张绍铃
果树栽培与生殖生理、种质创新等
个性化签名
- 姓名:张绍铃
- 目前身份:
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学术头衔:
博士生导师
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学科领域:
园艺学
- 研究兴趣:果树栽培与生殖生理、种质创新等
张绍铃,男,1961年12月出生,中共党员,博士后,南京农业大学果树研究所副所长;南京农业大学园艺学院教授、2000年人选博士生导师;南京农业大学园艺学一级学科博士点的《园艺作物生理 》研究方向的学术带头人。
社会兼职有:中国园艺学会梨分会副理事长,江苏省发育生物学会副秘书长;《果树学报》编委,江苏省新世纪学科带头人“333工程”第二层次(国内知名)培育对象入选者。
主要学术成绩:20余年来,主要从事了果树栽培与生殖生理、种质创新等的科研、教学工作,在梨树生殖生理研究领域取得了一些原创性研究成果,多年的研究积累阐明了一些梨自交不亲和及亲和性机理的学术理论和创建了一些研究方法。如在国际上,率先提出了梨自交不亲和性强度取决于自交不亲和基因(S基因)所表达的S糖蛋白量;确定了梨品种S1~S7的7个S基因蛋白产物(S-RNase),率先分离纯化出了雌蕊各个S基因所表达的S-RNase,阐明了的雌蕊S基因时空表达特性及花柱S-RNase抑制花粉管生长的模型的理论;创建的花柱半离体培养法应用于梨品种自交不亲和性强度的分类及鉴定品种的S基因型;率先将细胞生物学的理论与方法应用于果树自交不亲和性机理的研究,促进学科交叉研究领域的发展;开展了梨雌蕊S基因克隆研究,克隆了雌蕊S28-RNase基因等。此外,开展了自交亲和性种质的创新和自交亲和性品种选育的应用研究,已创新了10个自交亲和性品系,为实现1个果园栽植1个品种,不用配置授粉品种奠定了基础。
累计获得科技成果奖8项,其中省部级以上科技成果奖4项,如,教育部提名国家自然科学奖二等奖1项,《梨配子体型自交不亲和性机制的研究》,2002年,排名第一);申请国家发明专利2件;出版著作、教材9部;发表了近50篇论文,主要研究论文在HortScience、 Sex. Plant Reprod.、J. Japan Soc. Hort. Sci.、植物学报等国内外学术刊物上发表。其中SCI收录7篇,ISTP收录1篇。 累计主持或参加完成的科研项目有12项,目前主持的在研项目有6项,其中国家自然科学基金项目2项,高校博士学科点专项科研基金项目1项,“863”项目1项(第二主持人),科技部农业科技成果转化项目1项,江苏省高技术项目1项等6项。在人才培养方面,已培养的博士2名,留学生1名,硕士4名,目前指导的在校博士后1名,博士生13名,硕士生14名。
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成果数
7
张绍铃, Shao- Ling Zhang and Shin Hiratsuka
(J. Japan. Soc. Hort. Sci.) 68(5): 911-918. 1999,-0001,():
-1年11月30日
When excised styles of the Japanese pear were self- pollinated and cultured on an agar medium,the percentage of styles with protruding pollen tubes onto the medium from cut ends variedsignificantly among different Japanese pear cultivars. Based on these data, the cultivars wereclassified into the following groups; strongly, intermediately, and weakly self-incompatible (SI),and their levels of S- protein in the style were compared. The levels of S-proteins which were separated by isoelectricfocusing polyacrylamide gelelectrophoresis (IEF-PAGE) and quantified by an image analyzer differed considerably amongeach of S-proteins (S3>S1>S5>S4>S6>S2>S7), even when they were produced from the sameS - allele. The sum of two allelic S-proteins correlated positively with the strength of SI in the cultivars(r=0.876). The cultivars which were strongly, intermediately, and weakly SI contained 1.51, 1.23,and 0.67ng of S-protein per μg of buffer extractable protein, respectively. But the level ofindividual S-proteins was not directly related to the strength of SI in the cultivars. The totalamount of S- proteins was mainly due to the combination of 2 S- proteins in the cultivar, i. e. thecultivars with S1-, S2- and /or Ss-allele generally contained high level of S-proteins. Thedistribution pattern of the S- proteins in the style was unrelated to the SI strength of the cultivar. Thus, each Japanese pear cultivar may possess different strengths of SI system which may bethe function of the total S- proteins in the style.
