凌文华
心血管疾病的营养防治研究
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- 姓名:凌文华
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学术头衔:
博士生导师
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学科领域:
食品卫生学
- 研究兴趣:心血管疾病的营养防治研究
凌文华,主要学习和工作经历:1989-1997 芬兰Kuopio大学攻读博士学位,加拿大和美国博士后学习;1997-现在 中山大学 营养学系教授(博士生导师)研究方向:心血管疾病的营养防治研究。
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10
【期刊论文】Physical activity, diet and cardiovascular disease risks in Chinese women
凌文华, Jing Ma, Zhaomin Liu, and Wenhua Ling*
PHN 393-9: 27, 10/9/2002-mn-54889,-0001,():
-1年11月30日
Objective: To investigate the relationship between different types and levels of physical activity and cardiovascular disease risk factors, including oxidative stress, blood lipids and insulin resistance, in a healthy female population in China. Method: Healthy women
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凌文华, Ruifen Zhang, Jing Ma, Min Xia, Huilian Zhu, and WengHua Ling
Manuscript received 9 December 3003. Initial review completed 29 December 2003. Revision accepted 16 January 2004.,-0001,():
-1年11月30日
High homocysteine levels in vitro promote the expression of inflammatory agents responsible for atherogenesis. We investigated the long-term effects of elevated plasma homocysteine on the expression of inflammatory molecules and attempted to elucidate their mechanisms. Male Sprague-Dawley rats (n=36) were randomly divided into 3 groups, which received the control AIN-93G diet, the control diet plus 10g/kg of L-methionine, or that diet without folate (0m/kg) for 14wk. Mild hyperhomocysteinemia was then induced in both experimental groups. The mildly hyperhomocysteinemic rats had markedly increased expression of intracellular adhesion molecule-1 (ICAM-1) in the aorta and elevated serum levels of monocyte chemoattractant protein-1 (MCP-1), compared to the control rats. The activation of nuclear factor B and formation of nitrotyrosine in the aorta were greater in rats with mild hyperhomocysteinemia than in control rats. Serum levels of malonyldialdehyde (MDA) were higher in mildly hyperhomocysteinemic rats than in control rats. These results suggest that the oxidative stress resulting from elevated plasma homocysteine stimulates the activation of nuclear factor B, and consequently increases the expression of the inflammatory factors in vivo. Such an effect may contribute to atherogenesis by enhancing the inflammatory response of the vascular endothelium.
atherosclerosis•homocysteine•inflammatory factors•nuclear factor B•oxidative stress
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凌文华, CHUN HU, †, JERZY ZAWISTOWSKI, ‡, WENHUA LING, §, AND DAVID D. KITTS*
J. Agric. Food Chem. 2003, 51, 5271-5277,-0001,():
-1年11月30日
Anthocyanins, present in fruits and vegetables as natural colorants, have been well characterized to possess bioactive properties. Anthocyanin components extracted from black rice (Oryza sativa L.indica) separated by gel filtration and identified using LC-MS were cyanidin 3-glucoside and peonidin 3-glucoside. A standardized extract of black rice pigmented fraction (BRE) containing known proportions of cyanidin 3-glucoside and peonidin 3-glucoside exhibited marked antioxidant activities and free radical scavenging capacities in a battery of in vitro model systems. Significant (p<0.05) prevention of supercoiled DNA strand scission induced by reactive oxygen species (specifically, peroxyl radical and hydroxyl radicals) and suppression of the oxidative modification of human low-density lipoprotein was obtained with BRE. In addition, BRE reduced (p<0.05) the formation of nitric oxide by suppressing inducible nitric oxide synthase expression in murine macrophage RAW264.7 cells, without introducing cell toxicity. The results of this study show that black rice contains anthocyanin pigments with notable antioxidant and anti-inflammatory properties for potential use in nutraceutical or functional food formulations.
Black rice, anthocyanin, antioxidant, nitric oxide
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凌文华, Lingbao Ai, M.D., Krystal K. Stephenson, Wenhua Ling, Chunlai Zuo, Perkins Mukunyadzi, James Y. Suen, Ehab Hanna, Chun-Yang Fan, Ph.D.
