姜平
动物传染病防制及兽医生物技术
个性化签名
- 姓名:姜平
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师, 国家“百千万”人才工程国家级人选
- 职称:-
-
学科领域:
畜牧科学、动物医学
- 研究兴趣:动物传染病防制及兽医生物技术
南京农业大学动物医学院教授,博士,博士生导师,预防兽医学系主任,农业部动物疫病诊断与免疫重点开放实验室副主任。国家重点学科预防兽医学优秀学科带头人;教育部优秀青年教师;江苏省“333”新世纪科学技术带头人(第二层次);江苏省高校“青蓝工程”中青年学术带头人。中国畜牧兽医学会动物传染病学分会、禽病学分会和微生态学分会理事;江苏省动物传染病防治研究会副理事长;中国科学院武汉病毒研究所兼职研究员。1986年毕业于南京农业大学兽医学专业,获学士学位;1989年毕业本校传染病与预防兽医学专业获硕士学位;1998年毕业本校传染病与预防兽医学专业,获博士学位;2000--2001年美国Rush医学中心从事博士后研究。1998年加拿大Guelph大学兽医学院进修合作科研。面向二十一世纪全国统编教材《兽医生物制品学》主编。先后主持18项国家、部省级研究课题。研究领域涉及动物传染病防制及兽医生物技术,主要研究成果包括:(1)猪繁殖和呼吸综合征病原学、分子流行病学、诊断技术和基因工程疫苗的研制;(2)猪圆环病毒2型流行病学和病原学;(3)传染性法氏囊病野毒株的主要结构蛋白基因的免疫特性、CpG寡核苷酸新型免疫佐剂的免疫增强作用;(4)Heparanse1基因转录调控机理。至今,培养毕业硕士生5名,现有在读博士生2名,硕士研究生19名。主编出版专著3部,发表论文近50篇,5篇被SCI收录。获部省级科技进步奖3项。
-
主页访问
2554
-
关注数
0
-
成果阅读
468
-
成果数
8
姜平, 简中友, 马志永, 蔡家利, 蔡宝祥
南京农业大学学报,1997,20(3): 82~86,-0001,():
-1年11月30日
在对国内某地区7个患病猪群流行病学调查的基础上,用Marc2.45细胞培养从4个猪群流产胎儿分离到3株导致细胞病变的病毒--J1、J2和J3株。它们对氯仿和热(56℃,1h)、甲醛、酸、碱均敏感。病毒粒子呈球状,直径30~80nm,负染后病毒粒子大小80~100nm,在Marc2145细胞质内增殖,52溴22'2脱氧尿核苷(BUDR)对其无抑制作用。结合血清学试验,鉴定3个毒株均为猪繁殖和呼吸综合征病毒(PRRSV),可能为美洲型PRRSV。
猪繁殖和呼吸综合征病毒, 分离, 鉴定
-
57浏览
-
0点赞
-
0收藏
-
0分享
-
257下载
-
0评论
-
引用
姜平, 陈溥言, 蔡宝祥, 董永毅, 姜志华
中国兽医学报,1999,19(2):121~123,-0001,():
-1年11月30日
应用RT-PCR技术,从我国东北和华北地区分离的2个猪繁殖和呼吸综合征病毒(PRRSV)毒株中扩增获得部分核衣壳蛋白基因。该基因片段长度与美洲型野毒株VR2332 RT-PCR扩增片段相同,均为433bp,长于欧洲型Lelystad毒株扩增片段长度(395bp)。此外,用另1对引物,从这2个分离毒株及VR2332毒株扩增获得部分GP5蛋白基因,其限制性酶切片段长度多态性分析结果相同或相似。该结果表明,我国不同地区流行的PRRSV可能属于同一毒株,并且来源于美洲。
猪繁殖和呼吸综合征病毒, 基因型, RT-PCR
-
131浏览
-
0点赞
-
0收藏
-
0分享
-
146下载
-
0评论
-
引用
【期刊论文】我国两个不同地区猪繁殖和呼吸综合征病毒分离株ORF5基因序列比例
姜平, 陈溥言, 姜志华, 蔡家利, 董永毅, 蔡宝祥
病毒学报,2000,16(1):70~73,-0001,():
-1年11月30日
To elucidate the characteristics of molecular epidemiology of PRRSV in China, the nucleotide sequence of open reading frame 5 (ORF5) coding regions from two wild PRRSV strains, J 1 and S1, isolated in northern and mid-eastern regions of China was determined by RT-PCR. Comparison showed that only 4 different bases between J 1 and S1 strains resulted in 3 amino acid (aa.) changes in their deduced proteins. There were 3 and 2 different aa. between the deduced aa. sequences of J 1, S1 strains and the American prototype ATCC virus VR2332 respectively, while there existed 16 and 15 aa. changes between the 2 isolates and the modified live vaccine strains derived from VR2332. The putative protein molecular weight, isoelect ric point, signal region, glycosylation site and possible transmembrane helices of ORF5 of each strains were similar to that of VR2332 strain. These result s showed that the two isolates belong to the same genotype and they may come from USA.
