徐志康
高分子分离膜及相关分离技术的基础研究
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- 姓名:徐志康
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学术头衔:
博士生导师
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学科领域:
高分子化学
- 研究兴趣:高分子分离膜及相关分离技术的基础研究
徐志康,男,1991年5月获浙江大学理学博士学位,1998年晋升为浙江大学高分子系教授,2006年获国家杰出青年基金,现为TOP期刊J. Membr. Sci.编委。1994至1995年在德国柏林工业大学从事访问研究,1999年、2001年、2003年曾分别应邀访问德国柏林工业大学、法国国家科学研究中心欧洲膜研究所、德国埃森大学,进行学术交流和为期3个月的合作研究。主要从事高分子分离膜及相关分离技术的基础研究,研究兴趣涉及:[1] 高分子分离膜的表面与界面构建、[2] 仿生和生物功能分离膜的设计与制备基础、[3] 环保领域中的膜分离技术。参与出版中英文专著5部,作为第一作者或通讯作者发表期刊论文180多篇,其中SCI收录论文110余篇(其中影响因子3.0以上期刊论文40余篇)、EI收录论文90余篇。获教育部科技进步三等奖(1999)、浙江省教委科技进步三等奖(1999)、浙江省科技进步三等奖(2003)各1项,获浙江省高校优秀科研成果一等奖2项(2004、2006)。入选”浙江省151人才工程” (2000),获授权中国发明专利5项,申报发明专利9项。
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徐志康
,-0001,():
-1年11月30日
Increasingly, carbohydrate-protein interactions are viewed as important mechanisms for many biological processes such as blood coagulation, immune response, viral infection, inflammation, embryogenesis, and cellular signal transfer. However, the weak affinity of the interactions and the structural complexity of carbohydrates have hindered efforts to develop a comprehensive understanding of carbohydrate functions. Fortunately, synthetic polyvalent glycoligands give us a chance to reveal the nature of these biological processes. In this work a sugar-containing monomer (R-D-allyl glucoside (AG)) was grafted onto polypropylene microporous membrane (PPMM) by UV-induced graft polymerization to generate a glycosylated porous surface for the first time. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy were employed to confirm the glycosylation. Water contact angle measurement was used to evaluate the hydrophilicity change of the surfaces before and after the graft polymerization of AG. It was found that the grafting density increased reasonably with the increase of AG monomer concentration, and then this increase slowed when the AG concentration exceeded 80 g/L. At the same time a 20-25 min UV irradiation was enough for the grafting polymerization. The photoinitiator concentration also influenced the grafting density obviously, and there was an optimal concentration of the photoinitiator for the grafting process. The water contact angle of the polyAG-tethered membrane surface decreased from 149° to 80° with the increase of grafting density from 0 to 187.76 íg/cm2, which indicated a hydrophilic variation of the membrane surface by the grafting of AG. Results also indicated that the surface-grafted polyAG chains showed weak interaction with Con A when the grafting density was low. However, when the sugar density exceeded 90 íg/cm2, the binding affinity increased dramatically which was the due to the “glycoside cluster effect”.
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徐志康
,-0001,():
-1年11月30日
Carbohydrates play a major role in many recognition events, such as blood coagulation, immune response, fertilization, cell growth, embryogenesis, and cellular signal transfer, which are essential for the survival of living entities. Synthetic carbohydrate-based polymers, so-called glycopolymers, are emerging as important well-defined tools for investigating carbohydrate-based biological processes and for simulating various functions of carbohydrates. In this work, we present a facile strategy for the formation of glycopolymer tethered on polypropylene microporous membrane surface. Acrylamide was grafted onto the polypropylene microporous membrane surface by photoinduced graft polymerization in the presence of benzophenone. The amide groups of grafted poly(acrylamide) were then transformed to primary amine groups by the Hofmann rearrangement reaction. Quantificational evaluation of the rearrangement reaction was carried out by ninhydrin method and mass weighting. Sugar moieties were coupled with the grafted functional layer to form glycopolymer by the reaction between primary amine groups and carbohydrate lactones. The grafting of acrylamide, the conversion of amide groups to amine groups, and the coupling of sugar moieties were confirmed by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy combined with surface morphology observation by scanning electron microscopy.
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徐志康
,-0001,():
-1年11月30日
Carbohydrate residues are found on the extracellular side of the cell membrane. They form a protective coating on the outer surface of the cell and are involved in intercellular recognition. Synthetic carbohydrate-based polymers, so-called glycopolymers, are emerging as important well-defined tools for investigating carbohydrate-based biological processes and for simulating various functions of carbohydrates. In this work, the surface of a polypropylene microporous membrane (PPMM) was modified with comb-like glycopolymer brushes by a combination of UV-induced graft polymerization and surface-initiated atom-transfer radical polymerization (ATRP). 2-Hydroxyethyl methacrylate(HEMA) was first grafted to thePPMMsurface underUVirradiation in the presence of benzophenone and ferric chloride. ATRP initiator was then coupled to the hydroxyl groups of poly(HEMA) brushes. Surface-initiated ATRP of a glycomonomer, D-gluconamidoethyl methacrylate, was followed at ambient temperature in aqueous solvent. Water had a significant acceleration effect on the ATRP process; however, loss of control over the polymerization process was also observed. The addition of CuBr2 to the ATRP system largely increased the controllability at the cost of the polymerization rate. The grafting of HEMA, the coupling of ATRP initiator to the hydroxyl groups, and the surface-initiated ATRP were confirmed by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy.
