高大文
主要研究领域为水污染控制及环境微生物技术,包括污水生物处理理论与新工艺、膜法水处理理论与技术、土壤污染修复理论与技术等
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- 姓名:高大文
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博士生导师, 教育部“新世纪优秀人才支持计划”入选者
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环境工程学
- 研究兴趣:主要研究领域为水污染控制及环境微生物技术,包括污水生物处理理论与新工艺、膜法水处理理论与技术、土壤污染修复理论与技术等
高大文,东北林业大学教授,博士生导师。2003年获哈尔滨工业大学博士学位,所撰写的博士论文获2005年全国百篇优秀博士学位论文。2003年-2005年在清华大学环境科学与工程系从事博士后研究。2005年入选“教育部新世纪优秀人才支持计划”。2006年获黑龙江省第七届青年科技奖。2006年-2007年在美国斯坦福大学土木与环境工程系从事博士后/访问学者研究。现为美国微生物学会会员,国际水协会会员,加拿大国际环境信息科学学会会员,中国土木工程学会会员。主要研究领域为水污染控制及环境微生物技术,包括污水生物处理理论与新工艺、膜法水处理理论与技术、土壤污染修复理论与技术等。主持和参加科研课题20余项,其中主持的项目有国家自然科学基金、教育部新世纪优秀人才支持计划项目、全国优秀博士学位论文专项研究基金、黑龙江省自然科学基金资助项目和中国博士后科学研究基金项目等10余项;参加的项目有国家自然科学基金项目5项和国家科技部863项目2项,另外,作为专家参加中国工程院重大咨询项目2项。在国内外学术期刊上发表论文60余篇,授权国家专利4项,主编和参编教材2部。曾获黑龙江省科学技术二等奖(自然类)1项,黑龙江省高校科技进步二等奖1项。
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【期刊论文】Competition strategies for the incubation of white rot fungi under non-sterile conditions
高大文, Dawen Gao a, b, *, Yonggang Zeng a, Xianghua Wenb, Yi Qian b
Process Biochemistry 43(2008)937-944,-0001,():
-1年11月30日
White rot fungi is capable of oxidizing many persistent organic pollutants including dyes. The application of the fungi has been limited for wastewater treatment under non-sterile conditions due to the contamination by bacteria and other micro-organisms. We developed a treatment approach by using immobilized white rot fungi Phanerochaete chrysosporium to degrade reactive dye K-2BP under nonsterile condition. Four different inert carriers were tested for immobilization of the white rot fungi with orthogonal experiments in comparison with suspension culture. The activity of manganese peroxidase (MnP) was used for the evaluation of oxidization performance in order to understand whether contamination of bacteria and other micro-organisms was suppressed. Under non-sterile conditions, the immobilized fungal cultures successfully restrained the growth of microzymes, coccies, and bacillus but suspension culture was highly contaminated with poor MnP activity. Under non-sterile conditions, higher MnP enzymatic activity (690 U/L vs. 125 U/L), higher decolorization efficiency (93.5% vs. 15%) and shorter reaction period (3 days vs. 6 days) were achieved in immobilized cultures in comparison with suspension culture. With the immobilized fungal cultures, no difference was observed under non-sterile and sterile conditions for the degradation of reactive dye K-2BP.
Phanerochaete chrysosporium, Immobilization, Non-sterile condition, Suspended culture, Reactive dyes, Decolorization, Bacterial contamination
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高大文, GAO DaWen, , WEN XiangHua? & QIAN Yi
GAO DaWen et al. Sci China Ser B-Chem | Oct. 2007 | vol. 50 | no. 5 | 718-724,-0001,():
-1年11月30日
In order to explore ways for the application of white rot fungus in dye effluent treatment under non-sterile conditions, experiment on decolorization of reactive brilliant red was carried out, employing nitrogen-limited and carbon-limited medium with C/N ratio of 56/2.2 and 28/44 (in mmol/L), respectively. The results showed that the decolorization rate reached 92% while culturing white rot fungus with nitrogen-limited medium; however, the decolorization process ended in carbon-limited medium (n(C)/n(N) = 28/44) because of bacterial contamination. In addition, pH rose up to 9.31 after 4 d of decolorization, which was caused by bacterial contamination in the carbon-limited system. Therefore, it is concluded that nitrogen-limited medium can inhibit bacterial growth to some extent while carbon-limited medium is more easily contaminated by bacteria. Nitrogen-limited medium is more suitable in culture of white rot fungus for decolorization of reactive dye. Medium with the ability of inhibiting yeast growth should be developed by adjusting other components of nitrogen-limited medium.
white rot fungus, Phanerochaete chrysosporium, non-sterile, decolorization, dye
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高大文, GAO Da-wen*, WEN Xiang-hua, QIAN Yi
Journal of Environmentul Sciences Vol.18, No.3, pp. 428-432, 2006,-0001,():
-1年11月30日
Almost all the studies both domestic and international using white rot fungus for bye wastewater treatment are performed under sterile conditions. However, it is obviously unpractical that wastewater with dyes is treated under sterile conditions. A feasible study was made for using white rot fungus phanerochaete chrysosporium to degrade reactive brilliant red L-2BP dye under non-sterile conditions. The results showed that there was no decolorizing effect under non-sterile condition if white rot fungus was incubated under non-sterile condition, and the decolorization was always near to 0% during decolorizing test for 3 d; in the meantime, a lot of yeast funguses were found in liquid medium when white rot fumgus was incubated under non-sterile conditions; however, if white rot fungus was incubated under sterile condition firstly, its decolorization was above 90% under non-sterile condition, which was similar to that of sterile condition. So we point out that the treating process for wastewater with dyes should be divided into two stage. The first stage is that white rot fungus should be incubated under stcrile conditions, and the second stage is that reactive brilliant red K-2BP is decolorized under non-sterile conditions. The method not only save that operation cost which decolorizing reactive brilliant red K-2BP under sterile condition, but also provide that feasibility for using white rot fungus to degrade wastewater with dyes udder non-sterile conditions.
White rot fungus, Phanerochaete chrysosporium, reactive brilliant red K-2BP, decoloriation, non-sterile condition
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高大文, GAO Dawen, WEN Xianghua & QIAN Yi
Chinese Science Bulletin 2004 Vol. 49 No.9 981-982,-0001,():
-1年11月30日
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