猪繁殖和呼吸综合征病毒(PRRSV), ORF5基因, 基因序列, J1毒株, S1毒株
-
42浏览
-
0点赞
-
0收藏
-
0分享
-
145下载
-
0评论
-
引用
【期刊论文】上海地区出现猪繁殖与呼吸综合征和2型猪圆环病毒混合感染
姜平, 李玉峰, 王先炜, 姜平*
中国兽医学报,2003,23(5):442~444,-0001,():
-1年11月30日
上海地区6个规模化猪场断奶后仔猪出现全身消耗性综合征,剖检的18头病仔猪都表现肺脏严重病变和淋巴结肿大出血。分别设计针对猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)N蛋白和2型猪圆环病毒(porcine circovirustype2,PCV-2)部分基因的特异性PCR引物,通过RT2PCR和PCR技术从患病猪肺脏组织均扩增出PRRSV和PCV-2的特异基因片段。结合临床症状和流行病学调查,证实上海地区出现PRRSV和PCV-2的混合感染。
PRRSV, PCV-2, PCR
-
51浏览
-
0点赞
-
0收藏
-
0分享
-
136下载
-
0评论
-
引用
【期刊论文】传染性法氏囊病病毒VP3结构蛋白基因在大肠杆菌中的高效表达
姜平, 陈溥言, 蔡宝祥, 杨奎, 郑明球
病毒学报,1998,14(3):279~283,-0001,():
-1年11月30日
The VP3 structural protein gene fragment of chinese IBDV was amplified by RT-PCR, and inserted into the expression vector p GEX-2TK. Restriction enzyme analysis and partial sequence analysis showed that the sequence of foreign gene cloned was the same as that of VP3 gene reported before. On the other hand, the gene was located in the BamHI/EcoRI site of this vector in the corrected open reading frame. The recombinant plasmid, p GETVP33, could stably express VP3 as glutathione s-tranferase (GST) fusion protein with high efficiency (up to 34% of the total bacterial soluble protein) in E. coli BL21 through IPTG induction. Chicken incoulatad with the total bacterial protein in oil adjuvant at 14 days of age could produce IBDV-specific antibody as measured by EL ISA and were partially protected against challenge with virulent IBDV.
VP3结构蛋白的基因,, 传染性法氏囊病病毒,, 高效表达
-
37浏览
-
0点赞
-
0收藏
-
0分享
-
183下载
-
0评论
-
引用
【期刊论文】IBDV南京野毒株VP2结构蛋白基因克隆与表达*
姜平, 陈溥言, 蔡宝祥
南京农业大学学报, 1996, 19 (4): 61~65,-0001,():
-1年11月30日
用微机辅助设计合成了一对引物,用于扩增传染性法氏囊病病毒(IBDV)中国地方株VP2基因片段。RT2PCR扩增出-1321bp的目的片段,分子杂交鉴定为V P2基因。扩增产物经双酶切后插入含噬菌体PRPL双强启动子的pCYTEXP1表达质粒,构建了VP2基因克隆pCVP21,经Dot2ELISA筛选出4个VP2表达阳性克隆。SDS2PA GE和W estern blot显示有-约32000的目的蛋白带,且能与IBDV阳性血清结合。
传染性法氏囊病病毒, 结构蛋白, 基因克隆, 基因表达, RT 2PCR
-
67浏览
-
0点赞
-
0收藏
-
0分享
-
81下载
-
0评论
-
引用
姜平, Xiaoquan Wang, AB Ping Jiang, AC Shovel Deen, B Jiaqiang Wu, A Xiaowen Liu, B and Jiarong XuA
AVIAN DISEASES 47: 1305-1312, 2003,-0001,():
-1年11月30日
The objective of the present study was to investigate the feasibility of a DNA vaccine and CpG oligodeoxynucleotide (ODN) to protect chickens against infectious bursal disease virus (IBDV) infection. Two plasmids DNA carrying VP2 genes of the very virulent (vv) strain of IBDV were constructed with reverse transcription-polymerase chain reaction and designated as pcDNA3.1-VP2 and pCI-VP2. The VP2 protein expressed in COS-7 cells transfected with the plasmid was confirmed by indirect immunofluorescence assay. Seven-day-old chickens were intramuscularly injected with the plasmids alone or plus commercial attenuated infectious bursal disease (IBD) vaccine or synthetic CpG ODN twice at weekly intervals. Chickens at 5 wk old were orally inoculated with vvIBDV strain 99J1 and observed for 7 days after challenge. Immunization with plasmid plus commercial attenuated IBD vaccine or CpG ODN conferred protection for 70%-80% of chickens, as evidenced by the absence of clinical signs, mortality, and atrophy