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【期刊论文】Porphyrinated Nanofibers via Copolymerization and Electrospinning
徐志康
,-0001,():
-1年11月30日
The first example of the synthesis of acrylonitrile copolymers with porphyrin pendants and the subsequent electrospinning of the resultant copolymers into nanofibers is presented in this communication. Vinyl porphyrin monomers have been synthesized and copolymerized with acrylonitrile through solution polymerization. FT-IR, NMR, UV-vis, and fluorescence spectroscopy are used to characterize the copolymers. Preliminary quantum chemical calculations have also been carried out to reveal the activity of the vinyl porphyrin monomers. Nanofibers with a diameter of around 330 nm are prepared by electrospinning the copolymer solutions. Their morphology and porphyrination are clearly observed by fieldemission scanning electron microscopy and fluorescence microscopy. It is speculated that this type of nanofiber may be a latent support of porphyrins for various purposes such as catalysis, molecular imprinting, sensors, and light/energy conversion.
electrospinning, luminescence, nanofiber, polyacrylonitrile, porphyrin
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【期刊论文】Nanofibrous Membranes Containing Carbon Nanotubes: Electrospun for Redox Enzyme Immobilization
徐志康
,-0001,():
-1年11月30日
Nanofibrous membranes that possess reactive groups are fabricated by the electrospinning process from PANCAA solutions that contain MWCNTs. Field emission scanning electron microscopy is used to evaluate the morphology and diameter of the nanofibers. Potentials for applying these nanofibrous membranes to immobilize redox enzymes by covalent bonding are explored. It is envisaged that the electrospun nanofibrous membranes could provide a large specific area and the MWCNTs could donate/accept electrons for the immobilized redox enzymes. Results indicate that, after blending with MWCNTs, the diameter of the PANCAA nanofiber increases slightly. The PANCAA/ MWCNT nanofibrous membranes immobilize more enzymes than that without MWCNTs. Moreover, as the concentration of the MWCNTs increases, the activity of the immobilized catalase is enhanced by about 42%, which is mainly attributed to the promoted electron transfer through charge-transfer complexes and the p system of MWCNTs.
carbon nanotubes, catalase, electrospinning, enzyme immobilization, enzymes, membranes, nanofibrous membranes, poly(, acrylonitrile-co-acrylic acid),
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徐志康
,-0001,():
-1年11月30日
Nanofibrous membranes with an average diameter of 100 and 180nm were fabricated from poly(acrylonitrile-co-maleic acid) (PANCMA) by the electrospinning process. These nanofibrous membranes contain reactive groups which can be used to covalently immobilize biomacromolecules. Two natural macromolecules, chitosan and gelatin, were tethered on these nanofibrous membranes to fabricate dual-layer biomimetic supports for enzyme immobilization in the presence of 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS). Lipase from Candida rugosa was then immobilized on these dual-layer biomimetic supports using glutaraldehyde (GA), and on the nascent PANCMA fibrous membrane using EDC/NHS as coupling agent, respectively. The properties of the immobilized lipases were assayed. It was found that there is an increase of the activity retention of the immobilized lipase on the chitosan-modified nanofibrous membrane (45.671.8%) and on the gelatin-modified one (49.771.8%), compared to that on the nascent one (37.671.8%). The kinetic parameters of the free and immobilized lipases, Km and Vmax, were also assayed. In comparison with the immobilized lipase on the nascent nanofibrous membrane, there is an increase of the Vmax value for the immobilized lipases on the chitosan- and gelatin-modified nanofibrous membranes. Results also indicate that the pH and thermal stabilities of lipases increase upon immobilization. The residual activities of the immobilized lipases are 55% on the chitosan-modified nanofibrous membrane and 60% on the gelatin-modified one, after 10 uses.
Electrospinning, Nanofibrous membranes, Biomacromolecules, Enzyme immobilization, Lipase
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徐志康
,-0001,():
-1年11月30日
Novel nanofibrous membranes containing reactive carboxyl groups were fabricated from poly- (acrylonitrile-co-maleic acid) (PANCMA) by the electrospinning process. The morphology and fiber diameter were analyzed with field emission scanning electron microscopy. It was found that the fiber diameter could be varied from 100 to 600 nm by changing the solution concentration. Lipase from Candida rugosa was covalently immobilized onto the membrane surface via the activation of carboxyl groups in the presence of 1-ethyl-3- ((dimethylamino)propyl)carbodiimide hydrochloride/N-hydroxylsuccinimide. The properties of the immobilized lipases on the nanofibrous and hollow fiber PANCMA membranes were measured. It was found that, compared with the hollow fiber membrane, the enzyme loading and the activity retention of the immobilized lipase on the nanofibrous membrane increase from 2.36 ( 0.06 to 21.2 ( 0.7 mg/g and from 33.9 to 37.6%, respectively. The kinetic constants of the free and immobilized lipases, Km and Vmax, were assayed. Results indicate that the Vmax values are similar for both immobilized enzymes, while the Km value of the immobilized enzyme decreases from 1.36 on the hollow fiber membrane to 0.98 on the nanofibrous membrane. The studied lipase-immobilized nanofibers can be used as biocatalysts for polyester synthesis and/or in situ formation of nanofiber reinforcement composites.
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徐志康
,-0001,():
-1年11月30日
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徐志康
,-0001,():
-1年11月30日
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徐志康
,-0001,():
-1年11月30日
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