周伟军
作物栽培生理生态、植物生育与化学调控、油菜遗传育种及分子生物学
个性化签名
- 姓名:周伟军
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
农艺学
- 研究兴趣:作物栽培生理生态、植物生育与化学调控、油菜遗传育种及分子生物学
1983年本科、1988年硕士毕业于浙江农业大学、英国伯明翰大学,1998年(在职)获浙江农业大学博士学位,日本宇都宫大学应用生物学博士后毕业。长期在浙江(农业)大学农学系从事作物栽培生理生态、植物生育与化学调控、油菜遗传育种及分子生物学研究工作。1991年任讲师,1994、1998年分别破格晋升为副教授、教授,2000年任博士生导师、浙江大学作物科学研究所副所长。已发表学术论文110余篇(其中SCI论文30篇),主编《作物栽培学》、《跨世纪农业发展研究》等著作。现指导2名硕士生、6名博士生和1名外国博士后,已毕业8名硕士和6名博士,毕业的博士生在国际刊物平均发表SCI论文2.3篇。主持国家自然科学基金、中-英与中-瑞国际合作项目、浙江省科技厅重点项目、教育部博士点基金、浙江省自然科学基金等研究课题。主持育成了油菜高油分新品种"高油605"、油菜早熟新品种"601"并大面积推广,近3年"高油605"省内外收获面积已达413.87万亩, 总增产值高达1.1275亿元,并获2000年浙江省科技进步二等奖和浙江省农业科技进步一等奖,油菜新品种"601"获浙江省科技进步三等奖;油菜生育、化学调控及其应用研究获浙江省教委科技进步三等奖,经济、社会效益也十分显著。还开展了硫代葡萄糖甙等植物次生代谢物、植物间的互作与调控等研究,在国际上首次从寄生植物列当的寄主油菜及模式植物拟南芥根系分泌物中提取、鉴定了根寄生植物的萌发刺激物,并明确寄主的应答反应与分子传递等发育和调控机制,研究结果已发表在2004年4月J. Exp. Bot.(影响因子3.18)等国际刊物。先后在英国伯明翰大学、以色列希伯莱大学、瑞典Svalof Weibull AB、德国基尔大学、英国John Innes Centre、日本宇都宫大学研修及工作,与国外同行建立了很好合作关系。现为J. Integ. Plant Biol., Can. J. Bot., Aus. J. Exp. Agric.等SCI学术刊物审稿人,国际寄生植物研讨会科学委员会委员,中国作物学会油料作物专业委员会理事,浙江省农作物品审委油菜专业组组长,浙江省“新世纪151人才工程”第一层次培养人员等。
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周伟军, W.J. Zhou, *, G.X. Tang and P. Hagberg
Plant Growth Regulation 37: 185-192, 2002,-0001,():
-1年11月30日
The effect of colchicine on embryogenesis induction and chromosome doubling during microspore culture was evaluated in two F1 hybrids of winter oilseed rape (Brassica napus L.). Colchicine treatment (50 and 500 mg/L) of isolated microspores during the firt 15 h in culture stimulated embryogenesis and produced large amounts of healthy-looking embryos. These normal embryos germinated well at 24℃ after being transferred to solid regeneration medium and an initial period of low temperature (2℃) for 10 days, and could directly and rapidly regenerate vigorous plants. A high doubling efficiency of 84-88% was obtained fronl 500 mg/L colchicine treatment for 15h with low frequency of polyploid and chimeric plants. A colchicine treatment duration of 6h was less effective on embryogenesis and doubling efficiency. The present experiment also showed that changing of induction medium 15h after microspore isolation produced higher spontaneous doubling efficiency, as compared with medium change 6h after isolation.
