史林启
嵌段共聚物的自组装与化学生物传感器
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- 姓名:史林启
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学术头衔:
博士生导师
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学科领域:
高分子化学
- 研究兴趣:嵌段共聚物的自组装与化学生物传感器
史林启,男,1963年9月生,1984年河北大学化学系毕业,国科学院长春应用化学研究所获博士学位,19993年南开大学博士后,现为南开大学高分子化学研究所教授,博士生导师,2003年11月-2004年2月加拿大蒙特利尔大学访问教授,2004年3月-2004年5月美国纽约州立大学石溪分校访问教授,主要研究方向为嵌段共聚物的自组装与化学生物传感器的研究,相关的研究结果分别发表在Macromolecules,J. Phys. Chem. B和Langmuir等国际著名杂志,共发表研究论文70余篇,SCI收录论文22篇,先后主持国家自然科学基金4项,作为中方负责人主持海外青年学者合作基金1项和教育部重点项目等,参与编写专著2部,安全玻璃膜片和安全玻璃专用树脂两项研究成果实现产业化,年创产值5000万元以上,2002年获教育部高等学校优秀骨干教师奖,2004年入选教育部新世纪优秀人才支持计划。
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355
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成果数
8
【期刊论文】Effects of copper on the activity and kinetics of free and immobilized acid phosphatase
史林启, Q. Huanga, b, H. Shindob, *
Soil Biology & Biochemistry 32(2000)1885-1892,-0001,():
-1年11月30日
Heavy metal pollution presents a major hazard to the soil environment. Studies have shown that the activities of a variety of soil enzymes are inhibited by heavy metals. However, little information is available concerning the effect of heavy metals on the activity of enzymes immobilized by different soil constituents. The main objective of this work was to investigate the effects of copper on the activity and kinetic properties of acid phosphatase both free and immobilized on two variable-charge soil clays and the minerals kaolin, goethite and manganese oxide. The effect of different forms of copper on enzyme activity was also examined. In the presence of copper chloride, the activity of free and immobilized enzymes was inhibited at copper concentrations of 0.005-0.8 mM at pH 5.0 and inhibition increased at pH 6.0. The inhibitory effect of copper chloride was greater on the enzymes bound by the two soil clays and kaolin than those by goethite and MnO2. Addition of copper chloride decreased both the Km values and the Vmax/Km ratios of free and all forms of immobilized enzymes, and showed mixed type inhibition kinetics. Comparing the effect of different forms of Cu, the residual activities of free enzyme and soil clay-enzyme and kaolin-enzyme complexes were higher when copper citrate was used than with copper chloride. The reverse was true for the enzymes immobilized on goethite and MnO2. These results indicate that the inhibition by Cu of enzymes immobilized on soil components are influenced by the properties of the adsorbent and the form of Cu, as well as pH.
Acid phosphatase, Copper, Enzyme activities, Enzyme kinetics, Soil clay, Kaolin, Oxides
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【期刊论文】Binding and Transformation of Extracellular DNA in Soil*1
史林启, CAI Peng, HUANG Qiao-Yun, *, ZHANG Xue-Wen and CHEN Hao
Pedosphere 14 (1): -, 2005,-0001,():
-1年11月30日
DNA is the genetic material of various organisms. Extracellular DNA adsorbed or bound on surface-active particles in soils has been shown to persist for long periods against nucleases degradation and still retain the ability to transform competent cells. This paper reviews some recent advances on the binding and transformation of extracellular DNA in soils, which is fundamental to understanding the nature of the soil, regulating biodiversity, and assessing the risk of releasing genetically engineered microorganisms (GEMs) as well as being belpful for development of the genetic evohtional theory of bacteria. Several inlluencing factors, such as soil pH, ionic strength, soil surface properties, and characteristics of the DNA polymer, are discussed. To date, the understanding of the type of molecular binding siLes and the conformation of adsorbed and bound DNA to soil particles is still in its infancy.
adsorption,, binding,, extracellular DNA,, soil,, transformation
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史林启, HUANG QIAOYUN, CIIEN WENLI, L. GANFREDA and A. VIOLANTE
Pcdosphcrc 12 (4): 339~348, 2002,-0001,():
-1年11月30日
The aim of this work was to study the influence of phosphate and citrate, which are common inorganic and organic anioilS in soils, on the adsorption of acid phosphatasc by kaolin, goethite and tile colloids separated fi'onl yellow-brown soil (YBS) and latosol (LS) in central-south China. The YBS colloid has the major clay mineral composition of 1.4 nm mineral, illite and kaolinite while the LS colloid mainly contains kaolinite and oxides. Thc adsorption isotherm of acid phosphatase on the exanlined soil colloids and minerals fitted to the Langmuir model. Tbe amounl of enzyme adsorbed in the absence of ligands was in the order of YBS colloid>LS colloid>kaolin≈gocthite. In the presence of phosphate or citrate, the amounts of the enzyme adsorbed followed the sequence YBS colloid>kaolin>LS colloid>goethite. The presence of ligands also decreased the binding energy between the enzyme and soil colloids or minerals. With the increase of ligand concentration from 10mmol L-1 to 400m mol L-1 different bchaviors for the adsorption of enzyme were found in the colloid and mineral systems studied. A sharp decrease in enzyme adsorption was observed on goethite while gradual decreases of enzynle adsorption were recorded in the two soil colloid systenls. However, no any decrease was found for the amount of enzyme adsorbed on kaolin at higher ligand concentrations. When phosphate or citrate was introduced to the system before the addition of enzyme, the ligarl (Is usually enhanced the adsorption of enzyme. The results obtained in tills study suggested the important role of kaolinite mineral in the adsorption of enzyme molecules in acidic soils in the presnce of various ligands.
