张传溪
病毒分子生物学
个性化签名
- 姓名:张传溪
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
生物化学
- 研究兴趣:病毒分子生物学
张传溪,男,1960年1月出生。1994-1997年在中国科学院上海生物化学研究所从事博士学位论文研究,获浙江农业大学理学博士学位。此后获日本学术振兴会基金资助,在爱媛大学Center of Marine Environmental Studies (CMES)做博士后,从事病毒分子生物学研究。现为浙江大学教授、生物化学与分子生物学专业和农业昆虫与害虫防治专业博士生导师。主要从事昆虫和动物病毒分子生物学与基因工程的研究。研究的目标是阐明昆虫和病毒部分基因的特性和功能及其利用。研究方向包括:杆状病毒和海洋双RNA病毒基因组和基因功能分析、中华蜜蜂蜂毒功能基因组 、昆虫生物反应器(以杆状病毒为载体,以昆虫细胞和家蚕为生物反应器,表达生产人与经济动物药用多肽和科学研究用蛋白)等。先后发表论文100余篇,其中SCI 11篇,个人专著2本。博士学位论文“昆虫杆状病毒ph、pk基因分析和人EPO基因在杆状病毒-昆虫系统中的表达”(高等教育出版社,2001,287页)于2000年被评为全国优秀博士学位论文。入选浙江省“151人才工程”。曾被授予浙江省优秀教师称号。
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张传溪, JIAN-GUO LIN, CHUAN-XI ZHANG, * & SATORU SUZUKI
Virus Genes 31:2, 185-193, 2005,-0001,():
-1年11月30日
VP5 is a 15-kDa nonstructural protein encoded by a small open reading frame in 5
apoptosis,, baculovirus,, MABV,, vp5
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张传溪, D.Wang, , S.-H. An, Z.-J. Guo, H.-J. Xu, and C.-X. Zhang
Arch Virol (2005) 150: 1505-1515,-0001,():
-1年11月30日
Homologues of Helicoverpa armigera nucleopolyhedrovirus (HearSNPV) orf33 are found in all 22 completely sequenced members of the lepidopteran nucleopolyhedroviruses and granuloviruses, but so far their functions are unknown. In this report, we describe the characterization of HearSNPV orf33 (ha33). Northern blot analysis showed a single transcript of ha33 of approximately 0.7 kb was transcribed beginning at 3 h post-infection in infected Helicoverpa zea cells (HzAM1) and the gene product could be detected as early as 6 h post-infection by western blot analysis using a rabbit derived polyclonal antibody, suggesting it was an early gene. Western blot analysis also demonstrated the ha33 protein in infected cellswas a 31 kDa protein, larger than the theoretical size of 28.4 kDa, and located in the envelope fraction of budded virions (BVs). The results suggested that HearSNPV ha33 gene is a functional gene that encodes a novel structural protein of baculovirus BVs, BV-e31.
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【期刊论文】Expression of melittin gene in the venom gland of the Chinese honeybee, Apis cerana cerana1
张传溪, Jiang-Hong LIa, b, Chuan-Xi ZHANGa*, Zhen-hua TANGa, c
Apidologie 36(2005)533-541,-0001,():
-1年11月30日
Melittin is the principal component of bee venom. Melittin in Apis cerana (Ac-melt) is a single copy gene. A full length Ac-melt cDNA is 389 bp, with a single 191 bp intron in the genome. Its mRNA level was high during the first week of adult life and low during the rest of adult life. Melittin or its precursor could not be detected in the pupal stage. Melittin level increased rapidly to its maximum (about 95μg per worker bee) during the first 8-10 days of adult age, and remained constant for about 20days before starting to decline slowly. The expression of melittin is regulated at the transcriptional level.
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张传溪, Jiang-Hong Li, Chuan-Xi Zhang, * Li-rong Shen, Zhen-Hua Tang, and Jia-An Cheng
Archives of Insect Biochemistry and Physiology 60:1-12(2005),-0001,():
-1年11月30日
Phospholipase A2 (PLA2) is one of the components of bee venom with a wide range of pharmacological functions. It operates as a major allergen working with other venom components to defend the colony from intruder. In the present study, the cDNA sequence of the Ac-pla2 gene from cDNA library of the venom gland of Apis cerana was compared with the amplified corresponding region of genomic DNA. The result showed that the Ac-pla2 gene consisted of four exons and three introns. Southern blot showed that the Ac-pla2 gene was a single copy per haploid genome. The most active transcription period was during the first 8 days of adults, which correspondingly was the period of sharp increase of PLA2 protein. ELISA analysis revealed that the PLA2 was undetectable in pupal stage and the newly eclosed adult, but increased sharply to a maximum of 10-12μg per honeybee by 8-10days of adult life, followed by a gradual decrease to 8μg for the rest of adult life. Transcriptional or post transcriptional regulation is the key step for Ac-pla2 expression. The early secreted Ac-PLA2 showed a low degree of posttranslational modification; with increasing age, glycosylation was detected by Western blot and glycoprotein staining analysis. Different post-translational modifications were found among different individuals in A. cerana when compared to A. mellifera. Arch. Insect Biochem. Physiol. 60: 1-12, 2005.
