张景强
病毒形态学和细胞超微结构研究
个性化签名
- 姓名:张景强
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学术头衔:
博士生导师
- 职称:-
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学科领域:
光学
- 研究兴趣:病毒形态学和细胞超微结构研究
张景强,1961年中山大学物理系毕业。留物理系工作两年后,1963年调往生物系筹建电子显微学实验室。此后一直在该室和生物防治国家重点实验室结构生物学研究室工作至今。现为中山大学生科院生物物理学教授,博士生导师。
20世纪70年代从事病毒形态学和细胞超微结构研究。80年代起,把计算机图像处理和高分辨电子显微技术引进生物大分子的研究中去。首先开展蛋白电子晶体学研究,率先在国内建立起蛋白质电子晶体学的设备和技术,并用它获得分辨率为0.7nm的牛肝过氧化氢酶的晶体结构。此后,根据学科发展,开展病毒三维结构与功能的研究。在国内首先建立起高分辨冷冻电镜单颗粒技术和设备,并结合分子生物学技术对几种病毒的结构与功能进行研究,获得分辨率0.8nm的家蚕质多角体病毒(BmCPV)、2.5nm中蜂幼虫囊肿病毒(CSBV)和2.5nm的登革病毒的三维结构。证实BmCPV是以新的模式――整体穿膜进入受体细胞。在伊蚊C6/36细胞中首次发现潜伏有新病毒――伊蚊C6/36细胞浓核病毒(C6/36 DNV),并对其三维结构进行研究,获得1.0 nm的分辨率。2003年参与抗非科技攻关,并与广东CDC合作首先分离出SARS CoV广东株,并对其形态发生以及在Vero E6细胞中入侵-复制-溢出的生活周期进行研究。
曾到日本、美国、荷兰、墨西哥和新加坡等地学习、访问和参加学术会议。发表130多篇论文,著作两本。
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【期刊论文】伊蚊C6/36细胞浓核病毒蛋白衣壳三维结构的测定串
张景强, 程凌鹏①, 陈森雄①, Jenifer Brannan②, Joanita Jakana③, 张勤奋①, 周正洪②张景强①**
中国科学C辑生命科学2004,34 (1): 75~79,-0001,():
-1年11月30日
利用冷冻电子显微技术和计算机图像三维重构方法获得伊蚊C6/36细胞浓核病毒蛋白衣壳的三维结构,分辨率为1.4nm。结果显示C6/36DNV蛋白衣壳的三角形剖分数T=1,在二十面体的每个面上有12个孔,可能是DNA的通道,每个五次轴上有一个柱状突起。
冷冻电子显微技术, 三维重构, 伊蚊C6/, 36细胞浓核病毒
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张景强, 余学奎, 卢英, 张红, 周正洪, 张勤奋, 张兴, 张景强**
ACTA BIOCHIMICA et BIOPHYSICA SINICA 1999, 31(5):563-566,-0001,():
-1年11月30日
用负染和冷冻电镜技术以及计算机数据处理方法,研究了CPV完整和空病毒的结构。完整病毒和空病毒的结构和生化组成比较表明,CPV具有单层衣壳结构,病毒的5种结构蛋白都位于该单层衣壳上。该单层衣壳按T=1的对称结构排列,在二十面体的顶点具有塔状突起。空病毒与完整病毒具有相同的衣壳结构,但内部结构却不相同。完整病毒内部为致密而有序排列的双链RNA,而空病毒内部却几乎没有电子密度,只在突起的底部具有12个向内伸展的电子密度,作者认为该电子密度属于CPV转录酶复合物结构。
质多角体病毒(, CPV), , 转录酶复合物, 冷冻电镜技术
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张景强, Senxiong Chen, a, Lingpeng Cheng, Qinfen Zhang, a Wei Lin, a Xinying Lu, Jennifer Brannan, b Z. Hong Zhou, b, * and Jingqiang Zhanga, *
Virology 318(2004)123-133,-0001,():
-1年11月30日
We report the isolation, sequencing, biochemical, and structural characterization of a previously undescribed virus in a chronically infected Aedes albopictus C6/36 cell line. This virus is identified as a new densovirus under the Densovirinae subfamily of the Parvoviridae based on its biological and morphologic properties as well as sequence homologies, and is tentatively designated A. albopictus C6/36 cell densovirus (C6/36 DNV). Analysis of the 4094 nt of the C6/36 DNV genome revealed that the plus strand had three large open reading frames (ORFs): a left ORF, a right ORF, and a mid-ORF (within the left ORF), whose potential coding capacities are 91.0, 40.8, and 41.2 kDa, respectively. The left ORF likely encodes the nonstructural protein NS-1, which contains NTP-binding and helicase domains. The right ORF likely encodes structural proteins, VP1 and VP2. Our analyses revealed that C6/36 DNV has a similar genomic organization and shares very high homology in nucleotide sequence and amino acid sequences with Aedes aegypti densovirus (AaeDNV) and A. albopictus densovirus (AalDNV), members of the genus Brevidensovirus of the Densovirinae. Similar to other densoviruses, C6/36 DNV has a different genomic organization and no recognizable sequence homology with viruses in the Parvovirinae. The three-dimensional (3D) reconstruction of the C6/36 DNVat 15.6-A
Brevidensovirus, Genomic organization, Overlap gene, Aedes albopictus, Aedes aegypti, Conserved motifs, Electron cryomicroscopy, Threedimensional Structure
