徐静娟
纳米生物电化学,生物分子电子器件,微流控芯片-电化学检测。
个性化签名
- 姓名:徐静娟
- 目前身份:
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- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
分析化学
- 研究兴趣:纳米生物电化学,生物分子电子器件,微流控芯片-电化学检测。
徐静娟,1968年10月生。1990年毕业于武汉大学化学系;1997、2000年在南京大学分别获得硕士和博士学位。 2000.08 留南京大学化学系任教,2003年11月聘为副教授,2006年6月聘为教授,2007年4月为博士生导师,分析化学学科副主任。2007年入选教育部“新世纪优秀人才支持计划”。2007/2008年度获唐氏基金资助(唐氏基金康奈尔-中国学者项目)赴美国Cornell University进行为期1年的合作科研。
已发表SCI论文90余篇,被SCI刊物他人引用1000余次;申请专利6项。作为项目负责人先后主持国家自然科学基金面上项目3项,教育部重点科学技术项目1项,教育部新世纪优秀人才项目1项。2007年获国家自然科学奖二等奖1项(排名第二),2006年和 2001年分别获教育部自然科学奖一等奖和中国高校科学技术奖一等奖各1项。
研究方向:纳米生物电化学,生物分子电子器件,微流控芯片-电化学检测。
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253
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成果数
7
【期刊论文】辣根过氧化物酶在MnO2纳米片薄膜中的直接电化学与电催化行为
徐静娟, 肖寒, 吴金玲, 陈旭, 杨文胜*
科学通报,,2007,52(19):2255~2259,-0001,():
-1年11月30日
采用新型纳米材料MnO2纳米片作为固定辣根过氧化物酶(HRP)的载体,制备了HRP/MnO2纳米片修饰的玻碳电极(GCE)。在MnO2纳米片薄膜中,HRP能够实现有效的直接电子转移,在pH6.5的磷酸缓冲溶液中,修饰电极的循环伏安曲线上显示出一对可逆的氧化还原峰,式量电位为−0.315V(vs.Ag/AgCl)。HRP在MnO2纳米片/GCE表面的电子转移速率常数为6.86s−1。HRP/MnO2纳米片/GCE的式量电位(mV)在pH 4.0~8.0范围内与溶液pH值成线性关系,其斜率为−53.75mV·pH−1,表明电极反应在发生一个电子转移的同时还伴随一个质子的转移。修饰后的电极对H2O2有良好响应,响应时间小于3s,在信噪比为3时,最低检出限为0.21μmol·L−1。
辣根过氧化物酶(, HRP), MnO2 纳米片 直接电化学 H2O2
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徐静娟, Wei Zhao, † Si-Xin Sun, ‡ Jing-Juan Xu, *, † Hong-Yuan Chen, † Xiao-Jian Cao, ‡ and Xiao-Hong Guan§
Anal. Chem. 2008, 80, 3769-3776,-0001,():
-1年11月30日
We report a simple electrochemical method for the identification of properties of peripheral nerve fibers, based on the detection of a neurotransmitter enzyme, acetylcholinesterase (AChE). A poly(diallydimethylammonium) (PDDA) adulterated poly(dimethylsiloxane) (PDMS) film is spin-coated on the surface of gold electrodes. Gold nanoparticles (AuNPs) are in situ synthesized on the polymer film, which act as "electron antennae" between the film and the electrode surface and also provide a biocompatible interface. This PDMS-PDDA/AuNPs film shows different adsorption sites to choline oxidase (ChO) and AChE; after incubation with ChO, the polymer-gold nanocomposite film also shows excellent adsorption ability to AChE. Moreover the adsorption sites of AChE would not be blocked by bovine serum albumin (BSA) which provides a good platform for the quantitative amperometric determination of AChE via the oxidation of the enzymatically generated H2O2 in the bienzyme system in the presence of acetylcholine. The detection limit is down to 1.0unit/mL. The polymer-gold anocomposite film shows excellent anti-interference ability to the coexistent electroactive substances such as ascorbic acid. Thus it was applied to determine AChE in peripheral nerve fibers homogenates and identify the motor and sensory fibers for the first time. Compared with histochemical staining methods, the electrochemical technique shows good accurate rate and faster response, which has good potential for a clinical application.
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徐静娟, Wei Zhao, Jing-Juan Xu, * Chuan-Guo Shi, and Hong-Yuan Chen
Langmuir 2005, 21, 9630-9634,-0001,():
-1年11月30日
Polyelectrolyte multilayers (PEMs) are now widely used for bioanalytical applications. In this work, a bilayer of poly(diallydimethylammonium chloride) (PDDA) and poly(sodium 4-styrenesulfonate) (PSS) is consecutively adsorbed on 3-mercapto-1-propanesulfonic acid modified Au electrode surfaces, forming stable, ultrathin multilayer films. Subsequently, Prussian blue nanoparticles protected byPDDA(denoted as P-PB) and negatively charged glucose oxidase (GOx) are consecutively adsorbed onto the PSS-terminated bilayer. The growth of each of the P-PB/GOx bilayers is followed quantitatively usingUV-visible absorption spectroscopy and the electrochemical method. The P-PB nanoparticles can catalyze the electroreduction of hydrogen peroxide formed from enzymatic reaction at lower potential and inhibit the responses of interferents, such as ascorbic acid (AA) and uric acid (UA). Performance of the multilayer films can be tailored by controlling the number of bilayers. Under optimal conditions, a linear range of 0.10 to 11.0mM and a detection limit of 10 íMwere achieved. The glucose biosensor has good stability and reproducibility.
