崔大祥
纳米材料的生物学效应与安全性,纳米诊断治疗技术,纳米药物,蛋白质结构与功能。
个性化签名
- 姓名:崔大祥
- 目前身份:
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学术头衔:
教育部“新世纪优秀人才支持计划”入选者, 博士生导师
- 职称:-
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学科领域:
生物化学
- 研究兴趣:纳米材料的生物学效应与安全性,纳米诊断治疗技术,纳米药物,蛋白质结构与功能。
崔大祥,男,安徽桐城人,1990年在第二军医大学获医学学士学位;1995年在第四军医大学获医学硕士学位;1998年获生化与分子生物学专业博士学位;毕业后在全军基因诊断技术应用研究所工作,2000年晋升为副教授。2001年底至2004年9月,德国Max Planck 研究所博士后与访问学者,获取德国Project Manager 资格证书与生物安全管理资格证书,是生物-纳米实验组组长。2004年7月,晋聘为上海交通大学教授,组建生物纳米工程研究室并任研究室主任。2007年9月起,成为Waseda大学客员教授。在Nano Letter, Cancer Research, Analytic Chemistry, Journal of Physical Chemistry C, Nanotechnology, Toxicology Letters, 中华医学杂志等国内外专业杂志上发表学术论文140余篇,邀请在国际会议上报告3次,获军队科技进步2等奖1项,省科技进步2等奖2项,国家教学成果2等奖1项,获欧洲与美国专利1项,参与编写出版专著10部,是2006年上海市浦江人才计划获得者。承担完成过国家自然科学基金,陕西省重点基金等项目,目前承担国家863重点课题1项,973子课题1项, 上海市纳米专项1项,是第四届世界细胞与分子生物学大会的“Nano-Biotechnology and Bio-safety”分会的主席,是Nanoscience杂志的编委。主要研究方向:纳米材料的生物学效应与安全性,纳米诊断治疗技术,纳米药物,蛋白质结构与功能。
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390
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成果数
8
【期刊论文】Effect of single wall carbon nanotubes on human HEK293 cells
崔大祥, Daxiang Cui a, c, ∗, Furong Tian a, Cengiz S. Ozkan b, Mao Wang a, Huajian Gao a
Toxicology Letters 155(2005)73-85,-0001,():
-1年11月30日
The influence of single-walled carbon nanotubes (SWCNTs) on human HEK293 cells is investigated with the aim of exploring SWCNTs biocompatibility. Results showed that SWCNTs can inhibit HEK293 cell proliferation, decrease cell adhesive ability in a dose-and time-dependent manner. HEK293 cells exhibit active responses to SWCNTs such as secretion of some 20-30 kd proteins to wrap SWCNTs, aggregation of cells attached by SWCNTs and formation of nodular structures. Cell cycle analysis showed that 25g/ml SWCNTs in medium induced G1 arrest and cell apoptosis in HEK293 cells. Biochip analysis showed that SWCNTs can induce up-regulation expression of cell cycle-associated genes such as p16, bax, p57, hrk, cdc42 and cdc37, down-regulation expression of cell cycle genes such as cdk2, cdk4, cdk6 and cyclin D3, and down-regulation expression of signal transduction-associated genes such as mad2, jak1, ttk, pcdha9 and erk. Western blot analysis showed that SWCNTs can induce down-regulation expression of adhesion-associated proteins such as laminin, fibronectin, cadherin, FAK and collagen IV. These results suggest that down-regulation of G1-assoicated cdks and cyclins and upregulation of apoptosis-associated genes may contribute to SWCNTs induced G1 phase arrest and cell apoptosis. In conclusion, SWCNTs can inhibit HEK293 cells growth by inducing cell apoptosis and decreasing cellular adhesion ability.
