张峻峰
生物材料,基因靶向和基因治疗,组织工程,核酸调控。
个性化签名
- 姓名:张峻峰
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
生物化学
- 研究兴趣:生物材料,基因靶向和基因治疗,组织工程,核酸调控。
南京大学生命科学院医药生物技术国家重点实验室教授, 高分子科学与工程系兼职教授,博士生导师。
1985-1989,南京大学化学系高分子专业 本科
1989-1992 南京大学化学系高分子专业 硕士
1993-1996 日本京都大学生物医学工程研究中心 中日联合培养博士
1997-2000 新加坡微电子研究所 新加坡国立大学 Research Fellow
2000-今 南京大学生命科学学院
主要研究领域:生物材料,基因靶向和基因治疗,组织工程,核酸调控。
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2504
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成果阅读
510
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成果数
11
【期刊论文】Adhesion Improvement of Polytetrafluoroethylene/Metal Interface by Graft Copolymerization
张峻峰, Junfeng Zhang, Cheng Qiang Cui, Thiam Ben Lim, En-Tang Kang, Koon Gee Neoh
Surf. Interface Anal. 28, 235-239 (1999),-0001,():
-1年11月30日
Copper–poly(tetrafluoroethylene) (PTFE) and gold–PTFE laminates were prepared by surface graft copolymerization of glycidyl methacrylate (GMA) on argon plasma-pretreated PTFE films at an elevated temperature with simultaneous lamination to copper foils or gold substrates. The plasma pretreatment introduces peroxides, which are thermally degraded into radicals to initiate the graft copolymerization of GMA on the PTFE surface. Before lamination, the gold surface was pretreated with alkanethiols to form self-assembled monolayers and then subjected to an Ar plasma treatment. The modified surfaces and interfaces were characterized by x-ray photoelectron spectroscopy and the adhesion strength was assessed by the T-peel test method. By this technique, the Cu–PTFE or Au–PTFE laminates exhibit significantly improved adhesion strengths and the joints are delaminated by cohesive failure inside the bulk of the PTFE film.
poly(, tetrafluoroethylene), (, PTFE), , graft copolymerization, copper, gold, adhesion, x-ray photoelectron spectroscopy (, XPS),
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【期刊论文】Chemical Modification of Silicon (100) Surface via UV-Induced Graft Polymerization
张峻峰, Junfeng Zhang, Cheng Qiang Cui, Thiam Beng Lim, En-Tang Kang, Koon Gee Neoh, Sin Leng Lim, Kuang Lee Tan
Chem. Mater. 1999, 11, 1061-1068,-0001,():
-1年11月30日
Modification of argon plasma-pretreated single-crystal silicon wafer surface via UV-induced graft polymerization with various functional monomers, such as acrylamide (AAm), N,N- diamethylaminoethyl methacrylate (DMAEMA), and 2,2,2-trifluoroethyl acrylate (TFEA), was achieved. The modified Si(100) surfaces were characterized by X-ray photoelectron spectroscopy (XPS), imaging XPS, atomic force microscopy (AFM), and water contact angle measurements. The graft polymerization was affected by plasma pretreatment time and UV irradiation time. XPS results suggest that mild and brief plasma treatment is sufficient to cause surface oxidation and to generate sufficient peroxides and hydroxyl peroxides for the subsequent UV-induced graft polymerization in the presence of a vinyl monomer. Prolonged plasma treatment and the accompanying overoxidation of the silicon surface have an adverse effect on the graft polymerization. For all the cases investigated, the XPS results revealed that the grafted polymers form a thin layer with a thickness of 5 nm or less on the silicon surface. The AAm and TFEA graft-polymerized surfaces were uniform in morphology. However the DMAEMA graft-polymerized surface exhibited structural domains. Contact angle measurements further indicated that the silicon surface could be selectively made hydrophilic or hydrophobic through the proper choice of monomers used for graft polymerization.
