冯耀宇
从事多种寄生虫病的分子流行病学研究。
个性化签名
- 姓名:冯耀宇
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
微生物学
- 研究兴趣:从事多种寄生虫病的分子流行病学研究。
冯耀宇,女,1969年出生,现任同济大学生命科学与技术学院教授,博士生导师;以及同济大学海洋与地球科学学院兼职博士生导师。
于1990年和1993年分别获得南开大学微生物专业的本科和硕士学位,随后进入天津大学从事生物化工领域的教学与科研工作,曾参加天津市导向性重大科技项目和多项国家自然科学基金等项目的研究,并于1999年获得生物化工专业的博士学位。2000年初至2004年6月在新加坡国立大学土木工程系水研究中心从事博士后研究员工作,参加多项新加坡政府和新加坡国立大学研究项目, 主要从事环境微生物,尤其是水中新发寄生虫的检测工作,并取得国际领先的研究成果。研究对象广泛涉及微生物多个领域,主要包括细菌,病毒和原生动物等。研究目的包括新发现的水生病原菌的检测方法建立及应用多种水处理工艺去除这些病原菌。最新研究方向还包括雌性激素类化合物的生物检测方法建立及它们在水处理工艺中的去除研究。2004年6月至今作为教授、博导在同济大学工作期间,继续从事寄生虫病检测方面的研究。2005年9月至今在美国疾病预防控制中心作为国际新发传染病研究员,主要从事多种寄生虫病的分子流行病学研究。
近年来共主持国内项目2项, 国外项目3项,包括国际合作项目等,获科研基金总额近两百万元。在水体中细菌,病毒和原生动物检测和去除方面进行了长期研究,尤其在新发现的水体中寄生虫类病原菌的检测方法建立方面取得了骄人的成绩。研究成果修订了美国国家环保局的相应检测方法标准;并首次报道了运用新型纳米荧光材料对检测寄生虫的感染性进行免疫荧光标定的技术,从而建立了一种国际领先的可以同时测定多种对人类致病的隐孢子虫感染性的新方法;同时还报道了一种可以同时检测多种对人类致病的寄生虫的感染性、定量及种类区分的国际领先新型技术。此外,还首次构建了一种新型的可利用淀粉高产碱性蛋白酶的工程菌,并对酶的性质、纯化和生产工艺等进行了详尽研究,同时筛选出了甘露聚糖酶的高产菌株及对其进行了详尽的不同规模的生产和纯化工艺及产酶动力学研究,该工作使得这两种酶成功进行了技术转让。
主要成绩: 1.研究奖项:荣获2001年天津市自然科学一等奖。2. 期刊论文:国外重要学术刊物发表论文32篇(25余篇被SCI收录),其中某些期刊的SCI影响因子达6以上。3. 会议报告和论文:在国际学术会议上做特邀报告2次,发表国际会议论文10余篇,国内会议论文5篇。4. 期刊审稿:担任“Water Research”,“Biotechnology and Applied Biochemistry”和“Process Biochemistry”等国外重要学术刊物的审稿人。5.国际会议审稿:曾担任5个国际会议的特约审稿人。
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冯耀宇, Jiangyong Hu*, Yaoyu Feng, Say Leong Ong, Wun Jern Ng, Lianfa Song, Xiaolan Tan, Xiaona Chu
Journal of Microbiological Methods 58(2004)321-325,-0001,():
-1年11月30日
The U.S. Environmental Protection Agency has developed method 1623 for simultaneous detection of Cryptosporidium oocysts and Giardia cysts in water. Method 1623 includes four major steps: filtration, immunomagnetic separation (IMS), fluorescent antibody (FA) staining and microscopic examination. It was noted that the recovery levels following IMS-FA and FA staining were high, averaging more than 92.0% and 89.0% for C. parvum oocysts and G. lamblia cysts, respectively. In contrast, when the filtration step was incorporated, the recovery level of C. parvum oocysts declined significantly to 18.1% in seeded tap water, while a relatively high recovery level of 77.2% for G. lamblia cysts could still be achieved. Further study indicated that the recovery level of C. parvum oocysts could be enhanced significantly when an appropriate amount of silica particles was added to a water sample. The recovery level of C. parvum oocysts was affected by particle size and concentration. The optimal silica particle size was determined to be within the range of 5-40 Am, and the corresponding optimal silica concentration was 1.42g for 10-l tap water. When both G. lamblia cysts and C. parvum oocysts were spiked into the tap water sample containing the optimum amount of silica particles, the average recovery levels of oocysts and cysts were 82.7% and 75.4%, respectively. The results obtained clearly suggested that addition of an appropriate amount of silica particles could improve the recovery level of C. parvum oocysts significantly and yet there was no noticeable deleterious effect on the recovery level of G. lamblia cysts. Further study indicated that the rotation time in the IMS procedure using the Dynal GC-Combo IMS kit (which was recommended in method 1623) was important for G. lamblia cyst detection. In contrast, the recovery level of C. parvum oocysts was not affected by the rotation time. Furthermore, it was found that the recovery levels of C. parvum oocysts using methods 1622 and 1623 were quite close although different IMS kits were used in the two methods.
