何兴金
植物分类学、植物系统与分子进化、资源植物保护与利用、自然科技资源平台建设、天然产物开发、生物安全与外来入侵植物等研究。
个性化签名
- 姓名:何兴金
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学术头衔:
博士生导师
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学科领域:
植物学
- 研究兴趣:植物分类学、植物系统与分子进化、资源植物保护与利用、自然科技资源平台建设、天然产物开发、生物安全与外来入侵植物等研究。
何兴金,1985年7月毕业于西华师范大学(原南充师范学院)生物系,1991年7月中国科学院成都生物研究所获理学硕士,1999年7月四川大学生命科学学院获理学博士,1999年9月-2001年8月中山大学生命科学学院作博士后研究,2002年4月担任中国科学院成都生物研究所植物学专业兼职博士生导师,2003年7月至今在四川大学生命科学学院工作,任教授、博士生导师。
现任四川大学自然博物馆馆长,四川省植物学会理事(植物分类学专业主任),SCI期刊《Journal of Systematics and Evolution》编委,四川省学术和技术带头人后备人选。
研究方向:植物分类学、植物系统与分子进化、资源植物保护与利用、自然科技资源平台建设、天然产物开发、生物安全与外来入侵植物等研究。
研究内容:在伞形科、百合科等科进行了较为深入的分类学研究,尤以对中国独活属、葱属、百合族、柴胡属、大戟属等进行了较全面而深入的细胞分类学 、分子系统学和地理学研究;对部分药用植物独活、当归、柴胡的DNA barcoding进行了研究;同时对四川分布的种子植物区系和物种现状及资源植物利用与保扩进行了较为深入的野外考察和分类鉴定、保护和开发研究工作。对资源植物有效成分利用的关键技术方面进行了研究工作。对植物标本数据库与信息系统和共享平台进行了系统的研究,对四川高等植物进行了物种数据库和经济植物的GIS系统建设研究,对多个四川省内的自然保护区进行生物多样性调查,外来入侵物种及其防治研究等。
主持和参加科研项目28项,其中科技部专项3项(项目并列负责人和子课题负责人),国家重点项目4项,在研究项目4项。已在国内外重要学术刊物上发表论文90余篇,其中SCI期刊及SCI检索论文16篇。
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何兴金, 何川生, , 葛颂, 李天飞, 许凌云, 许美玲, 许介眉
植物学报,2001,43(6):610~614,-0001,():
-1年11月30日
从156个随机引物中筛选出30个引物,对来自国内外的31个烤烟品种进行了遗传多样性和亲缘关系的RAPD分析。在检测的246个位点中,127个位点为多态位点(52%)。聚类分析表明,不同烤烟品种之司存在明显差异,31个品种基本上可分为4大类,品种最多的第一大类(19个)主要由来自美国的Orinoco烤烟选育而成,反映了我国现在推广的烤烟品种遗传基础比较狭窄。研究结果表明,RAPD技术可用于烤烟品种的鉴别和纯度测定。研究为烤烟杂种育种中亲本的选配提供了一定的理论依据。
烤烟, 品种资源, RAPD分析
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何兴金, 周颂东, 何兴金*, 余岩, 许介眉
植物分类学报,2007,45(2):207~216,-0001,():
-1年11月30日
对葱属根茎组Allium sect. Rhiziridium的8种21个地方居群的核型进行研究,以期为解决该组的种间亲缘关系和物种进化机制提供依据。贺兰韭A. eduardii和阿拉善韭A. flavovirens 2个种的核型以及辉韭A. strictum的六倍体核型均属首次报道。研究结果表明:贺兰韭A. eduardii、阿拉善韭A. flavovirens、北韭A. lineare、蒙古韭A. mongolicum和滩地韭A. oreoprasum的各居群均为二倍体,核型类型为Stebbins的2A型;韭A. tuberosum和野韭A. ramosum的各个居群均为四倍体,核型类型为2A型;辉韭A. strictum的4个居群均为六倍体,核型类型为2B型。通过研究可以得出如下推论:(1)该组植物中存在着大量的多倍体或多倍体系列,染色体数目变化与物种进化具有密切相关性,多倍化可能是根茎组植物核型进化的重要机制之一;(2)随体染色体多为st或t染色体,均位于短臂末端;(3)可以认为辉韭是以增加倍性来克服该物种扩大新的生存空间所带来的困难;(4)现今栽培的韭可能是由野生的二倍体韭和四倍体韭经过长期人工驯化而来的,现今栽培的三倍体韭可能是二倍体韭和四倍体韭杂交而来,并且以无性繁殖方式保存三倍体类群的存在。
葱属, 根茎组, 核型, 多倍体, 种内分化, 进化
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何兴金, 薛恒钢, 周颂东, 何兴金*, 余岩
植物分类学报,2007,45(5):619~626,-0001,():
-1年11月30日
