张志琪
长期从事分析化学研究工作,研究领域涉及分离科学、流动注射分析等
个性化签名
- 姓名:张志琪
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
分析化学
- 研究兴趣:长期从事分析化学研究工作,研究领域涉及分离科学、流动注射分析等
张志琪,男,1958年12月出生,博士,教授,博士生导师。1984年12月毕业于陕西师范大学分析化学专业,获理学硕士学位;1996年6月晋升教授职称;1999年获国务院政府特殊津贴;2004年在职于西北大学获博士学位。2006.09 -2007.08在美国能源部Ames实验室和美国天然产物国家研究中心访学,从事单分子单细胞测定和天然药物筛选研究。
现任陕西师范大学药用植物资源与天然药物化学教育部重点实验室副主任,兼任陕西省化学学会常务理事,陕西省化学学会教育委员会主任,陕西省计量测试学会化学专业委员会副主任。
长期从事分析化学研究工作,研究领域涉及分离科学、流动注射分析等,主持或参加完成的三项科研成果分别获陕西省和教育部科技成果奖。在Anal. Chim. Acta、Anal. Bioanal. Chem.、J. Chromatogr. Sci.、Chromatogr.、Talanta、Mikrochim. Acta、化学学报、科学通报、高等学校化学学报、分析化学、中国中药杂志、中草药、天然产物开发与研究、药物分析杂志等国内外期刊发表学术论文80多篇。
近年来在两项国家自然科学基金项目(“中药川芎及复方细胞膜受体作用活性成分的研究”和“VEC受体药物的色谱筛选模型研究及太白‘七药’活性成分的应用研究”)、一项教育部科技重点项目(以细胞膜受体作靶标筛选中药有效成分)、一项教育部博士点基金项目(用于治疗心血管疾病药物筛选的生物亲和色谱研究)的支持下,主要从事以生物亲和色谱和基因芯片筛选、分离中药活性成分研究;以超临界、微波等现代提取手段结合分离、纯化、制备、表征技术的中药化学成分研究;以及中药质量控制和药物分析的研究工作。此外,还参加一项国家863高新技术项目和一项国家973重大基础研究前期项目。
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主页访问
1597
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成果阅读
241
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成果数
5
张志琪, Zhi-Qi Zhang∗, Guo-Xi Liang
Analytica Chimica Acta 536(2005)145-151,-0001,():
-1年11月30日
The mechanism of binding of carbamazepine (CBZ) with bovine serum albumin (BSA) has been investigated in vitro based on a new flow injection fluorometry coupled to the technique of dialysis sampling. The CBZ and BSA were mixed in different molar ratios in 0.050 mol L-1 phosphate buffer (containing 0.9% NaCl), pH 7.4, and incubated at 37±0.5℃ in a water bath. The dialysis sampler was utilized to sample free CBZ from mixed solution with a relative dialytic efficiency of 7.6%. Then the CBZ in dialysis solution was injected into carrier and on-line oxidized by lead dioxide solid-phase reactor into fluorescent product with a maximum excitation wavelength of 355 nm and a maximum emission wavelength of 478nm. The fluorescence intensity measured was linear proportional with the concentration of free CBZ in mixed solution over the range of 1×10-5 to 2×10-4mol L-1 with the detection limit of 6×10-6 mol L-1. According to the fluorescence measurement results from mixed solution, the association constant (K) estimated for CBZ–BSA binding and the number of the binding site (n) with Scatchard analysis were 1.08×104 L mol-1 and 0.94, respectively. Stern–Volmer plots indicated the presence of dynamic component in the quenching mechanism. The acting force was suggested to be mainly hydrophobic and the distance between the acceptor and donor was 3.12 nm. The estimated binding parameters agreed well with literature values.
