汪世龙
1、多肽及活性化合物作为分子药物的研制。2、纳米载药体系及其生物效应的研究。3、高产酒精菌种的优化和机理研究。
个性化签名
- 姓名:汪世龙
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
生物化学
- 研究兴趣:1、多肽及活性化合物作为分子药物的研制。2、纳米载药体系及其生物效应的研究。3、高产酒精菌种的优化和机理研究。
汪世龙,男,1964年7月出生,教授,博导,同济大学生命科学与技术学院副院长,中国第九届生物化学与分子生物学理事、首届蛋白质专委会委员、上海市第十届生物化学与分子学学会理事,民建同济大学委员会委员,第三支部书记。1988年7月毕业于阜阳师范学院(获学士学位),1996年6月博士毕业于中国科技大学,1996年6月来同济大学工作至今。其间1999年9月至2000年5月在杨浦区科委挂职锻炼,任杨浦区科委副主任,2000年5月至2002年1月在美国Cornell University生物化学系从事博士后研究工作。现已主持和参与国家自然科学基金,上海市科技攻关项目10余项,已发表学术论文80余篇,其中被SCI,EI摘录的40余篇 ,申请专利8项,已指导出站的博士后1名, 毕业的博士生1名,硕士生6名。
主要研究成果和研究方向:
1、多肽及活性化合物作为分子药物的研制: 根据HIV-1膜融合的机理,分别从四个方面设计了系列多肽来作为HIV-1膜融合的抑制剂。即利用前体加工酶的抑制剂来抑制gp160的裂解;利用类CD4的多肽竞争gp120与CD4的结合;类gp41的碳端多肽来竞争抑制gp41分子的构象变化;上述三类多肽的活性中心组合成的组合多肽,现已取得了阶段性的成果。另外对天然植物成分鬼臼毒素的治疗肿瘤机理进行了研究。
2、纳米载药体系及其生物效应的研究。针对层状双氢氧化物和固体脂质纳米粒等一些纳米粒子的特殊性能,进行了多种类型纳米材料的制备、控释和靶向性质的探讨,对所得纳米粒子在治疗肿瘤药物和多肽药物载运的效果上进行了尝试,获得了一些具有潜在应用前景的纳米新材料,同时对常用纳米粒子的生物效应进行了详细研究。
3、高产酒精菌种的优化和机理研究:利用一些极端条件对现有菌种和酶等进行诱变,期望获得一些优异的菌株和高效的酶,从而提高酒精生产的产量,并从理论上进行了探讨。现已取得了阶段性的成果。
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主页访问
1599
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关注数
0
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成果阅读
406
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成果数
6
汪世龙, Shilong Wang, § Joanne York, ‖ Wei Shu, § Marisa O. Stoller, ‖ Jack H. Nunberg, *, | and Min Lu*, §
Biochemistry 2002, 41, 7283-7292,-0001,():
-1年11月30日
The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein complex (gp120-gp41) promotes viral entry by mediating the fusion of viral and cellular membranes. Formation of a stable trimer-of-hairpins structure in the gp41 ectodomain brings the two membranes into proximity, leading to membrane fusion. The core of this hairpin structure is a six-helix bundle in which three carboxylterminal outer helices pack against an inner trimeric coiled coil. Here we investigate the role of these conserved interhelical interactions on the structure and function of both the envelope glycoprotein and the gp41 core. We have replaced each of the eight amino acids at the buried face of the carboxyl-terminal helix with a representative amino acid, alanine. Structural and physicochemical characterization of the alanine mutants shows that hydrophobic interactions are a dominant factor in the stabilization of the six-helix bundle. Alanine substitutions at the Trp628, Trp631, Ile635, and Ile642 residues also affected envelope processing and/or gp120-gp41 association and abrogated the ability of the envelope glycoprotein to mediate cell-cell fusion. These results suggest that the amino-terminal region of the gp41 outer-layer R-helix plays a key role in the sequence of events associated with HIV-1 entry and have implications for the development of antibodies and small-molecule inhibitors of this conserved element.
