堵国成
博士研究生 教授
江南大学 生物工程学院
研究领域:发酵过程优化与控制,代谢工程技术,酶工程与技术。
个性化签名
- 姓名:堵国成
- 目前身份:在职研究人员
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:高级-教授
-
学科领域:
发酵工程
- 研究兴趣:研究领域:发酵过程优化与控制,代谢工程技术,酶工程与技术。
堵国成,男,1965年10月出生,1997年12月获无锡轻工业学院发酵工程专业博士学位,2000年6月-2001年6月,香港科技大学博士后;2003年7月-2004年12月,美国夏威夷大学进行高级访问学者。目前担任Journal of the Science of Food & Agriculture (JSFA) 副主编。8种国际期刊审稿人,6种中文期刊的审稿人。
研究领域:发酵过程优化与控制,代谢工程技术,酶工程与技术。近5年来主持或完成国家自然科学基金、科技部中小企业创新基金、省自然科学基金和省“十五”科技攻关,省国际合作项目等项目15项,6项成果被鉴定达到国内领先或国际先进水平。拥有15项发明专利获授权,申请发明专利18项。近5年在发表论文140篇(SCI收录70篇)。完成专著5部。2000-2005年5项科研成果分别获省、部级科技进步一、二、三等奖,2006年1项成果获国家科技进步二等奖(排名第二),另1项获中石化科技进步二等奖。1项教学成果获国家级教学成果二等奖(排名第三)、2项教学成果获省教学成果一、二等奖。
-
主页访问
2698
-
关注数
0
-
成果阅读
361
-
成果数
8
【期刊论文】Effects of glycerol on the production of poly(g-glutamic acid) by Bacillus licheniformis
堵国成, Guocheng Dua, Ge Yanga, Yinbo Qub, Jian Chena, *, Shiyi Luna
Process Biochemistry 40(2005)2143-2147,-0001,():
-1年11月30日
The effect of glycerol on poly(g-glutamic acid) (g-PGA) fermentation was studied. The addition of glycerol as a co-substrate with Lglutamic acid and citric acid enhanced the production of g-PGA. The investigation on phospholipid composition of the cell membrane showed that, among the five major phospholipids including phosphatidylethanolamine (PE), phosphatidyl serine (PS), phosphatidic acid (PA), cardiolipin (CL) and phosphatidyl glycerol (PG), the syntheses of PG, PE, PS and CL were greatly influenced by the addition of glycerol. The further investigation of phospholipids ester-linked fatty acids showed that the existence of glycerol in medium caused the obvious decrease in C16:1 and C18:1 fatty acids and the increase in C10:0 and C12:0 fatty acids. It is concluded that the addition of glycerol led to an effective excretion of g-PGA resulting from the influences on the synthesis of cell membrane phospholipids and their ester-linked fatty acids. # 2004 Elsevier Ltd. All rights reserved.
Poly(, g-glutamic acid), , Bacillus licheniformis, Glycerol, Phospholipid, Fatty acids, Cell membrane
-
44浏览
-
0点赞
-
0收藏
-
0分享
-
290下载
-
0评论
-
引用
堵国成, Guoliang Yana, Guocheng Dua, Yin Lia, b, Jian Chena, ∗, Jianjiang Zhongc
Process Biochemistry 40(2005)963-968,-0001,():
-1年11月30日
Dissolved oxygen (DO) concentration is one of the most important environmental factors affecting cell growth and product formation. The effect of different DO concentration produced by changing the agitation speed or aeration rate the on batch production of microbial transglutaminase (MTG) with Streptoverticillium mobaraense was studied. Based on the kinetic analysis of the batch fermentation process, a two-stage agitation speed control strategy was developed, in which the agitation speed was controlled at 450 rpm in the first 24 h to achieve a DO level of up to 35%, and then switched to 350 rpm after 24 h to maintain a DO level at 15-25%. By applying this two-stage agitation speed control strategy in MTG fermentation, the maximal MTG activity and productivity reached 3.32 U/ml and 83.0 U/(l h), respectively, which were 15.3% higher than the best results in which the agitation speed was controlled at 350 rpm (2.88 U/ml and 72.0U/(lh)).
Streptoverticillium mobaraense, Microbial transglutaminase, Batch fermentation, Two-stage agitation speed control strategy
-
70浏览
-
0点赞
-
0收藏
-
0分享
-
433下载
-
0评论
-
引用
堵国成, YAN Qun, DU Guocheng and CHEN Jian
Journal of Chemical Industry and Engineering (China), 2002, 53: 104-106,-0001,():
-1年11月30日
Acetic acid, propionic acid, lactic acid, butyric acid, and a kind of the mixed four were used as carbon sources for Ralstonia eutropha to biosynthesize polyhydroxyalkanoates (PHAs) in this study. Lactic acid was used up the first among the four organic acids, acetic acid, butyric acid and propionic acid subsequently. Furthermore, acetic acid was produced when propionic acid was used as carbon source for the biosynthesis of PHAs by Ralstonia eutropha, and which was never reported before. In the fed-batch culture, the mole rate of HV component of the PHAs were relatively lower than that of the batch culture, however, the maximum yield of dry cell weight (DCW) and PHAs increased.