Brassica napus L., ,, Chromosome doubling,, Colchicine,, Doubled haploid,, Embryogenesis,, Microspore culture
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周伟军, W.J. Zhou*, P. Hagberg &G.X. Tang
Euphytica 128: 27-34, 2002,-0001,():
-1年11月30日
The effect of colchicine on induction of embryogenesis and chromosome doubling during microspore culture was evaluated in two F1 hybrids of spring oilseed rape (Brassica napus L.). Immediate colchicine treatment of isolated microspores with the concentrations 50 and 500 mg/L for 15 h stimulated embryogenesis and produced large amounts of healthy-looking embryos. These normal embryos germinated well at 24 ◦C after being transferred to solid regeneration medium and an initial period of low temperature (2 ◦C) for 10 days, and could directly and rapidly regenerate vigorous plants. A high doubling efficiency of 83-91% was obtained from 500 mg/L colchicines treatment for 15 h with low frequency of polyploid and chimeric plants. The present experiment showed that a treatment duration of 30 h revealed less positive effects on embryogenesis and doubling efficiency, especially at higher colchicine concentration (1000 mg/L). Poor embryogenesis and embryo germination were observed from ordinary microspore culture without change of induction medium and colchicine treatment, and several subcultures were required for induction of secondary embryogenesis and plant regeneration.
Brassica napus L., ,, colchicine treatment,, doubled haploid,, embryogenesis,, microspore culture
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周伟军, G.Q. Zhang, G.X. Tang, W.J. Song & W.. J. Zhou*
,-0001,():
-1年11月30日
Using three varieties of Brassica rapa, cv. Hauarad (accession 708), cv. Maoshan-3 (714) and cv. Youbai (715), as the maternal plants and one variety of B. oleracea cv. Jingfeng-1 (6012) as the paternal plant, crosses were made to produce interspecific hybrids through ovary culture techniques. A better response of seed formation was observed when ovaries were cultured in vitro at 9-12 days after pollination on the basal MS and B5 media supplemented with 6-benzylaminopurine (BA) and naphthylacetic acid (NAA). The best response was observed for cross 714×6012 with the rate of seeds per ovary reaching 43.0%. Seeds for cross 715×6012 showed the best germination response (66.7%) on the regeneration medium (MS + 1.0 mg l−1 BA + 0.05 mg l−1 NAA). In all three cross combinations, good response in terms of root number and length of plants was observed on the root induction medium (MS+1.0 mg l−1 BA + 0.1 mg l−1 NAA). A better response was observed for the regenerated plants cultured for 14 days than for 7 days. The ovary-derived plants with well-developed root system were hardened for 8 days and their survival rate reached over 80%. Cytological studies showed that the chromosome number of all plants tested was 19 (the sum of both parents), indicating that these regenerated plants were all true hybrids of B. rapa (n=10)×B. oleracea (n=9). The regenerated plants were doubled with colchicine treatment, and the best response in the crosses 708×6012, 714×6012 and 715×6012 was observed when treated with 170 mg l−1 colchicine for up to 30 h and their doubling frequency reached 52, 56 and 62%, respectively.
Brassica rapa,, B., oleracea,, interspecific hybridization,, ovary culture,, plant regeneration,, synthetic B., napus
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周伟军, E J J MOMOH*, W J ZHOU* & B KRISTIANSSON
,-0001,():
-1年11月30日
A substantial amount of seed is left in the fields before and during harvest of oilseed rape. Although this crop exhibitslittle or no primary dormancy, the absence of certain environmental cuesthat promote germination of imbibed seeds induces secondary dormancy. The work reported investigated the extent to which environmental stress conditions, including osmotic stress, low oxygen stress and anaerobiosis, induce secondary dormancy in oilseed rape, and examined the ariation in development of secondary dormancy between and within genotypes. Osmotic stress was most effective in inducing dormancy. Anaerobic treatment produced very few dormant seeds, asdid an atmosphere low in oxygen and high in nitrogen. The development of secondary dormancy under osmotic stress varied considerably between and within genotypes. Dormancy ranged from almost zero to about 60% for winter genotypesand about 85% for spring types. Within genotypes, variations occurred between seed lots and years of harvest. Temperature variations affected the percentage of dormant seeds. More dormant seeds were likely to be produced with incubation under water stress at 20℃ than at 12℃. In winter genotypes, fewer dormant seeds were produced when incubation temperature and germination test temperaturesdiffered. Thus, incubating at 20℃ and 12℃, followed by germination tests at 20℃and 12℃, respectively, produced most dormant seeds. Also, in the winter genotypes, the potential development of secondary dormancy waspos itively correlated with the pattern and speed of germination of untreated seeds.