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汪世龙, Jie Liu, Shilong Wang, James A. Hoxie, Celia C. LaBranche and Min Lu*
,-0001,():
-1年11月30日
The human and simian immunodeficiency viruses (HIV and SIV) envelope glycoprotein consists of a trimer of two noncovalently and weakly associated subunits, gp120 and gp41. Upon binding of gp120 to cellular receptors, this labile native envelope complex undergoes conformational changes, resulting in a stable trimer-of-hairpins structure in gp41. Formation of the hairpin structure is thought to mediate membrane fusion by placing the viral and cellular membranes in close proximity. An in-vitro-derived variant of SIVmac251, denoted CPmac, has acquired an unusually stable virion-associated gp120-gp41 complex. This unique phenotype is conferred by five amino-acid substitutions in the gp41 ectodomain. Here we characterize the structural and physicochemical properties of the N40(L6)C38 model of the CPmac gp41 core. The 1.7
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【期刊论文】鬼臼毒素及VP-16化学活性的激光光解与脉冲辐解研究*
汪世龙, 孙晓宇, 张超杰**, 王玫, 倪亚明, 姚思德
中国科学(B辑),2002,32(2):148~155,-0001,():
-1年11月30日
运用激光光解与脉冲辐解技术,对用于肿瘤治疗的鬼臼毒素(PPT)和依托泊试(VP-16)的化学活性进行了详细的研究结果表明鬼臼毒素及(VP-16)经248nm的激光激发可光电离,且光电离的量子产额比较高,有很强的光敏性,而且还有多个活性基团分别与水合电子、氢原子、经基自由基作用生成不同的瞬态粒子,并推断出相应的反应机理为医学工作者进一步了解、利用鬼臼毒素和VP-16,探讨其抗肿瘤机理提供科学的参考,也为化学工作者进一步合成高效、低毒的抗肿瘤药物提供新思路。
鬼臼毒素 VP-16 激光光解 脉冲辐解 抗癌活性
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汪世龙, MIN LU, *, MARISA O. STOLLER, , SHILONG WANG, JIE LIU, MELINDA B. FAGAN, † AND JACK H. NUNBERG*
JOURNAL OF VIROLOGY, Nov. 2001, p. 11146-11156,-0001,():
-1年11月30日
Membrane fusion by human immunodeficiency virus type 1 (HIV-1) is promoted by the refolding of the viral envelope glycoprotein into a fusion-active conformation. The structure of the gp41 ectodomain core in its fusion-active state is a trimer of hairpins in which three antiparallel carboxyl-terminal helices pack into hydrophobic grooves on the surface of an amino-terminal trimeric coiled coil. In an effort to identify amino acid residues in these grooves that are critical for gp41 activation, we have used alanine-scanning mutagenesis to investigate the importance of individual side chains in determining the biophysical properties of the gp41 core and the membrane fusion activity of the gp120-gp41 complex. Alanine substitutions at Leu-556, Leu-565, Val-570, Gly-572, and Arg-579 positions severely impaired membrane fusion activity in envelope glycoproteins that were for the most part normally expressed. Whereas alanine mutations at Leu-565 and Val-570 destabilized the trimer-of-hairpins structure, mutations at Gly-572 and Arg-579 led to the formation of a stable gp41 core. Our results suggest that the Leu-565 and Val-570 residues are important determinants of conserved packing interactions between the amino- and carboxyl-terminal helices of gp41. We propose that the high degree of sequence conservation at Gly-572 and Arg-579 may result from selective pressures imposed by prefusogenic conformations of the HIV-1 envelope glycoprotein. Further analysis of the gp41 activation process may elucidate targets for antiviral intervention.
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汪世龙, 王玫, 孙晓宇, 张震, 李敏, 倪亚明, 姚思德, 王文峰
高等学校化学学报,2003,24(11):2014~2018,-0001,():
-1年11月30日
分析了鬼臼毒素及其衍生物与还原性自由基(氢自由基、水合电子)作用、氧化性自由基羟基自由基和激光等作用后产生的各种瞬态粒子,对各粒子的生成和衰减过程进行跟踪,获得了各瞬态粒子的生成和衰减速率常数,进一步探讨了鬼臼毒素治疗肿瘤的构效关系。
鬼臼毒素及其衍生物, 脉冲辐解, 激光光解, 瞬态粒子
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【期刊论文】原位插层聚合法制备聚丙烯酰胺/α-磷酸锆纳米复合材料及其结构表征
汪世龙, 张蕤, 胡源
高等学校化学学报,2005,26(11):2173~2175,-0001,():
-1年11月30日
The polyacrylamide(PAM)/α-zirconium phosphate(α-ZrP)nanocomposite was synthesized by in situ intercalative polymerization of acrylamide monomer with α-ZrP exfoliated by methylamine. Its structure and morphology were characterized by X-ray diffraction(XRD)and transmission electron microscopy(TEM). The results demonstrated that the interlayer distance of α-ZrP was expanded efectively after the polymerization of acrylamide, even forming exfoliated nan ocomposite. Both XRD and TEM image confirm ed that the most of the layers of α-ZrP were dispersed well in the polymer matrix. and the PAM/α-ZrP intercalated nanocompo site with the d-spacing of about 1.5nm can be obtained at a hish loading of α-ZrP (mass fraction≥10%). At the same time. some of the layers of α-ZrP were exfoliated an d dispe rsed disorderly in the PMA matrix.
α-磷酸锆, 聚丙烯酰胺, 原位插层-聚合, 纳米复合材料, 制备
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