in the cloacal bursa. About 25%-45% of chickens vaccinated with commercial attenuated IBD vaccine or pcDNA3.1-VP2 or pCI-VP2 plasmid alone had clinical signs and died after challenge. Furthermore, there were significantly different histopathologic lesion scores in the clocal bursae between the pcDNA3.1-VP2 or pCI-VP2 plus CpG or live vaccine and pcDNA3.1-VP2, pCI-VP2, or live vaccine vaccinated group. Enzyme-linked immunosorbent assay antibody titers in chickens vaccinated the constructs DNA plus live vaccine or CpG ODN were significantly higher than in those inoculated with the constructs or the live vaccine alone. These results suggest that coadministration of the constructed plasmid pcDNA3.1-VP2 or pCI-VP2 with CpG ODN or commercial attenuated IBD vaccine could protect chickens efficiently from direct vvIBDV challenge.
-
34浏览
-
0点赞
-
0收藏
-
0分享
-
113下载
-
0评论
-
引用
姜平, Ping Jiang‡, Aseem Kumar§, Joseph E. Parrillo§, Laurie A. Dempsey¶, Jeffrey L. Platt¶**‡‡, Richard A. Prinz‡, and Xiulong Xu‡§§
Vol. 277, No.11, Issue of March 15, pp. 8989-8998, 2002,-0001,():
-1年11月30日
Heparanase-1 (HPR1) is an endoglycosidase that specifically degrades the heparan sulfate chains of proteoglycan, a component of blood vessel walls and the extracellular matrix. Recent studies demonstrated that HPR1 expression is increased in a variety of malignancies and may play a critical role in tumor metastases. The HPR1 gene and its genomic structure have been recently cloned and characterized. To understand the mechanisms of HPR1 gene expression and regulation, we first mapped the transcription start site of the HPR1 gene and found that HPR1 mRNA was transcribed from the nucleotide position 101bp upstream of the ATG codon. A 3.5-kb promoter region of the HPR1 gene was cloned. Sequence analysis revealed that the TATA-less, GC-rich promoter of the HPR1 gene belongs to the family of housekeeping genes. This 3.5-kb promoter region exhibited strong promoter activity in two thyroid tumor cell lines. Truncation analysis of the HPR1 promoter identified a minimal 0.3-kb region that had strong basal promoter activity. Truncation and mutational analysis of the HPR1 promoter revealed three Sp1 sites and four Ets-relevant elements (ERE) significantly contributing to basal HPR1 promoter activity. Binding to the Sp1 sites by Sp1 and to the ERE sites by GA-binding protein (GABP) was confirmed by electrophoretic mobility shift assay and competition and supershift electrophoretic mobility shift assays. Cotransfection of Sp-and GABP-deficient Drosophila SL-2 cells with the HPR1 promoter-driven luciferase construct plus the expression vector encoding the Sp1, Sp3, or GABP gene induced luciferase gene expression. Mutation or truncation of the Sp1 or ERE sites reduced luciferase expression in both SL-2 cells and thyroid tumor cell lines. Coexpression of GABP/and Sp1 or Sp3 further increased luciferase reporter gene expression. Our results collectively suggest that Sp1 cooperates with GABP to regulate HPR1 promoter activity.
-
49浏览
-
0点赞
-
0收藏
-
0分享
-
68下载
-
0评论
-
引用