water stress,, volunteer rape,, Brassica napus,, seed germination.,
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【期刊论文】In vitro infection of host roots by differentiated calli of the parasitic plant Orobanche
周伟军, W. J. Zhou, , K. Yoneyama , Y. Takeuchi, S. Iso, S. Rungmekarat, S. H. Chae, D. Sato and D. M. Joel, *
Journal of Experimental Botany, Vol. 55, No.398, pp. 899-907, April 2004,-0001,():
-1年11月30日
Root parasites of the genus Orobanche are serious weeds in agriculture. An aseptic infection system of host roots using calli of three Orobanche species was developed for the study of host
Aseptic infection,, Brassica,, callus induction,, germination,, Orobanche,, red clover,, tomato.,
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【期刊论文】Medium, Explant and Genotype Factors Influencing Shoot Regeneration in Oilseed Brassica spp.
周伟军, G. X. Tang, W. J. Zhou, H. Z. Li, B. Z. Mao, Z. H. He and K. Yoneyama
J. Agronomy & Crop Science 189, 1-8 (2003),-0001,():
-1年11月30日
The effects of culture media, explants and genotypes on shoot regeneration in oilseed Brassica species were examined in this study. The maximum shoot regeneration 3frequency was obtained in Murashige and Skoog medium supplemented with 3 mg l)1 6-benzylaminopurine and 0.15 mg l)1 1-naphthaleneacetic acid. The addition of 2.5mg l)1 AgNO3 was very beneficial to shoot regeneration in B. napus and Ag2S2O3 (10mg l)1) was even superior to AgNO3 (2.5 mg l)1). Explant age, explant type and carbon source also significantly affected shoot regeneration. Four-day-old seedlings of cotyledonary explants showed the maximum shoot regeneration frequency and number of shoots per explant. Of the four explants-peduncles, hypocotyls, cotyledons and leaf petioles-cotyledons produced the highest shoot regeneration frequency (56.67%). Four carbon sources-glucose, maltose, starch and sucrose-were compared for their respective effects on shoot regeneration from cotyledonary explants. Sucrose appeared to be the best carbon source for shoot regeneration with the highest shoot regeneration frequency (76.00%). Considerable variation in shoot regeneration from cotyledonary explants was observed both between and within Brassica species. The shoot regeneration frequency ranged from 10.00% for cv. R5 (B. rapa) to 83.61% for cv. N1 (B. napus). Two B. napus, one B. carinata and one B. juncea cultivars exhibited shoot regeneration frequency higher than 70 %. In terms of the number of shoots produced per explant, B. rapa showed the highest variation, ranging from 5.64 for cv. R3 to 1.33 for cv. R5. Normal plantlets were regenerated from all induced shoots and developed normally. The 4regenerated plants were fertile and identical with the source plants.
Ag+, , Brassica species, carbon sources, explants, genotypes, shoot regeneration
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周伟军, G. Q. Zhang, W. J. Zhou, H. H. Gu, W. J. Song, and E. J. J. Momoh
J. Agronomy & Crop Science 189, 1-4 (2003),-0001,():
-1年11月30日
Crosses were made to produce interspecific hybrids between Brassica napus • B. juncea and their reciprocals with the aid of embryo culture techniques. A better response of hybrid embryo culture was obtained from 4two cross combinations of B. juncea • B. napus (Ames 24521 • Huyou 15 and Vittasso • Zheshuang 72) than from their reciprocals. Embryo culture was more effective in terms of plant regeneration when embryos were cultured in vitro at 15 days after pollination (DAP), while more calli were initiated when embryos were excised and cultured at 10 DAP. A better response was observed on the MS medium with 0.3 mg l)1 naphthylacetic acid (NAA) + 1.5 mg l)1 6-benzylaminopurine (BAP) and with 0.3 mg l)1 NAA + 2.0 mg l)1 BAP. Callus formation and plant regeneration on these two media reached 55.43 and26.65 %, and 66.98 and 24.61 %, respectively.
