马正强
小麦基因组学和生物技术育种
个性化签名
- 姓名:马正强
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
农艺学
- 研究兴趣:小麦基因组学和生物技术育种
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主页访问
2019
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0
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成果阅读
371
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成果数
10
马正强, Z.-Q. Ma, B. S. Gill, M. E. Sorrells, and S. D. Tanksley
Their Appl Genet (1993) 85: 750-754,-0001,():
-1年11月30日
Summary. Restriction fragment length polymorphism (RFLP) markers linked to genes controlling Hessian fly resistance from Triticum tauschii (Coss.) SchmaI. were identified for two wheat (Triticum aestivum L.) germ plasm lines KS89WGRC3 (C3) and KS89WORC6 (C6). Forty-six clones wlth loci on chromosomes of homoeologous group 3 and 28 clones on those of group 6 were surveyed for polymorphisms. Eleven and 12 clones detected "E tauschii loci in the two lines, res-pectively. Analysis of F2 progenies indicated that the Hessian fly resistance gene H23 identified in C3 is linked to XksuH4 (6.9cM) and XksuG48(A) (15.6cM) located on 6D. The resistance gene H24 in C6 is linked to XcnlBCD45I (5.9eM), XcnlCD0482 (5.9cM) and XksuG48(B) (12.9cM), located on 3DL.
Wheat (, Triticum aesticum), -Hessian fly-Resistance genes-RFLP markers
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【期刊论文】RFLP markers linked to powdery mildew resistance genes Pml, Pro2, Pro3, and Pro4 in wheat1
马正强, Z.Q. MA, M.E. SORRELLS, AND S.D. TANKSLEY
GENOME. VOL. 37, 1994,-0001,():
-1年11月30日
Near-isogenic lines (NILs) and their recurrent parent Chancellor (Co) were used to identify restriction fragment length polymorphic markers linked to powdery mildew (Blumeria graminis (DC.) E.O. Speer f.sp. tritici) resistance genes Pm], Pro2, Pro3, and Pro4 in wheat (Triticum aestivum L. em. Theli). By mapping these poIymorphic markers in F: progenies from crosses of the NILs with C, it was found that Pro1 cosegregated with a polymovphic locus detected by DNA probe CDO347; Pro2 was linked to a locus detected by probe B CD[871 with a distance of 35 cM; Pm3b was linked to a locus detected by probe BCD1434 with a distance of 1.3 cM; Pm4a cosegregated with Xbcd1231-2A(2) and Xcdod78-2A, and was closeIy flanked by Xbcd1231-2A(t) and Xbcd292-2A both wiEh a distance of 1.5 cM. Aneuploid mapping of these markers indicated that locus Xcdo347-TA is on 7AL, Xbcd187]-5D on 5DS, Xbcd1434-1A on IAS, and loci Xbcd292-2A and Xcdo678-2A are on 2AL. The same polymorphic fragments detected in the Pm3b NIL by Xbcd1434-1A were found in Pm2a NIL. using several enzyme digestions.
RFLP markers,, Pint,, Pro2,, Pm3,, Pro4,, Blumeria graminis (, DC., ), E., p., Speer f., sp., tritici (, Erysiphe graminis f., sp., tritici), ,, wheat (, Triticum aestivum L., era., Thell), ,, gone tagging.,
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【期刊论文】PCR-based markers for the powdery mildew resistance gene Pm4a in wheat
马正强, Z.-Q. Ma
Theor Appl Genet (2004) 109: 140-145,-0001,():
-1年11月30日
Gene tagging is the basis of marker assisted selection and map based cloning. To develop PCR based markers for Pm4a, a dominant powdery mildew resistance gene of wheat, we surveyed 46 group 2 microsatellite markers between Pm4a near isogenic line (NIL) CI 14124 and the recurrent parent Chancellor (Cc). One of the markers, gwm356, detected polymorphism and was used for genotyping an F2 population of 85 plants derived from CI 14124 x Cc. Linkage mapping indicated that Xgwm356 was linked to Pm4a at a distance of 4.8cM. To identify more PCR based markers for Pm4a, we also converted the restriction fragment length polymorphism marker BCD1231 linked to it into a sequence tagged site (STS) marker. The STS primer designed based on the end sequences of B CD 1231 amplified an approximately 1.6kb monomorphic band in both parents. Following digestion of the products with the four cutter enzymes HaeⅢ and Mspl, it was discovered that the band from CI 14124 consisted of at least two products, one of which had a digestion pattern different from the band from Cc. In the F2 population, the cleaved polymorphism revealed by the STS marker between the parents co segregated with powdery mildew resistance. To design Pm4a specific PCR markers, the 1.6 kb band from Cc and the fragment associated with Pm4a in CI 14124 were sequenced and compared. Based on these sequences a new PCR marker was identified, which detected a 470 bp product only in the Pm4a containing lines. These PCR based markers provide a cost saving option for marker assisted selection of Pm4a.
