韩召军
害虫抗药性靶标分子生物学研究,结合农业生产,进行害虫抗药性监测、研究。
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- 姓名:韩召军
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学术头衔:
博士生导师,
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学科领域:
植物保护学
- 研究兴趣:害虫抗药性靶标分子生物学研究,结合农业生产,进行害虫抗药性监测、研究。
韩召军,男,1957年8月生,1982年1月获南京农业大学学士,1984年12月获南京农业大学硕士学位,1990年12月获南京农业大学博士学位。1985年1月留校任昆虫学助教,1987年晋升讲师,1992年晋升副教授,1997年晋升教授。1993年赴英国自然资源所昆虫系合作研究3个月,1995-1997赴英国洛桑试验站昆虫分子毒理实验室客座研究2年,2001-2002赴美国加州大学河边分校昆虫分子遗传实验室访问研究半年。现任植保学院院长、农业部重点实验室主任、中国昆虫学会副理事长、江苏省昆虫学会理事长。先后主持国家948技术引进项目1项、国家自然科学基金项目3项、国家攻关项目专题1项,江苏省自然科学基金和基础研究项目3项,省重点攻关项目1项。参加国家973基础研究项目和863高新技术项目各1项。进行害虫抗药性靶标分子生物学研究,揭示了棉蚜对拟除虫菊酯和有机磷抗性、棉铃虫对有机磷抗性、二化螟对杀虫单抗性和褐飞虱对有机磷及吡虫啉抗性的分子机理,克隆了13个新的全基因序列。其中从棉蚜中克隆得到的2个乙酰胆碱酯酶基因,首次证实了昆虫中存在不同乙酰胆碱酯酶基因的假设,并为研究乙酰胆碱酯酶抑制剂的毒理学、开发高选择性绿色化学农药提供了基础。结合农业生产,进行害虫抗药性监测、研究害虫抗药性机制和交互抗性谱,开发高效杀虫剂,研究害虫抗药性田间发生规律,建立有效的抗药性治理配套技术,在抗性棉铃虫和抗性二化螟的治理中发挥了巨大作用,并产生了可观的经济效益和生态效益。先后发表论文60余篇,其中SCI论文9篇。获国家科技进步二等奖1项,农业部科技进步一等奖1项,江苏省科技进步二等奖1项,国家教委科技进步三等奖1项,江苏省教委科技进步二等奖1项。先后被评为江苏省优秀青年科技骨干、“333”人才工程第一层次培养对象、农业部突贡专家、政府特别津贴获得者。
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韩召军, 李飞, 韩召军*, 唐波
昆虫学报 Acta Entomologica Sinica, October 2003, 46 (5): 578-583,-0001,():
-1年11月30日
用浸叶法测定了采自我国不同地区(泰安、莱阳、南京、北京和安阳)的棉蚜品系Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ对久效磷、甲胺磷、抗蚜威和灭多威等杀虫剂的抗性水平,各棉蚜品系对杀虫剂的抗性依次为Ⅴ>Ⅳ>Ⅲ,Ⅱ>Ⅰ。进一步研究表明,Ⅴ和Ⅳ品系棉蚜乙酰胆碱酯酶对杀虫剂的敏感性显著下降,Ⅱ品系次之,Ⅲ和Ⅰ品系接近于敏感品系。Ⅴ和Ⅳ品系乙酰胆碱酯酶的Km值显著下降,表明酶发生了质的变化。不同棉蚜抗性品系的酯酶(全酯酶和羧酸酯酶)活性均显著升高,其中Ⅲ品系的酯酶活力为Ⅱ品系的2倍。Ⅴ品系羧酸酯酶Km值达2460.4μmol/L,而Ⅳ品系仅为84.4μmol/L,该两个品系羧酸酯酶发生了质的变化。研究结果表明,不同抗性程度的棉蚜品系均存在代谢抗性和靶标抗性。低抗水平的棉蚜品系,以代谢抗性为主,靶标抗性为辅;中抗水平的棉蚜品系,抑或由于解毒代谢酶的活性显著增强,也可能由于靶标的敏感性显著下降;而高抗水平的棉蚜品系,依赖于代谢抗性和靶标抗性的联合作用。
棉蚜, 抗性, 乙酰胆碱酯酶, 羧酸酯酶, 抑制中浓度, 米氏常数
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【期刊论文】Two different genes encoding acetylcholinesterase existing in cotton aphid (Aphis gossypii)
韩召军, Fei Li and Zhao-Jun Han
Genome 45: 1134-1141 (2002),-0001,():
-1年11月30日
ated by the PHYLIP program package inferred that AChE2 of A. gossypii is a more ancestral form of AChE. Homology modeling of structures using Torpedo californica (2ACE_) and Drosophila melanogaster (1Q09:A) native acetylcholinesterase structure as main template indicated that the two AChEs of Aphis gossypii might have different three-dimensional structures. Alternative splicing of Ace1 near the 5’-end resulting in two proteins differing by the presence or absence of a fragment of four amino acids is also reported.
