毛忠贵
生物工业清洁生产技术;生物能源;生物活性物质的开发。
个性化签名
- 姓名:毛忠贵
- 目前身份:
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学术头衔:
博士生导师
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学科领域:
生物化学工程
- 研究兴趣:生物工业清洁生产技术;生物能源;生物活性物质的开发。
毛忠贵,1954年3月出生,江南大学生物工程学院教授,博士生导师。中国发酵工业协会常务理事、中国化工学会生物化工专业委员会委员、江苏省生物技术协会常务理事。研究领域:生物工业清洁生产技术;生物能源;生物活性物质的开发。近几年先后承担省部级和市级及企业委托科研项目数十项,其中,“闭路循环工艺在味精和环氧树脂生产中的应用”获中国石油和化工科技进步三等奖(2005.10)。其他奖励:1993-1995年度无锡市优秀教育工作者;1996-1997年度中国轻工总会先进个人;江苏省“333”工程第三层次培养人选;1998年教育部优秀青年教师基金资助人选;2000年教育部骨干教师基金资助人选。
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1907
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0
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成果阅读
577
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成果数
15
【期刊论文】On-line optimization of glutamate production based on balanced metabolic control by RQ
毛忠贵, Jie Xiao, Zhongping Shi, Pei Gao, Haojie Feng, Zuoying Duan, Zhonggui Mao
Bioprocess Biosyst Eng (2006) 29: 109-117,-0001,():
-1年11月30日
In glutamate fermentations by Corynebacterium glutamicum, higher glutamate concentration could be achieved by constantly controlling dissolved oxygen concentration (DO) at a lower level; however, by-product lactate also severely accumulated. The results of analyzing activities changes of the two key enzymes, glutamate and lactate dehydrogenases involved with the fermentation, and the entire metabolic network flux analysis showed that the lactate overproduction was because the metabolic flux in TCA cycle was too low to balance the glucose glycolysis rate. As a result, the respiratory quotient (RQ) adaptive control based "balanced metabolic control" (BMC) strategy was proposed and used to regulate the TCA metabolic flux rate at an appropriate level to achieve the metabolic balance among glycolysis, glutamate synthesis, and TCA metabolic flux. Compared with the best results of various DO constant controls, the BMC strategy increased the maximal glutamate concentration by about 15% and almost completely repressed the lactate accumulation with competitively high glutamate productivity.
Glutamate fermentation, Intracellular enzymes activities, Metabolic balancing, Metabolic flux analysis•On-line optimization
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【期刊论文】Electrotransformation of Thermoanaerobacter ethanolicus JW200
毛忠贵, Hui Peng, Bailing Fu, Zhonggui Mao, Weilan Shao
Biotechnol Lett (2006) 28: 1913-1917,-0001,():
-1年11月30日
Electrotransformation of Thermoanaerobacter ethanolicus JW200 was achieved using the plasmid, pTE16, and a pUC-based suicide vector, pTEA2. The construct pTE16 is based on the Escherichia coli-Clostridium perfringens shuttle vector pJIR715 and contains a thermostable chloramphenicol (Cm) resistance cassette. Evidence supporting transformation was provided by extracting plasmid pTE16 from presumptive transformants of T. ethanolicus and by PCR specific to the chloramphenicol acetyltransferase (cat) gene on the vector pTEA2. Transformation frequencies of plasmid pTE16 and pTEA2 were 50
electrotransformation, protoplast, shuttle vector, Thermoanaerobacter ethanolicus
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【期刊论文】Performance evaluation of acetone-butanol continuous flash extractive fermentation process
毛忠贵, Zhongping Shi, Chengyan Zhang, Jianxin Chen, Zhonggui Mao
Bioprocess Biosyst Eng (2005) 27: 175-183,-0001,():
-1年11月30日
A general mathematical model for performance evaluation of acetone-butanol continuous flash extractive fermentation system was formulated in the terms of productivity, energy requirement (energy utilization efficiency) and product purity. The simulation results based on experimental data showed that the most pronounced performance improvement could be achieved by using highly concentrated substrate as feed and the increase in solvent dilution rate could only improve the total productivity at the expense of energy utilization efficiency. A two-vessel partial flash system, with the first vessel of two to three plates and the second vessel as a complete flash vessel, is required to ensure high product purity. This method of mathematical formulation and analysis could be used and extended as a general tool to other bioprocesses equipped with multiple in siturecovery systems.