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【期刊论文】Adhesion in water between ionic polymer surfaces
张峻峰, Junfeng Zhang , Jiangning Chen , Yoshito Ikada
Applied Surface Science 134 (1998) 116-124,-0001,():
-1年11月30日
To study the electrostatic interaction between two ionic polymer surface, a polyester film surface was graft-polymerized with a cationic monomer, N,N-dimethylaminoethyl methacrylate and an anionic monomer, acrylic acid. When the oppositely charged film pair was brought into contact in the presence of water, they showed strong adhesion instantaneously in water. The adhesion interaction was studied with both shear strength method and atomic force microscopy. The interaction depended on the graft density and the ionic nature of the graft chains. The addition of salt to the medium of the measurement caused a remarkable reduction in the adhesive interaction. These interactions were attributed to the coulombic force between the grafted ionic polymer chains.
Adhesion, Attractive interaction, Surface graft polymerization, Surface, Atomic force microscopy(, AFM),
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张峻峰, JUNFENG ZHANG, EMIKO UCHIDA, YOSHIKIMI UYAMA, YOSHITO IKADA
JOURNAL OF COLLOID AND INTERFACE SCIENCE 188, 431-438 (1997),-0001,():
-1年11月30日
To study the electrostatic interaction between two ionic polymer grafted surfaces in aqueous media, an atomic force microscopic tip surface was modified by graft polymerization of a cationic monomer, dimethylaminoethyl methacrylate (DMAEMA), after being coated with a thin layer of cyanoacrylate polymer. A poly(ethylene terephthalate) (PET) film which was the countersurface of the modified tip for the measurement of atomic force microscopy was also modified by surface graft polymerization of DMAEMA and an anionic monomer, acrylic acid (AAc) . An appreciable adhesive force was observed in water between the DMAEMA-grafted tip and the AAc grafted PET surface, while a repulsive interaction was noticed between the DMAEMA-grafted tip and the DMAEMA-grafted PET film. Addition of KCl to the medium for the atomic force measurement exhibited a remarkable reduction in the adhesive interaction. These interactions were attributed to the Coulombic force between the grafted ionic polymer chains.
surface graft polymerization, surface force, ionic interaction, atomic force microscopy (, AFM),
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【期刊论文】Adhesive Interaction in Water between Surfaces with Various Zeta Potentials
张峻峰, JUNFENG ZHANG, EMIKO UCHIDA, KYOJI SUZUKI, YOSHIKIMI UYAMA, YOSHITO IKADA
JOURNAL OF COLLOID AND INTERFACE SCIENCE 178, 371-373 (1996),-0001,():
-1年11月30日
To obtain polymer surfaces having different zeta potentials, a poly(ethylene terephthalate) (PET) film surface was graft-poly-merized with an anionic monomer, acrylic acid (AAc) , and a cationic monomer, N,N-dimethylaminoethyl methacrylate (DMA EMA) . In addition, a cellulose film was surface-modified with Chloroacetic acid to introduce anionic carboxyl groups and N,N-dimethylaminopropyl acrylamide (DMAPAA) to introduce cat-ionic dimethylamino groups. Through these surface modifications we could obtain films having zeta potentials ranging from /39 to -58 mV. Adhesive interaction in water between two surfaces of these films was measured with a tensile testing method after lapping the two surfaces in water. When one film was surface-grafted with cationic polymer chains, it exhibited significant adhesion instantaneously in water toward the other surface with a negative zeta potential, such as the surface-modified cellulose films, quartz, and the unmodified PET film. The quartz surface showed a detectable attractive force toward the cationic cellulose surface, but the force was weaker than that toward the cationically grafted surface. Apparently, no correlation was found between the zeta potential of the surfaces and their adhesive strength.