Aphis gossypii Glover,, acetylcholinesterase,, gene clone,, homology modeling,, alternative splicing,, phylogenetic analysis.,
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【期刊论文】Triazophos resistance mechanisms in the rice stem borer (Chilo suppressalis Walker)
韩召军, Qu Mingjing, Han Zhaojun, *, Xu Xinjun, and Yue lina
M. Qu et al. / Pesticide Biochemistry and Physiology 77(2003)99-105,-0001,():
-1年11月30日
A field population of the rice stem borer (Chilo suppressalis Walker) with 203.3-fold resistance to triazophos was collected. After 8-generation of continuous selection with triazophos in laboratory, resistance increased to 787.2-fold, and at the same time, the resistance to isocarbophos and methamidophos was also enhanced by 9- and 4-fold, respectively, implying some cross-resistance between triazophos and these two organophosphate insecticides. Resistance to abamectin was slightly enhanced by triazophos selection, and fipronil and methomyl decreased. Synergism experiments in vivo with TPP, PBO, and DEM were performed to gain a potential indication of roles of detoxicating enzymes in triazophos resistance. The synergism results revealed that TPP (SR, 92) and PBO (SR 63) had significant synergistic effects on triazophos in resistant rice borers. While DEM (SR 0.83) showed no effects. Assays of enzyme activity in vitro demonstrated that the resistant strain had higher activity of esterase and microsomal O-demethylase than the susceptible strain (20- and 30-fold, respectively). For glutathione S-transferase activity, no difference was found between the resistant and the susceptible strain when DCNB was used as substrate. However, 28-fold higher activity was observed in the resistant strain when CDNB was used. These results showed that esterase and microsomal-O-demethylase play some roles in the resistance. Some iso-enzyme of glutathione S-transferase may involve in the resistance to other insecticides, for this resistant strain was selected from a field population with multiple resistance background. Acetylcholinesterase as the triazophos target was also compared. The results revealed significant differences between the resistant and susceptible strain. The Vmax and Km of the enzyme in resistant strain was only 32 and 65% that in the susceptible strain, respectively. Inhibition tests in vitro showed that I50 of triazophos on AChE of the resistant strain was 2.52-fold higher. Therefore, insensitive AChE may also involved in triazophos resistance mechanism of rice stem borer.
Chilo suppressalis Walker, Triazophos, Cross-resistance, Resistance mechanism
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韩召军, Zhaojun Han, , Graham D. Moores, Ian Denholm, and Alan L. Devonshire
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 62, 164-171 (1998),-0001,():
-1年11月30日
The activity and electrophoretic mobility of nonspecific esterases, and the sensitivity of acetylcholinesterase (AChE) to organophosphate and carbamate insecticides, was determined for 21 strains or clones of Aphis gossypii of worldwide origin and related to patterns of insecticide resistance disclosed by diagnostic dose bioassays with demeton-S-methyl, pirimicarb, and permethrin. Results confirmed previous work showing the occurrence of two forms of mutant AChE, both conferring insensitivity to pirimicarb and representing the primary mechanism of resistance to this insecticide. One of these mutant AChE enzymes also caused resistance to demeton-S-methyl in its own right, whereas the other only conferred resistance in combination with moderate or high levels of nonspecific esterase activity. Lack of any correlation between permethrin resistance and quantitative or qualitative changes in esterases implied a distinct, as yet uncharacterised, mechanism of resistance to pyrethroids, possibly based on insensitivity in the target protein for these pesticides.
Aphis gossypii, insecticide resistance, esterase, acetylcholinesterase.,
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韩召军, Liu Zewen, Han Zhaojun, *, Wang Yinchang, Zhang Lingchun, Zhang Hongwei and Liu Chengjun
,-0001,():
-1年11月30日
A field population of brown planthoppers (Nilaparvata lugens St
Nilaparvata lugens, resistance mechanism, cross-resistance, synergism, P450-monooxygenases, acetylcholine receptors
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【期刊论文】Dynamics of pyrethroid resistance in a field population of Helicoverpa armigera (H
韩召军, Zhaojun Han, *, Yinchang Wang, Qiansong Zhang, Xianchun Li and Guoqing Li
Pestic Sci 55: 462-466 (1999),-0001,():
-1年11月30日
Dynamics of pyrethroid resistance in a field population of cotton bollworm (Helicoverpa armigera) was demonstrated by continuous monitoring with twin discriminating dosages, and the influencing factors were also experimentally analysed. Resistance in a field population in China increased rapidly in the 3rd and 4th generations when population density became higher and insecticides were applied repeatedly, then decreased suddenly during over-wlntering and slowly in the 1st and 2nd generations when insecticide spraying was suspended. Resistance increase could be countered by dilution as a result of immigration of susceptible moths from corn fields, which were found to be a natural refuge for this pest in China, The reduction of resistance during over-wintering and the 1st and 2nd generations was affected by the lower fitness of resistant cotton bollworms to low temperature and disadvantages in reproduction. The possibilities of managing the resistance in field populations on the basis of these observations are discussed.