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【期刊论文】Mapping of Quantitative Trait Loci for Fusarium Head Blight Resistance in Barley
马正强, Zhengqiang Ma, Brian J.Steffenson, Louis K. Prom, Nora L. V. Lapitan; Ma, Z, Steffenson, B. L, Prom, L. K., and Lapitan, N. L. V.
Genetics and Resistance Vol. 90, No.10, 2000,-0001,():
-1年11月30日
Fusatium head blight (FHB) is a devastating disease that causes significant reductions in yield and quality in wheat and barley. Barley grains infected with deoxynivalenol (DON), a vomitoxth produced by Fusarium graminearum, are rejected for malting and brewing. Among six-rowed barley cultivars tested thus far, only cv. Chevron exhibited resistance. This study was conducted to map genes and to identify DNA markers for marker-assisted breeding for FHB resistance in cv. Chevron with restriction fragment length polymorphism (RFLP) markers. A doubled haploid (DH) population was created from a cross between cv. Chevron and susceptible cv. Stander. Seven field ex-periments were conducted in four different locations in 2 years. A RFLP map containing 211 loci and covering over 1,000 centimorgaas (cM) of the genome was used to map quantitative trait loci (QTL) associated with relatively low FHB severity and DON concentration. Morphological traits differing between the parents were also measured: heading date, plant height, spike angle, number of nodes per cm of rachis in the spike, and kernel plumpness. Many of the QTL for FHB and DON coincided with QTLs for these morphological traits. The "fix-QTL" algorithm in Mapmaker QTL was used to remove the part of the variance for FHB resistance that may be explained by heading date or plant height. Results from this study suggest that QTLs with major effects for FHB resistance probably do not exist in cv. Chevron. Three QTL intervals, Xcmwg706-Xbcd441 on chromosome IH, Xbcd307b-Xcdo684b on chromosome 2H, and Xcdo959b-Xabg472 on chromosome 41-1, that are not associated with late heading or height may be useful for marker-assisted selection.
Hordeum vulgate,, scab.,
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马正强, Z.-Q. Ma
Genome. 41: 303-306 (1998),-0001,():
-1年11月30日
Restriction fragment length polymorphism (RFLP) markers were used to map male fertility restor- ing gene that was transferred from chromosome 6U of Aegilops umbeIIulata Zhuk. to wheat. Segments of ehro-mosome 6U bearing the gene that restore fertility to T. tirnopheevi Zhuk. male sterile'cytoplasm were identified in all four translocation lines by two probes, BCD2t and BCD342. Lines 040-5, 06 l-I and 0614 are T6BL.6BS-6U translocations, while Iine 2114 is a T6AL.6AS-6U translocation, Line 211~ has a much larger 6U chromo-somal segment and lower frequency of transmission of male gametes with the alien segment than the other three lines The restoring gene carried by the 6U segment in 2114 showed high expressivity and complete penetrance. This restoring gene is designated Rf6. A homoeologous chromosome recombination mechanism is discussed for the alien gene transfer
Rf6
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【期刊论文】Genetic mapping of Russian wheat aphid resistance genes Dn2 and Dn4 in wheat
马正强, Z.-Q. Ma, A. Saidi, J.S. Quick, and N.L.V. Lapitan
Gnome, 39: 123-130 (1996). Printed in Canada/Imprime au Canada,-0001,():
-1年11月30日
To obtain markers for marker-assisted breeding of Russian wheat aphid resistance in wheat (Triticum aestivum L.), resistance genes Dn2 and Dn4 were mapped with restriction fragment length polymorphism (RFLP) markers, using populations derived from PI 62660 x 'Carson' and Pl 372129 x 'Yuma'. P1262660 and P1372129 are the donor parents of Dn2 and Dn4, respectively. A locus detected by marker KsuA I was linked to DI72 at a distance of 9.8 cM on the long arm of chromosome 7D, and a locus detected by marker ABC 156 was 11.6cM away from Dn4 on the short arm of chromosome ID.