HEK293 cell, Single-walled carbon nanotube, Apoptosis, Biochip, Flow cytometry analysis, Western blot, Immunofluorescent analysis
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【期刊论文】Spontaneous Insertion of DNA Oligonucleotides into Carbon Nanotubes
崔大祥, Huajian Gao, * Yong Kong, and Daxiang Cui
Published on Web 03/19/2003,-0001,():
-1年11月30日
We report molecular dynamics simulations showing that a DNA molecule could be spontaneously inserted into carbon nanotube (CNT) in a water solute environment. The van der Waals and hydrophobic forces were found to be important for the insertion process, with the former playing a more dominant role in the DNA-CNT interaction. Our study suggests that the encapsulated CNT-DNA molecular complex can be further exploited for applications such as DNA modulated molecular electronics, molecular sensors, electronic DNA sequencing, and nanotechnology of gene delivery systems.
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【期刊论文】Encapsulation of Pt-labelled DNA Molecules inside Carbon Nanotubes
崔大祥, Daxiang Cui, Cengiz S. Ozkan, Sathyajith Ravindran, Yong Kong, Huajian Gao
MCB, vol.1, no.2, pp.113-121, 2004,-0001,():
-1年11月30日
Experiments on encapsulating Pt-labelled DNA molecules inside multiwalled carbon nanotubes (MWCNT) were performed under temperature and pressure conditions of 400K and 3 Bar. The DNA-CNT hybrids were purified via agarose gel electrophoresis and analyzed via high resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray spectroscopy (EDX). The results showed that the Pt-labelled DNA molecules attached to the outside walls of CNTs could be removed by electrophoresis. The HR-TEM and EDX results demonstrated that 2-3% of the Pt-labelled DNA molecules were successfully encapsulated inside the MWCNTs. The experimental study complements our previous molecular dynamics simulations on encapsulation of single stranded DNA oligonucleotides inside single wall carbon nanotubes under similar conditions in water. The van der Waals interaction between CNT and Pt-labelled DNA is believed to be the main driving force for this phenomenon. The DNA-CNT molecular complex could be further explored for potential applications in bio-nanotechnology.
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【期刊论文】DNA-Templated Ordered Array of Gold Nanorods in One and Two Dimensions
崔大祥, Bifeng Pan, † Daxiang Cui, *, † Cengiz Ozkan, ‡ Ping Xu, † Tuo Huang, † Qing Li, † Hao Chen, † Fengtao Liu, † Feng Gao, † and Rong He†
J. Phys. Chem. C 2007, 111, 12572-12576,-0001,():
-1年11月30日
Many sensing/catalytic applications in nanotechnology require a programmed assembly of nanospheres/nanorods onto surfaces. The gold nanorods, formed by a seed-mediated, surfactant-assisted synthesis protocol, are stabilized in solution due to surface modification by the surfactant cetyltrimethylammonium bromide. DNAtemplated self-assembly of gold nanorods with different aspect ratios at different DNA concentration values has been studied using transmission electron microscopy. Under appropriate conditions such as aspect ratio and DNA concentration, gold nanorods assemble into one-and two-dimensional structures. A stable phase of side-by-side supramolecular assemblies was formed, and some of the double-layer assemblies extend to superlattices of nanorods. The resulting guide for designing statistically patterned arrays of nanoparticles suggests the possibility of fabricating spontaneously organized nanoscale devices.
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【期刊论文】Chemiluminescence of luminol catalyzed by silver nanoparticles
崔大祥, Hao Chen, Feng Gao∗, Rong He, Daxiang Cui∗
Journal of Colloid and Interface Science 315(2007)158-163,-0001,():
-1年11月30日
Silver nanoparticles (AgNPs) are synthesized by chemical reduction method and characterized by UV-vis spectra, transmission electron microscopy, and high performance particle sizer.We have found that AgNPs could enhance the chemiluminescence (CL) intensity of luminol-H2O2 system. In this reaction, luminol intermediate is generated under alkaline condition on the surface of AgNPs in luminol-H2O2 system and enhances CL intensity. To validate the reaction mechanism, AgNPs are bound with thioglycolic acid (Ag-HSCH2COOH) and then joined to BSA protein (Ag-BSA). We investigate the CL intensity in the presence of Ag-HSCH2COOH or Ag-BSA comparing with that in the presence of AgNPs and conclude the catalytic reaction take place on the surface of AgNPs.