Acetone-butanol continuous fermentation, Computer simulation, Extractive fermentation, Flash distillation•Performance e, v, a, l, uation
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毛忠贵, Chengyan Zhang, Zhongping Shi*, Pei Gao, Zuoying Duan, Zhonggui Mao
Biochemical Engineering Journal 25 (2005) 99-108,-0001,():
-1年11月30日
The experimental data showed that, in glutamate fermentation with strain Corynebacterium glutamicum S9114, glutamate produced in different ways with different patterns of OUR, CER and lactate accumulation at different DO levels. A metabolic network model combined with the linear programming optimization was thus developed to on-line predict glutamate production under different DO levels, with only OUR and CER be on-line measured. The results indicated the advantages of the metabolic network model over the traditional unstructured models, in terms of easy parameter identification, prediction accuracy, and the universal abilities. The proposed model potentially supplies an alternative way for on-line control and optimization of fermentation processes.
Fermentation, Linear programming, Metabolic network, Modeling, On-line bioprocess monitoring
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【期刊论文】Studies on water-extractable arabinoxylans during malting and brewing
毛忠贵, Yin Lia, Jian Lua, Guoxian Gub, Zhongping Shib, Zhonggui Maob, *
Food Chemistry 93 (2005) 33-38,-0001,():
-1年11月30日
The most important non-starch polysaccharides in barley grain are b-glucan and arabinoxylans. Arabinoxylans are partially water-extractable, high-molecular-weight polymers that contribute to viscosity and membrane filterability. Changes to arabinoxylans content and endo-xylanase activity during germination and kilning were studied. This paper was aimed at etermining the composition of total arabinoxylans and water-extractable arabinoxylans in malt and observing their changes during brewing. The water-extractable and total arabinoxylans contents of six barley malts and wheat malt varied in the range of 0.42-0.98% and 3.84-6.90%, respectively. Their L-arabinose-to-D-xylose ratio ranged between 0.49-0.59 (total arabinoxylans) and 0.60-0.85 (water-extractable arabinoxylans). Contents of arabinoxylans in worts mainly originated from the soluble part of arabinoxylans in malt. The use of rice adjunct decreased arabinoxylans contents of wort and beer. The correlation between arabinoxylans and viscosity was investigated. Results showed that water-extractable arabinoxylans in wort have positive and higher correlation value with wort viscosity than those of b-glucan in wort, and both water-extractable arabinoxylans and b-glucan in beer have significant correlation (p<0.01) with beer viscosity.
Arabinoxylans, Malt, Wort, Beer
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毛忠贵, Xianghe Menga, Dongya Zoua, Zhongping Shia, *, Zuoying Duana, and Zhonggui Maoa, b
Lipids, Vol. 39 no.1 (2004),-0001,():
-1年11月30日
The inhibitory effects of 1,3-diacylglycerol (DAG) on diet-induced lipid accumulation in liver and abdominal adipose tissue of rats were investigated in the present study. Male Sprague-Dawley rats were given free access to diets containing 7 wt% TAG (low TAG), 20 wt% TAG (high TAG), or 20 wt% DAG (high DAG), respectively, for 8 wk. The body weight of rats in the 20% high-TAG group increased significantly, and the weights of their abdominal adipose tissue and liver also showed a significant increase compared with rats in the low-TAG group. However, the high-DAG diet resulted in both a significant reduction in body weight gain (with a decrease of 70.5%) and an increase in the ratio of abdominal fat to body weight (by 127%) compared with the high-TAG diet. As well, the liver TAG and serum TAG levels of the high-DAG group were significantly lower than those of the high-TAG group. These effects were associated with up-regulation of acyl-CoA carnitine acyltransferase (ACAT) and down-regulation of acyl-CoA DAG acyltransferase (DGAT) in the liver. However, no significant difference was observed in the activities of alanine aminotransferase and aspartate aminotransferase among the groups (P>0.05). The present results indicate that dietary DAG reduced fat accumulation in viscera and body, and these effects may be involved with up-regulation of ACAT and down-regulation of DGAT in the liver.
