杨光圣
主要从事油菜雄性不育和杂种优势利用研究
个性化签名
- 姓名:杨光圣
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
农艺学
- 研究兴趣:主要从事油菜雄性不育和杂种优势利用研究
杨光圣,教授,博士生导师,1962年4月出生。1998年获博士学位,1987年硕士研究生毕业以来一直在华中农业大学工作,先后多次赴德国、英国、加拿大等国进修或合作研究。
主要从事油菜雄性不育和杂种优势利用研究,将常规技术与生物技术相结合成功培育出一系列油菜新品种,在长江中下游地区广泛种植,并与瑞典、加拿大等国合作成功培育出适合这些国家种植的油菜新品种,研究成果,先后获国家科技进步一、二等奖及其他与系列奖励。入选"国家百千万人才工程"第一、二层次及“农业部神农计划”人选。目前承担科技部和农业部的多项项目,如国家863、973,农业部跨越计划等科研课题。
已指导(含合作指导)博士生2名,硕士生3名,在读博士生5名(含合作指导),硕士生8名。
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主页访问
2073
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0
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成果阅读
651
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成果数
17
杨光圣, B. YU, P. LIU, D. HONG, Q. HE and G. YANG,
Plant Breeding 129, 39-44(2010),-0001,():
-1年11月30日
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is one of the most severe diseases of rapeseed in China and other major growing regions. The objective of this study was to improve the S. sclerotiorum resistance of ‘Hui5200’, an elite ‘Polima’ CMS restorer line, by introgression and fixation of resistance alleles from the partially resistant cultivar ‘NingRS-1’ via phenotypic selection (PS), markerassisted background selection (MAB) and microspore culture. A progeny designated as ‘RSH’ with greatly improved Sclerotinia resistance and a similar genetic background as ‘Hui5200’ was obtained by two backcrosses and one selfing. From a selected elite progeny line (named as 7-5) double haploid (DH) lines were developed. By three cycles of PS considering economic traits and genetic distance analysis, four resistant DH restorer lines with elite economic traits were finally selected. The obtained resistant restorer lines have been used to produce commercial F1 hybrids. The results indicated that backcrossing plus PS and MAB is effective and suitable for improving resistance of rapeseed to S. sclerotiorum.
Brassica napus-Sclerotinia sclerotiorum-Sclerotinia stem rot-rapeseed-phenotypic selection-marker-assisted background selection-microspore culture-resistance breeding
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杨光圣, Y. Z. Xie, D. F. Hong, Z. H. Xu, P. W. Liu and G. S. Yang,
Plant Breeding 127, 145-149(2008),-0001,():
-1年11月30日
A recessive epistatic genic male sterility (REGMS) two-type line, 9012AB, has been used for rapeseed hybrid seed production in China. The male sterility of 9012AB is controlled by two recessive duplicate sterile genes (ms1 and ms2) interacting with one recessive epistatic suppressor gene (esp). Homozygosity at the esp locus (espesp) suppresses the expression of the recessive male sterility trait in homozygous ms1ms1ms2 ms2 plants. In this study, we used a combination of bulked segregant analyses and amplified fragment length polymorphism (AFLP) to identify markers linked to the suppressor gene in a BC1 population. From the survey of 1024 AFLP primer combinations, eight markers tightly linked to the target gene were identified. The two closest markers flanking both sides of Esp, P9M5370 and S16M14780, had a genetic distance of 1.4 cM and 2.1 cM, respectively. The AFLP fragment from P4M8190, which co-segregated with the target gene was converted into a sequence characterized amplified region marker. The availability of linked molecular markers will facilitate the utilization of REGMS in hybrid breeding in Brassica napus.
Brassica napus-molecular marker-temporary maintainer line-marker-assisted selection
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杨光圣, D. F. Hong, J. Liu, G. S. Yang, and Q. B. He
Plant Breeding 127, 69-73(2008),-0001,():
-1年11月30日
Rs1046AB is a dominant genic male sterility (DGMS) line in rapeseed, in which the sterility has always been thought to be conditioned by the interaction of a male sterility gene (Ms) and its non-allelic restorer gene (Rf). This system provides not only a tool for assisting in recurrent selection but also a promising system for hybrid production. Based on previous studies, two amplified fragment length polymorphism markers linked with the Ms gene were converted into a dominant and a co-dominant sequence characterized amplified region (SCAR) marker, respectively. The putative linear order relationship of three dominant SCAR markers with the same genetic distance from the Rf gene, was also determined by an examination of whether the homologues of these markers are present or not in different lines carrying Rf. A bigger fragment generated by the closest marker linked to the Rf gene was observed in all lines carrying the recessive allele rf, suggesting that this marker is a co-dominant marker, which was further confirmed by nucleotide sequence comparison of these fragments. SCAR markers specific for Ms and Rf will be especially valuable in marker-assisted DGMS three-line breeding.