PHAs,, polyhydroxyalkanoates,, organic acids,, Ralstonia eutropha,, biosynthesis Abbreviations Hac: acetic acid, HLa, lactic acid, HPr, propionic acid, HBu, butyric acid
-
34浏览
-
0点赞
-
0收藏
-
0分享
-
45下载
-
0评论
-
引用
堵国成, Guocheng Du, Jinju Geng, Jian Chen, , * and Shiyi Lun
World Journal of Microbiology & Biotechnology 19: 433-437, 2003.,-0001,():
-1年11月30日
Denitrifying bacteria,, mixed culture,, nitrifying bacteria,, nitrogen compound,, simultaneous nitrification and denitrification
-
42浏览
-
0点赞
-
0收藏
-
0分享
-
458下载
-
0评论
-
引用
堵国成, 耿金菊, 陈坚
无锡轻工大学学报,2002,21(21)5:515~518,-0001,():
-1年11月30日
筛选和分离得到的多株脱氮微生物,能在完全好氧条件下将氨氮转化为NO2-,随即在好氧反硝化菌的作用下还原为N排放,整个生物脱氮过程历时较短,30h内对200mg/L的氨氮去除率达99%,而且无中间产物NO2-的积累。混合脱氮微生物菌群生长的适宜pH范围为7~10,探索实现混合脱氮微生物菌群高密度培养的pH: 发酵前期补酸控制pH≤8,发酵中后期不控制pH值,可缩短菌体的生长周期,提高菌体的氨氮降解速率。细胞质量浓度达3.9g/L,比自然pH条件下提高了62.5%.
脱氮微生物, 硝化—反硝化, 好氧脱氮, 混合培养
-
57浏览
-
0点赞
-
0收藏
-
0分享
-
238下载
-
0评论
-
引用
堵国成, Guocheng Dua, Jian Chena, Jian Yub, *, Shiyi Luna
Journal of Biotechnology 88(2001)59-65,-0001,():
-1年11月30日
Poly-3-hydroxybutyrate (PHB) is mainly accumulated by Ralstonia eutropha under unbalanced growth conditions, which limits its production in batch or fed-batch modes. The continuous production of PHB was investigated in a two-stage continuous culture system. The first-stage produced cell mass giving the maximal cell dry weight of 27.1gl-1 at 0.21h-1 of dilution rate. High specific cell growth rate results in the decrease of PHB synthesis under glucose-limited and nitrogen-rich conditions in the first-stage. The second-stage produced PHB giving the maximal PHB concentration of 47.6gl-1 at 0.14h-1 of dilution rate. Specific PHB synthetic rate reached highest value at low dilution rate under nitrogen-limited condition in the second-stage, and decreased with the increase of ammonium concentration in the culture. In the continuous culture system, the maximal PHB productivity could reach 1.43gl-1h-1 at a dilution rate of 0.12h-1, but with relatively low PHB content of 47.6%. Maximal yield of PHB on glucose could reach 0.36gg-1 glucose at 0.075h-1 of dilution rate with relatively high PHB productivity of 1.23gl-1h-1 and PHB content of 72.1%, respectively.
Ralstonia eutropha, Poly-3-hydroxybutyrate, Two-stage continuous culture system, PHB continuous production, Dilution rate, Nitrogen-limited condition
-
33浏览
-
0点赞
-
0收藏
-
0分享
-
38下载
-
0评论
-
引用
堵国成, Guocheng C. Dua, Jian Chena, Jian Yu, ∗, Shiyi Luna
Biochemical Engineering Journal 8(2001)103-110,-0001,():
-1年11月30日
The effects of propionic acid feeding strategy on production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] were studied with Ralstonia eutropha. Flask culture revealed that the time and concentration of propionic acid addition had significant effects on cell growth, P(3HB-co-3HV) synthesis, and HV fraction in the copolymer. In fed-batch culture, a low ratio of propionic acid to glucose (P/G) led to high dry cell weight (DCW), P(3HB-co-3HV) content and productivity, but low HV unit fraction. A high P/G ratio led to, on the other hand, high HV unit fraction but the low P(3HB-co-3HV) content and productivity. The specific P(3HB-co-3HV) synthetic rate and the specific HV synthetic rate declined in fed-batch cultures, which deteriorated with high P/G feeding due to the inhibitory effect of propionic acid accumulated in the culture broth. According to the non-steady state synthesis of P式(3HB-co-3HV) by R. eutropha, an optimal feeding strategy to control the propionic acid accumulationwas developed and demonstrated. The propionic acid feeding ratewas reduced with time, and DCW, P(3HB-co-3HV) concentration, P(3HB-co-3HV) content and HV fraction in P(3HB-co-3HV) reached 52.1gl-1, 40.8 gl-1, 78.3% and 16.2mol%, respectively. P(3HB-co-3HV) productivity was 0.74gl-1h-1.
Ralstonia eutropha, Poly(, 3-hydroxybutyrate-co-3-hydroxyvalerate), , Poly-3-hydroxyalkanoates, Feeding strategy, Fed-batch culture, Propionic acid
-
38浏览
-
0点赞
-
0收藏
-
0分享
-
257下载
-
0评论
-
引用
堵国成, Guo-Cheng Du, Jian Chen*, Hai-Jun Gao, Yin-Guang Chen and Shi-Yi Lun
World Journal of Microbiology & Biotechnology 16: 9-13, 2000,-0001,():
-1年11月30日
Alcaligenes eutrophus,, cell growth,, environmental conditions,, poly-b-hydroxybutyrate
-
43浏览
-
0点赞
-
0收藏
-
0分享
-
39下载
-
0评论
-
引用