周总光
普外临床
个性化签名
- 姓名:周总光
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:-
-
学科领域:
外科学
- 研究兴趣:普外临床
暂无
-
主页访问
2501
-
关注数
0
-
成果阅读
470
-
成果数
10
周总光, Yang-Chun Zheng, Yu-Ying Tang, Zong-Guang Zhou, Li Li, Tian-Cai Wang, Yi-Ling Deng, Dai-Yun Chen, Wei-Ping Liu
World J Gastroenterol 2004; 10 (22): 3369-3373,-0001,():
-1年11月30日
AIM: To investigate the number, size, and status of lymph nodes within the mesorectum and to explore the prognostic significance of lymph node micrometastases in patients with rectal cancer. METHODS: Thirty-one patients with rectal cancer undergone total mesorectal excision between October 2001 and October 2002 were included. Mesorectal nodes retrieved from the resected specimens were detected with a combination of haematoxylin and eosin (HE) staining and immunohistochemistry (IHC). The relations between lymph node metastases, micrometastases and postoperative recurrence were analyzed. RESULTS: A total of 548 lymph nodes were harvested, with 17.7
-
43浏览
-
0点赞
-
0收藏
-
0分享
-
130下载
-
0评论
-
引用
周总光, Zong-Guang Zhou, You-Qin Chen, Xu-Bao Liu, Wei-Ming Hu, Bo-Le Tian, Huai-Qing Chen
World J Gastroenterol 2004; 10 (21): 3185-3187,-0001,():
-1年11月30日
AIM: To investigate the fluid shear stress induced changes of [Ca2+]i in neutrophils in pancreatic microcirculation of experimental acute pancreatitis (AP). METHODS: Wistar rats (n=36) were randomized into three roups. A model of AP was established by subcutaneous injection of caerulein. Low-shear 30 viscometer was used to provide steady fluid shear stress on separated neutrophils. The mean fluorescent intensity tested by flow cytometry was used as the indication of [Ca2+]i quantity. RESULTS: Under steady shear, cytosolic [Ca2+]i showed biphasic changes. The shear rate changed from low to high, [Ca2+]i in different groups decreased slightly and then increased gradually to a high level (P<0.05). A close correlation was observed between the cytosolic [Ca2+]i level and the alteration of fluid shear stress in regional microcirculation of AP. CONCLUSION: The increase of [Ca2+]i is highly related to the activation of neutrophils, which contributes to neutrophil adhesion to endothelium in the early phase of AP. The effect of fluid shear stress on [Ca2+]i may play a crucial role in pancreatic microcirculatory failure of AP.
-
57浏览
-
0点赞
-
0收藏
-
0分享
-
97下载
-
0评论
-
引用
周总光, Zhao Wang, Zong-Guang Zhou, Cun Wang, Gao-Ping Zhao, You-Dai Chen, Hong-Kai Gao, Xue-Lian Zheng, Rong Wang, Dai-Yun Chen, Wei-Ping Liu
World J Gastroenterol 2004; 10 (20): 2949-2953,-0001,():
-1年11月30日
AIM: To assess the microscopic spread of low rectal cancer in mesorectum regions to provide pathological eviden ce for the necessity of total mesorectal excision (TME). METHODS: A total of 62 patients with low rectal cancer underwent low anterior resection and TME, surgical specimen s were sliced transversely on the serial embedded blocks at 2.5 mm interval, and stained with hematoxylin and eosin (HE). The mesorectum on whole-mount sections was divided into three regions: outer region of mesorectum (ORM), middle region of mesorectum (MRM) and inner region of mesorectum (IRM). Microscopic metastatic foci were investigated microscopically on the sections for the metastatic mesorectal regions, frequency, types, involvement of lymphatic vessels and correlation with the original rectal cancer. RESULTS: Microscopic spread of the tumor in mesorectum and ORM was observed in 38.7% (24/62) and 25.8% (16/62) of the patients, respectively. Circumferential resection margin (CRM) with involvement of microscopic metastatic foci occurred in 6.5% (4/62) of the patients, and distal mesorectum (DMR) involved was 6.5% (4/62) with the spread extent within 3 cm of low board of the main lesions. Most (20/24) of the patients with microscopic metastasis in mesorectum were in Dukes C stage. CONCLUSION: Results of the present study support that complete excision of the mesorectum without destruction of the ORM is essential for surgical management of low rectal cancer, an optimal DMR clearance resection margin should be no less than 4 cm, further pathologic assessment of the regions in extramesorectum in the pelvis is needed.