Brassica napus-sequence characterized amplified region-marker-assisted selection-genic male sterility
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杨光圣, Z. X. Fan, W. X. Lei, D. F. Hong, J. P. He, L. L. Wan, Z. H. Xu, P. W. Liu and G. S. Yang,
Plant Breeding 126, 297-301(2007),-0001,():
-1年11月30日
Over the past decade, the polima cytoplasmic male sterility (pol CMS) three-line and two-line systems have been developed for the production of hybrid seed in Brassica napus oilseed rape in China. The discovery of the novel pol CMS restorer line FL-204 is described here. It restores male fertility of hybrid plants in the pol CMS system, but hybrid seed production can only be carried out under autumn sowing in Wuhan in south China under moderate temperatures at flowering. The restorer cannot be used as a male for hybrid seed production in northwestern China (Gansu) under spring sowing conditions, because there it is more or less male sterile due to high temperatures at flowering. Because of this behaviour, it is referred to as a fertility temperature-sensitive restorer (FTSR) in this paper. F2, BC1 as well as double haploid populations were constructed to determine the inheritance of fertility restoration of FL-204 in the autumn at Wuhan and under spring sowing conditions at Gansu, respectively. Deviations from Mendelian genetics were observed. It was hypothesized that the change of fertility was the result of the interaction between nuclear genes [restoring gene (Rf) and temperature-sensitive genes (ts)] and the cytoplasm. The Rf gene in FL-204 was incapable of restoring male fertility of pol CMS lines under spring sowing conditions at Gansu where it is inactivated by the recessive ts gene present in FL-204. However, the ts gene(s) could be non-functional under moderate temperature conditions at flowering at Wuhan which allows full expression of male fertility in FL-204. The recessive ts gene(s) can only be expressed in plants containing the pol sterile cytoplasm. A method for the utilization of the FTSR pol CMS restorer FL-204 for the production of hybrid seed in B. napus oilseed rape is proposed.
Brassica napus-polima cytoplasmic male sterility-restorer-temperature-sensitive gene-fertility
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杨光圣, L. Y. Yang, P. W. Liu and G. S. Yang,
Plant Breeding 125, 368-371(2006),-0001,():
-1年11月30日
A novel Pol CMS named Polima temperature-sensitive cytoplasmic male sterile (Pol TCMS) is sterile at high temperatures and partially fertile at low temperatures. Its temperature sensitivity is controlled by several minor genes, and probably influenced by the genetic background, making it quite difficult to breed a stable Pol TCMS line through the conventional breeding methods. The present study aimed to obtain stable homozygous Pol TCMS lines by microspore culture. The highest frequency of embryogenesis was induced from cultures of buds sampled at 4-7 days before first blossom. The second method of microspore cultures mentioned in this study could significantly increase the production of embryos. Analysis of fatty acid composition, glucosinolate content and genetic diversity by simple sequence repeat (SSR) markers indicated that microspore culture was effective for selecting Pol TCMS lines.
Brassica napus-breeding-microspore culture-Polima cytoplasmic male sterility
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杨光圣, L. P. Ke, Y. Q. Sun, D. F. Hong, P. W. Liu and G. S. Yang,
Plant Breeding 124, 367-370(2005),-0001,():
-1年11月30日
The commercial utilization of heterosis in seed yield by means of hybrid varieties is of great importance for increasing oilseed rape production in China. This requires a functional system for the production of hybrid seed. The Brassica napus oilseed rape line 9012AB is a recessive epistatic genic male sterility (GMS) two-type line, in which the sterility is controlled by two pairs of recessive duplicate sterile genes (ms1 and ms2) interacting with one pair of a recessive epistatic inhibitor gene (rf). Homozygosity at the rf locus (rfrf) inhibits the expression of the recessive male sterility trait in homozygous ms1ms1ms2ms2 plants. This study was conducted to identify molecular markers for one of the male fertility/sterility loci in the B. napus male sterility line 9012AB. Sterile bulk (BS) and fertile bulk (BF) DNA samples prepared from male sterile and male fertile plants of the homozygous two-type line 9012AB were subjected to amplified fragment length polymorphic (AFLP) analysis. A total of 256 primer combinations were used and seven markers tightly linked to one recessive genic male sterile gene (ms) were identified. Among them, six fragments co-segregated with the target gene in the tested population, and the other one had a genetic distance of 4.3 cM. The markers identified in this study will greatly enhance the utilization of recessive GMS for the production of hybrid seed in B. napus oilseed rape in China.
Brassica napus-amplified fragment length polymorphic markers-gene male sterility-inhibitor-oilseed rape-recessive epistatic inhibitor-three-line hybridizing system
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【期刊论文】Molecular mapping of a dominant genic male sterility gene Ms in rapeseed Brassica napus)
杨光圣, G. Y. Lu, G. S. Yang, and T. D. Fu
Plant Breeding 123, 262-265(2004),-0001,():
-1年11月30日
Rs1046AB is a genic male sterile two-type line in rapeseed that has great potential for hybrid seed production. The sterility of this line is conditioned by the interaction of two genes, i.e. the dominant genic male sterility gene (Ms) and the suppressor gene (Rf). The present study was undertaken to identify DNA markers for the Ms locus in a BC1 population developed from a cross between a male-sterile plant in Rs1046AB and the fertile canola-type cultivar ‘Samourai’. Bulked segregant analysis was performed using the amplified fragment length polymorphism (AFLP) methodology. From the survey of 480 AFLP primer combinations, five AFLP markers (P10M13350, P13M8400, P6M6410, E7M1230 and E3M15100) tightly linked to the target gene were identified. Two of them, E3M15100 and P6M6410, located the closest, at either side of Ms at a distance of 3.7 and 5.9 cM, respectively. The Ms locus was subsequently mapped on linkage group LG10 in the map developed in this laboratory, adding two additional markers weakly linked to it. This suite of markers will be valuable in designing a markerassisted genic male sterility three-line breeding programme.
Brassica napus-AFLP markers-bulked segregant analysis-dominant genic male sterility gene-genetic mapping-marker-assisted selection (, MAS),
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31浏览
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【期刊论文】Evaluation Genetic Diversity of Hybrid Parents Developed in Brassic napus
杨光圣
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-1年11月30日
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