对国产大戟科Euphorbiaceae大戟属Euphorbia的13种15个地方居群的核型进行研究,以期为解决该属的种间亲缘关系和物种进化机制提供依据。结果如下:地锦E-humifusa (望江居群),2n=2x=22=20m(2SAT)+2sm;地锦E-humifusa (红旗村居群),2n=2x=22=18m+4sm;千根草E-thymifolia,2n=4x=40=40m;斑地锦E-maculata,2n=4x=40=36m+4sm;银边翠E-marginata,2n=8x=56=40m+ 8sm+8st;铁海棠E-milii,2n=4x=40=32m+8sm;金刚纂E-neriifolia,2n=6x=60=6m+36sm+12st+6t;火殃勒E-antiquorum,2n=6x=60=24m+24sm+12st;一品红E-pulcherrima,2n=4x=28=24m(3SAT)+4sm;猩猩草E-cyathophora,2n=4x=28=16m+12sm;齿裂大戟E-dentata,2n=4x=28=28m;续随子E-lathyris,2n=2x=20=12m+8sm;泽漆E-helioscopia,2n=6x=42=36m+6sm;乳浆大戟E-esula (陈家沟居群),2n=2x=20=14m+6sm;乳浆大戟E-esula (樱桃沟居群),2n=4x=40=32m+8sm。千根草和斑地锦两个种的核型以及乳浆大戟的四倍体居群核型均为首次报道。
大戟属, 大戟科, 核型, 多倍体, 细胞分类
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何兴金, Qi-Zhi WANG* Xing-Jin HE** Song-Dong ZHOU* Yun-Ke WU Yan YU Yun-Li PANG
Journal of Systematics and Evolution 46(2): 142-154(2008),-0001,():
-1年11月30日
Hengduan Mountains are the diversity center for the genus Bupleurum of Apiaceae in China. This paper reports chromosome numbers of six species and two varieties of Bupleurum, and for four species and two varieties their chromosome numbers are reported for the first time. The phylogeny of Bupleurum was investigated based on the ITS region of the nuclear ribosomal DNA (nrDNA) of 14 taxa from the Hengduan Mountains, 3 taxa from the North China (Hebei and Heilongjiang), and 16 taxa from Africa and the Mediterranean region. Variations in chromosome numbers and the ITS sequences were used to infer phylogenetic relationships between Bupleurum species in Hengduan Mountains. The results showed that the Hengduan Mountains might represent one of the frequency and diversity centers for Bupleurum. The ancestors of Bupleurum species in the Hengduan Mountains may be related to the woody B. fruticosum in North Africa, or the species in the western Mediterranean region. It is postulated that the ancestral population migrated into Hengduan Mountains through the Middle East and the Caucasus. Furthermore, the neo-endemic B. mundtii in South Africa appeared to be a close relative of the species in the Hengduan Mountains. In the trend of basic chromosome number evolution, x=8 should be regarded as the ancestral basic number, while x= 6, 7 as the derived ones. The Bupleurum species in the Hengduan Mountains have been undergoing changes in the basic chromosome numbers or the ploidy level. The ITS phylogenetic tree showed that the Chinese species were divided into two clades: one with the basic chromosome number x = 8, and the other with x=6, 7. The results rejected the previous infrageneric classification of Bupleurum in China. We further suggested to raise B. marginatum var. stenophyllum to species rank based on the combined evidence from morphology, karyology, pollen morphology, and the ITS phylogenetic tree.
