张承才
蓝细菌的“与细胞间相互作用有关的信号转导机制”和“细菌分化和细菌分裂的关系”等方面
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- 姓名:张承才
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博士生导师
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学科领域:
细胞生物学
- 研究兴趣:蓝细菌的“与细胞间相互作用有关的信号转导机制”和“细菌分化和细菌分裂的关系”等方面
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【期刊论文】Obligate phototrophy in cyanobacteria: more than a lack of sugar transport
张承才, Cheng-Cai Zhang a, Robert Jeanjean b, Fran
FEMS Microbiology Letters 161(1998)285-292,-0001,():
-1年11月30日
DNA hybridization using the Synechocystis PCC6803 glucose transporter gene, glcP, revealed a single homologous region in two facultative photoautotrophic strains out of three tested, and none in three obligate autotrophs. In one of the latter, Synechococcus PCC7942, integration of glcP into the chromosome resulted in glucose sensitivity. A subclone isolated as glucose-tolerant had lost glcP. Integration in a replicative vector allowed glucose transport and photoheterotrophic growth, but could not be maintained. Thus lack of sugar transport could explain cyanobacterial obligate autotrophy. However, at least in Synechococcus PCC7942, acquisition of such a transport capacity created a metabolic disequilibrium barely compatible with survival.
Cyanobacteria, Glucose transport, Heterotrophy
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张承才, CHENG-CAI ZHANG, * ALINE FRIRY, † AND LING PENG
JOURNAL OF BACTERIOLOGY, May 1998, p. 2616-2622,-0001,():
-1年11月30日
Reversible protein phosphorylation plays important roles in signal transduction. One gene, prpA, encoding a protein similar to eukaryotic types of phosphoprotein phosphatases PP1, PP2A, and PP2B, was cloned from the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. Interestingly, a eukaryotic-type protein kinase gene, pknE, was found 301 bp downstream of prpA. This unusual genetic arrangement provides the opportunity for study about how the balance between protein phosphorylation and dephosphorylation can regulate cellular activities. Both proteins were overproduced in Escherichia coli and used to raise polyclonal antibodies. Immunodetection and RNA/DNA hybridization experiments suggest that these two genes are unlikely to be coexpressed, despite their close genetic linkage. PrpA is expressed constitutively under different nitrogen conditions, while PknE expression varies according to the nature of the nitrogen source. Inactivation analysis in vivo suggests that PrpA and PknE function to ensure a correct level of phosphorylation of the targets in order to regulate similar biological processes such as heterocyst structure formation and nitrogen fixation.
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张承才, Li Wang a, Yi-Ping Sun a, Wen-Li Chen a, Jian-Hong Li b, Cheng-Cai Zhang a, b, *
FEMS Microbiology Letters 217(2002)155-165,-0001,():
-1年11月30日
Anabaena sp. PCC 7120 is a cyanobacterium capable of performing several important biological functions: photosynthesis, nitrogen fixation, cell differentiation, cell-cell communication, etc. These activities require an extensive signaling capability in order to respond to the changing environment. Based on the genomic data, we have retrieved several gene families encoding signaling components. It is estimated that 211 genes encode two-component signaling elements, and 66 genes encode Ser/Thr kinases and phosphatases. These genes together represent 4.2% of the coding capacity of the whole genome, making Anabaena PCC 7120 a leading member among prokaryotes in terms of its signaling potential. It is known that two-component systems are composed of a few basic modules that can arrange into different structures best adapted for each signaling system. Many proteins in Anabaena PCC 7120 have incorporated both modules of two-component systems and catalytic domains of either Ser/Thr kinases or phosphatases. A family of 13 genes encode proteins with both a Ser/Thr kinase domain and a His kinase domain, and another four genes were also found whose products have both a response regulator domain and a Ser/Thr phosphatase domain. Of all the signaling proteins in Anabaena PCC 7120, about one third (35%) are conserved in the genome of the unicellular cyanobacterium strain Synechocystis sp. PCC 6803. Interestingly, one subfamily of His kinases and two subfamilies of response regulators are found in Anabaena PCC 7120 but are absent in Synechocystis PCC 6803. This study constitutes a basis for analyses of signal transduction in Anabaena PCC 7120 using functional genomic approaches.