-
40浏览
-
0点赞
-
0收藏
-
0分享
-
181下载
-
0评论
-
引用
-
56浏览
-
0点赞
-
0收藏
-
0分享
-
160下载
-
0评论
-
引用
周总光, Zong-Guang Zhou, Zhao Wang, Yong-Yang Yu, Ye Shu, Zhong Cheng, Li Li, Wen-Zhang Lei, Tian-Cai Wang
World J Gastroenterol 2003; 9 (7): 1477-1481,-0001,():
-1年11月30日
AIM: To assess the feasibility and efficacy of laparoscopic total mesorectal excision (LTME) of low rectal cancer with preservation of anal sphincter. METHODS: From June 2001 to June 2003, 82 patients with low rectal cancer underwent laparoscopic total mesorectal excision with preservation of anal sphincter. The lowest edge of tumors was below peritoneal reflection and 1.5-7 cm from the dentate line (1.5-5 cm in 48 cases, 5-7 cm in 34 cases). RESULTS: LTME with anal sphincter preservation was performed on 82 randomized patients with low rectal cancer, and 100% sphincter preservation rate was achieved. There were 30 patients with laparoscopic low anterior resection (LLAR) at the level of the anastomosis below peritoneal reflection and 2 cm above from the dentate line; 27 patients with laparoscopic ultralow anterior resection (LULAR) at the level of anastomoses 2 cm below from the dentate line; and 25 patients with laparoscopic coloanal anastomoses (LCAA) at the level of the anastomoses at or below the dentate line. No defunctioning ileostomy was created in any case. The mean operating time was 120 minutes (ranged from 110-220 min), and the mean operative blood loss was 20 mL (ranged from 5-120 mL). Bowel function was restored and diet was resumed on day 1 or 2 after operation. The mean hospital stay was 8 days (ranged from 5-14). Postoperative analgesics were used in 45 patients. After surgery, 2 patients had urinary retention, one had anastomotic leakage, and another 2 patients had local recurrence one year later. No interoperative complication was observed. CONCLUSION: LTME with preservation of anal sphincter is a feasible, safe and minimally invasive technique with less postoperative pain and rapid recovery, and importantly, it has preserved the function of the sphincter.
-
65浏览
-
0点赞
-
0收藏
-
0分享
-
142下载
-
0评论
-
引用
【期刊论文】Laparoscopic Management of Severe Acute Pancreatitis
周总光, Zong-Guang Zhou, Yang-Chun Zheng, Ye Shu, Wei-Ming Hu, Bo-Le Tian, Quan-Sheng Li, and Zhao-Da Zhang
Pancreas • Volume 27, Number 3, October 2003,-0001,():
-1年11月30日
severe acute pancreatitis,, laparoscopic surgery,, pancreatic necrosectomy,, intracavitary debridement,, external drainage,, management
-
35浏览
-
0点赞
-
0收藏
-
0分享
-
64下载
-
0评论
-
引用
周总光, Hong-Kai Gao, Zong-Guang Zhou, You-Qin Chen, Fang-Hai Han and Cun Wang
Hepatobiliary & Pancreatic Diseases International, Vol 2, No 3, 463-466,-0001,():
-1年11月30日
OBJECTIVE: To study the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in peripheral circulation and pancreatic microeirculation in rats with acute edematous pancreatitis (AEP). METHODS: The model of AEP was established with 50 Wistar rats, and the changes of PECAM-1 expression on PMNs from the splenic vein and inferior vena cava were determined by flow cytometry. RESULTS: PECAM-1 expression on PMNs showed no significant difference between pancreatic microcireulation and peripheral circulation at AEP2h and AEP4h time points. From the AEP4h to the AEP8h time point, PECAM-1 expression in peripheral circulation was up-regulated, but PECAM-1 expression in pancreatic microcirculation was down-regulated. PECAM-1 expression had a significant difference between pancreatic mierocirculation and peripheral circulation at the AEPSh time point (P<0.05). CONCLUSION: PECAM-1 expression on PMNs is in a converse way between pancreatic microcirculation and peripheral circulation in AEP.