acid phosphatase,, adsorption,, goethite,, kaolin,, soil colloid
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【期刊论文】Adsorption of Cadmium by Soil Colloids and Minerals in Presence of Rhizobia*1
史林启, HUANG QIAOYUN, WU JIANMEI, CHEN WENLI and LI XUEYUAN
Pedosphere 10 (4): 299~307, 2000,-0001,():
-1年11月30日
Experiments were conducted to study the adsorption of Cd on two soil colloids (red soil and yellowbrown soil) and three variable-charge minerals (goethite, noncrystalline Fe oxide and kaolin) in the absence and presence of rhizobia. The tested strain Rhizobium fredll C6, tolerant to 0.8 mmol L-1 Cd, was selected from 30 rhizobial strains. Results showed that the isotherms for the adsorption of Cd by examined soil colloids and minerals in the presence of rhizobia could be described by Langmuir equation. Within the range of the numbers of rhizobial ceils studied, the amount of Cd adsorbed by each system increased with increasing rhizobial cells. Greater increases for the adsorption of Cd were found in red soil and kaolin systems. Rhizobia influence on the adsorption of Cd by examined soil colloids and minerals was different from that on the adsorption of Cu. The presence of rhizobia increased the adsorption affinity of soil colloids and minerals for Cd, particularly for the goethite and kaolin systems. The discrepancies in the influence of rhizobia on the adsorbabliity and affinity of selected soil colloids and minerals for Cd suggested the different interactions of rhizobia with various soll components. It is assumed that bacterial biomass plays an important role in controlling the mobility and bioavallability of Cd in soils with kaolinite and goethite as the major colloidal components, such as in variablecharge soil.
cadmium adsorption,, iron oxide,, kaolin,, rhizobia,, soil colloid
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【期刊论文】Adsorption and redox reactions of heavy metals on Fe-Mn nodules from Chinese soils
史林启, Wenfeng Tan, Fan Liu ∗, Xionghan Feng, Qiaoyun Huang, Xueyuan Li
Journal of Colloid and Interface Science 284(2005)600-605,-0001,():
-1年11月30日
Adsorption of heavy metals and redox reactions of Cr(W) ions on Fe-Mn nodules from five soils of China were investigated by chemical analysis, equilibrium adsorption/redox, and X-ray photoelectron spectroscopy (XPS). Results show that Mn is mainly present as Mn3+ and Mn4+ forms in Fe-Mn nodules. The maximum adsorption amounts for different heavy metal ions follow the order Pb2+≈Cu2+>Zn2+>Co2+>Ni2+>Cd2+. The adsorption capacity for heavy metals by Fe-Mn nodules from calciaquert in Shandong province (N5-1) is the highest, while that from hapludalf in Shandong province (N6-1) is the lowest. About 44-100% of the heavy metals adsorbed on Fe-Mn nodules were dissolved in 0.1 mol/L hydroxylamine hydrochloride (HAHC). The maximum amounts of Cr(VI) production by Fe-Mn nodules follow the order of N1-1 (69 mmol/kg)> N4-1 (57 mmol/kg)> N2-1 (52 mmol/kg)> N5-1 (44 mmol/kg). Based on the content of MnO2 in Fe-Mn nodules dissolved in HAHC, the amount of Cr(VI) production by Mn oxides in N1-1, N2-1, N4-1, and N5-1 is 326, 624, 726, and 482 mmol/kg (MnO2), respectively. We propose that the amounts of Cr(VI) production through oxidation Cr(Ⅲ) by Mn oxides are related to the types of Mn oxides in Fe–Mn nodules.
