李云庆
主要从事痛信息传递和调控机理的研究。
个性化签名
- 姓名:李云庆
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:-
-
学科领域:
神经病学
- 研究兴趣:主要从事痛信息传递和调控机理的研究。
暂无
-
主页访问
2977
-
关注数
0
-
成果阅读
547
-
成果数
10
李云庆, Y.-Y. Wang, S.-X. Wu, X.-Y. Liu, W. Wang, and Y.-Q. Li*
Biochemical and Biophysical Research Communications 309(2003)631-636,-0001,():
-1年11月30日
Effects of c-fos antisense oligodeoxynucleotide (ASO) on serotonin (5-HT)-induced upregulation of preprodynorphin (ppDyn), preproenkephalin (ppEnk), and glutamic acid decarboxylase (GAD), a special chemical marker for c-aminobutyric acid (GABA) neurons, mRNAs in cultured spinal dorsal horn neurons were investigated in order to extend our understanding of expressions of opioid peptides and GABA in spinal cord regulated by the descending serotonergic efferents. Reverse transcription-polymerase chain reaction analysis revealed a time-course increase in the expression of mRNAs encoding c-fos, ppDyn, ppEnk, and GAD after administration of 5-HT (100 nM). Administration of c-fos ASO (0.02 nM) 30 min prior to 5-HT application markedly blocked the expression of c-fos gene. Moreover, c-fos ASO pretreatment significantly decreased the 5-HT-induced upregulation of ppDyn and ppEnk mRNAs, but failed to affect the expression level of GAD mRNA. These results suggest that the serotoningic raphe-spinal efferents might play an important role in regulating the synthesis of enkephalin, dynorphin, and GABA in the spinal dorsal horn neurons. The immediate early oncogene c-fos might be involved in the 5-HT-induced increase in ppDyn and ppEnk expression. However, under the present experimental conditions, c-fos does not seem to be associated with the upregulation of GAD mRNA induced by 5-HT.
c-fos, Antinociception, Opioid peptides, Glutamic acid decarboxylase, Antisense oligodeoxynucleotide, Spinal cord
-
62浏览
-
0点赞
-
0收藏
-
0分享
-
148下载
-
0评论
-
引用
【期刊论文】5-Hydroxytryptamine1A receptor is involved in the bee venom induced inflammatory pain
李云庆, Wen Wang, Sheng-Xi Wu, Ya-Yun Wang, Xiang-Yu Liu, Yun-Qing Li*
Pain 106(2003)135-142,-0001,():
-1年11月30日
Injection of bee venom into one hindpaw of rat can elicit acute inflammation together with spontaneous pain, heat hyperalgesia and mechanical hyperalgesia/allodynia in the injected paw. 5-Hydroxytryptamine (5-HT)1A receptor is the predominant receptor subtype in the spinal dorsal horn mediating the function of 5-HT in nociception. The goal of the present study is to assess the role of 5-HT1A receptor in the pain associated with the bee venom induced inflammation. Here we showed that 1 or 4 h after a subcutaneous bee venom challenge, expression of 5-HT1A receptor mRNA in the ipsilateral lumbar spinal cord increased significantly by 80.94 or 37.86%, respectively. Antisense oligodeoxynucleotide knockdown of spinal 5-HT1A receptor attenuated spontaneous pain and reversed heat hyperalgesia in rats injected with bee venom. Thus, the present data suggest a facilitating role for 5-HT1A receptor in bee venom induced inflammatory pain.
5-Hydroxytryptamine1A receptor, Bee venom, Antisense oligodeoxynucleotide, Spontaneous pain, Hyperalgesia, Rat
-
49浏览
-
0点赞
-
0收藏
-
0分享
-
136下载
-
0评论
-
引用
李云庆, Dan Wang, Jian-Hong Wu, Yuan-Xiang Dong, Yun-Qing Li*
Brain Research 921(2001)133-137,-0001,():
-1年11月30日
The synaptic connections between g-aminobutyric acid (GABA)- and glycine-immunoreactive terminals and neurons projecting to the lateral parabrachial region were examined by a combination of retrograde tracing and immunohistochemical staining in the rat medullary dorsal horn. After injection of horseradish peroxidase (HRP) into the right lateral parabrachial region, HRP retrogradely labeled neurons were observed bilaterally in laminae I, II and III of the medullary dorsal horn with an ipsilateral predominance. GABA- and glycine-like immunoreactive terminals were found in laminae I, II and III. Some of these GABA- and glycine-like immunoreactive terminals were observed chiefly to make symmetric synapses with HRP-labeled neuronal cell bodies and dendritic processes. The present results indicate that neurons in the medullary dorsal horn projecting to the lateral parabrachial region might be modulated by GABAergic and glycinergic inhibitory intrinsic neurons, which might be significantly involved in the regulation of the noxious information transmission.