acute edematous pancreatitis, peripheral circulation, pancreatic microcirculation, polymorphonuclear leukocyte, platelet endothelial cell adhesion molecules-1
-
55浏览
-
0点赞
-
0收藏
-
0分享
-
59下载
-
0评论
-
引用
周总光, Jian-Kun Hu, Zong-Guang Zhou, Zhi-Xin Chen, Lan-Lan Wang, Yong-Yang Yu, Jin Liu, Bo Zhang, Li Li, Ye Shu, Jia-Ping Chen
World J Gastroenterol 2003; 9 (12): 2690-2694,-0001,():
-1年11月30日
AIM: The study of immune response of open versus laparoscopical total mesorectal excision with anal sphincter preservation in patients with rectal cancer has not been reported yet. The dissected retroperitoneal area that contacts directly with carbon dioxide is extensive in laparoscopic total mesorectal excision with anal sphincter preservation surgery. It is important to clarify whether the immune response of laparoscopic total mesorectal excision with anal sphincter preservation (LTME with ASP) in patients with rectal cancer is suppressed more severely than that of open surgery (OTME with ASP). This study was designed to compare the immune functions after laparoscopic and open total mesorectal excision with anal sphincter preservation for rectal cancer. METHODS: This study involved 45 patients undergoing laparoscopic (n=20) and open (n=25) total mesorectal excisions with anal sphincter preservation for rectal cancer. Serum interleukin-2 (IL-2), interleukin-6 (IL-6), tumor necrosis factor a (TNFa) were assayed preoperatively and on days 1 and 5 postoperatively. CD3+ and CD56+ T lymphocyte count, CD3-and CD56+ natural killer cell (NK) count and immunoglobulin (IgG/IgM/IgA) were assayed preoperatively and on day 5 postoperatively. The numbers of CD3+ and CD56+ T lymphocytes and CD3- and CD56+ NK cells were counted using flow cytometry. An enzyme-linked immunosorbent assay (ELISA) was used for IL-2, IL-6 and TNFa determination. And IgG, IgM, and IgA were assayed using immunonephelometry. RESULTS: The demographic data of the two groups had no difference. The preoperative levels of CD3+ and CD56+ T lymphocyte count, CD3- and CD56+ NK count, serum IgG, IgM, IgA, IL-2, IL-6 and TNFa also had no significant difference in the two groups (P>0.05). The CD3+ and CD56+ T lymphocyte counts had no obvious changes after surgery in laparoscopic (d=-0.79
-
45浏览
-
0点赞
-
0收藏
-
0分享
-
57下载
-
0评论
-
引用
【期刊论文】Pancreatic microcirculatory impairment in experimental acute pancreatitis in rats
周总光, Zong-Guang Zhou, You-Dai Chen, Wei Sun, Zhong Chen
World J Gastroenterol 2002; 8 (5): 933-936,-0001,():
-1年11月30日
AIM: To study the feature of pancreatic microcirculatory impairment, especially the initial changes, in caeruleininduced experimental acute pancreatitis (AP). METHODS: The pancreatic microcirculation of caeruleininduced AP model was studied by intravital fluorescence microscopy with FITC-labeled erythrocytes (FITC-RBC), scanning electron microscopy of vascular corrosion casts, and light microscopy of Chinese ink-injected/cleared tissues. RESULTS: Animals in caerulein-treated group showed hyperamylemia (2), pancreatic oedema, infiltration of inflammatory cells in pancreas. Constrictions of intralobular arteriolar sphincters, presence of vacuoles in all layers of sphincter, and gross irregularity in capillary network of acini were found in the AP specimens. The decrease of pancreatic capillary blood flow (0.34 0.10 nl.min-1 vs 0.91 0.06 nl. min-1 of control, P<0.001), reduction of functional capillary density(277 13 cm-1 vs 349 8 cm-1 of control, P<0.001), and irregular intermittent perfusion were observed in caerulein-induced groups. CONCLUSION: Impairment and constriction of pancreatic intralobular arteriolar sphincter are the initial microcirculatory lesions in the early phase of acute pancreatitis, and play a key role in the pancreatic ischaemia and pancreatic microvascular failure in acute pancreatitis. Zhou ZG, Chen YD, Sun W, Chen Z. Pancreatic microcirculatory impairment in experimental acute pancreatitis in rats. World J Gastroenterol 2002; 8(5):933-936 INTRODUCTION Etiopathology of acute pancreatitis (AP) is not fully understood[1-19]. Microcirculatory impairment has long been recognized as one of the etiological factors of acute pancreatitis[20]. Pancreatic microcirculatory disturbance may act as initiating factor or aggravating/continuing factor. However, the mechanism of microcirculatory impairment in acute pancreatitis is complex; there are questions concerning local pancreatic microcirculatory change in acute pancreatitis and the features of pancreatic microcirculatory disturbance in various stages of AP remain subject to further study[21-28]. To investigate the feature of the pancreatic microcirculatory impairment in the early-stage of caerulein-induced experimental acute pancreatitis, dynamic method of microcirculatory research combined with static method had been carried out in this study.