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【期刊论文】Cytoplasmic Polyhedrosis Virus Structure at 8A
张景强, Z. Hong Zhou, , * Hong Zhang, Joanita Jakana, Xing-Ying Lu, and Jing-Qiang Zhang
Structure, Vol. 11, 651-663, June, 2003, 2003 Elsevier cience Ltd. All rights reserved.,-0001,():
-1年11月30日
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张景强, Qing Xia‡, Joanita Jakana§, Jing-Qiang Zhang¶, and Z. Hong Zhou‡ From the ‡
Vol. 278, No.2, Issue of January 10, pp. 1094-1100, 2003,-0001,():
-1年11月30日
Viruses in the family Reoviridae are capable of transcription within the intact capsids. As the only singleshelled and thus the simplest member of the Reoviridae, cytoplasmic polyhedrosis virus (CPV) provides an attractive system for studying endogenous transcription. We report the structures of the full and empty CPV determined at 13-
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【期刊论文】NOTES Visualization of Protein-RNA Interactions in Cytoplasmic Polyhedrosis Virus
张景强, H. ZHANG, J. ZHANG, X. YU, X. LU, Q. ZHANG, J. JAKANA, D. H. CHEN, , X. ZHANG, AND Z. H. ZHOU*
JOURNAL OF VIROLOGY,Feb. 1999, p. 1624-1629,-0001,():
-1年11月30日
Unlike the multiple-shelled organization of other Reoviridae members, cytoplasmic polyhedrosis virus (CPV) has a single-shelled capsid. The three-dimensional structures of full and empty CPV by electron cryomicroscopy show identical outer shells but differ inside. The outer surface reveals a T51 icosahedral shell decorated with spikes at its icosahedral vertices. The internal space of the empty CPV is unoccupied except for 12 mushroom-shaped densities attributed to the transcriptional enzyme complexes. The ordered double-stranded RNA inside the full capsid forms spherical shells spaced 25
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【期刊论文】The Life Cycle of SARS Coronavirus in Vero E6 Cells
张景强, Zhang Qinfen, Cui Jinming, Huang Xiaojun, Zheng Huanying, Huang Jicheng, Fang Ling, Li Kunpeng, and Zhang Jingqiang*
Journal of Medical Virology 73:332-337(2004),-0001,():
-1年11月30日
The aim of the study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and atured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells. J. Med. Virol. 73:332-337, 2004.
SARS coronavirus, life cycle, electron microscopy
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【期刊论文】Entry of Bombyx mori cypovirus 1 into midgut cells in vivo
张景强, Yu-Rong Tan, Jing-Chen Sun, Xin-Ying Lu, De-Ming Su and Jing-Qiang Zhang*
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-1年11月30日
In vivo entry of Bombyx mori cypovirus 1 (BmCPV-1) into silkworm midgut cells was studied by elec tron microscopy. Virions were observed adhering to the plasma membrane of microvilli of the columnar cells, embedding in the membrane, and settling themselves intact inside the microvilli. These behaviors suggested that intact BmCPV-1 virions enter columnar cells by means of direct penetration through the cell membrane. In addition, goblet cells, muscle cells and the hemocoele were also involved in early events of the virus infection. However, no replication of the virus had ever been detected in these invaded cells except for columnar cells.
Bombyx mori cypovirus 1,, in vivo,, intact virion,, direct penetration,, electron microscopy
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