Flow injection fluorometry, Dialysis sampling, Carbamazepine, Bovine serum albumin, Binding parameters
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张志琪, Zhi-Qi Zhang, Ying Tang
Anal Bioanal Chem (2005) 381: 932-936,-0001,():
-1年11月30日
A simple, sensitive and specific flow-injection spectrofluorimetric method has been developed for the determination of folic acid in pharmaceuticals. The method is based on use of a lead dioxide solid-phase reactor for on-line oxidation of folic acid into a strongly fluorescent compound with a maximum excitation wavelength of 281nm and an emission wavelength of 450 nm. Under optimum conditions the fluorescence intensity of oxidation product is proportional to the concentration of folic acid over the range 0.008-2.5ug mL-1. The detection limit is 0.0001ug mL-1, the relative standard deviation is 0.85% for 11 replicate determinations of 0.05ug mL-1 folic acid, and the sample throughput is 20h-1. In combination with an on-line filter and dilution, an automated drug-dissolution system was established. The proposed method has been successfully applied to the determination of folic acid in pharmaceutical preparations and dissolution testing.
Folic acid, Flow-injection analysis, Spectrofluorimetry, Dissolution, Solid-phase reactor
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张志琪, Zhi-Qi Zhang, , Hong-Tao Yan, and Xuan-Feng Yue
Microchim. Acta 146, 259-263 (2004),-0001,():
-1年11月30日
A sensitive flow injection method is proposed for the determination of formaldehyde based on its catalytic action upon the redox reaction between crystal violet and potassium bromate in phosphoric acid medium. The reaction is monitored spectrophotometrically by measuring the decrease in absorbance of crystal violet injected at the maximum absorption wavelength of 600nm. A calibration graph from 0.018 to 1.400mgmL 1 is obtained, and the detection limit is 0.007mgmL 1 in a sample volume of 30 mL. Up to 15 samples can be analyzed per hour with a relative standard deviation of 1.2 and 2.3%, respectively, for the determination of 1.0 and 0.1mgmL 1 of formaldehyde. The method is free from most interference. This method has been applied to the determination of formaldehyde in air, decoration boards and paints, and the results compare well with those provided by iodimetry.
Catalytic determination, formaldehyde, spectrophotometry, flow injection analysis
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【期刊论文】Flow injection catalytic spectrophotometric simultaneous determination of nitrite and nitrate
张志琪, Xuan-Feng Yue a, Zhi-Qi Zhang a, b, ∗, Hong-Tao Yan b
Talanta 62(2004)97-101,-0001,():
-1年11月30日
A new flow injection catalytic spectrophotometric method is proposed for the simultaneous determination of nitrite and nitrate based on the catalytic effect of nitrite on the redox reaction between crystal violet and potassium bromate in phosphoric acid medium and nitrate being on-line reduced to nitrite with a cadmium-coated zinc reduction column. The redox reaction is monitored spectrophotometrically by measuring the decrease in the absorbance of crystal violet at the maximum absorption wavelength of 610 nm. A technique of inserting a reduction column into sampling loop is adopted and the flow injection system produces a signal with a shoulder. The height of shoulder in the ascending part of the peak corresponds to the nitrite concentration and the maximum of the peak corresponds to nitrate plus nitrite. The detection limits are 0.3ng ml-1 for nitrite and 1.0 ng ml-1 for the nitrate. Up to 32 samples can be analyzed per hour with a relative standard deviation of less than 2%. The method has been successfully applied for the simultaneous determination of nitrite and nitrate in natural waters.
Catalytic spectrophotometry, Flow injection analysis, Nitrate, Nitrite, Simultaneous determination
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张志琪, Zhi-Qi Zhang, Xiao-Qin Xu
Analytica Chimica Acta 406(2000)303-308,-0001,():
-1年11月30日
A flow-injection catalytic method is developed for the determination of oxalic acid based on its catalytic effect on the redox reaction between Victoria blue B and potassium dichromate in dilute sulfuric acid medium. The reaction is monitored spectrophotometrically by measuring the decrease in the absorbance ofVictoria blue B at the maximum absorptionwavelength of 618 nm. A calibration graph from 1.0 to 80.0 mgml-1 of oxalic acid and a detection limit of 0.8 mgml-1 was obtained. The sampling rate was about 22 samples per hour. The proposed method is simple and inexpensive. The applicability of the method was demonstrated by the determination of oxalic acid in vegetable food, such as spinach and mushroom.
Oxalate, Catalytic determination, Flow-injection analysis, Spectrophotometry, Food analysis
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