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-1年11月30日
The p16 (CDKN2a/INK4a) gene is an important tumor-suppressor gene, involved in the p16/cyclindependent kinase/retinoblastoma gene pathway of cell cycle control. The p16 protein is considered to be a negative regulator of the pathway. The gene encodes an inhibitor of cyclin-dependent kinases 4 and 6, which regulate the phosphorylation of retinoblastoma gene and the G1 to S phase transition of the cell cycle. In the present study, p16 gene promoter hypermethylation patterns and p16 protein expression were analyzed in 100 consecutive untreated cases of primary head and neck squamous cell carcinoma by methylation-specific PCR and immunohistochemical staining. The p16 promoter hypermethylation and apparent loss of p16 protein expression were detected in 27% and 74% of head and neck squamous cell carcinoma, respectively. By-2 test, history of alcohol or tobacco use was significantly correlated with the loss of p16 protein expression (P<005 and.05, respectively). When patient follow-up data were correlated with various clinical and molecular parameters, tumor size and nodal and clinical stage were the strongest prognostic predictors for disease-free survival (tumor recurrence) and for cause-specific and overall survival in patients with head and neck squamous cell carcinoma. Neither p16 promoter hypermethylation nor apparent loss of p16 protein expression appears to be an independent prognostic factor, although loss of p16 protein may be used to predict overall patient survival in early-stage head and neck squamous cell carcinoma.
Gene inactivation,, Head and neck squamous cell carcinoma,, p16,, Promoter hypermethylation.,
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【期刊论文】Metabolic partitioning of endogenous fatty acid in adipocytes.
凌文华, Wang T, Zang Y, Ling W, Corkey BE, Guo W.
1: Obes Res. 2003 Jul; 11 (7): 880-7,-0001,():
-1年11月30日
To develop an accurate new method to measure the partitioning of adipocyte endogenous fatty acids among different metabolic pathways, a critical step toward understanding the regulatory mechanism by which fat disposition is modulated. RESEARCH METHODS AND PROCEDURES: Isolated primary rat adipocytes were pre-incubated with isotope-labeled fatty acids. This allows determination of the specific activity of labeled fatty acids in the endogenous lipid pool. After the removal of exogenous fatty acids, the disposition of endogenous fatty acids into the three major metabolic pathways, namely, oxidation, re-esterification, and release into the medium, was measured independently. This was compared with the total lipolytic release of endogenous fatty acids, as measured by glycerol release. Adipocytes from normal fed and fasted animals were used to determine the effects of physiological variations on the metabolic fate of endogenous fatty acids. RESULTS: In normal fed animals, 0.2% of endogenous fatty acids were oxidized, 50.1% were released, and 49.7% were re-esterified. Fasting doubled the partitioning of fatty acids toward oxidation (p<0.05) in association with increased lipolysis (1.4-fold increase) (p<0.05). This effect was completely abolished by the addition of insulin to the cells (61% reduction) (p<0.05). DISCUSSION: The endogenous fatty acids in adipocytes are actively oxidized. This process can be regulated by altered physiological conditions or by insulin. Over time, it is possible that a small shift of fatty acids toward oxidation could have a significant impact on body fuel economy. This hypothesis needs to be tested.
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凌文华, Min Xia, Wen Hua Ling, , Jing Ma, David D. Kitts*, and Jerzy, Zawistowski*†
Manuscript received 18 September 2002. Initial review completed 6 October 2002. Revision accepted 28 October 2002.,-0001,():
-1年11月30日
Apolipoprotein (apo)E-deficient mice were used to study the antiatherogenic effect of black rice pigment fraction (BRF) and the possible mechanisms by which it inhibits atherogenesis. The apoE-deficient mice (n=45) were randomly divided into three groups and received AIN-93G diet (positive group), AIN-93G with 5g of black rice pigment fraction/100g (BRF group) and AIN-93G with 5g of white rice outer layer fraction/100g (WRF group) for 16wk. C57BL/6J mice (n=15) received AIN-93G and were used as a control group. Blood samples were collected for measurement of lipid concentration, antioxidized LDL antibody and nitric oxide concentration. Livers were extracted for determination of cholesterol concentrations, and aortas were used to determine cholesterol concentrations and inducible nitric oxide synthase protein and mRNA expression. Hearts were used to assess atherosclerotic plaque formation. The apoE-deficient mice fed the black rice pigment fraction diet had 48% (P<0.01) less atherosclerotic lesion area compared with apoE-deficient mice fed only the AIN-93G diet and 46% (P<0.01) less lesion area compared with mice fed the white rice outer layer fraction diet. This observation corresponded with significantly (P<0.05) lower total serum cholesterol, lower liver and aorta cholesterol (P<0.01) and higher HDL cholesterol (P<0.05) concentrations and lower (P<0.05) antioxidized LDL antibody titer in apoE-deficient mice fed the black rice pigment fraction diet compared with positive and WRF groups. Notwithstanding this, mice fed the black rice pigment fraction diet also had lower CD4' T lymphocyte expression (P<0.05) and weaker inducible nitric oxide synthase expression (P<0.05) compared with mice fed the AIN-93G diet and the white rice outer layer fraction diet, respectively. We concluded that the inhibition of atherosclerotic lesions of the black rice pigment fraction is attributed to the improvement in cholesterol accumulation and reduction in oxidative stress and inflammation.
