罗建平
主要从事药用、食用植物生物技术的研究。
个性化签名
- 姓名:罗建平
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
细胞生物学
- 研究兴趣:主要从事药用、食用植物生物技术的研究。
罗建平,1990-1993年在中国科学院昆明植物研究所攻读植物学专业硕士学位,1995-1998年在兰州大学攻读细胞生物学专业博士学位,1998-2000年在中国科学技术大学生物学博士后流动站做博士后工作,2000年至今在合肥工业大学工作。现为合肥工业大学教授,博士生导师。主要从事药用、食用植物生物技术的研究。作为学科带头人先后参与主持申报获批植物学和食品科学硕士点、农产品加工与贮藏工程博士点和食品科学与工程一级学科博士授权点。曾获兰州大学优秀博士学位论文、安徽省自然科学优秀论文一等奖、安徽省科技进步三等奖、陕西省高等学校科学技术一等奖基金。先后主持多项国家及省自然科学基金、博士点基金及创新团队建设项目。现担任安徽省生物化学与分子生物学会理事和安徽省细胞生物学会常务理事。
在植物细胞与器官反应器培养实现药用植物资源持续开发和高附加值产品生物合成研究方面,先后建立了人参、红豆杉、怀槐、石斛等名贵药用植物细胞与器官反应器大量培养高效表达体系,完成人参和红豆杉细胞500升反应器试验、发酵细胞生产活性组分的制备工艺与含量检测标准,运用神经网络结合遗传算法实现植物细胞对活性组分的高效合成,优化出反应器运行的关键控制参数和生物合成的反应动力学模型;在国际上首例报告红豆杉属植物原生质体培养成功,并进行了高产细胞的分子诱变分离与长期冰冻保存研究,为反应器发酵生产紫杉醇准备了优良细胞系;在固定化细胞反应器中生物转化植物异黄酮糖苷为活性苷元的研究上取得明显进展。
在植物离体形态建成及调控机理研究方面,选择资源濒临灭绝的药用石斛为对象,先后建立起霍山石斛等9种药用石斛的类原球茎再生植株的繁育体系,实现了类原球茎试管种质的长期保存,对再生能力和遗传稳定性进行了较系统的评价,实现了类原球茎在无外源激素下的离体形态建成;从形态、组织、细胞、染色体及分子水平上系统研究了豆科植物细胞与原生质体的形态发生途径及发育调控机理,分析了T-DNA插入调控植物体细胞胚胎发生的效应及胚性相关基因的标记定位,发现信号分子水杨酸对植物体细胞胚胎发生的促进作用和水杨酸激发的活性氧信号相关,提出细胞通过活性氧偶联抗性反应和胚胎发生的新观点,并证实低温促进的体细胞胚胎发生与细胞内的钙信号转导相关,研究结果在SCI收录刊物上发表并被他人引用20余次,研究论文获得2004年安徽省自然科学优秀论文一等奖。
在药用植物次生代谢途径调控及转基因研究方面,以红豆杉和怀槐细胞为对象,系统分析了碳、氮代谢关键酶表达、细胞信号转导、细胞超微结构发育对次生代谢途径相关酶表达的调控,较深入探讨了莽草酸途径关键酶同工酶的差别表达对植物次级代谢途径前体物质的分流与调节,开展了异黄酮生物合成关键酶基因克隆及其表达与DNA甲基化关系的研究,并对药用植物千层塔石杉碱生物合成的遗传与环境互作机制进行了研究,成功实现了T-DNA对红豆杉原生质体的转化,目前正在开展异黄酮合成关键酶异黄酮合酶基因的转化与表达调控研究。近年在核心期刊发表学术论文80余篇,其中SCI收录10余篇,国家发明专利公开1项。
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336
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成果数
9
罗建平, Jian-Ping Luo*, Xue-Qiang Zha & Wei Shi
Plant Cell, Tissue and Organ Culture (2005) 82: 75-81,-0001,():
-1年11月30日
In vitro selection was carried out to obtain ethionine-resistant plants with increased contents of free methionine in the vegetative tissues of the forage legume Astragalus adsurgens Pall. Three-week-old cell colonies were derived from protoplasts mutagenized with N-methyl-N-nitrosoguanidine from embryogenic callus and were selected with 0.6 mM ethionine. Four colony lines were isolated and their resistance to ethionine was 7-8 times that of the wild-type callus. No plant regeneration occurred on these colony lines in the differentiation medium containing ethionine. Only one colony line (R-1) regenerated plants through somatic embryogenesis in the absence of ethionine. Stem and leaf segments from the regenerated plants showed the same potential to produce callus in the presence of ethionine as in the absence of ethionine. The formed callus kept continuously growing in ethionine-containing medium. Free amino acid analysis revealed that colony line R-1, its regenerated plants and callus from the regenerated plants accumulated methionine at levels at 5-9 times higher than in wild-type. These results suggested that ethionine resistance and methionine over-accumulation were also expressed at plant level. Thus, the obtained resistant colony line that could regenerate plants with over-accumulation of methionine might provide an alternative approach to improve the nutritional quality of this forage.
