何治柯
荧光及化学发光分析、微流在线分析及微流控芯片化学发光检测技术、高通量传感器、量子点合成及分析应用等
个性化签名
- 姓名:何治柯
- 目前身份:
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学术头衔:
博士生导师
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学科领域:
分析化学
- 研究兴趣:荧光及化学发光分析、微流在线分析及微流控芯片化学发光检测技术、高通量传感器、量子点合成及分析应用等
何治柯,博士,男,武汉大学化学与分子科学学院教授、博士生导师,德国洪堡学者。95年武汉大学化学系博士毕业(导师:曾云鹗)并留校工作,96年破格晋升副教授,99年破格晋升教授,2000年被批准为博士生导师。98-99,荣获德国洪堡基金资助,赴德国Halle大学生物技术学院从事生化分析研究,2000-2001及2002-2003赴香港理工大学从事高通量生物传感器研究。研究方向包括:荧光及化学发光分析、微流在线分析及微流控芯片化学发光检测技术、高通量传感器、量子点合成及分析应用等。在国内外重要学术刊物上发表学术论文80余篇(SCI收录62篇)。部分研究成果获湖北省自然科学奖二等奖(排名第二)。申请发明专利6项,其中一项为美国专利。鉴定成果3项。
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308
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成果数
10
何治柯, He-You Han
Fresenius J Anal Chem (1999) 364: 782-785,-0001,():
-1年11月30日
A direct chemiluminescence method for the determination of nucleic acids has been developed based on the enhancement of nucleic acids on the chemiluminescence light emission of the reaction between Ru(phen)3 2+(phen=1,10-phenanthroline) and Ce(IV). Under the optimum conditions, the calibration graphs are linear in the range of 5.0
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何治柯, He Zhike *, Gao Hua, Yuan Liangjie, Lu Shaofang, Meng Hui, Li Xiaoyan, Zeng Yun'e
Talanta 47(1998)301-304,-0001,():
-1年11月30日
enhancement of citric acid on the chemiluminescence light emission of tris-(2,2%-bipyridine) ruthenium (II). In the presence of tris-(2,2%-bipyridine) ruthenium (II), upon the addition of Ce (IV), resulted in intense light emission. The emission intensity is greatly enhanced by the presence of citric acid. The linear range and detection limit of citric acid are 3.0×10-8~6.0×10-6mol l-1 and 3.0×10-8mol l-1, respectively. The precision of the proposed method is determined by analyzing 11 samples containing 1.0×10-7mol l-1 citric acid. The relative standard deviation is 3.0%. The enhanced mechanism of citric acid was studied. The method was evaluated by carrying out an nterference study with common ions and compounds, by a recovery study and by analysis of human urine and orange juice. A satisfactory result was obtained.
Chemiluminescence, Citric acid, Tris-(, 2,, 2%, -bipyridine), ruthenium (, II),
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【期刊论文】Simultaneous Determination of Oxalic and Tartaric Acid With Chemiluminescence Detection
何治柯, Zhike He*, Hua Gao, Liangjie Yuan, Qingyao Luo and Yun'e Zeng
Analyst, November 1997, Vol. 122 (1343-1345),-0001,():
-1年11月30日
A time-resolved chemiluminescence method for the determination of oxalic and tartaric acid was developed. The method is based on the kinetic distinction of the chemiluminescent reaction of Ru(bipy)3 2+and oxalic and tartaric acid with CeIV. The oxalic acid system gives the highest chemiluminescence intensity at 2s, whereas the tartaric acid system gives its most intense chemiluminescence emission at 50s. Oxalic acid (1.4
Simultaneous determination, oxalic acid, tartaric acid, chemiluminescence detection
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【期刊论文】A NEW CHEMILUMINESCENCE REAGENT FOR THE DETERMINATION OF RUTHENIUM
何治柯, ZHIKE HE, QINGYAO LUO, HUImN MA, XIMAO YU and YUN'E ZENG
Talanta, Vol. 41, No.5, pp. 707-710, 1994,-0001,():
-1年11月30日
Snmmary-A new reagent proflavine-N, N, N', N'-tetraacefic acid (PTA) synthesized in our laboratory has been found useful as a chemiluminescence reagent for the determination of ruthenium (Ru) in pH 2.5 sulfuric acid solution containing acetone, it can be oxidized catalytically by hydrogen peroxide. With Ru (III) as catalyst, it emits light selectively. The linear range is between 2.0
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【期刊论文】Functionalized CdSe quantum dots as selective silver ion chemodosimeter
何治柯, Jian-Gong Liang, Xin-Ping Ai, Zhi-Ke He* and Dai-Wen Pang
Analyst, 2004, 129, 619-622,-0001,():
-1年11月30日
CdSe quantum dots (QDs) have been prepared and modified with mercaptoacetic acid. They are water-soluble and biocompatible. To improve their fluorescence intensity and stability in water solution, bovine serum albumin (BSA) was absorbed onto their surface. Based on the quench of fluorescence signals of the functionalized CdSe QDs in the 543nm wavelength and enhancement of them in the 570-700 nm wavelength range by Ag (I) ions at pH 5.0, a simple, rapid and specific method for Ag (I) determination was proposed. In comparison with single organic fluorophores, these nanoparticles are brighter, more stable against photobleaching, and do not suffer from blinking. Under the optimum conditions, the response is linearly proportional to the concentration of Ag (I) between 4.0
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【期刊论文】Chemiluminescence determination of barbituric acid using Ru (phen)32+-Ce(IV) system
何治柯, Juan Xi, Xinping Ai, Zhike He *
Talanta 59(2003)1045-1051,-0001,():
-1年11月30日
A chemiluminescence (CL) method for the determination of barbituric acid (BA) was proposed, which is based on the enhancement of BA to the CL intensity of Tris /(1,10-phenanthroline)ruthenium (II) (Ru(phen)32+-cerium (IV) (Ce(IV)) system. The concentration of BA is proportional to the CL intensity in the range of 5.0
Chemiluminescence, Barbituric acid, Tris-(, 1,, 10-phenanthroline), ruthenium (, II),
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【期刊论文】Determination of DNA by Rayleigh light scattering enhancement of molecular "light switches"
何治柯, Fang Chen, a Jianping Huang, ab Xinping Aia and Zhike He*a
Analyst, 2003, 128, 1462-1466,-0001,():
-1年11月30日
Base on the enhancement of Rayleigh light scattering signals of molecular "light switches" by DNA under acidic condition, a sensitive and convenient method for DNA determination was proposed. The experiments indicated that, under optimum conditions, good linear relationships were obtained between the Rayleigh light scattering intensity and the concentration of nucleic acids. The detect limits of calf thymus DNA (ctDNA) were 13.0ng ml-1, 4.2ng ml-1, 51.5ng ml-1 and 3.0ng ml-1 with four "light switches", respectively. Plasmid DNA extracted from Bacillus subtilis were determined by the proposed method with satisfactory results, and the recovery rates of calf thymus DNA were in the range of 94.6-110.7%.