phospholipase A2, expression profile, venom gland, Apis cerana
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张传溪, Zhong-Jian Guo, Shi-Heng An, Dun Wang, Yan-He Liu, V. Shyam Kumar and Chuan-Xi Zhang*
Journal of Biochemistry and Molecular Biology, Vol. 38, No. 3, May 2005, pp. 354-359,-0001,():
-1年11月30日
Open reading frame 29 (ha29) is a gene specific for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearSNPV). Sequence analyses showed that the transcription factor Tfb2 motif, bromodomain and Half-A-TPR (HAT) repeat were present at aa 66-82, 4-76, 55-90 of the Ha29 protein respectively. The product of Ha29 was detected in HearSNPV-infected HzAM1 cells at 3 h post-infection. Western blot analysis using a polyclonal antibody produced by immunizing a rabbit with purified GST-Ha29 fusion protein indicates that Ha29 is an early gene. The size of Ha29 product in infected HzAM1 cells was about 25 kDa, which was larger than the presumed size of 20.4 kDa. Tunicamycin treatment of HearSNPV-infected HzAM1 cells suggested that the Ha29 protein is N-glycosylated. Fluorescent confocal laser scanning microscope examination, and Western blot analysis of purified budded virus (BVs), occlusion-derived virus (ODVs), cell nuclear and cytoplasmic fraction, showed that the Ha29 protein was localized in the nucleus. Our results suggested that ha29 of HearSNPV encodes a non-structurally functional protein that may be associated with virus gene transcription in Helicoverpa hosts.
Expression,, Ha29,, Helicoverpa armigera, Localization,, Single-nucleocapsid nucleopolyhedrovirus
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【期刊论文】HearSNPV orf83 encodes a late, nonstructural protein with an active chitin-binding domain
张传溪, Dun Wang a, b, Chuan-Xi Zhang a, ∗
Virus Research 117(2006)237-243,-0001,():
-1年11月30日
The ORF83 (ha83) of Helicoverpa armigera nucleopolyhedrovirus (HearSNPV) was characterized during the present study. Sequence analysis and chitin-binding assay revealed that Ha83 contained an active chitin-binding domain. Northern blot and Western blot analyses demonstrated that ha83 was expressed as a late gene and encoded a nonstructural protein of HearSNPV. Ha83 gene was transcribed beginning at 12h post-infection in infected Helicoverpa zea cells (HzAM1).Western blot analysis using a rabbit derived polyclonal antibody showed the product of ha83 in infected cells was a 20 kDa protein, in tune with the theoretical size of 18.8 kDa. The protein was first detected in the cytoplasm of infected HzAM1 cells at 12 h p.i., and was transported later into the nucleus during infection.
Nucleopolyhedrovirus, Helicoverpa armigera, HearSNPV orf83, Trans, c, r, i, p, t, ion, Expression, Chitin binding, Cellular localization
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【期刊论文】Bombyx mori nucleopolyhedrovirus ORF79 encodes a 28-kDa structural protein of the ODV envelope
张传溪, H.-J. Xu, Z.-N. Yang, F.Wang, and C.-X. Zhang
Arch Virol (2006) 151:681-695,-0001,():
-1年11月30日
Summary. Open reading frame 79 of Bombyx mori nucleopolyhedrovirus (Bm79) is a conserved gene whose homologues have been identified in all 26 of the completely sequenced baculovirus genomes, including lepidopteran NPVs and GVs, hymenopteran NPVs, and a dipteran baculovirus. Northern blot analysis showed that the Bm79 transcript was about 850 nucleotides long and was initiated 12 h p.i. Temporal expression analysis revealed a 28-kDa protein, which was detected beginning 24 h p.i. using a polyclonal antibody against GST-Bm79 fusion protein. The 28-kDa protein was detected in the occlusion-derived virus envelope (ODV-E), but not in budded viruses. This observation was confirmed by observing ultrathin sections of polyhedra using immunoelectron microscopy. This demonstrated that the protein was present within the nuclei of cells. These results suggest that Bm79 is a functional gene that encodes a structural protein associated with the envelope of occlusion-derived virus (ODV)
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张传溪, , 姜育蕾, 胡萃, 吴祥甫
生物工程学报,2000,(1):46~50,-0001,():
-1年11月30日
人促红细胞生成素(EPO)是一种调控红系干细胞增殖、分化和成熟的糖蛋白激素。将合成的EPO cDNA插入杆状病毒转移载体pBlueBac Ⅲ,使其置于Ph 基因强启动子控制之下,获得了转移载体pBlueBacEPO。将pBlueBacEPO DNA 与野生型BmNPV DNA 共转染BmN 细胞,经空斑纯化,获插入EPO cDNA 的重组病毒rBmN-PVEPO。经Sonthern 杂交和PCR 扩增鉴定证明人EPO 基因已正确组建于BmNPV 的预定位置。将重组病毒rBmNPVEPO 穿刺接种5 龄幼虫和蛹,收集感染第3~5d 的幼虫血淋巴和3~615d 蛹血淋巴。用EL ISA 检测幼虫血淋巴中EPO 表达量高达62800u/mL,蛹血淋巴中表达量达74000u/mL。Western blot 结果显示幼虫血淋巴和蛹血淋巴均有一条明显的免疫杂交带,分子量均约为26kD。用TF21 细胞对幼虫表达产物进行了生物活性测定,每毫升血淋巴中EPO 活性约为63000u。
人促红细胞生长素基因,, 家蚕核型多角体病毒,, 昆虫细胞,, 蚕体,, 基因表达
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