attractive interaction, adhesion, surface graft polymerization, zeta potential, polymer surface
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张峻峰, Ting Guo, , Jianning Zhao, Jianbin Chang, Zhi Ding, Hao Hong, Jiangning Chen, Junfeng Zhang
Biomaterials 27 (2006) 1095-1103,-0001,():
-1年11月30日
Cartilage defects as a result of disease or injury have a very limited ability to heal spontaneously. Recently, tissue engineering and local therapeutic gene delivery systems have been paid much attention in the cartilage natural healing process. Gene-activated matrix (GAM) blends these two strategies, serving as local bioreactor with therapeutic agents expression and also providing a structural template to fill the lesion defects for cell adhesion, proliferation and synthesis of extracellular matrix (ECM). In the current study, we used chitosangelatin complex as biomaterials to fabricate three-dimensional scaffolds and plasmid DNA were entrapped in the scaffolds encoding transforming growth factor-β1 (TGF-β1), which has been proposed as a promoter of cartilage regeneration for its effect on the synthesis of matrix molecules and cell proliferation. The plasmid DNA incorporated in the scaffolds showed a burst release in the first week and a sustained release for the other 2 weeks. The gene transfectd into chondrocytes expresses TGF-β1 protein stably in 3 weeks. The histological and immunohistochemical results confirmed that the primary chondrocytes cultured into the chitosan-gelatin scaffold maintained round and owned characters of high secretion of specific ECM. From this study, it can be concluded that this gene-activated chitosan-gelatins matrix has a potential in the application of cartilage defects regeneration.
Cartilage defects, Gene-activated matrix, Transforming growth factor-β1, Chitosan, Gelatin
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张峻峰, Shuying Gao, Jiangning Chen, Lei Dong, Zhi Ding, Yong-hua Yang, Junfeng Zhang,
European Journal of Pharmaceutis and Biopharmaceutis 60 (2005) 327-334,-0001,():
-1年11月30日
Delivery of oligonucleotide to specific cells and maintenance of its biological function are important for nucleic acid therapy. The objective of this paper is to demonstrate that galactosylated low molecular weight chitosan (gal-LMWC) is a safe and effective vector of antisense oligonucleotide (ASO) and plasmid DNA for the hepatocyte targeting delivery. Gal-LMWC has been successfully prepared and MTT cytotoxic assay shows that cytotoxicity of gal-LMWC is lower than that of high molecular weight chitosan (HMWC) and low molecular weight chitosan (LMWC) in HepG2 cells. Using a complex coacervation process, gal-LMWC can form stable nano-complexes with plasmid DNA or with ASO by the electrostatic interaction. The morphometrics, particle size, and the zeta potential of gal-LMWC/ASO complexes and gal-LMWC/plasmid DNA complexes are very similar. The transfection efficiency by using gal-LMWC vector is significantly higher than that of naked DNA or naked ASO in HepG2 cells. Transfection efficiency of gal-LMWC/ASO complexes and gal-LMWC/plasmid DNA complexes depends on the molar ratio of the positive chitosan amino group and the negative DNA phosphate group (N/P ratio) strongly. Inhibition experiments confirm that the enhanced transfection efficiency is due to the ASGR mediated endocytosis of the gal-LMWC/ASO complexes or gal-LMWC/DNA complexes. These results suggest that gal-LMWC can be used in gene therapy to improve the transfection efficiency in vitro and in vivo.
Galactosylated low molecular weight chitosan, Plasmid DNA, Antisense oligonucleotide, Hepatocyte targeting, Transfection efficiency, Non-viral vector
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张峻峰, Zhi Ding, Jiangning Chen, Shuying Gao, Jianbing Chang, Junfeng Zhang, , E.T. Kang
Biomaterials 25 (2004) 1059-1067,-0001,():
-1年11月30日
Surface functionalization of biodegradable poly-l-lactic acid(PLLA) was achievedby plasma coupling reaction of chitosan. The structure of modified PLLA surfaces was characterized by contact angle measurements and X-ray photoelectron spectroscopy. Two cell lines, L929 (mouse fibroblasts) andL02 (human hepatocytes), were cultured on the modified PLLA surface. It was found that cells cultured on this film could hardly spread and tend to become round, and the film was demonstrated to be a poorly adhering substrate. However, cells grown on this substrate can proliferate at almost the same speed as cultured on a glass surface. These results suggest that the new substrate can be used to control the morphology of cells, and has potential applications in tissue engineering. It may be helpful in understanding the mechanism of the switch between cell phases of growth and differentiation, which is necessary for the design of tissue regeneration biomaterials.
