周世宁
研究工作主要在微生物酶及活性代谢物的研究, 并开展相应基因克隆和表达研究
个性化签名
- 姓名:周世宁
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学术头衔:
博士生导师,
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学科领域:
光学
- 研究兴趣:研究工作主要在微生物酶及活性代谢物的研究, 并开展相应基因克隆和表达研究
1946年9月生, 男, 教授,博士生导师1970,中山大学生物系本科毕业; 1979-1982,中山大学微生物分子遗传学研究生; 1984-1987,美国Illinois大学医学中心生化系进修(访问学者);1970- 1991,中山大学生物系生化微生物专业任助教、讲师;1992- 今,中山大学生命科学学院生化系副教授、教授; 1993- 2004,中山大学生化系副主任、主任;1990-2003,广东省微生物学会常务理事、副理事长;2003-今, 广东省生物化学学会理事,1996- 今, 广州市微生物学会常务理事; 2001- 今, 教育部国家高等学校教学指导委员会地球科学委员会,海洋分会委员.大学毕业后即从事微生物遗传生化方面的研究和教学工作。参与建立了中山大学生化与微生物专业,以及广东省名牌专业中山大学生物技术专业, 从事过微生物、生化产品的研制和生产; 在美国工作期间从事微生物发育调控基因的结构与表达研究, 近年, 研究工作主要在微生物酶及活性代谢物的研究, 并开展相应基因克隆和表达研究。开展过芳香化合物分解细菌研究, 海洋细菌质粒与农药降解细菌的研究; 纯化了高温芽孢杆菌耐热木糖异构酶和海洋弧菌的几丁质酶;在真菌芥子酶的研究中,把实验室成果应用于菜籽废渣固体发酵制备高蛋白饲料生产中, 技术列入国家级新产品和国家火炬计划项目;从海洋真菌和细菌还发现了具生物活性的萜类, 连烯, 聚酮, 环肽, 类青霉素等新结构化合物;另在植物内生菌研究中,在国际上首次发现了香蕉、水稻等重要作物的内生放线菌类群,发现了抗疫性的内生放线菌并进行新型生物控制剂开发性研究。这些工作获得国家自然科学基金,科技部高技术863计划和省自然科学基金等各级的连续资助。近十年来, 在国内外刊物发表论文60多篇, 合编著作三部“反义RNA与DNA”、”现代生物学”、和“海洋微生物及其代谢产物”。在教学上, 长期讲授本科“微生物遗传学”,“生物工程与制药”、“现代生物技术进展”课程和研究生课程“微生物分子遗传学”、“现代生命科学进展”、“分子克隆原理与技术”等.已培养出一批博士和硕士研究生.。
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【期刊论文】Comparing expression of different forms of human DNA topoisomerase I in Pichia pastoris
周世宁, Guowu Yang a, b, Huiqiong Zhou c, Yongjun Lu a, Yongcheng Lin d, Shining Zhou a, *
Enzyme and Microbial Technology xxx(2003)xxx-xxx,-0001,():
-1年11月30日
In order to rapidly screen new inhibitors of human DNA topoisomerase I in vitro, the enzyme has been successfully overexpressed in the full-length form (hTopoI) and N-terminal 184 amino acids truncated form (hTopoI△581) in Pichia pastoris by the secretory and intracellular styles. The results showed that the recombinant enzyme containing nuclear localization signals obviously decreased its expression level. If the enzyme was fused to α-factor secretory signal or the AKL triplet amino acids (a C-terminal peroxisomal-targeting signal), its output could be highly improved. The recombinant strain SMD-hTopoI△ (out) was best suited to express and secrete the highly active hTopoI△ 581 with part of them glycosylated (the enzyme activity of the supernatant reached 3.4×10 8Ul-1). SMD-hTopoI (out) could produce and secrete the full length form of hTopoI without glycosylation (the enzyme activity of the supernatant reached 4.3×10 7Ul-1). The yield of the recombinant hTopoI reached 11mgl-1, achieving 9.7% of the total protein in the culture supernatant, while that of the recombinant hTopoI△ 581 reached 58mgl-1, achieving 47.3% of the total protein in the supernatant. Both forms of the recombinant enzyme exhibited the same properties as the natural hTopoI.