Protein phosphorylation, Signal transduction, Evolution, Genome, Two-component system
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张承才, Wen-Liang Xu a, Robert Jeanjean b, Yong-Ding Liu a, Cheng-Cai Zhang b, c, *
FEBS Letters 553(2003)179-182,-0001,():
-1年11月30日
In cyanobacteria, the isiA gene is required for cell adaptation to oxidative damage caused by the absence of iron. We show here that a putative Ser/Thr kinase gene, pkn22 (alr2052), is activated by iron deficiency and oxidative damage in Anabaena sp. PCC 7120. A pkn22 insertion mutant is unable to grow when iron is limiting. pkn22 regulates the expression of isiA (encoding CP43P), but not of isiB (encoding flavodoxin) and psbC (CP43). Fluorescence measurement at 77 K reveals the absence of the typical signature of CP43P associated with photosystem I in the mutant under iron-limiting conditions. We propose that Pkn22 is required for the function of isiA/CP43P and constitutes a regulatory element necessary for stress response.
Signal transduction, Iron, Photosynthesis, Protein phosphorylation
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张承才, ISABELLE KUHN, LING PENG, SYLVIE BEDU, AND CHENG-CAI ZHANG, *
JOURNAL OF BACTERIOLOGY, Aug. 2000, p. 4640-4643,-0001,():
-1年11月30日
Heterocysts are terminally differentiated cells devoted to nitrogen fixation in the filamentous cyanobacterium Anabaena sp. strain PCC 7120. We show here that the cell division protein FtsZ is present in vegetative cells but undetectable in heterocysts. These results provide a first rational explanation for the inability of mature heterocysts to undergo cell division.
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张承才, C.-C. Zhang
Mol Gen Genet (1998) 258: 26-33,-0001,():
-1年11月30日
Protein phosphorylation catalysed by protein kinases is an important mechanism for signal transduction in both prokaryotes and eukaryotes. A novel gene, pknD, encoding a protein similar to eukaryotic-type protein kinases, was cloned from Anabaena sp. PCC7120. The N-terminal region of PknD is 60% identical to that of PknA, another putative Ser/Thr kinase from the same strain. Both PknA and PknD have C-terminal regions that are rich in Pro and Thr residues. Expression of pknD was undetectable by RNA/DNA hybridisation and was thus examined by RT-PCR. The pknD transcript was detected in filaments cultured in the presence of either nitrate or ammonium as a source of combined nitrogen, and also in filaments transferred from nitrate-su
Protein kinase
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张承才, Leticia Gonzalez, Vincent Phalip and Cheng-Cai Zhang
Eur. J. Biochem. 268, 1869-1875 (2001),-0001,():
-1年11月30日
Eukaryotic-like protein Ser/Thr and Tyr kinases have only recently been discovered in prokaryotes. In most cases, their biochemical properties have been poorly characterized. The nitrogen-fixing and heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 houses a family of eukaryotic-like Ser/ Thr kinases. Some of these enzymes are required for cell growth or development under certain conditions. None of them, however, has been shown experimentally to possess Ser/Thr kinase activity. A gene, pknC, encoding a novel putative Ser/Thr kinase was isolated from Anabaena sp. PCC 7120. The recombinant PknC was shown to be phosphorylated on a Thr residue. This phosphorylation was probably due to the autophosphorylation activity of PknC itself because mutation of two amino acid residues within the subdomain II of its catalytic domain eliminated the phosphorylation of PknC. PknC displayed also a Ser kinase activity towards several nonspecific substrates, and the two residues needed for PknC autophosphorylation was equally required for the phosphorylation of other substrates. PknC is thus a Ser/Thr kinase with broad substrate specificity. The activity of PknC is likely to be regulated in vivo in order to limit the spectrum of its substrate specificity.