pyrethroid, resistance, field population, Helicoverpa armigera
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韩召军, Li Guoqing*, Han Zhaojun, Mu Lili, Qin Xiaoran, Chen Changkun, Wang Yinchang
Journal of Insect Physiology 47(2001)951-956,-0001,():
-1年11月30日
Observation of the oviposition behavior of cotton bollworm demonstrated the existence of substances that can act as oviposition deterrents. The scales, abdomens and tarsi of the moths, the most likely carrier, were extracted for the substances. An acetone extract exhibiting an oviposition deterrent effect was analyzed using gas chromatography and combined gas chromatography-massspectroscopy. Three components, 4-methyl-4-hydroxyl-pentanone-2, hexadecanoic acid and (Z)-9-octadecenoic acid, were identified, being present in the ratio 1:2:2. Experiments with synthetic mixtures indicated that a mixture of equal amounts of the two organic acids exhibited similar repellent and deterrent activity to a synthetic mixture of all three components. It is concluded that the natural oviposition-deterring pheromone is a mixture of hexadecanoic acid and (Z)-9-octadecenoic acid.
Helicoverpa armigera, Tarsus, Oviposition-deterring pheromone
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韩召军, Fei Li, Zhaojun Han*
Insect Biochemistry and Molecular Biology 34(2004)397-405,-0001,():
-1年11月30日
Two acetylcholinesterase genes, Acel and Ace2, have been fully cloned and sequenced from both oranophoshate-resistant and susceptible clones of cotton aphid. Comparison of both nucleic acid and deduced amino acid sequences revealed considerable nucleotide polymorphisms. Further study found that two mutations occurred consistently in all resistant aphids. The mutation F139L in Ace2 corresponding to F115S in Drosophila acetylcholinesterase might reduce the enzyme sensitivity and result in insecticide resistance. The other mutation A320S in Acel abutting the conserved catalytic triad might affect the activity and insecticide sensitivity of the enzyme. Phylogenetic analysis showed that insect acetylcholinesterases fall into two subgroups, of which Acel is the paralogous gene whereas Ace2 is the orthologous gene of Drosophila AChE. Both subgroups contain resistance-associated AChE genes. To avoid confusion in the future work, a nomenclature of insect AChE is also suggested in the paper.
Aphis gossypii Glover, Acetylcholinesterase, Single nucleotide polymorphism, Insecticide resistance
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【期刊论文】Mechanisms of Monocrotophos Resistance in Cotton Bollworm, Helicoverpa armigera (H
韩召军, Xiaoxia Ren, Zhaojun Han, * and Yinchang Wang
Archives of Insect Biochemistry and Physiology 51: 103-110 (2002),-0001,():
-1年11月30日
Insensitive acetylcholinesterase was identified as a esistance mechanism by comparing biochemical analysis with a laboratory selected monocrotophos resistant cotton bloodworm (RR: 200) and the susceptible strain The cDNA enCODING AChE was cloned by the method of RACE (rapid amplification of cDNA ends). The complete AChE gene deduced from the cDNA consisted of a putative signal peptide of 32 amino acid residues, a mature protein of 615 residues, 5' untranslated regions (UTR) of 315bp and 3' UTR of 324bp. The Coding sequence had a high degree of Homology to the AChE from other insect species reported in the GenBank. After comparing analysis of the entire AChE gene sequence from 5 resistant and 6 susceptible cotton bollworm individuals, nine mutations were identified. One of them, the Ala/Thr mutation, is likely to be responsible for the AChE insensitivity to monocrotophos.
cotton bollworm, insecticide resistance, acetylcholinesterase, gene mutation
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韩召军, Fei Li and Zhaojun Han*
Archives of Insect Biochemistry and Physiology 51: 37-45 (2002),-0001,():
-1年11月30日
gossypii Glover. The procedure involved filtration on a sephadex G-25 column, separation with sephadex G-200 and procainamide offinity column. AChE from both susceptible and resistant strains were purified to a single band as resolved on denaturing polyacrylamide gel electrophoresis (SDS-PAGE). The specific activity increased by 35, 100- and 33, 680-fold with a yield of 30.3 and 29.8%, respectively. The molecular mass of the purified AChE was about 63, 500 Dalton as etermined by SDS-PAGE. However, three bands resolved on PAGE gel electrophoresis, leading to the inference that native AChE exists in three forms. The optimum conditions for measuring the activity of purified AChE with kinetic method were 0.02M phosphate buffer, pH7.2, 0.02mM 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), AND 25℃. Investigation also revealed that crude extract and purified AChE had different kinetic characteristics and inhibiroty properties. They responded differently to varied DTNB, ATChI, and phosphate buffer ion concentrations, as well as pH, temperature, and inhibitors. The purified AChE was more sensitive to eserine, methamidophos, and pirimicarb. Especially for resistant aphids, the sensitivity of purified AChE to methamidophos and pirimicarb was enhanced 6.43 and 11.73 times, respectively. We infer that one or more factors in the crude extract from the resistance stain have more influence on AChE sensitivity. Further study is needed to investigate the basis of these observations.
Aphis gossypii Glover, acetylcholinesterase, purification, procainamide affinity column, kinetic characterization, inhibition
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