Cryptosporidium, Filtration, Giardia, Immunomagnetic separation, Recovery
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冯耀宇, Yao Yu Feng, Say Leong Ong, *, Jiang Yong Hu, Lian Fa Song, Xiao Lan Tan, and Wun Jern Ng
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Apr. 2003, p. 1898-1903,-0001,():
-1年11月30日
Cryptosporidium parvum can be found in both source and drinking water and has been reported to cause serious waterborne outbreaks which threaten public health safety. The U.S. Environmental Protection Agency has developed method 1622 for detection of Cryptosporidium oocysts present in water. Method 1622 involves four key processing steps: filtration, immunomagnetic separation (IMS), fluorescent-antibody (FA) staining, and microscopic evaluation. The individual performance of each of these four steps was evaluated in this study. We found that the levels of recovery of C. parvum oocysts at the IMS-FA and FA staining stages were high, averaging more than 95%. In contrast, the level of recovery declined significantly, to 14.4%, when the filtration step was incorporated with tap water as a spiking medium. This observation suggested that a significant fraction of C. parvum oocysts was lost during the filtration step. When C. parvum oocysts were spiked into reclaimed water, tap water, microfiltration filtrate, and reservoir water, the highest mean level of recovery of (85.0%±5.2% [mean±standard deviation]) was obtained for the relatively turbid reservoir water. Further studies indicated that it was the suspended particles present in the reservoir water that contributed to the enhanced C. parvum oocyst recovery. The levels of C. parvum oocyst recovery from spiked reservoir water with different turbidities indicated that particle size and concentration could affect oocyst recovery. Similar observations were also made when silica particles of different sizes and masses were added to seeded tap water. The optimal particle size was determined to be in the range from 5 to 40μm, and the corresponding optimal concentration of suspended particles was 1.42g for 10 liters of tap water.
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冯耀宇, Yaoyu Fenga, ∗, Zhimin Heb, Say Leong Onga, Jiangyong Hua, Zhigang Zhangb, Wun Jern Nga
Enzyme and Microbial Technology 32(2003)282-289,-0001,():
-1年11月30日
The effects of cultivation temperature, aeration rate, and agitation speed on the production of β-mannanase by Bacillus licheniformis NK-27 in a batch fermenter were investigated in this study. Results revealed that temperature was the most significant factor in terms of its effect on β-mannanase production. It influenced β-mannanase production by affecting the other parameters including bacteria growth, pH, dissolved oxygen, total and reducing sugars. Agitation speed and aeration rate could affect dissolved oxygen concentration which in turn affected cell growth and β-mannanase production. A maximum βmannanase activity of 212.0Uml−1 was attained in 36h of cultivation when aeration rate, agitation speed, and temperature were controlled at 0.75vvm, 600rpm, and 30℃, respectively. The maximum β-mannanase activity in the fermenter was close to that obtained from the shake flask fermentation study (198.2Uml-1). However, the duration of fermentation cycle in the fermenter study was shorter than the corresponding duration obtained from the shake flask experiment by 12h.
βMannanase, Bacillus licheniformis, Aeration rate, Agitation speed, Temperature
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【期刊论文】Synthesis and characterization of [Ga(TPP)H] (TPP
冯耀宇, Yaoyu Feng*, Say-Leong Ong, Jiangyong Hu, Wun-Jern Ng
Inorganic Chemistry Communications 6(2003)466-468,-0001,():
-1年11月30日
The synthesis, spectroscopic and structural characterization of the stable gallium hydride compound [Ga(TPP)H] (TPP
Gallium, Porphyrin, Hydride, Crystal structure
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