利用产Nisin的乳酸链球菌WX506固态发酵豆粕，以Nisin的效价、生物量作为主要指标，考察了单因素条件对发酵的影响，并进行了正交优化，确定了一个合适配方，发酵粕的生物量达到1.03g/kg，Nisin效价达到997IU/g。

以蛋白酶、甘露聚糖酶为主的复合酶能对法夫酵母进行有效破壁，向1L发酵72h后的发酵液中加入100mg的复合酶，38℃恒温酶解72h，能使酵母破壁率接近90%。酶解时分别用化学渗透剂甘氨酸、吐温-80与复合酶结合使用，对类胡萝卜素总量几乎没有影响；但酵母破壁率有所下降。添加渗透剂未能有效提高破壁率。

采用贮藏试验对碱法破壁酵母类胡萝卜素的稳定性进行了研究。结果表明，不同贮藏条件下，酵母色素连续以两个速度常数不同的一级方程进行降解（K1>K2）。第一个一级反应主要反映受损脂肪粒中类胡萝卜素的变化，第二个反应主要反映未受损脂肪粒中类胡萝卜素的变化。比较不同贮藏条件下的试验结果及各个动力学方程的速度常数，表明采用低温、避光、隔氧及添加抗氧化剂有利于提高酵母色素的存留率，而酵母水分（<12%）及矿物盐对之影响不大。

文章主要介绍江南大学食品科学与工程学科本科综合实验的方式、内容、管理与进一步深化的几点思考。

本文用流变学实验与差热扫描量热法（Differential Scanning Calorimetry, DSC）实验研究了酵母葡聚糖与K-卡拉胶之间的交互作用。线性粘弹范围内的小幅振荡粘弹实验表明，浓度（以固形物含量计）5%以下的单独酵母葡聚糖悬浊液在实验所测条件下都表现稀液体的性质，当添加少量（浓度≤0.8%）卡拉胶后体系即表现出胶凝特性，且两相体系具有很好的相容性。当体系中酵母葡聚糖浓度为卡拉胶的10倍时，体系依然表现出类似于单独卡拉胶溶液的性质，而G’，G”都有小幅增加。从而推测卡拉胶对体系凝胶特性起主导作用，酵母葡聚糖的存在没有阻碍卡拉胶有序结构的形成，反而增强了网络体系的稳定性。混合物体系的DSC曲线表现出不同程度的吸、放热峰，但所成峰形都较宽，有别于单独卡拉胶的情况，推测体系间发生了一定的交互作用。

The protective effects of hen egg yolk phosvitin phosphopeptides (PPPs) against hydrogen peroxide (H2O2)-induced oxidative stress were evaluated in an in vitro assay using human intestinal epithelial cells. Caco-2 cells were stimulated with 1 mM H2O2 for 6 h, and the secretion of IL-8, a proinflammatory mediator, was determined by ELISA as a biomarker of oxidative stress. The inhibition of H2O2-induced IL-8 secretion from Caco-2 cells was observed by pretreatment for 2 h with PPPs, but not with phosvitin. PPPs also suppressed the formation of malondialdehyde in H2O2-treated Caco-2 cells. Furthermore, intracellular glutathione levels and glutathione reductase activity were elevated by the addition of PPPs. The protective effects of PPPs against H2O2-induced oxidative stress were almost the same as that of glutathione, and PPPs with a high content of phosphorus exhibited higher protective activity than PPPs without phosphorus; however, phosphoserine itself did not show any significant antioxidative stress activity. These findings suggest that oligophosphopeptides from hen egg yolk phosvitin possess novel antioxidative activity against oxidative stress in intestinal epithelial cells and that phosphorus and peptide structure seem to have a key role in the activity.

本文用碱法，碱酸法和酶碱法从啤酒废酵母中提取碱不溶性葡聚糖，对产品进行了定性定量分析，得到的产品主要为β-D-葡聚糖，且不含甘露糖。研究了三种产品对真菌毒素玉米赤霉烯酮（ZEA）的吸附作用，实验结果表明，酶碱法提取的葡聚糖具有较好的吸附效果，在反应条件：β-D-葡聚糖100μg/ml，玉米赤霉烯酮40μg/ml，37℃，200r/min振荡2h，吸附量最大可达到2.296μg/mg葡聚糖。

本试验研究了在经膨化处理的陈化早籼糙米日粮中添加外源a-淀粉酶和糖化酶对断奶仔猪小肠黏膜二糖酶、血液生化指标和生产性能的影响。试验为日粮不同加工处理（膨化、不膨化）和加外源酶（添加、不添加）两因子设计，共4个处理。结果表明，试验猪的生产性能总体趋势是非膨化组好于膨化组，非膨化加酶组的日增重比膨化加酶组在第14天和第28天分别高出23.66%和16.83%，料肉质量比降低了16.88%和7.36%；膨化和添加外源酶显著提高了断奶仔猪试验第14天十二指肠中麦芽糖酶的活性，然而膨化显著降低了断奶仔猪试验第28天空肠中乳糖酶、麦芽糖酶、异麦芽糖酶和蔗糖酶的活性，添加外源酶显著提高了断奶仔猪试验第28天空肠中乳糖酶和麦芽糖酶的活性。试验结果表明，对断奶仔猪高糊化度膨化陈化糙米是没有必要的。

Inulooligosaccharide (IOS) production from inulin was studied using a partially purified endoinulinase and a purified endoinulinase, which originated from Aspergillus ficuum. At the optimal conditions, including 50g/L inulin, an enzyme concentration of 10U/g substrate, 45C, and pH 6.0, the inulin-degrading degree by partially purified endoinulinase was 74% and an IOS yield over 50% were observed after 72h. The major products were identified as DP2 to DP4. The purified Endo-I was used for inulo-oligosaccharide production at the optimal conditions obtained with orthogonal experiments, including pH 5.0, 45C, 50g/L inulin, and an enzyme concentration of 10U/g substrate. With pure inulin as substrate, the maximum inulin-hydrolyzing degree was 75% and the total IOS yield was 70% after 72h. The hydrolysis products consisted of DP2 to DP8 with HPLC, and DP3 and DP4 were relatively high. With Jerusalem artichoke juice as substrate, the inulin-ydrolyzing extent reached 89% and the maximum IOS production was up to 80% after 72h. Various IOS with different DP (mainly DP2, DP3, DP4, DP5, DP7, DP8) were evenly distributed in the final reaction products.

建立了一种快速测定饲料耐水性的方法。由崩解仪加快饲料在水中的渍散，然后用浊度仪测其相对浊度比。试验结果表明：相对浊度比能全面反映饲料的耐水性能，与传统测试方法的相关系数为r=0.9678；测试时间由传统测定所需的7～8h缩短为半小时；检测重现性较好（不同样品测定的变异系数小于5%）。本测试方法快速、操作简便、成本低，适于推广使用。