Bupleurum,, chromosome,, Hengduan Mountains,, ITS,, phylogeny,, taxonomy.,
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何兴金, QI-ZHI WANG*, SONG-DONG ZHOU, TING-YU LIU, YUN-LI PANG, YUN-KE WU AND XING-JIN HE**
ACTA BIOLOGICA CRACOVIENSIA Series Botanica 50/2: 105-116, 2008,-0001,():
-1年11月30日
Separate and combined analyses of nuclear ribosomal DNA internal transcribed spacer (ITS) and cpDNA rps16 provided phylogenetic hypotheses and molecular data for the taxonomy of Chinese Bupleurum species. The phylogenetic results derived from Bayesian and maximum parsimony analyses supported the monophyly of the Bupleurum with strong evidence. The origin of Chinese Bupleurum is likely to be through the Middle East and the Caucasus from the Mediterranean region with the basic chromosome number (8→7→6), and polyploidization. Our molecular data are not consistent with other earlier Chinese Bupleurum classifications and are consistent with the molecular classification proposed by Neves and Watson. The analyses also provide molecular data to elucidate the taxonomic treatments for Bupleurum falcatum from China and Europe.
Chinese Bupleurum,, phylogeny,, taxonomy,, ITS,, rps16.,
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【期刊论文】Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum
何兴金, Yijun Fana, c, XingJin Hea, ∗, Songdong Zhoua, Aoxue Luoa, Tao Heb, Ze Chunb
International Journal of Biological Macromolecules 45(2009)169-173,-0001,():
-1年11月30日
Three polysaccharide fractions (DDP1-1, DDP2-1 and DDP3-1) were successfully purified from the crude polysaccharide of Dendrobium denneanum by DEAE-Cellulose and Sephadex G-200 column chromatography. The average molecular weights (Mws) of these fractions were 51.5, 26.1 and 6.95 kDa, respectively. Monosaccharide components analysis indicated that DDP1-1 and DDP2-1 were composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.82:57.11:140.82:7.76 and 1.00:1.62:1.18:77.5:7.79. DDP3-1 was composed of arabinose, mannose, glucose and galactose in a molar ratio of 1.00:1.03:8.84:2.00. On the basis of antioxidant test in vitro, DDP2-1 exhibited the highest antioxidant ability among these samples.
Dendrobium denneanum Polysaccharide Antioxidant activity Composition Free radicals
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【期刊论文】Karyotype and cytogeography of the genus Heracleum (Apiaceae) in the Hengduan Mountains
何兴金, Xian-Lan DENG Xing-Jin HE∗ Wei-Lue HE Yun-Dong GAO Hai-Yan LIU Yu-Cheng ZHANG
Journal of Systematics and Evolution 47(4): 1-14(2009),-0001,():
-1年11月30日
In the present study, the karyotypes of 34 populations belonging to 11 species and one variety of Heracleum from the Hengduan Mountains in China were examined. Chromosome numbers and the karyotypes of three species (H. souliei, H. kingdoni, and H.wenchuanense) are reported for the first time, as are the karyotypes of H. moellendorffii and H. henryi (tetraploid). Populations of H. candicans, H. franchetii, and H. kingdoni in the Hengduan Mountains were found to consist of a mixture of diploid and tetraploid plants. Except for four species of Heracleum, namely H. candicans, H. franchetii, H. henryi, and H. kingdoni, which have both diploid and tetraploid karyotypes, all other species of Heracleum are were found to be diploid. All karyotypes were found to belong to the 2A type of Stebbins, with the exception of H. candicans var. obtusifolium, which belongs to 2B, and H. hemsleyanum and H. franchetii (Mt Dujuan, Daocheng, Sichuan, China), which belong to 1A. There was only a slight difference in the karyotype asymmetry index, which suggests a close kinship for species of Heracleum and that the entire phylogenetic development of Heracleum is relatively primitive. Species that exhibited advanced morphological features were also more advanced in karyotype structure, with the order of karyotype evolution being 1A→2A→2B. This phenomenon indicates that the species distributed in the Hengduan Mountains have not diverged completely and that the Hengduan Mountains are a relatively young and active area for the evolution of Heracleum. Polyploidization in Heracleum may be an important evolutionary mechanisms for some species, generating diversity. The biological attributes, distribution range, and the geological history of the genus have all played a part in accelerating the evolution through polyploidization or aneuploidization. It is known that as the distribution latitude of Heracleum decreases from north to south, the chromosome number, ploidy level, and asymmetry structure appear to increase. In the Hengduan Mountains, these tendencies are also evident. Finally, based on all the available cytogeographic data, we speculate that the more advanced tetraplont or aneuploid species of Heracleum in India may be derived from early diplont species that were distributed in the Caucasus region and Hengduan Mountains. The dispersal of Heracleum was from Eurasia to India, because this correlates with the emergence of the Himalayan Mountains through tectonic movement. Thus, the Hengduan Mountains are not only a center of diversity for Heracleum, but also a center of active speciation in modern times.
