.0、4.0和2.2cm/h,上运量GL>ST>NB。
种源侧柏,, 苗木,, 在矿质吸收,, 磷,, 32P示踪原子法
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王华芳, 曾端香), 尹伟伦), 赵孝庆), 王华芳)**
北京林业大学学报,2000,3(22):90~95,-0001,():
-1年11月30日
该文综述牡丹(Paeonia suf fruticosa Andr.)的繁殖技术:播种、嫁接、分株、压条、扦插和组织培养等。嫁接是目前繁殖观赏牡丹的主要技术(其次为分株),但由于其繁殖系数低,限制了牡丹苗木的大量生产。牡丹器官发生组织培养已经研究了15a,但从接种到小植株驯化等工艺还存在问题,不能用于规模化生产。体细胞胚胎发生将是继器官发生之后以细胞克隆植株的生物技术。
牡丹,, 繁殖,, 种子,, 嫁接,, 分株,, 组织培养
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王华芳, 韩秀慧, 尹伟伦
林业科学,2004,4(40):189~192,-0001,():
-1年11月30日
Quadratic regressive factorial experi ment was used to optimize conce ntrations of 6-BA and NAA added in MS midu for shoot multiplication of miniaturd rose (Rosa cv. Sonnen Kind). The most amount of shoots was achieved by combining 6-E 1.93mg·L-1, NAA 0.33mg·L-1 into MS midum. The 6-BA and NAA conce ntrations were in a range of 1.68~2.75mg·L-1and 0.07~0.54mg·L-1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for days, then 50 shoots occurred. The probability of the event was 95%
二次回归正交设计,, 微型月季,, 组织培养,, 6-BA,, NAA
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王华芳, 王华芳), 尹伟伦), 张建华), 梁建生)
林业科学,2000,1(36):2~8,-0001,():
-1年11月30日
在土壤急性和缓慢干旱处理过程中,用液体闪烁计数器的单光子监测器测定了侧柏(Platycladus orien-talis)和大叶相思(Acacia auriculiform is)根系超弱发光(UWL)强度。结果表明,UWL随土壤干旱持续时间延长、土壤含水量(SWC)和根系含水量(RWC)下降而减少,P<0.001。土壤缓慢干旱时,植株根系UWL衰减较急性干旱时缓慢,UWL与植株水势(ψ)在P<0.1水平上显著相关。给干旱土壤恢复正常浇水,SWC、ψ和UWL均有所恢复,但UWL的恢复滞后于SWC和ψ结论是根系UWL作为反应树种抗旱性的生理指标比水势(ψ)灵敏。
侧柏(, Platycladus orientalis), ,, 大叶相思(, Acacia auriculiform is), ,, 超弱发光,, 信息传递,, 土壤干旱
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【期刊论文】Root and xylenl ABA changes in rsponse to soil drying in two woody plants
王华芳, WANG Huafang, ZHANG Jianhua*, LIANG Jiansheng and YIN Weilun
Chinese Science Bulletin Vol. 44 No.24 December 1999,-0001,():
-1年11月30日
Two woody plants, Platycladus orientalise (tolerant to drought) and Acacia auriculi-formis (sensitive to drought), have been subjected to rapid and slow drying. ABA levels in their roots and xylem sap have been determined using radioimmunoassay (RIA, sensitivity is 0.4 pmol per assay vial) with a monoclonal antibody against (+)-ABA. ABA contents of P. Orientalise and A. auriculiformis growing in well watered soil are 0.3 and 2.5 nmol
Platyeladus orientalise,, Acaisa aurieuliformis,, root,, xylcm sap,, ABA,, soil drying.,
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【期刊论文】Responses of waody plant root and xylem sap ATP to soil drying
王华芳, WANG Huafang, ZHANG Jianhua, LIANG Jiansheng and YIN Weilun
Chinese Science Bulletin Vol. 44 No.13 July 1999,-0001,():
-1年11月30日
The ATP in roots and xylem sap of two woody plant species, Platycladus orientalis and Acacia auriculiformis, subjected to rapid and slow soil drying has been determined enploy-ing firefly luciferase ATP assay method (sensitivity is at 10-12 mol ATPL-1). The ATP levels in the two species were 1.6nmol·gdw in roots, and 5.6umol·m-3 and 8umol·m-3 in xylem sap, respectively. When plants of P. orientalis and A. auriculiformis were sub-jected to rapid sap, respectively. When plants of P.orientalis and A. auriculiformis were sub-jected to rapid soil drying, respectively, as soil water content (SWC) decreased from the nor-mallevel (0.25g·g-1DW) to 0.02 and 0.06g·g-1DW, separately, plant water potential (ψ) dropped to -4 and -3.2 MPa, differently, the ATP in roots decreased 99.7% and 42%, re-spectively. When the rapidly dried soil was watered for up to 6 d, SWC and ψ were found to re-cover to their normal levels, but ATP content in roots of P. orientalis and A. auriculiformis re-covered by 10% and 23%, respectively. When plants of P. orientalis and A. auriculiformis were subjected to slow soil drying, respectively, as SWC was found to decrease from the nor-mal level to 0.1 and 0.13g·g-1DW, separately, ψ dropped to -2.5 and -2 MPa, differentlly, the ATP content in roots decareased 98% and 38%, respectively. When the slowly dried soil was waatered for up to 8 d, ATP levels in roots of P. orientalis and A. auriculiformis recovered by 30% and 24%, respectively. However, ATP concentrations in sylem sap were not directly influenced by either SWC or ψ The plot dots of xylem sap ATP concentration versus root ATP content were found to be distributed diagonally. It is concluded that ATP in roots is more sensi-tive in response to SWC changes than that to ψ and root-sourced ATP is a source of that in xylem sap. When plants are subjected to slow soil drying, the ATP levels in both root and xylem sap are higher than that to rapid soil drying.
Platyelanus orientalis,, Acacia auriculiformis,, roots., Xylem sap., ATP., Soil., Drought.,
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【期刊论文】Germination characteristics of Paeonia ostii var. lishizhenii seeds
王华芳, H Wang+ and J van Staden*
,-0001,():
-1年11月30日
The viability of Paeonia ostii var. lishizhenii seed used in this investigation was around 85% when tested under optimum conditions of tissue culture. The seed coat, endosperm extract, and GA3 significantly influenced embryo emergence. The seed coat exhibited some mechanical resistance while the coat and endosperm extracts exhibited inhibition. Removal of the radical end of the testa, soaking in GA3 (100-300 mg l-1) and BA (0.1 mg l-1) promoted radicle growth at 15
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【期刊论文】Establishment of in vitro cultures of tree peonies
王华芳, H Wangl and J van Staden*
South African Journal of Botany 2001, 67: 358-361,-0001,():
-1年11月30日
Decontamination of elite dwarf cultivars of the tree peonie was increased by immersion of explants (leaves, buds, roots, seeds) in a mixture of 20% ethanol and 0.075% HgCI2 for 7min. Seeds were best decoated and the embryos then disinfested. The phytohormones 2,4-D, BA and GA3 induced callus on both the embryonic axis and cotyledons. To form callus on young leaves and petioles of the dwarf cultivars ‘Xue Li Zi Yu' and ‘Zi Xia Lin' 4.52μM 2.4-D and 4.44μM BA were required. Addition of 1.44μM GA3 to this medium was needed for cv. 'Cai Lan' to survive and ultimately form callus.
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