g-Aminobutyric acid, Glycine, Medullary dorsal horn, Parabrachial nucleus, Electron microscopy, Rat
-
56浏览
-
0点赞
-
0收藏
-
0分享
-
121下载
-
0评论
-
引用
李云庆, YUN-QING LI, HUI LI, KUN YANG, ZHI-MING WANG, TAKESHI KANEKO, AND NOBORU MIZUNO*
THE JOURNAL OF COMPARATIVE NEUROLOGY 428: 641-655 (2000),-0001,():
-1年11月30日
Morphology and electrical membrane properties of neurons in the superficial part of the magnocellular layer of the rat medullary dorsal horn (MDH: caudal subnucleus of the spinal trigeminal nucleus) were examined by using horizontal slice preparations. Intracellular recording and biocytin-injection combined with histochemical and immunohistochemical staining were done. Twenty-four neurons were examined successfully and classified into projection neurons (PNs) and intrinsic neurons (INs). The PNs were further divided into type I PNs (I-PNs) and type II PNs (II-PNs). The I-PNs sent axons to the medullary reticular formation; the II-PNs sent axons to the interpolar subnucleus of the spinal trigeminal nucleus but had no axons extending to the medullary reticular formation. The INs that sent no axons to the brain regions outside the MDH were also divided into small INs with spiny dendrites (INSSs) and large INs with aspiny dendrites (INLAs). The dendritic fields of the PNs extended to laminae I and II of the MDH and occasionally further to the spinal tract of the trigeminal nerve, whereas those of the INs were confined within the magnocellular layer of the MDH. The axonal branches of each IN formed a dense axonal mesh around the cell body of the parent neuron. Although the main bodies of the axonal fields of the INs were located in the magnocellular layer, some axonal branches extended to laminae I and II of the MDH. Immunoreactivity for NK1 receptor (substance P receptor) was found in approximately half of the PNs but not in the INs. Although no strong correlation was found between morphology and electrical membrane properties, there were some differences in electrical properties among the morphologically classified neuron groups, e.g., hyperpolarizing sag was observed in some PNs but not in the Ins; inward rectification was observed in some of the INSSs and INLAs but not in the PNs; the slow ramp depolarization and the slow afterdepolarization were observed in all INSSs examined but not in the PNs or INLAs. J. Comp. Neurol. 428: 641-655, 2000.
nociception, spinal trigeminal nucleus, somatosensory system, NK1 receptor, slice preparation, intracellular recording
-
45浏览
-
0点赞
-
0收藏
-
0分享
-
165下载
-
0评论
-
引用
李云庆, YUN-QING LI, HUI LI, TAKESHI KANEKO, AND NOBORU MIZUNO*
THE JOURNAL OF COMPARATIVE NEUROLOGY 411: 399-412 (1999),-0001,():
-1年11月30日
Morphologic features and electrical membrane properties of neurons in the substantia gelatinosa (SG) of the caudal spinal trigeminal nucleus (the medullary dorsal horn; MDH) were examined in the rat. Intracellular recording and biocytin-injection combined with histochemical staining were performed in horizontal slices. Twenty-four SG (lamina Ⅱ) neurons were recorded stably and stained successfully. Both projection neurons (PNs; n 5 9) that sent axons to regions outside the MDH and intrinsic neurons (INs; n 5 15) that sent axons only to the MDH were observed. The INs were divided into those with dense axonal arborization (INDAs; n 5 7) and those with sparse axonal arborization (INSAs; n 5 8). In the PNs, the dendrites with spines spread to all MDH layers (laminae Ⅰ-Ⅲ). The main axons sent collaterals within the SG and rostrally, caudally, or medially to laminae I and III of the MDH, interpolar spinal trigeminal nucleus, spinal tract of the trigeminal nerve, or upper cervical cord segments. In the INDAs, the dendrites arising from the rostral and caudal pole of the cell bodies mainly extended rostrally and caudally parallel to the rostrocaudal axis of the SG: the dendritic trees were elongated and oval in shape and were confined within the SG. The axonal field of each INDA, a dense mesh of axonal processes, was elongated and oval in shape and almost was confined within the SG. In the INSAs, a small, round cell body was located in the center of each dendritic field, which usually was limited within the SG. Axonal processes ran radially to spread to all layers of the MDH, constituting round or oval axonal fields. The three groups of SG neurons showed more or less different intracellular responses to current injections. In particular, adaptation of spike frequency, hyperpolarizing sag, and rebound excitation were observed in the PNs and INSAs but not in the INDAs. Slow ramp depolarization and slow afterdepolarization were recorded only in INDAs. J. Comp. Neurol. 411: 399-412, 1999.