cytogeography,, evolution,, Heracleum,, karyotype,, polyploidization.,
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何兴金, AoXue Luoa, c, XingJin Hea, ∗, SongDong Zhoua, YiJun Fana, Tao Heb, Ze Chunb
International Journal of Biological Macromolecules 45(2009)359-363,-0001,():
-1年11月30日
A water-soluble polysaccharide (DNP), isolated from the aqueous extracts of the stem of Dendrobium nobile Lindl., was found to have an average molecular weight (Mw) of about 8.76×104 Da. Monosaccharides analysis revealed that DNP was composed of rhamnose, arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.80:2.20:30.76:117.96:31.76. The evaluation of antioxidant activity in vitro revealed that DNP is a novel potential antioxidant. The NMR spectra suggested that the main structure of DNP was possible as
Dendrobium nobile Lindl., Polysaccharide Monosaccharides composition Structure Free radicals Antioxidant activities
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何兴金, AoXue Luo a, c, , XingJin He a, *, SongDong Zhou a, YiJun Fan a, AoShuang Luo b, Ze Chun b
Carbohydrate Polymers 79(2010)1014-1019,-0001,():
-1年11月30日
Crude water-soluble polysaccharides were extracted from the stem of Dendrobium nobile Lindl. using boiling-water. The polysaccharides were successively purified by chromatography on DEAE-cellulose and Sephadex G-200 column, giving four major polysaccharide fractions termed DNP1-1, DNP2-1, DNP3-1, DNP4-2. The gel permeation chromatography (GPC) analysis showed that the average molecular weight (Mw) of polysaccharides (DNP1-1, DNP2-1, DNP3-1, DNP4-2) were approximately 136 kDa, 27.7 kDa, 11.8 kDa and 11.4 kDa, respectively. Monosaccharides analysis revealed the dominance of mannose, glucose, galactose, and smaller amounts of rhamnose, arabinose and xylose in the four polysaccharides. The evaluation of antioxidant activity suggested that DNP4-2 had good potential for scavenging activity of ABTS radical, and higher scavenging activity of hydroxyl radical and DPPH radical than the other polysaccharides, and should be explored as a novel potential antioxidant.
Dendrobium nobile Lindl., Polysaccharide Monosaccharides composition Antioxidant activity
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何兴金, Yan Yua, A.J. Harrisb, Xingjin Hea, *
Molecular Phylogenetics and Evolution 56(2010)848-850,-0001,():
-1年11月30日
Dispersal-Vicariance Analysis (DIVA) is one of the most widely used methods of inferring biogeographic histories. Here we present a simple tool that complements DIVA and uses a Statistical Dispersal-Vicariance Analysis (S-DIVA) to statistically evaluate the alternative ancestral ranges at each node in a tree accounting for phylogenetic uncertainty and uncertainty in DIVA optimization. S-DIVA provides a point-and-click user interface and displays results as, high-resolution, exportable graphics. S-DIVA is freely available for download for Windows at http://mnh.scu.edu.cn/S-DIVA.
DIVA Biogeographic Program Phylogeny Statistics
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