Russian wheat aphid., RFLP markers,, Triticum aestivum
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马正强, Z.Q. Ma, M. Roder, and M.E. Sorrells
Published in Crop Sci. 35: 1137-1143 (1995),-0001,():
-1年11月30日
Microsatetlites have emerged as an important source of genetic markers for eukaryotic genomes. In this report, two wheat (Triticum aestivurn L.) genomic libraries were screened for several di-. tri-. and tetra-nucleotide tandem repeats. Clones containing (AC)n, (AG)n. (TCT)n. and (TTG), repeats were isolated and sequenced. On average, there was one (AC)n microsatellite every 292 kbp and one (AG) microsatellite every 212 kbp. The trinucleotide tatdern repeats (TCT) and ('I'rG) were about 10 times less common than the two dinucleotide tandem repeats tested and tetranueleotide tandem repeats were rare. Many of the microsate[lites had more than 10 repeats. The maximum repeat number found for (AC), was 36 and for (TCT)n was more than 50. The prevailing category of (AG) microsatel|ites from (AG), isolates was perfect repeats. About half of the (AC), microsatellites were compound repeats, while most of the (TCT). microsatellites were imperfect repeats. In a small sample. (TTG), microsatellites consisted mainly of compound repeats. The most frequently associated repeats were (AC)n with (AG)n. (TCT), with (TCC),. and (TTG), with (TGG)n. Among 32 pairs of microsatellite primers surveyed, seven.produced polymorphic products in the expected size range and theze loci were mapped using a hexaploid wheat mapping population or aneuploid stocks.
wheat., Triticum aestivum L., ,, microsatellites,, polymorphism,, sequence characteristics.,
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马正强, Z.-Q, Ma and Mark E. Sorrells*
Published in Crop Sci. 35: 1137-1143 (1995),-0001,():
-1年11月30日
Fertility restoration for Triticum timopheevi Zhuk, cytoplasmic male steriligy in wheat (Triticum aestivum L.) is controlled by both major fertility restorer (Rf) grnes and modifier genes together with environ-ment efiects. Therefore, the breeding of efficient and stable male-fertility restorer lines (R-lines) should be facilitated by marker-assisted selection. This study was conducted to ldentify restriction fragment length polymorphism (RFLP) markers for the Rf genes, Rf1 and Rf4 in restorer line R113, and Rf3 and a Rf gene on 5D in cultivar Primepi, Polymorphic markers were mapped in testcross F1 populations and their association with fertility restoration was analyzed by analysis of variance. The results Indicated that Rf4 was linked to Xksug48 located on 6BS in R113, Rf3 was linked to Xbed249 and Xcdo442 on 1BS, and Xcdo786 may be associated with the 5D Rf gene in Primepi. In addition, in R113 two other chromosome regions with fertility restoration-associated loci were detected by CD0786 and H1 on 5AL and CD-O1189 on 7BS. A locus on 5DS, detected by BCD1871, showed significant epistatic effect on the expression of Rf genes. InPrimepi, a locus on 5AL, detected by Xbcd183 and Xbcd876, had effects similar to Xcdo786. Two loci in Qu Xian Early A', identified by Xbcd871 and Xwg1026, may be associated with genes controlling ease of restoration in this male-sterile line (A-line). Except for Xbcd1871 and Xwg1026, the effects of other significant RFLP loci were additive in both populations.
RFLP,, restriction fragment length polymorphism, QTL,, quantitative trait loci, Rf,, male fertility restorer, A-line,, male-sterile line, R-line,, restorer line, cM,, centimorgan, Nor,, rRNA gene, EDTA,, ethylene-diaminetetraacctic acid, SDS,, sodium dodecyl sulfate, SSC,, standard saline citrate.,
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【期刊论文】A comparison of amplified and restriction fragment length polymorphism in wheat
马正强, Zheng-Qiang Ma and Nora L.V. Lapitan*
Cereal Research Communications Vol. 26 No.1 1998 7~13,-0001,():
-1年11月30日
The amplified fragment length polymorphism (AFLP) technique was assessed for its effectiveness as a marker system in wheat, a hexaploid specles with a large genome (1.6x1010 bp per haploid). The degree of polymorphism detected by AFLP among 11 pure lines of wheat was determined and compared with the polymorphism detected by RFLP. Cost comparison between AFLP and RFLP techniques was also made. The appropriate amount of wheat genomicDNA as template in pre-amplification was first determined. All 20 AFLP primer pairs tested resulted in amplification of bands which were polymorphic between all pairwise genotype comparisons. On average, 106 products per genotype were amplified, and for each primer pair used, more than 25 polymorphic fragments detected polymorphism among the 11 genotypes. In comparison, only 61% of 18 RFLP probes tested detected polymorphism among 10 of the 11 lines and each polymorphic probe detected an average of only three bands showing polymorphism between genotype pairs. The AFLP technique is more efficient but less expensive and requires less labor than the R.P technique in wheat.
Wheat,, AFLP,, RFLP,, polymorphism
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