pain, spinal trigeminal nucleus, somatosensory system, slice preparation, intracellular recording
-
85浏览
-
0点赞
-
0收藏
-
0分享
-
262下载
-
0评论
-
引用
李云庆, YUN-QING LI, HUI LI, KUN YANG, TAKESHI KANEKO, AND NOBORU MIZUNO*
THE JOURNAL OF COMPARATIVE NEUROLOGY 424: 24-36 (2000),-0001,():
-1年11月30日
Possible correspondence between morphologic features and electrical membrane properties of projection neurons in lamina I [the marginal zone (MZ)] of the caudal subnucleus of the spinal trigeminal nucleus [the medullary dorsal horn (MDH)] was examined by using intracellular recordings and biocytin-injections combined with histochemical and immunohistochemical staining techniques. The experiments were done in horizontal slice preparations of the rat brain. Thirteen MZ neurons were recorded stably and stained successfully. These neurons were confirmed to send their axons to the brain regions outside theMDHby camera lucida reconstruction. They were divided into two types on the basis of branching patterns of their axons within the MDH: Type I projection (P-I) neurons (n 5 7 neurons) had main axons that rarely emitted axon collaterals within the MDH, whereas type II projection (P-II) neurons (n 5 6 neurons) had main axons that emitted many axon collaterals within laminae I, II (substantia gelatinosa), and III (magnocellular part) of the MDH and also to the spinal tract of the trigeminal nerve; these axon collaterals usually constituted a dense mesh of axonal processes within laminae I and II of the MDH, especially in lamina II. About half of the neurons of each type showed immunoreactivity for the neurokinin-1 receptor. Resting membrane potentials were significantly more positive in P-I neurons than in P-II neurons. The P-II neurons had higher input resistance, a longer membrane time constant, and a higher threshold for spike than P-I neurons. In response to weak, long depolarizing current pulses, P-II neurons often showed slow ramp depolarization; the same neurons exhibited delayed repolarization to the resting potential (slow afterdepolarization) after the offset of the long depolarizing current pulses. Neither the slow-ramp depolarization nor the slow afterdepolarization was observed in P-I neurons. Slow return to resting membrane potential after offset of hyperpolarizing current pulses also was observed frequently in P-II neurons but not in P-I neurons. The results indicate that P-II neurons differ in their membrane properties compared with P-I neurons, and P-II neurons may be involved in the local circuit mechanism within the MDH more deeply than P-I neurons. J. Comp. Neurol. 424: 24-36, 2000.
nociception, spinal trigeminal nucleus, somatosensory system, neurokinin-1 receptor, slice preparation, intracellular recording
-
61浏览
-
0点赞
-
0收藏
-
0分享
-
81下载
-
0评论
-
引用
李云庆, YUN-QING LI, MASAHIKO TAKADA, TAKESHI KANEKO, AND NOBORU MIZUNO*
THE JOURNAL OF COMPARATIVE NEUROLOGY 378: 283-294 (1997),-0001,():
-1年11月30日
The distribution of inhibitory premotor neurons for the facial and hypoglossal nuclei was examined in the lower brainstem of the rat. A retrograde axonal tracing method with the fluorescent tracer, tetramethylrhodamine dextran amine (TMR-DA), was combined with immunofluorescence histochemistry for glutamic acid decarboxylase (GAD), i.e., the enzyme involved in gamma-aminobutyric acid synthesis, or glycine. In the rats injected with TMR-DA unilaterally into the facial or hypoglossal nucleus, the distribution of TMR-DA-labeled neurons showing GAD-like immunoreactivity (GAD/TMR-DA neurons) was essentially the same as that of TMR-DA-labeled neurons displaying glycine-like immunoreactivity (Gly/ TMR-DA neurons). The distributions of GAD/TMR-DA and Gly/TMR-DA neurons in the rats injected with TMR-DA into the facial nucleus were also similar to those in the rats injected with TMR-DA into the hypoglossal nucleus. These neurons were seen most frequently in the lateral aspect of the pontine reticular formation, the supratrigeminal region, the dorsal aspect of the lateral reticular formation of the medulla oblongata, and the reticular regions around the raphe magnus nucleus and the gigantocellular reticular nucleus pars alpha, bilaterally with a slight dominance on the side ipsilateral to the injection site.Anumber of GAD/TMR-DA and Gly/TMR-DA neurons were also seen in the oral and interpolar subnuclei of the spinal trigeminal nucleus, bilaterally with a slight ipsilateral dominance. In the rats injected with TMR-DA into the facial nucleus, GAD/TMR-DA and Gly/TMR-DA neurons were also encountered in the paralemniscal zone of the midbrain tegmentum bilaterally with an apparent dominance on the side contralateral to the injection site. A large part of these inhibitory premotor neurons for the facial and hypoglossal nuclei and the excitatory ones may constitute premotor neuron pools common to the orofacial motor nuclei implicated in the control of integrated orofacial movements. J. Comp. Neurol. 378: 283-294, 1997.
