刘建辉
研究方向为神经退行性疾病的发病机制及脑保护,熟悉中药神经活性成分的高通量筛选及药理学机制研究、细胞培养、免疫组化、细胞信号转导、酶学活性测定及分子生物学等相关技术。
个性化签名
- 姓名:刘建辉
- 目前身份:
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- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
药物化学
- 研究兴趣:研究方向为神经退行性疾病的发病机制及脑保护,熟悉中药神经活性成分的高通量筛选及药理学机制研究、细胞培养、免疫组化、细胞信号转导、酶学活性测定及分子生物学等相关技术。
刘建辉:男,博士,教授,2003年从大连理工大学生物工程系博士毕业,毕业后到复旦大学医学神经生物学国家重点实验室工作,2004年被重庆工商大学作为学术带头人引进,聘为教授。目前担任重庆工商大学药物化学与化学生物学研究中心副主任。
研究方向为神经退行性疾病的发病机制及脑保护,熟悉中药神经活性成分的高通量筛选及药理学机制研究、细胞培养、免疫组化、细胞信号转导、酶学活性测定及分子生物学等相关技术。参与或完成了包括国家“973”计划、国家自然科学基金重点和面上以及地方资助的科研项目多项,近三年在国内外期刊上发表15篇文章,其中SCI期刊12篇,申请国家发明专利2项,获得国家实用新型专利1项。
目前正在做的课题及其简介:
国家自然科学基金项目“京尼平苷激活GLP-1受体的抗氧化作用机制研究”(34万)、重庆市科委重大攻关项目“Klotho启动子激动剂的功能及作用机制研究”(150万),重庆市科委重点基金项目“三乙烯四胺抑制端粒酶的作用机制及应用研究”(30万),重庆市教委项目“抗肿瘤新药TETA活性研究”(4.5万))等。
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18
刘建辉, Jianhui Liu a, Fei Yin a, Xuxu Zheng a, Jiajia Jing a, Yinhe Hu b
Neurochemistry International 51(2007)361-369,-0001,():
-1年11月30日
Alzheimer's disease (AD) is the most common form of dementia. Glucagon-like peptide-1 (GLP-1) gives a new genre in therapeutic targets for intervention in AD with its neurotrophic and neuroprotective functions. In previous work, we identified that geniposide is a novel agonist for GLP-1 receptor, which shows neurotrophic characteristics to induce the neuronal differentiation of PC12 cells. The aim of this study is to determine whether geniposide prevents neurons from oxidative damage, and to explore its signaling pathways. The results demonstrated that geniposide increased the expression of anti-apoptotic proteins, including Bcl-2 and heme oxygenase-1 (HO-1), to antagonize the oxidative damage in PC12 cells induced by hydrogen peroxide. LY294002 (a PI3K inhibitor) inhibited the effect of geniposide increasing of Bcl-2 level by activation of MAPK, MEK and c-Raf phosphorylation in hydrogen peroxide treated PC12 cells. U0126 (a selective inhibitor of MEK) also attenuated the enhancement of geniposide on Bcl-2 level by inhibiting the phosphorylation of p90RSK in the hydrogen peroxide treated PC12 cells. All these data demonstrate that geniposide, an agonist for GLP-1 receptor, regulates expression of anti-oxidative proteins including HO-1 and Bcl-2 by activating the transcriptor of p90RSK via MAPK signaling pathway in PC12 cells.
Geniposide, Glucagon-like peptide-1 receptor, Oxidative stress, Mitogen-activated protein kinase
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【期刊论文】Neurotrophic property of geniposide for inducing the neuronal differentiation of PC12 cells
刘建辉, Jianhui Liu a, Xuxu Zheng a, Fei Yin a, Yinhe Hu b, Lixia Guo a, Xiaohong Deng a, Gang Chen a, Jing Jiajia a, b, Heng Zhang a
Int. J. Devl Neuroscience 24(2006)419-424,-0001,():
-1年11月30日
The emerging data show that the insulinotrophic hormone glucagon-like peptide-1(GLP-1) and its agonist extendin-4 have neurotrophic function to inducing neuronal differentiation of PC12 cells and prevent neurons damage challenged by oxidative stress. Here, with the model of high throughput screen for GLP-1 receptor agonists, we screen and identify that geniposide is a novel agonist for GLP-1 receptor. Furthermore, geniposide induces the neuronal differentiation of PC12 cells with resulting neurites outgrowth; we also observe an increase in expression of growth-associated protein-43. U0126, a selective MEK inhibitor, prevents neurites out growth and phosphorylation of mitogen-activated kinase proteins in PC12 cells induced by geniposide. All these results show that activation of GLP-1 receptor by geniposide to induce the neuronal differentiation of PC12 cells involves in MAPK signaling cascade.
Glucagon-like peptide-1, Geniposide, Differentiation, Neurite, Mitogen-activated protein kinase
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【期刊论文】Characterization and antitumor activity of triethylene tetramine, a novel telomerase inhibitor
刘建辉, Jianhui Liu, Lixia Guo, Fei Yin, Xuxu Zheng, Gang Chen, Yun Wang
Biomedicine & Pharmacotherapy 62(2008)480-485,-0001,():
-1年11月30日
Triethylene tetramine (TETA) is a novel ligand for G-quadruplex and has been reported to have many kinds of biological activities, including telomerase inhibition, inducing the senescence of tumor cells, etc. In this study, tumor inhibiting activity of TETA and its mechanism were investigated in HeLa cells, MCF-7 cells and mice transplanted with S180 tumor. Results indicated that TETA inhibited telomerase activity involved in decreasing the expression of human telomerase reverse transcriptase (hTERT). We also observed that a low concentration of TETA had limited ability to inhibit the growth of tumor cells in short-term culture, but it could significantly enhance antitumor activity of traditional antitumor drugs in vitro and in vivo.