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毛忠贵, Yin Li, Jian Lu, *, Guoxian Gu, Zhongping Shi & Zhonggui Mao
Biotechnology Letters 26: 779-785, 2004.,-0001,():
-1年11月30日
A mathematical model describing the degradation of arabinoxylans by endo-xylanase during mashing process was developed. Endo-xylanase activities and arabinoxylans concentrations in laboratory scale mashing process at different temperature profiles were measured and then used for identifying the model parameters for Harrington barley malt. The modeling errors range for the final concentration of arabinoxylans in wort was -4% to +11.9%. The model developed was also used for predicting the other three different malts mashing processes in laboratory scale, and the prediction errors ranged from -9.5% to +13.6%. The model prediction accuracy for industrial scale mashing process was lower than that in laboratory scale. The simulation results showed that, a lower concentration of arabinoxylans could be achieved when maintaining the mashing-in at 45℃ and prolonging the mashing-in time.
arabinoxylans,, endo-xylanase,, mashing,, malts model,, prediction
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毛忠贵, Yibo Zhu, Jianhua Zhang, Zhongping Shi, and Zhonggui Mao
J. Inst. Brew. 110 (2), 117-123, 2004,-0001,():
-1年11月30日
The rice heat blast process is a novel technique for gelatinizing raw starch. It uses heated air to replace steam for processing rice under high temperature fluidization in a short time period. This is a new technique for making rice wine with the characteristics of easy storage and zero water pollution. This study focused on three major performance indexes (starch gelatinization ratio, total fat content, and amino nitrogen content in the roasted rice), which largely affect the performance of the rice heat blast process and the rice wine quality. The relationship between these performance indexes and the corresponding operation variables were modeled by an artificial neural network (ANN) via learning sets of experimental data. Based on the ANN models obtained, genetic algorithms were used to optimize the operating conditions of the rice heat blast process. The results showed the power in determination of optimal operating conditions by the combinational utilization of artificial neural network and genetic algorithms.
Artificial neural network,, genetic algorithm,, optimization,, quality control,, rice heat blast process.,
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毛忠贵, Xin Lu, Yongfei Li, Zuoying Duan, Zhongping Shi* & Zhonggui Mao
Biotechnology Letters 25: 1819-1826, 2003.,-0001,():
-1年11月30日
Using Saccharomyces cerevisiae, a novel, repeated fed-batch ethanol production system from corn flour by fully recycling fermented supernatants is demonstrated. With recovery of ethanol by evaporation coupled with consecutive removal of the insoluble and soluble inhibitory substances accumulated, either completely or partially by filtration, the concentrations of the soluble inhibitors in the system could be maintained at their equilibria. As a result, a sustained high concentration of ethanol (up to 15% v/v) and significant pollution control performance were obtained.
ethanol production,, minimum wastewater discharge,, repeated fed-batch,, Saccharomyces,, soluble inhibitors,, supernatant recycle
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毛忠贵, 张建华, 段作营, 李永飞
研究报告,31~34,-0001,():
-1年11月30日
研究了酒精蒸馏废液全循环对玉米粉液化、糖化和发酵的影响,结果表明,当液化酶用量达到14U/g、糖化酶用量为120U/g(玉米粉)时,蒸馏废液对液化无影响,而对糖化有促进作用;蒸馏废液能一定程度地促进酵母的生长,提高酵母耗糖速率;蒸馏废液经过25次循环发酵,酒精平均浓度在15.07%,平均糖利用率91.7%。蒸馏废液全循环工艺,解决了发酵法酒精生产中的污染问题,实现了发酵法酒精清洁生产。
酒精发酵,, 蒸馏废液,, 循环,, 清洁生产
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