Brassica juncea, B., napus, embryo Culture, interspecific hybridization
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【期刊论文】Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.)
周伟军, H.H. Gu, , P. Hagberg and W.J. Zhou, *
Plant Growth Regulation 42: 137-143, 2004,-0001,():
-1年11月30日
Stress is an essential component during embryogenesis induction in microspore culture. Cold pretreatment has been used in cereal microspore culture but very seldom attempted in Brassica microspore culture. The effect of cold pretreatment of flower buds subjected to a liquid medium on microspore embryogenesis was investigated in spring and winter Brassica napus, as well as in B. rapa and B. oleracea. Cold pretreatment significantly enhanced microspore embryogenesis (by 1-7 fold) compared to commonly used microspore culture protocol in B. napus, while it was less effective in B. rapa or even negative in B. oleracea. The appropriate duration of cold pretreatment was found to be 2-4 days, which stimulated the best microspore embryogenesis. Cold pretreatment was also able to promote embryo development including the improvement of embryo quality and acceleration of embryogenesis. When incorporating with medium refreshing, coldpretreatment could initiate the most microspore embryogenesis than any other treatment used. With further improvement cold pretreatment method may have a positive potential in Brassica breeding programmes.
Brassica napus,, B., oleracea,, B., rapa,, Cold pretreatment,, Embryogenesis,, Microspore culture
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周伟军, H.H. Gu, , W.J. Zhou, * & P. Hagberg
Euphytica 134: 239-245, 2003,-0001,():
-1年11月30日
Brassica rapa ssp., chinensis,, doubled haploid,, embryogenesis,, microspore culture
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周伟军, Melvin Sidikie George, Guoquan Lu, Weijun Zhou
Field Crops Research 77(2002)7-15,-0001,():
-1年11月30日
Field experiments were conducted to study genotypic variation for potassium uptake and utilization efficiency in sweet potato for 2 years. In 1997, a field experiment was conducted using 84 genotypes under no potassium fertilization. In 1998, two field experiments were carried out. In experiment I, eight good genotypes evaluated from the previous experiment were tested at two K application levels: 0 and 270 kg ha-1 of K2SO4. In experiment II, three special varieties with respect to pigment content were tested at five K levels from 0 to 600 kg ha-1. A split-plot design with three replications was used. Variation existed among genotypes in K concentration, accumulation and potassium efficiency ratio (KER) in the field. Among various plant parts, petiole contained the highest K concentration and storage roots had the highest K accumulation at maturity. These changed considerably with genotype and K level. KER had significant positive correlation with root weight per plant, root:top ratio and harvest index (HI); and significantly negative correlation with K concentration and accumulation in the roots or whole plant at maturity. Based on KER, genotypes were identified as efficient and inefficient in potassium utilization. Yield increased generally in all genotypes with K application. The increase, however, varied among different genotypes. It was also found from this study that the fertilizer level that gave the highest yield was K2 which is 300 kg ha-1 of KzSO4. The yield increase as a result of K application was mainly due to the increase in root:top ratio which led to greater amount of photosynthate translocation to the storage roots causing their increase in size. Most of the quality parameters tested increased with increasing levels of K nutrition. Root dry matter (%), Brix (%), carotene content, anthocyanin content increased with K application. The extent of increase, however, differed with genotypes. Protein content generally tended to reduce with increase in K level. The degree of reduction also varied with genotypes.
Sweet potato (, Ipomoea batatas L., ), , Potassium nutrition, Genotypic difference, Fertilizer efficiency, Fertilizer utilization efficiency
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