Chitosan, Poly-l-lactic acid, Cell culture, Plasma polymerization
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【期刊论文】Galactosylated low molecular weight chitosan as DNA carrier for hepatocyte-targeting
张峻峰, Shuying Gao, Jiangning Chen, Xuerong Xu, Zhi Ding, Yong-Hua Yang, Zichun Hua, Junfeng Zhang
International Journal of Pharmaceutics 255 (2003) 57-68,-0001,():
-1年11月30日
Chitosan has the potential for DNA complexation and is useful as a non-viral vector for gene delivery. Highly purified low molecular weight chitosan (LMWC) was prepared. Lactobionic acid (LA) bearing galactose group was coupled with LMWC for liver-specificity. A series of galactosylated-LMWC (gal-LMWC) samples covering a range of galactose group contents were prepared. The chitosan/DNA complexes were obtained using a complex coacervation process. Gal-LMWCs were used to transfer pSV-_-galactosidase reporter gene into human hepatocellular carcinoma cell (HepG2), L-02, SMMC-7721, and human cervix adenocarcinoma cell line (HeLa) cell lines in vitro. Transfection efficiency of gal-LMWCs was evaluated by _-galactosidase assay and compared with those of lipofectin, calcium phosphate (CaP), high molecular weigh chitosan (HMWC) and LMWC. Gal-LMWC/DNA complex shows a very efficient cell selective transfection to hepatocyte. The transfection efficiency of gal-LMWCs increased with the improvement of the galactosylation degree. Cytotoxicity of gal-LMWCwas determined by 3-(4,5-dimethylthiazd-2-yl)-2,5-diphenyltentrazolium bromide (MTT) assay and the results show that the modified chitosan has relatively low cytotoxicity, giving the evidence that the modified chitosan vector has the potential to be used as a safe gene-delivery system.
Chitosan, Non-viral gene delivery, Galactosylated chitosan, Liver-specificity
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【期刊论文】Cytotoxic effects of environmentally relevant chlorophenols on L929 cells and their mechanisms
张峻峰, J. Chen, J. Jiang, F. Zhang, H. Yu, J. Zhang
Cell Biology and Toxicology. 2004; 20: 183-196,-0001,():
-1年11月30日
The chlorophenol chemicals(CPs) are a major class of widely distributed and frequently occurring persistent environmental pollutants. Pentachlorophenol (PCP) has been proposed to be procarcinogen in rodents and in possibly human beings. Human beings also easily expose to other chlorophenol chemicals, including 4-chlorophenol (CP), 2,4-dichlorophenol (DCP),2,3,4-trichlorophenol(TCP), prompting this investigation of their comparative cytotoxic effects and cell death mechanisms, assayed in fibroblast L929 cells, The effective concentration for half-maximal response (EC50) values at 24 h for CP, DCP, TCP, and PCP are 2.18, 0.83,0.46, and 0.11 mmol/L respectively and the EC50 values at 48 h are 1.18, 0.13, 0.08, and 0.06 mmol/L respectively by using 3-(4,5-dimethylthiazd-2-yl)-2, 5-diphenyltentrazolium bromide (MTT) reduction assay. A clear structure-activity relationship was observed between toxicity of CPs and their octanol-water partition coefficients. The further studies indicate that CP, DCP, and TCP induce apoptosis in L929 cells in a concentration or time-dependent manner, but PCP mediates cell death more characteristic of necrosis than apoptosis. These results not only demonstrate that L929 cell growth inhibition bioassay may be useful to provide the comparative evaluation of toxicity of CPs in vitro, but also implicate that CP, DCP, TCP, in comparison with PCP, can induce L929 cell death by apoptosis, resulting in lower procarcinogensis, which may help to elucidate the molecular basis for the adverse health effects associated with CPs exposure.
Apoptosis, chlorophenol chemicals, cytotoxicity, fibroblast cells, structure-activity relationship, tumor promotion
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