face, tongue, reticular formation, brainstem, immunohistochemistry
-
53浏览
-
0点赞
-
0收藏
-
0分享
-
115下载
-
0评论
-
引用
李云庆, Yun-Qing Li a, Hui Li a, Takeshi Kaneko b, Noboru Mizunoc, *
Brain Research 900(2001)110-118,-0001,():
-1年11月30日
The morphology and electrophysiological properties of serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleus (DRN) of the rat were examined in frontal brain slices. Biocytin was injected intracellularly into the intracellularly recorded neurons. Then the morphology of the recorded neurons was observed after histochemical visualization of biocytin. The recorded neurons extending their main axons outside the DRN were considered as projection neurons. Subsequently, serotonergic nature of the neurons was examined by serotonin (5-HT) immunohistochemistry. The general form of the dendritic trees is radiant and poorly branching in both 5-HT- and non-5-HT neurons. However, the dendrites of the 5-HT neurons were spiny, whereas those of the non-5-HT neurons were aspiny. The main axons of both 5-HT- and non-5-HT neurons were observed to send richly branching axon collaterals to the DRN, ventrolateral part of the periaqueductal gray and the midbrain tegmentum. In response to weak, long depolarizing current pulses, the 5-HT neurons displayed a slow and regular firing activity. The non-5-HT neurons fired at higher frequencies even when stronger current was injected. Some other differences in electrophysiological properties were also observed between the 5-HT-immunoreactive spiny projection neurons and the 5-HT-immunonegative aspiny projection neurons.
Dorsal raphe nucleus, Serotonin, Intracellular recording, Intracellular staining, Immunohistochemistry, Rat
-
38浏览
-
0点赞
-
0收藏
-
0分享
-
124下载
-
0评论
-
引用
李云庆, Hui Li, Le Wu, and Yun-Qing Li*
Biochemical and Biophysical Research Communications 307(2003)730-736,-0001,():
-1年11月30日
The modulatoryeffects of methionine-enkephalin (M-ENK) and selective opioid-receptor agonists on GABA-activated wholecell currents were investigated in neurons acutelydissociated from the superficial laminae of the rat spinal dorsal horn using nystatin-perforated patch recording configuration under voltage-clamp conditions. The results show that: (1) GABA acted on GABAA receptors and elicited inward Cl currents (IGABA) at)60mV; (2) M-ENK depressed IGABA in 65% of the tested neurons and potentiated IGABA in 15% of the neurons tested; (3) the agonists of l-, j-, and d-opioid receptors-[D-AIa2,N-Me-Phe4,Gly5-ol]-enkephalin (DAMGO), dynorphin-A (Dyn-A), and [D-Pen2,D-Pen5]-enkephalin (DPDPE) also depressed the IGABA, and the order of agonist potencywas DAMGO>Dyn-A>DPDPE; and (4) naloxone blocked the inhibitoryeffects of M-ENK on IGABA. The antagonists of l-, j-, and d-opioid receptors-b-funaltrexamine (b-FNA), nor-binaltorphimine (nor-BNI), and naltrindole (NTI) prevented the DAMGO-, Dyn-A-, and DPDPE-induced depression of IGABA. The results suggest that M-ENK downregulates IGABA principallythrough l-and j-opioid receptors, and thus exerts its modulating effects indirectlyon the transmission of noxious information at the spinal level.
Enkephalin, Spinal dorsal horn, Opioid receptor, GABA, Rat
-
53浏览
-
0点赞
-
0收藏
-
0分享
-
77下载
-
0评论
-
引用
李云庆, Yu-Peng Feng, Kun Yang, Yun-Qing Li*
Neuroscience Letters 322(2002)99-102,-0001,():
-1年11月30日
Capsaicin selectively excites nociceptive primary afferent fibers and increases the frequency of glutaminergic miniature excitatory postsynaptic currents (mEPSCs) in the substantia gelatinosa of the spinal dorsal horn. The whole-cell voltage-clamp recording technique was used to examine the effect of norepinephrine (NE) on the capsaicin-induced increase in the frequency of mEPSCs. In the presence of tetrodotoxin, bath application of capsaicin (1 mM) remarkably enhanced the frequency of mEPSCs (295 ^ 52% of control). Following pretreatment with NE (10 mM), the capsaicininduced frequency facilitation of mEPSCs was significantly depressed to 151
Norepinephrine, Capsaicin, Substantia gelatinosa, Miniature excitatory postsynaptic currents, Nociception, Rat
-
45浏览
-
0点赞
-
0收藏
-
0分享
-
98下载
-
0评论
-
引用