Human DNA topoisomerase I, Pichia pastoris, Secretory expression
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周世宁, Lixiang Cao, Zhiqi Qiu, Xin Dai, Hongming Tan, Yongcheng Lin and Shining Zhou*
World Journal of Microbiology & Biotechnology 20:501-504, 2004.,-0001,():
-1年11月30日
Two hundred and forty-two actinomycete strains were isolated from the interior of leaves and roots of healthy and wilting banana plants. Most of them were streptomycetes, Streptomyces griseorubiginosus-like strains were the most frequently isolated strains. Community analysis demonstrated increased actinomycete diversity in wilting leaves compared to that in healthy leaves, similar actinomycete communities were found in wilting and healthy roots. Screening of the isolates for antagonistic activity against Fusarium oxysporum f. sp. cubense revealed that the proportion of antagonistic streptomycetes in healthy roots was higher than that in wilting roots (P<0.01), but no difference was found between antagonistic strains isolated from healthy and wilting leaves. The potential biological control of Panama disease of banana by endophytic streptomycetes, especially Streptomyces griseorubiginosus-like strains was discussed.
Endophytic actinomycetes,, Fusarium oxysporum f., sp., cubense,, Musa acuminata,, Panama disease of banana,, streptomycetes
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周世宁, X.L. Tian, L.X. Cao, H.M. Tan, Q.G. Zeng, , Y.Y. Jia, W.Q. Han and S.N. Zhou, *
World Journal of Microbiology & Biotechnology 20:303-309, 2004.,-0001,():
-1年11月30日
The populations of endophytic fungi and actinomycetes from four rice cultivars in the Panyu district (Site 1) and Wushan district (Site 2) in Guangdong province, South China, were studied. The preponderant endophytic fungi and actinomycetes isolated belonged to Fusarium and Streptomyces respectively. The incidence of Streptomycetes griseofuscus ranged from 36.1 to 69% out of all the different rice cultivars from the two sites. It is the commonest population of endophytic actinomycetes, and constituted the greatest part of all the antagonistic communities. The distributions of endophytic fungi and actinomycetes in roots and leaves were different, endophytic fungi from leaves were diverse, some were organ-specific. More diverse endophytic actinomycetes were isolated from roots than from leaves. The endophytic fungi isolated from rice in Site 2 were more diverse than that in Site 1. The diversity of the endophytic actinomycetes, however, was less than that in Site 1. Acid soil in Site 2 is ideal for the growth and colonization of fungi while the alkaline soil in Site 1 is better for the growth and colonization of actinomycetes. The results suggested that differences in the chemical composition of soil could influence the endophytic microbial communities of rice plants. The endophytic fungi and actinomycetes isolated from poor-growing seedlings and susceptible rice cultivars were more abundant than that the disease-resistant counterparts. In the dual culture and activity detection of the metabolites, 41.2% of all the isolated endophytic fungi showed antagonism to rice pathogens. Fifty percent of all the isolated endophytic actinomycetes were antagonistic to those pathogens. The percentage of Streptomyces griseofuscus and hygroscopicus reached 55.4 and 21.4% of all the active actinomycetes.
Endophytic actinomycetes,, endophytic fungi,, rice,, rice blast disease,, Sheath Blight of Rice
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周世宁, L. Cao, Z. Qiu, J. You, H. Tan and S. Zhou
,-0001,():
-1年11月30日
Aims: To isolate endophytic Streptomyces strains from tomato and examine their antimicrobial activity. Methods: Endophytic Streptomyces strains were isolated using surface-sterilization methods and identified by morphological characteristics. Antimicrobial activities were measured by the agar plate sensitivity method. Antifungal activity in vivo was measured by seedling mortality in infested soils. Results: Twenty-one per cent of endophytic streptomycete isolates produced antibacterial metabolites and 41% produced antifungal metabolites in S medium. Sixty-five per cent of the most frequently isolated strains inhibited the growth of Rhizoctonia solani by the antibiosis assay but only 32% produced metabolites active against R. solani in S medium. Growth promotion and enhanced disease resistance of seedlings inoculated with Streptomyces sp. strain S30 were observed in tomato but not in cucumber seedlings. Conclusions: Endophytic Streptomyces spp. strains were successfully isolated using stringent methods and strain S30 promoted growth and enhanced resistance to R. solani in tomato seedlings. Significance and Impact of the Study: Endophytic streptomycetes showing antifungal activity in vitro and in vivo may indicate the potential for their use as biocontrol agents particularly of R. solani disease of tomato seedlings.
biocontrol agents,, endophytic actinomycetes,, Rhizoctonia damping-off,, Streptomyces,, tomato.,
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