Triethylene tetramine, Telomerase, Human telomerase reverse trans, c, r, i, p, t, ase (, hTERT),
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刘建辉, Shuzhen Kong, Jian-Hui Liu, Andrew G. Ramage and YunWang,
Exp Physiol 92.6 pp 1023-1028,-0001,():
-1年11月30日
Cardiac vagal preganglionic neurones (CVPNs) show respiratory modulation in the nucleus ambiguus but not in the dorsal vagal nucleus. Both types of neurones can be activated by pulmonary C fibre afferents. Another brainstem area that has been identified as containing CVPNs is the intermediate zone between the dorsal vagal nucleus and nucleus ambiguus. Experiments were carried out in α-chloralose-anaesthetized cats to determine the physiological properties of these CVPNs and their responses to pulmonary C fibre afferent activation. Seven CVPN axons in the right cardiac vagal branches were identified and found to be localized in the intermediate zone with a conduction velocity of between 1.2 and 1.6ms−1, in the C fibre range. These seven CVPNs [either showing spontaneous activity (n=1) or having activity induced by DL-homocysteic acid applied ionophoretically (n=3)] were neither respiratory modulated nor did they receive a baroreceptor input, thus being similar to those found in the dorsal vagal nucleus. Right atrial injections of phenylbiguanide excited all four CVPNs tested. In conclusion, CVPNs located in the intermediate zone have similar properties to those in the dorsal vagal nucleus but not the nucleus ambiguus.
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刘建辉, Liu Jian-Hui, Jiang Bo, Bao Yong-Ming, An Li-Jia
Int. J. Devl Neuroscience 21(2003)277-281,-0001,():
-1年11月30日
Exposure of rat pheochromocytoma PC12 cells to Cuscuta chinensis glycoside induced neuronal differentiation with resulting outgrowth of neurites and increase of acetylcholinesterase activity. A specific inhibitor of mitogen-activated protein kinase (MAPK) kinase, PD98059, prevented this effect of C. chinensis on PC12 cells. These results suggested that C. chinensis glycoside induced neuronal differentiation in PC12 cells linked to the mitogen-activated protein kinase signaling cascade.
Acetylcholinesterase, Differentiation, Cuscuta chinensis, Mitogen-activated protein kinase, Neurite
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刘建辉, Jian Hui LIU, Fei YIN, and Xu Xu ZHENG
Biol. Pharm. Bull. 28(4)768-771(2005),-0001,():
-1年11月30日
Rat pheochromocytoma PC12 cells undergo neuronal differentiation in response to nerve growth factor. We show here that exposure of PC12 cells to Nardostachys chinensis glycoside induces the outgrowth of neurites, increases the activity of AChE, triggers cell cycle arrest in G1 and enhances the expression of growth associated protein 43 (GAP-43). Both the outgrowth of neurites and the increase in AChE activity are prevented partly by PD98059, a specific inhibitor of MEK1. These results suggest that N. chinensis glycoside induces the characteristics of neuronal differentiation in PC12 cells via the mitogen-activated protein kinase (MAPK)-related signal cascade.
Nardostachys chinensis glycoside, acetylcholinesterase, mitogen-activated protein kinase, neurite
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【期刊论文】Triethylene Tetraamine: A Novel Telomerase Inhibitor
刘建辉, Fei Yin a, Jianhui Liu b, and Xiaojun Peng a
Bioorganic & Medicinal Chemistry Letters 13(2003)3923-3926,-0001,():
-1年11月30日
Telomerase inhibition can be achieved by stabilization of G-quadruplex structure. Triethylene tetraamine, a small linear molecule, has been identified as a potent telomerase inhibitor. It stabilizes both intra-and inter-molecular G-quadruplexes and shows a good differential between potent telomerase inhibition and acute cytotoxicity.
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刘建辉, LIU Jian-Hui, BAO Yong-Ming, SONG Ji-Jun, AN Li-Jia
Acta Pharmacol Sin 2003 Sep; 24 (9): 913-917,-0001,():
-1年11月30日
To explore the effect of Codonopsis pilosula (Franch) Nannf total alkaloids (DSA) on differentiation induced by nerve growth factor (NGF) in PC12 cells. METHODS: After culturing PC12 cells with DSA in the presence or absence of NGF, neurite outgrowth in PC12 cells and correlated protein kinases were assayed. RESULTS: DSA alone did not exhibit neuritogenic activity, but caused a significant enhancement of NGF (2 μg/L)-induced neurite outgrowth in PC12 cells, and increased the phosphorylation of mitogen-activated protein kinase (MAPK). Furthermore, this enhancing effect was completely blocked by a specific MAPK kinase inhibitor, PD98059. CONCLUSION: DSA enhanced the NGF-induced neurite outgrowth in PC12 cells by amplifying an up-stream step of the MAPK-dependent signaling pathway.
Codonopsis, alkaloids, mitogen-activated protein kinases, nerve growth factors, neurites
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刘建辉
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-1年11月30日
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【期刊论文】Neurotrophic property of geniposide for inducing the neuronal differentiation of PC12 cells
刘建辉
,-0001,():
-1年11月30日
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