Japanese pear cultiver,, pollen tube growth,, strength of self-incompatibility,, stylar S-protein level.,
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张绍铃, Shin Hiratsuka*, Shao-Ling Zhang
Scientia Horticulturae 95(2002)309-318,-0001,():
-1年11月30日
Fruit set after self-pollination varied from 0.6 to 15.2%and the growth rate of self pollen-tubes in thestyle also varied from 0 to 39% among the Japanese pear cultivars (Pyrus serotina Rehd.); the formerdata were calculated using the results reported by several workers at different locations during severalyears, and the latter were our experimental results using a stylar culture technique in vitro. Cultivardifferences in fruit set matched well with differences in the tube growth (r=0.852**, n=12) (**; significant at 1% level by t-test). Concentration of the sum of two S-RNases in the style was cultivardependent,and it showed a negative relation to fruit set (r=-0.764*, n=7) (*; significant at5%level)or pollen-tube growth (r=-0.888**, n=18). The correlation coefficients between the pollen-tubegrowth and S1-, S2-, S3-, S4-, or S5-RNase concentration were; -0.714(n=5), -0.242(n=6), -0.560(n=4), -0.701*(n=9), or -0.948(n=4), respectively. Cultivar differences in theexpression of self-incompatibility may, thus, be present in the Japanese pear and regulated, at leastin part, by the concentration of S-RNase differentially produced in the style of each cultivar.
Fruit set, Japanese pear, Pollen-tube growth, Self-incompatibility expression, S-RNase
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张绍铃, Shao-Ling Zhanga, Shin Hiratsukab, *
Scientia Horticulturae 104(2005)169-178,-0001,():
-1年11月30日
The Japanese pear 'Osa-Nijisseiki' (S2S4SM) (SM=stylar-part mutant) is a self-compatible budmutant that originated fromself-incompatible 'Nijisseiki' (S2S4).The S4-allele of the pear is deleted andit shows unilateral incompatibility to cultivar with an S2S4 genotype. However, when pollen-tubegrowth was compared between cross-compatible ['Osa-Nijisseiki'_'Okusankichi' (S5S7)], unilateralcompatible['Osa-Nijisseiki'_'Kikusui'(S2S4)], self-compatible ('Osa-Nijisseiki'_'Osa-Nijisseiki'),and unilateral-incompatible pollination ('Kikusui'_'Osa-Nijisseiki'), pollen-tube growthclearly showed the following order: cross-compatible > unilateral-compatible > self-compatible >unilateral-incompatible. This indicates that the 'Osa-Nijisseiki' style produces specific inhibitor(s) notonly to S2- andS4-pollen but also "self-pollen", because the phenotype of S4SM-pollen is the sameas S4-pollen. Stylar protein analysis demonstrated that 'Osa-Nijisseiki' produces S2-RNase (RNase associatedwith S2-allele) together with a small amount of S4-RNase. The purified S4-RNase possessedalmost the same inhibitory action on the growth of S4-pollen-tubes in vitro at 1 mg ml_1 as that fromoriginal 'Nijisseiki'. These results suggest that the depressed growth of unilateral-compatible and selfpollen-tubes in 'Osa-Nijisseiki' is due to this biologically active S4-RNase. Growth of self-pollen-tubesmay also be depressed by inhibitor(s) specific to "self-pollen" unrelated to S-alleles.
Cross-pollination, Difference in pollen-tube growth, Japanese pear, Self-pollination, S-RNase
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张绍铃, Shin Hiratsuka*, Shao-Ling Zhang
Scientia Horticulturae 95(2002)309-318,-0001,():
-1年11月30日
Fruit set after self-pollination varied from 0.6 to 15.2%and the growth rate of self pollen-tubes in the style also varied from 0 to 39% among the Japanese pear cultivars (Pyrus serotina Rehd.); the former data were calculated using the results reported by several workers at different locations during several years, and the latter were our experimental results using a stylar culture technique in vitro. Cultivar differences in fruit set matched well with differences in the tube growth (r=0:852**, n=12) (**;significant at 1% level by t-test). Concentration of the sum of two S-RNases in the style was cultivardependent,and it showed a negative relation to fruit set (r=-0:764*, n=7) (*; significant at5%level) or pollen-tube growth (r=-0:888**, n=18). The correlation coefficients between the pollen-tube growth and S1-, S2-, S3-, S4-, or S5-RNase concentration were;-0.714 (n=5),-0.242 (n=6),-0.560 (n=4),-0.701* (n=9), or-0.948 (n=4), respectively. Cultivar differences in the expression of self-incompatibility may, thus, be present in the Japanese pear and regulated, at least in part, by the concentration of S-RNase differentially produced in the style of each cultivar.
Japanese pear, Pollen-tube growth, Self-incompatibility expression, S-RNase
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【期刊论文】Cultivar Differences in the Expression of Self-Incompatibility in Japanese Pears
张绍铃, S. Hiratsuka, S L. Zhang
Poc. IS on Asian Pears Eds. S. Iwahori et al. Acta Hort, 587, ISHS 2002,-0001,():
-1年11月30日
Data on cultivar differences in fruit set after self-pollination reported by many researchers were summarized. They varied from 0.6 to 15.2%, and our result on the growth rate of self pollen-tubes in the styles also varied from 0 to 47% among the cultivars. Cultivar differences in fruit set matched the differences in the tube growth (r=0.852★★), indicating that each Japanese pear cultivar expresses different degrees of self-incmpatibility (SI). S-RNase (RNase associated with SI allele) concentrations in the style, which were quantified by scanning the protein bands on electrophoresed gel, were cultivar-dependent. They showed negative relations to fruit, set (r=-0.764) and the growth rate of self pollen-tubes (r=0.88★★), respectively. Purified S-RNases caused selective inhibition in vitro on the growth of self pollen-tubes (pollen-tubes from the same cultivar from which the S-RNases were prepared), and the inhibition was dose-dependent. S-RNases prepared from the cultivars with different degrees of Sl expression exhibited almost the same inhibitory action at the same concentration. From these results, we conclude that cultivar differences in the expression of SI exist, and they may be regulated by tile concentration of S-RNase differentially produced in the style of each cultivar of the Japanese pear.