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何治柯, Lian-Sheng Ling a, b, ∗, Zhi-Ke Hea, Gong-Wu Songa, Yun E. Zenga, Chen Wang b, Chun-Li Bai b, Xiang-Dong Chenc, Ping Shen c
Analytica Chimica Acta 436(2001)207-214,-0001,():
-1年11月30日
A novel fluorimetric method is developed for selective determination of DNA with the molecular "light switch" complex of Ru(phen)2(dppx)2+. The maximal fluorescence intensity is produced in the pH range of 9.3-11.5, with the maximum excitation and emission wavelength of 464 and 594nm, respectively. Under the optimum conditions, the fluorescence intensity was in proportion to the concentration of DNA. The linear range for calfthymus DNA, salmon sperm DNA and herring sperm DNA is 0-0.2g/ml. The limits of detection for calfthymus DNA, salmon sperm DNA and herring sperm DNA are 0.3, 0.24 and 0.74ng/ml, respectively. And the proposed method was successfully used to determine DNA contents in real sample, which was dealt with RNase before the determination. The mechanism of the fluorescence sensitization is also studied. We also found that Ru(phen)2(dppx)2+ preferalls intercalates into AT base pairs to GC base pairs.
Ru(, phen), 2(, dppx), 2+, , DNA, Fluorimetry, Molecular ", light switch",
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何治柯, Z.K. He, B. Fuhrmann, U. Spohn *
Analytica Chimica Acta 407(2000)203-212,-0001,():
-1年11月30日
Coulometric flow titrations were combined with chemiluminescent detection of the equivalence points. The flow channel system consists of a miniaturized flow electrolytic cell, a knotted type tubular reactor and a capillary wall-jet chemiluminescence detector with an outer tube for the addition of luminol reagent solution. The chemiluminescence detector can be used to determine bromine in the range between 0.1mM and 1mM opening up a sensitive way to follow flow titrations with linear concentration gradients of bromine. Very low concentrations of arsenite can be titrated in a sample flow channel system with electrogenerated bromine. The chemiluminescence detection was compared with the amperometric monitoring of the titration curves. To perform such gradient controlled flow titrations without calibration, it was necessary to minimize the so-called tailing. At gradient generation times 120s, arsenite can be titrated almost without calibration in the range between 0.05 and 100mM. The accessible determination range is determined by the maximum electrolysis current and the sample flow rate. Added micromolar concentrations of arsenite were accurately determined in river water and phosphate solutions.
Coulometric flow titration, Chemiluminescence detection, Arsenite, Calibrationless determination
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【期刊论文】Calibrationless Determination of Creatinine and Ammonia by Coulometric Flow Titration
何治柯, Z. K. He, B. Fuhrmann, and U. Spohn
Analytical Biochemistry 283, 166-174 (2000),-0001,():
-1年11月30日
A precise and sensitive working micro-ow titration procedure was developed to determine creatinine and ammonia in urine samples. This procedure is based on enzymatic conversion of creatinine, gas diffusional membrane separation of the released ammonia into an acid acceptor stream, and coulometric titration of ammonia with hypobromite. The hypobromite is formed after the electrogeneration of bromine in an electrolyte containing 1.0M NaBr and 0.1M sodium borate adjusted to pH 8.5. The electrolysis current follows a triangle-programmed current-time course. An amperometric-ow detector records the resulting mirror symmetrical titration curves, which show two equivalence points. The analyte concentration is calculated from the time difference between the equivalence points. For quantitative conversion of creatinine and quantitative separation of present and released ammonia no calibration is necessary to get accurate results. Both ammonia/ammonium and creatinine were determined in the range between 2mM and 2mM with relative standard deviations between 3.0 and 1.0% (n =5). High recoveries were obtained for the analysis of diluted urine samples for both creatinine and ammonia.
coulometric-ow titration, creatinine iminohydrolase, creatinine, ammonia, calibrationless determinations.,
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