black rice pigment fraction
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凌文华, Wen Hua Ling, , Lin Llin Wang, and Jing Ma
Manuscript received 24 July 2001. Initial review completed 24 August 2001. Revision accepted 1 ctober 2001.,-0001,():
-1年11月30日
ese diets. The serum concentration of most fatty acids except 18:1 did not differ between the WRF and the BRF groups. We conclude that the inhibition of atherosclerotic plaque formation derived from the black rice outer layer fraction in rabbits might be mediated by antioxidative or anti-inflammatory effects. J. Nutr. 132: 20-26, 2002,
rice
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凌文华, Wen Hua Ling, Qi Xuan Cheng, Jing Ma and Tong Wang
Manuscript received 21 September 2000. Initial review completed 21 October 2000. Revision accepted 7 February 2001.,-0001,():
-1年11月30日
The influence of white, red and black rice consumption on atherosclerotic plaque formation induced by hypercholesterolemia was investigated in rabbits. Male rabbits (n 5 36) were divided into five groups. They were fed a normal laboratory purified diet (normal group, n 5 6), a high cholesterol (0.5g/100g) diet (HC group, n 5 6), a high cholesterol diet with 30g/100g white rice (WR group, n 5 8), 30g/100g red rice (RR group, n 5 8), or 30g/100g black rice (BR group, n 5 8) for 10wk. Blood samples were collected for lipid measurements and aorta were removed for assessment of atherosclerotic plaques at the end of the protocol. The oxidant and antioxidant status of blood, erythrocytes, liver and aorta was evaluated. The area of atherosclerotic plaque was 50% lower in rabbits fed the red or black rice diets than in those fed the white rice diet. Compared with the HC and WR groups, serum HDL cholesterol and apolipoprotein (apo) A-I concentration were greater (P, 0.05) in the RR and BR groups. Liver reactive oxygen species (ROS) and aortic malondialdehyde (MDA) were significantly lower, and the liver total antioxidative capacity (TAC) and erythrocyte superoxide dismutase (SOD) activity were significantly higher in the RR and BR groups compared with the HC and WR groups. Red or black rice consumption reduced or retarded the progression of atherosclerotic plaque development induced by dietary cholesterol. The enhanced serum HDL cholesterol and apo A-I concentrations, and the increased antioxidant and decreased oxidative status may be mechanisms of the antiatherogenic effect of red or black rice. J. Nutr. 131: 1421-1426, 2001.
rice•atherosclerosis•antioxidative status·rabbits
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凌文华, Marilee Lougheed‡, Corey Ming Lum‡, Wenhua Ling‡, Hiroshi Suzuki§, Tatsuhiko Kodama§, and Urs Steinbrecher‡¶
Vol. 272, No.20, Issue of May 16, pp. 12938-12944, 1997,-0001,():
-1年11月30日
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凌文华, Wenhua Ling, *, Marilee Lougheed, Hiroshi Suzuki, §, Alison Buchan, ‡, Tatsuhiko Kodama, and Urs P. Steinbrecher*
Volume 100, Number 2, July 1997, 244-252,-0001,():
-1年11月30日
pecific for oxidized LDL, but characterization of these alternate receptors for oxidized LDL and evaluation of their quantitative importance in uptake of oxidized LDL has been difficult because of overlapping ligand specificity with SR-AI/II. The purpose of this study was to determine the importance of SR-AI/II in the removal of modified LDL from the bloodstream in vivo. The clearance rate of oxidized LDL from plasma in normal mice was very rapid, and 90% of injected dose was removed from the blood within 5min. Clearance rates of oxidized LDL were equally high in SR-AI/II knockout mice, indicating that this receptor is not required for removal of oxidized LDL from plasma. Surprisingly, there was no difference in the clearance rate of acetyl LDL in wild-type and SR-AI/II knockout animals. The plasma clearance of radioiodinated acetyl LDL was almost fully blocked by a 50-fold excess of unlabeled acetyl LDL, but the latter only inhibited oxidized LDL clearance by z 5%. Both modified LDLs were cleared mostly by the liver, and there was no difference in the tissue distribution of modified LDL in control and knockout mice. Studies in isolated nonparenchymal liver cells showed that Kupffer cells accounted for most of the uptake of oxidized LDL. Extensively oxidized LDL and LDL modified by exposure to fatty acid peroxidation products were efficient competitors for the uptake of labeled oxidized LDL by SR-AI/II–deficient Kupffer cells, while acetyl LDL and malondialdehyde-modified LDL were relatively poor competitors.
scavenger receptor•oxidized low density lipoprotein•atherosclerosis•macrophages•Kupffer cells
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