pyrus pyrifolia Nakai,, fruit set,, pollen-tube growth,, S-RNasc,, sclf-thinning cultivar
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张绍铃, Shao-liang Zhang and Shin Hiratsuka
HORTSCIENCE, VOL. 35 (5), AUGUST 2000,-0001,():
-1年11月30日
Cultivars of the Japanese pear [Pyrus pyrifolia (Burm.) Nakai] have variable degrees of self-incompatibility (SI) and can be classified into at leas three groups: strong, intermediate, or weak S; as shown by the extent of self-pollen tube growth in the style, and the percentage of fruit set following self-pollination. Following self-pollination, the elongation of pollen tubes in the detached styles of 'Kosui' and 'Kikusui' beame increasingly suppressed form 4 days before antheis (-4 DAA) to 2 days after anthesis (2 DAA). Tube growth of 'Kosui' was more suppressed than that of 'Kikusui' during this period, In 'Osa-Nijisseiki', however, the rrate of tube growth did not vary with stage of stylar development, form-8to 2 DAA. Pollen tubes elongated mutch much better after cross-pollination than after self-pollination at all stages testd, and the extent of the elongation increased as the styles matured. The concentration of total S-protein (sum oftwo S-[rpteoms [er niffer-soluble protein) increased with stylar development, but the rate of increase varied with the cultivar. The rate was significantly greatr in the strogly self-incompatible 'Kosui' than in the moderately self-incompatible 'Kikusui', and was slowest in the weakly selfincompatible 'Osa-Nijisseiki' at all developmental stages. During stylar maturation, the concentration of S4protein, which is common in all cultivars, was highest in 'Kosui', followed by 'Kikusi' and 'Osa-Nijisseiki', Thus, the cultivar differences in SI expression in the Japanese pear are deterimed about-4 DAA and apperar to be regulated, in part, by the concentraion of S-roteins produced in the style.
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【期刊论文】花柱培养法+によるニホンナシの自家および交配不和合反応の品种间差の解析
张绍铃, 张绍铃·平塚伸
园学雑. (J. Japan. Soc. Hort. Sci.) 68 (2): 373-383. 1999.,-0001,():
-1年11月30日
Varietal differences of pollen tube growth among self. cross incompatible and cross compatible pollinations were investigated by semi vitro assay using detached styles in Japanese pcar (Pyrus serotina Rehd. var. culla Rehd) cultlvars Among the 52 self-compaIiblc and incomoatible cultivars, the pollen tubes grew in considerably different ways The percentages of styles with protruding pol]en tubes from the basal cut end ranged from 0 to 47. Pollen tubes of 'Shblsetsu', Nibonyamanashi, se]f compatible 'Osa-Nijisseiki', and sell compatible strains obtained from 'Osa-Nijisseiki' grew wcg, and those of 'Okusankitsu' 'Yasato', 'Kikusui', 'Ni isseiki', and 'Kisui' did intermediately, whereas pollen tubes of "Shinsui', 'Choiuro", 'Khnizukawasc' 'Hohsui', "Kohsui'. and 'Kohzo' grew poorly Similar tendencies were observed when the number of pollen tubes protruding from the basal cut end among cultivars was counted.Generally, pollen tubes of cultivars derived from 'Nijisseiki' grew better than dgl those of 'Kohsui and 'Shinsui', indicating that the tate of pollen tube elongation of cultivars is a heritable character In cross incompatible and compatible pollnation combinations, the pollen tubes grew quite differently, depending on the parental combinationations. The percentages of styles with protruding pollen tubes from the cut end ranged from 0 to 40 in cross incompatible combinations, and from 26 1o 97 in cross compatible ones By using excised styles shortened to 1/2 or 1/3 of the original length, the percentage of styles with protruding pollen tubes from the cut end improved. The growth of self and cross incompatible pollen tubes became much better so that the promotion patterns were classified as fullows: 1) tube elongation becomes better as the style is shortened: 2) tube elongation becomes better but there arc no differences between 1/2 and 1/3 reduction in style length; 3) pollen tube elongation becomes belier only in the I/3 style length, and 4) no change was found These differences indicate that the inhibition of pollen tube growth occurs in different paris of 1he style among respective cultivars.
Japanese pear,, pollen tube growth,, self-incompatibility,, style culture,, varietal difference.,
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