Astragalus adsurgens Pall,, ethionine-resistance,, methionine,, plant regeneration,, protoplasts
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罗建平, Jian-Ping Luo*, Shao-Tong Jiang and Li-Jun Pan
Plant Growth Regulation 40: 171-177, 2003,-0001,():
-1年11月30日
The inter-relationship between exogenous calcium (Ca2+) during cold pretreatment and cold-enhanced somatic embryogenesis was investigated using cell suspension cultures of Astragalus adsurgens Pall. Cell suspension was obtained from embryogenic callus and could be induced to form somatic embryos in the differentiation medium. Suspension cells, after cold-treatment at 8℃ for 2 to 3 wk, displayed an enhanced capacity for somatic embryogenesis as compared to those without cold pretreatment. Longer cold pretreatment (>4wk) resulted in the inhibition of somatic embryogenesis. The enhanced embryogenic response of cells to cold pretreatment was dependent on the Ca2+ level in the pretreatment medium. Ca2+ levels below 1 mM suppressed the cold-enhanced response. Addition of lanthanum into the pretreatment medium completely abolished the cold induced enhancement of somatic embryogenesis. These results suggest that embryogenic cells require a minimal concentration of Ca2+ during pretreatment for the expression of this cold-enhanced capacity for somatic embryogenesis in A.adsurgens and the influx of exogenous Ca2+ during pretreatment might also be involved.
Astragalus adsurgens,, Calcium,, Cold pretreatment,, Somatic embryogenesis
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罗建平, Jian-Ping Luo*, Xue-Qiang Zha & Wei Shi
Plant Cell, Tissue and Organ Culture (2005)82: 75-81,-0001,():
-1年11月30日
In vitro selection was carried out to obtain ethionine-resistant plants with increased contents of free methionine in the vegetative tissues of the forage legume Astragalus adsurgens Pall. Three-week-old cell colonies were derived from protoplasts mutagenized with N-methyl-N-nitrosoguanidine from embryogenic callus and were selected with 0.6mM ethionine. Four colony lines were isolated and their resistance to ethionine was 7-8 times that of the wild-type callus. No plant regeneration occurred on these colony lines in the differentiation medium containing ethionine. Only one colony line (R-1) regenerated plants through somatic embryogenesis in the absence of ethionine. Stem and leaf segments from the regenerated plants showed the same potential to produce callus in the presence of ethionine as in the absence of ethionine. The formed callus kept continuously growing in ethionine-containing medium. Free amino acid analysis revealed that colony line R-1, its regenerated plants and callus from the regenerated plants accumulated methionine at levels at 5-9 times higher than in wild-type. These results suggested that ethionine resistance and methionine over-accumulation were also expressed at plant level. Thus, the obtained resistant colony line that could regenerate plants with over-accumulation of methionine might provide an alternative approach to improve the nutritional quality of this forage.
Astragalus adsurgens Pall,, ethionine-resistance,, methionine,, plant regeneration,, protoplasts
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罗建平, Jian-Ping Luo*, Shao-Tong Jiang and Li-Jun Pan
Plant Growth Regulation 40: 171-177, 2003.,-0001,():
-1年11月30日
The inter-relationship between exogenous calcium (Ca2+) during cold pretreatment and cold-enhanced somatic embryogenesis was investigated using cell suspension cultures of Astragalus adsurgens Pall. Cell suspension was obtained from embryogenic callus and could be induced to form somatic embryos in the differentiation medium. Suspension cells, after cold-treatment at 8℃ for 2 to 3wk, displayed an enhanced capacity for somatic embryogenesis as compared to those without cold pretreatment. Longer cold pretreatment (>4wk) resulted in the inhibition of somatic embryogenesis. The enhanced embryogenic response of cells to cold pretreatment was dependent on the Ca2+ level in the pretreatment medium. Ca2+ levels below 1 mM suppressed the cold-enhanced response. Addition of lanthanum into the pretreatment medium completely abolished the cold induced enhancement of somatic embryogenesis. These results suggest that embryogenic cells require a minimal concentration of Ca2+ during pretreatment for the expression of this cold-enhanced capacity for somatic embryogenesis in A. adsurgens and the influx of exogenous Ca2+ during pretreatment might also be involved.