Silver nanoparticles, Chemiluminescence, Luminol, Mechanism
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【期刊论文】Fluoroimmunoassay for Antigen Based on Fluorescence Quenching Signal of Gold Nanoparticles
崔大祥, Limei Ao, Feng Gao, * Bifeng Pan, Rong He, and Daxiang Cui*
Analytical Chemistry, Vol. 78, No. 4, February 15, 2006,-0001,():
-1年11月30日
A unique, sensitive, and highly specific fluoroimmunoassay system for antigen detection using gold and magnetic nanoparticles has been developed. The assay is based on the fluorescence quenching of fluorescein isothiocyanate caused by gold nanoparticles coated with monoclonal antibody. To demonstrate its analytical capabilities, the magnetic nanoparticles were coated with anti-r-fetoprotein polyclonal antibodies, which specifically bound with r-fetoprotein. Gold nanoparticles coated with anti-r-fetoprotein monoclonal antibodies could sandwich the r-fetoprotein captured by the magnetic nanoparticle probes. The sandwich-type immunocomplex was formed on the surface of magnetic nanoparticles and could be separated by a magnetic field. The supernatant liquid, which contained the unbound gold nanoparticle probes, was used to quench the fluorescence, and the fluorescence intensity of fluorescein isothiocyanate at 516 nm was proportional to the r-fetoprotein concentration. The result showed that the limit of detection of r-fetoprotein was 0.17 nM. This new system can be extended to detect target molecules with matched antibodies and has broad potential applications in immunoassay and disease diagnosis.
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【期刊论文】Characterization of BRCAA1 and Its Novel Antigen Epitope Identification
崔大祥, Daxiang Cui, , Guiqiu Jin, Tianwen Gao, Tianbai Sun, Furong Tian, Giovani Gomez Estrada, Huajian Gao, and Akinori Sarai
Cancer Epidemiol Biomarkers Prev 2004, 13 (7),-0001,():
-1年11月30日
Looking for novel breast cancer antigen epitopes is helpful for its treatment, diagnosis, and prevention. brcaa1 gene is mapped at 1q42.1-q43, its whole genome is 93.857 kb, including 18 exons and 17 introns. BRCAA1 protein is composed of 1,214 amino acids with 10 glycosylate sites, and shares 37% amino acid identity and an identical antigen epitope with Rb binding protein 1. The novel antigen epitope, SSKKQKRSHK, was predicted to locate in the region 610 to 619 sites, was synthesized, and its antibody was fabricated. Competent inhibition analysis showed that SSKKQKRSHK is the shortest effective peptide. The antigen epitope was mapped in the cytoplasm of MCF-7 cells. Immunohistochemistry analysis showed that the antigen epitope exhibited positive expression in 65% (39 of 60) breast cancer specimens and negative expression in 60 noncancerous tissues. Statistical analysis shows that its expression is closely associated with status of ER and PR, with sensitivity of 100% and specificity of 81%, and confidence interval of 85.9% to 96.9%. ELISA analysis showed that the mean absorbance of sera antibody titers from breast cancer patients and healthy donors were 0401 F 0.163 SD and 0.137 F 0.121 SD, respectively. Sixty-four percent breast cancer patient sera and 13% healthy donor sera had higher titer than mean titer of healthy donors, and there exists significant difference between breast cancer patients and healthy donors (P<0.001). In this study, a novel breast cancer antigen epitope, SSKKQKRSHK, is identified. Its expression is associated with characteristics that are themselves associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis.
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【期刊论文】A microarray-based gastric carcinoma prewarning system
崔大祥, Da-Xiang Cui, Li Zhang, Xiao-Jun Yan, Ling-Xia Zhang, Jun-Rong Xu, Yan-Hai Guo, Gui-Qiu Jin, Giovani Gomez, Ding Li, Jin-Rong Zhao, Fen-Chan Han, Ju Zhang, Jia-Le Hu, Dai-Ming Fan, Hua-Jian Gao
World J Gastroenterol 2005, 11 (9): 1273-1282,-0001,():
-1年11月30日
To develop a microarray-based prewarning system consisting of gastric cancer chip, prewarning data and analysis software for early detection of gastric cancer and pre-cancerous lesions.
Microarray, Prewarning, Gastric cancer
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