-
34浏览
-
0点赞
-
0收藏
-
0分享
-
132下载
-
0评论
-
引用
周总光, Jian-Kun Hu, Zhi-Xin Chen, Zong-Guang Zhou, Bo Zhang, Jing Tian, Jia-Ping Chen, Li Wang, Chao-Hua Wang, Hong-Yan Chen, You-Ping Li
World J Gastroenterol 2002; 8 (6): 1023-1028,-0001,():
-1年11月30日
AIM: To assess the safety and efficacy of different intravenous chemotherapeutic regimens in patients with gastric carcinomas who had undergone gastrectomy. METHODS: A meta-analysis of all the relevant randomized controlled trials (RCTs) was performed. Language was restricted to Chinese and English. RCTs were identified from Medline and Embase (1980-2001/4), and Chinese Biomedicine Database (1990-2001/1). Literature references were checked at the same time. We included randomized and quasi-randomized trials comparing the efficacy of intravenous chemotherapy after gastrectomy with that of surgery alone in patients with confirmed gastric carcinomas who had undergone gastrectomy. Selection criteria were: randomized or quasi-randomized trials with following-up results; Trials could be double-blind, single-blind or not blind; Chemotherapy groups were given intravenous chemotherapy after gastrectomy without neo-adjuvant chemotherapy, intraperitoneal hyperthermic perfusion, radiotherapy or chemoimmunotherapy; Controlled group included those receiving gastrectomy alone. The following data were extracted: the number of survival and death by the end of the follow-up; the different agents and doses of the intravenous chemotherapy; the baseline of the chemotherapy group and the controlled arm; the serious adverse events; the statistical consideration; cost-effectiveness analysis. The statistical analysis was performed by RevMan4.1 software which was provided by the Cochrane Collaboration. A P value of<0.05 was considered statistically significant. Metaanalysis was done with random effects model. Heterogeneity was checked by chi-square test. Sensitivity analysis was performed by excluding the trials in which Jadad-scale was only 1 score. The result was expressed with odds ratio (OR) for the categorical variable. RESULTS: Fourteen trials involving 4543 patients were included. Meta-analysis was done with random effects model. Heterogeneity and sensitivity analysis were performed also. The effect of intravenous chemotherapy after gastrectomy was better than surgery alone (odds ratio 0.56, 95 %CI 0.40-0.79). There was a significant difference between the two groups by u-test (P=0.0008). Sensitivity analysis revealed the same difference (odds ratio 0.81, 95% CI 0.70-0.94). Of fourteen trials, only three studies were of high quality according to the Jadad-scale (with three score). There was one meta-analysis trial and the others, about ten trials, were of low quality. There was no trial which mentioned samplesize calculation, allocation concealment, intention-to-treat analysis. Most of the trials didn't describe the blind-procedure. There were five trials which detailed the side-effects according to the toxicity grade by WHO standard. The sideeffects halting treatment were haematologic and biochemical toxicity, debilitating nausea and vomiting. There were two patients died of chemotherapy toxicity. CONCLUSION: Based on the review, intravenous chemotherapy after gastrectomy may have positive treatment effect on gastric cancer. However, the evidence is not strong because of the general low methodologic quality of the RCTs. Therefore, we can't make the conclusion that intravenous chemotherapy after gastrectomy may have better treatment effect on gastric cancer than that of surgery alone. Rigorously designed, randomised, double-blind, placebo-controlled trials are required.
-
40浏览
-
0点赞
-
0收藏
-
0分享
-
198下载
-
0评论
-
引用