Astragalus adsurgens,, Calcium,, Cold pretreatment,, Somatic embryogenesis
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罗建平, Jian-Ping Luo*, Shao-Tong Jiang, Li-Jun Pan
J.-P. Luo et al./Plant Science 161(2001)125-132,-0001,():
-1年11月30日
Salicylic acid (SA), when added to the differentiation medium below 200mol/l, significantly enhanced somatic embryogenesis in callus culture of Astragalus adsurgens Pall. The highest frequency of somatic embryogenesis occurred at 150mol/l SA. Enhanced somatic embryogenesis by SA was accompanied by an increase in the endogenous H2O2 level as compared with controls. This increased endogenous H2O2 level was related to the inhibition of the activities of ascorbate peroxidase (APX) and catalase (CAT). Although the promoting effect of exogenous H2O2 was significantly lower than that of exogenous SA on the development of somatic embryos, the pre-treatment of callus with dimethylthiourea (a trap for H2O2) significantly inhibited somatic embryogenesis, even if callus was cultured on the differentiation medium supplemented with 150
Salicylic acid, Hydrogen peroxide (, H2O2), , Astragalus adsurgens Pall., , H2O2 metabolising enzymes, Somatic embryogenesis
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罗建平, Jian-Ping Luo a, *, Jing-Fen Jia b, Yue-Hua Gu a, Jing Liu a
J.-P. Luo et al./Plant Science 143(1999)93-99,-0001,():
-1年11月30日
Efficient plant regeneration through somatic embryogenesis was achieved in Astragalus adsurgens. Embryogenic callus was induced from hypocotyl, not root or cotyledon explants, and the maximum induction frequency (62%) was obtained on Murashige and Skoog (1962) basal medium (MS) containing 2.0 mg:l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5mg/l N6-benzylaminopurine (BA). Transfer of embryogenic calli to MS medium with 1-2mg/l BA and 0.1mg/l a-naphthaleneacetic acid (NAA) resulted in somatic embryogenesis at high frequencies (63-74%) with an average of 280 somatic embryos per gram of embryogenic callus. After transfer onto half-strength MS medium without growth regulators, approximately 80% of somatic embryos developed into complete plantlets. Chromosome count of root tips from regenerants revealed a normal diploid. The rooted plantlets were successfuly transferred to soil with 60-80% survival, and the plants showed normal morphological characteristics. On MS medium containing 1.0mg/l 2,4-D and 0.5mg/l BA, embryogenic calli have retained morphogenetic potential for 12 months.
Astragalus adsurgens, Embryogenic callus, Forage legume, Plant regeneration, Somatic embryogenesis
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【期刊论文】Protoplast culture and paclitaxel production by Taxus yunnanensis
罗建平, Jian-Ping Luo, *, QingMu & Yue-Hua Gu
Plant Cell, Tissue and Organ Culture 59: 25-29, 1999.,-0001,():
-1年11月30日
Viable protoplasts of Taxus yunnanensis were isolated from friable, light yellow callus. Protoplast yield was dependent on callus age, with a maximum from 20-day-old callus. Protoplasts were induced to undergo sustained divisions and to form cell colonies when cultured in medium consisting of B5 salts, KM vitamin and organic components, 0.45 M fructose, 3.0mg l-1 2,4-dichlorophenoxyacetic acid and 0.1 mg l-1 kinetin. The planting density was 2.5-3.0
Taxus yunnanensis,, protoplast-derived colonies,, paclitaxel
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罗建平, J.-P. Luo • J.-F. Jia
Plant Cell Reports (1998)17: 567-570,-0001,():
-1年11月30日
An efficient procedure was developed for inducing callus and plant regeneration using hypocotyls segments of Astragalus adsurgens. The combinations and concentrations of different growth regulators were shown to be critical factors for both the frequency and the type of callus formation as well as for the potential of callus differentiation. Of the four morphologically distinct types of calli that were induced, a friable, yellow callus, i.e. type I, induced on MS medium supplemented with 9.0 mM 2,4-dichlorophenoxyacetic acid and 2.2 mM N6-benzylaminopurine (BA), and then transferred to MS medium containing 0.5 mM a-naphthaleneacetic acid and 8.9 mM BA, exhibited the maximum frequency of shoot regeneration (75%). After regenerated shoots were transferred onto halfstrength MS medium without growth regulators, they rooted and complete plants were obtained. Plantlet regeneration from callus cultures required 7-8 weeks.
Callus induction • Plant regeneration • Astragalus adsurgens
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【期刊论文】Plant regeneration from callus protoplasts of the forage legume Astragalus adsurgens Pall.
罗建平, J.-P. Luo • J.-F. Jia
Plant Cell Reports (1998)17: 313-317,-0001,():
-1年11月30日
A reproducible release of viable protoplasts was obtained from friable calli of Astragalus adsurgens. Protoplasts underwent sustained divisions and formed cell colonies when cultured in either liquid or agarose-solidified Kao and Michayluk (1975) protoplast medium (KM8P) supplemented with 1.5mg/l 2,4-D, 0.5mg/l BA and 0.5 Mglucose. Compared to liquid culture, agarose bead culture improved division frequency effectively, the two culture systems showing a plating efficiency of 0.8
Astragalus adsurgens • Protoplast culture • Plant regeneration • Embryogenesis
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