Manganese oxide, Nodule, Adsorption, Redox, Heavy metal, Soil
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史林启, Qiaoyun Huang, * Wenli Chen, and Xuejun Guo
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS Vol. 35, Nos. 7 & 8, pp. 947-960, 2004,-0001,():
-1年11月30日
Red soil and cinnamon soil were collected from Chenzhou of Hunan and Gongyi of Henan, respectively. Soils were treated with Cu(NO3)2, Zn(NO3)2 or Cd(NO3)2, respectively, for two weeks. Rhizobium fredii strain HN01 was inoculated into the two soils polluted with three heavy metals. Sequential extraction method was employed to investigate the forms of copper (Cu), zinc (Zn), and cadmium (Ca) in the examined soils with the absence and presence of rhizobia. Results showed that the total amount of solid-bound Zn decreased 10% after the inoculation. The decrease for the amount of Zn associated with carbonate, manganese (Mn) oxides, and organic matter fraction was from 9 to 26%. No significant change was observed for the total amount of Zn combined with solid phase of red soil in the presence of rhizobia. However, the amount of specifically adsorbed and Mn oxides bound Zn decreased, while the amount of exchangeable Zn increased. Inoculation of rhizobia depressed the release of Cu to the soil solution and increased the total amount of Cu associated with solid phase in cinnamon soil. The increase for the amount of exchangeable Cu and the Cu in fractions of carbonate, Mn oxides, and organic matter ranged from 20 to 54%. There was no significant change for the level of Cd in the solution in both soils after rhizobia inoculation. The amount of Cd in the fractions of exchangeable and organic increased 22 and 11%, while that in the fractions of specific and Mn oxides decreased 14 and 29%, respectively. The different influence of rhizobia on the distribution of three heavy metals in two soils was mainly ascribed to the growth status and pH changes exerted by the metabolites of rhizobia. These data are helpful for the understanding of the chemical behavior and biogeochemical cycle of heavy metals affected by microorganisms in soil environment, which is fundamental for heavy metal bioremediation.
Soil, Heavy metal fractionation, Rhizobia, Copper, Zinc, Cadmium.,
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史林启, Qiaoyun Huang a, *, Zhenhua Zhao b, Wenli Chen b
Chemosphere 52(2003)571-579,-0001,():
-1年11月30日
Adsorption of acid phosphatase on goethite, kaolinite and two colloids from the soils in central and south China in the presence of organic acids and phosphate was studied. With the increase of anion concentration, the ability in decreasing enzyme adsorption followed the sequence: phosphate>tartrate>oxalate>acetate. Acetate showed promotive effect on enzyme adsorption at lower anion concentrations whereas oxalate, tartrate and phosphate compete effectively with enzyme in a broad range of anion concentration. The adsorption isotherms of enzyme in most of the anionic systems studied conformed to the Langmuir equation. Phosphate reduced the affinity of enzyme on goethite more significantly than the other anions. However, tartrate decreased the affinity of enzyme on soil colloids and kaolinite to a greater extent than phosphate, oxalate and acetate. This observation suggested that the impact of anions on enzyme adsorption varies with anionic type and the surface characteristics of soil components. The influence of the addition order of ligand on enzyme adsorption was found greater in tartrate and phosphate systems. In general, simultaneous introduction of ligand and enzyme into the system had the lowest enzyme adsorption, showing more competition between ligand and enzyme molecules in this system. Data from this work indicated that the status and activity of enzyme in certain soil microenvironments especially the rhizosphere where various organic and inorganic ligands are active can be altered and may be completely different from the bulk soil.
Acid phosphatase, Soil colloid, Mineral, Adsorption, Organic acid, Phosphate
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史林启, Wenli Chen
Biol Fertil Soils (2004) 40: 306-312,-0001,():
-1年11月30日
Soil samples were collected at an altitude of 500, 1,060, 1,500, 1,950, 2,400 and 3,100m, respectively, from Shennongjia, a forest reserve in Hubei province (central China). Their corresponding pHs were 5.50, 4.91, 5.64, 5.28, 5.49 and 4.60. By using a plant trap method, a total of 25 soybean rhizobia were isolated from the soil above an altitude of 1,500 m and all identified to be Sinorhizobium fredii. Their genetic biodiversity was characterized by 16S-23S rDNA internally transcribed spacer (ITS) region polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and random amplification DNA (RAPD) analysis. All the tested strains produced a 2.1 kb 16S-23S rDNA ITS fragment. After digestion with three restriction endonucleases (HaeⅢ, MspI and CfoI), respectively, great variations in 16S-23S rDNA ITS PCR-RFLP patterns were observed. The tested strains could be differentiated into 11 ITS genotypes. The genotypes of rhizobia were not related to geographical location. Twelve primers were applied to RAPD analysis and a dendrogram was obtained, showing that all the strains (including reference strain S. fredii USDA205) were divided into two diverging groups. Moreover, each group could be further divided into two subgroups. Both RAPD and 16S-23S rDNA ITS PCR-RFLP analysis indicated that a high degree of genetic diversity existed among S. fredii strains isolated from Shennongjia virgin soils. Since Shennongjia is an unexploited forest region in central China and the gene centre of soybean is located in China, the symbiotic genes harboured by these strains may be of great importance and the rich diversity of these strains might contribute to the adaptation of soybean to an alpine environment.
Biodiversity
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