autophosphorylation, heterocyst, His Tag, protein phosphorylation, signal transduction.,
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张承才, Vincent PHALIP*, Jian-Hong LI†‡ and Cheng-Cai ZHANG†‡
Biochem. J.(2001) 360, 639-644 (Printed in Great Britain),-0001,():
-1年11月30日
Two distinct families of protein kinases are involved in signal transduction: Ser, Thr and Tyr kinases, which are predominantly found among eukaryotes, and His kinases, as part of bacterial two-component signalling systems. Genetic studies in Arabidopsis and Saccharomyces have demonstrated that bacterial-type twocomponent systems may act upstream of Ser/Thr kinases in the same signalling pathway, but how this coupling is accomplished remains unclear. In the present study, we report the characterization of a protein kinase, HstK, from the N2-fixing cyanobacterium Anabaena sp. PCC 7120, that possesses both a Ser/Thr kinase domain and a His kinase domain. Proteins with a structural architecture similar to that of HstK can be found in the eukaryote, Schizosaccharomyces pombe, and the bacterium, Rhodococcus sp. M5. HstK was present in cells grown with NH4+ or N2 as the nitrogen source, but was absent in cells grown with NO-3. The hstK gene was inactivated and the mutant phenotype was characterized. The catalytic domain of the Ser/Thr kinase of HstK functionally replaced that of Hog1p, a well-characterized protein kinase required for the response to high osmolarity in the S. cerevisiae heterologous system. The unusual multidomain structure of HstK suggests that a two-component system could be directly coupled to Ser/Thr kinases in the same signal transduction pathway.
Anabaena,, mitogen-activated protein kinase,, protein kinase,, signal,, ransduction,, two-component system.,
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张承才, C.-C. Zhang (*), S. Huguenin and A. Friry
Res. Microbiol. 1995, 146: 445-455,-0001,():
-1年11月30日
Heterocysts, cells specialized in nitrogen fization in anabaena sp. PCC7120, lose the potential for cell division once fully differentiated. This suggests that cell divisoion activity is differentlally regulated in heterocysts and vegetative cells. FtsZ has been shown to play a crucial role in bacterial cell division. Two degenerate oligonucieotide primers were designed to detect, by polymerase chain reaction (PCR). an ftsZ homologue from the heterocystous syanobacterium Anabaena sp. PCC 7120. A PCR-amplified DNA fragment was cloned and used as a probe to isolate the entire ftsZ gene of Anabaena sp. PCC 7120. The deduced amino acid sequence shares strong similarities with other FtsZ protains, suggesting remarkable conservation of the FtsZ protein during evolution. An ORF dcwnstream of ftsZ. wjocj would be transcribed in the opposite direction com pared to ftsZ, could encode a polypeptide with significant sequence similarity to the glutathione synthetase from Escherichia coli. Inactivation experiments in vivo for both ftsZ and the glutathione synthetase gene did not yield any double recombinants either in the presence or in the absence of combined nitrogen, suggesting that both genes are essential for cell growth under these conditions.
Heterocyst,, Anabaena,, FtsZ,, Nitrogen fization, Cell division,, ftsZ,, Glutathione synthetase,, Inactivation.,
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张承才, Jian-Hong Li, Sophie Laurent, Viren Konde, Sylvie B
Microbiology (2003), 149, 3257-3263,-0001,():
-1年11月30日
In the filamentous cyanobacterium Anabaena sp. strain PCC 7120, a starvation of combined nitrogen induces differentiation of heterocysts, cells specialized in nitrogen fixation. How do filaments perceive the limitation of the source of combined nitrogen, and what determines the proportion of heterocysts? In cyanobacteria, 2-oxoglutarate provides a carbon skeleton for the incorporation of inorganic nitrogen. Recently, it has been proposed that the concentration of 2-oxoglutarate reflects the nitrogen status in cyanobacteria. To investigate the effect of 2-oxoglutarate on heterocyst development, a heterologous gene encoding a 2-oxoglutarate permease under the control of a regulated promoter was expressed in Anabaena sp. PCC 7120. The increase of 2-oxoglutarate within cells can trigger heterocyst differentiation in a subpopulation of filaments even in the presence of nitrate. In the absence of a source of combined nitrogen, it can increase heterocyst frequency, advance the timing of commitment to heterocyst development and further increase the proportion of heterocysts in a patS mutant. Here, it is proposed that the intracellular concentration of 2-oxoglutarate is involved in the determination of the proportion of the two cell types according to the carbon/nitrogen status of the filament.
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