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徐静娟, Yu-Hui Bai, Ying Du, Jing-Juan Xu*, Hong-Yuan Chen
Electrochemistry Communications 9(2007)2611-2616,-0001,():
-1年11月30日
An interesting mode of reactivity of MnO2 nanoparticles modified electrode in the presence of H2O2 is reported. The MnO2 nanoparticles modified electrodes show a bi-direction electrocatalytic ability toward the reduction/oxidation of H2O2. Based on this property, a choline biosensor was fabricated via a direct and facile electrochemical deposition of a biocomposite that was made of chitosan hydrogel, choline oxidase (ChOx) and MnO2 nanoparticles onto a glassy carbon (GC) electrode. The biocomposite is homogeneous and easily prepared and provides a shelter for the enzyme to retain its bioactivity. The results of square wave voltammetry showed that the electrocatalytic reduction currents increased linearly with the increase of choline chloride concentration in the range of 1.0×10 -5 -2.1×10 -3 M and no obvious interference from ascorbic acid and uric acid was observed. Good reproducibility and stability were obtained. A possible reaction mechanism was proposed.
Biosensor, Choline, Choline oxidase, MnO2 nanoparticles, Bi-direction electrocatalysis
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【期刊论文】Nanocrystalline diamond modified gold electrode for glucose biosensing
徐静娟, Wei Zhao, Jing-Juan Xu*, Qing-Qing Qiu, Hong-Yuan Chen
Biosensors and Bioelectronics 22(2006)649-655,-0001,():
-1年11月30日
Boron-doped diamond has drawn much attention in electrochemical sensors. However there are few reports on non-doped diamond because of its weak conductivity. Here, we reported a glucose biosensor based on electrochemical pretreatment of non-doped nanocrystalline diamond (N-NCD) modified gold electrode for the selective detection of glucose. N-NCD was coated on gold electrode and glucose oxidase (GOx) was immobilized onto the surfaces of N-NCD by forming amide linkages between enzyme amine residues and carboxylic acid groups on N-NCD. The anodic pretreatment of N-NCD modified electrode not only promoted the electron transfer rate in the N-NCD thin film, but also resulted in a dramatic improvement in the reduction of the dissolved oxygen. This performance could be used to detect glucose at negative potential through monitoring the current change of oxygen reduction. The biosensor effectively performs a selective electrochemical analysis of glucose in the presence of common interferents, such as ascorbic acid (AA), acetaminophen (AP) and uric acid (UA). A wide linear calibration range from 10 M to 15mM and a low detection limit of 5 M were achieved for the detection of glucose.
Non-doped nanocrystalline diamond, Electrochemical pretreatment, Glucose oxidase, Biosensor
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【期刊论文】Fabrication, characterization and application of gold nano-structured film
徐静娟, Wei Zhao, Jing-Juan Xu*, Chuan-Guo Shi, Hong-Yuan Chen
Electrochemistry Communications 8(2006)773-778,-0001,():
-1年11月30日
A straightforward, rapid and "green" approach was proposed for the fabrication of gold nano- tructured films. A gold electrode was firstly oxidized under a high potential of 10 V, and β-D-lucose, as a nontoxic reducing agent, was then chosen to reduce the gold oxide. This process gives a fractal gold nanofilm on the top of the gold electrode in a way of completely "green" chemistry. X-ray photoelectron spectroscopy, atomic force microscopy, scanning electron microscopy and electrochemical methods were adopted to characterize the asprepared gold nanofilm. The gold nanofilm was found to have abundant adatom state Au*, evidenced by the electrochemical process of Au*/Au*OHads couple. The as-prepared gold nanofilm greatly facilitates the electrochemical oxidation of glucose in neutral solutions. As the gold nanofilm was formed in situ on the gold electrode, additional immobilization procedures are not necessary. A sensitive enzymefree sensor can be easily developed for the detection of glucose in pH 7.4 phosphate buffer solution, which gives linear ranges up to 57.5 and 30mM with detection limits down to 0.75 and 3.6lM at potential of +0.3 and 0.15 V, respectively. A rapid response for glucose could be achieved in less than 2 s. At a detection potential of 0.15 V, physiological interference agents such as ascorbic acid, uric acid and acetylaminophenol were efficiently excluded.
Gold nano-structured film, β-D-glucose, Enzyme-free sensor, Glucose sensor
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徐静娟, Jing-Juan Xu, Ying Peng, Ning Bao, Xing-Hua Xia, Hong-Yuan Chen*
Journal of Chromatography A, 1095(2005)193-196,-0001,():
-1年11月30日
Detection of native amino acidswas accomplished using a capillary electrophoresis (CE)-amperometric detection system, in which a single carbon fiber cylinder (CFC) working electrode instead of a carbon fiber disc (CFD) electrode was mounted in the end part of a poly(dimethylsiloxane) (PDMS)/glass hybrid microchannel. Similar to that using CFD working electrode, here, the electrochemical reduction reaction at the working electrode is also coupled from the separation high voltage system, the coupling degree is related to the in-channel length of the CFC. This property simplifies the fabrication procedure of the working electrode and also provides a convenient and sensitive means for the determination of nonelectroactive ions by amperometry. The present detection mode is successfully used to electrochemically detect non-electroactive arginine (Arg), threonine (Thr), glutamic acid (Glu) and electroactive cysteine (Cys). Furthermore, by simply changing the detection potential, we can easily distinguish peak mobilities of electroactive amino acids from that of non-electroactive amino acids.
Indirect amperometric detection, Microchip electrophoresis, Amino acids
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