高向东
长期从事生物新药的研制、多糖的结构与功能研究及衰老的分子生物学研究。
个性化签名
- 姓名:高向东
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
微生物药物学
- 研究兴趣:长期从事生物新药的研制、多糖的结构与功能研究及衰老的分子生物学研究。
高向东,女,1963年7月生,毕业于中国药科大学生物制药专业和南京大学生命科学学院植物学专业,获博士学位。1993年曾赴日本歧阜药科大学分子生物实验室作访问学者。
1983年留校任教以来,一直从事教学、科研和管理工作。现任中国药科大学微生物与生化药学教授(博士生导师)、中国药科大学生命科学与技术学院党委书记、副院长。兼任国家药品监督管理局保健食品评审委员,中国药学会生化药物专业委员会委员,中国生物化学与分子生物学会工业生物化学与分子生物学分会常务理事,中国微生物学会海洋药物委员会理事,江苏省抗衰老科学技术协会常务理事,《药物生物技术》杂志、《中国药科大学学报》编委。
长期从事生物新药的研制、多糖的结构与功能研究及衰老的分子生物学研究,已主持完成了国家863项目、国家自然科学基金项目、江苏省重点项目等国家及省部级课题15项,实现科技成果转化项目3项;申请发明专利11项(已授权6项);发表科研论文90余篇,被SCI收录15篇,包括发表在Biochimie、Biochimica et Biophysica Acta等的论文,出版专著5部。
主持完成的国家863课题“Ⅰ类抗肿瘤新药真菌多糖YCP的临床前研究”通过了科技部组织的专家验收。主持完成的江苏省自然科学基金和教育部资助项目“老年性痴呆对NGF基因表达与调控的影响及治疗药物研究”,通过了江苏省科技厅组织的专家鉴定。主持研发的“卡介菌多糖胶囊”已获国家新药证书。
荣获第四届教育部“高校青年教师奖”,江苏省“三八”红旗手称号,江苏省高校“红杉树”园丁奖,南京市千名岗位能手称号。享受政府特殊津贴。入选江苏省“333”工程培养人选、江苏省“六大人才高峰” 培养人选。承担的《生物制药工艺学》课程获江苏省一类优秀课程奖。
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201
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成果数
5
高向东, Fang Han a, Wenbing Yao a, Xiaobing Yang b, Xiaoni Liu a, Xiangdong Gao a, ∗
F. Han et al./International Journal of Biological Macromolecules 36(2005)201-207,-0001,():
-1年11月30日
ese sulfated derivates were evaluated by activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and platelet aggregation assay. The results showed that YCP sulfates significantly prolonged APTT, TT and PT. The derivates showed no effects on thrombin in the presence or in the absence of antithrombin Ⅲ (AT Ⅲ) or heparin cofactor II (HC Ⅱ), while the derivates effectively inhibited factor Xa in the presence of AT Ⅲ. At the same time, YCP-SH also possessed potent antiplatelet aggregation activity in vitro compared with aspirin. YCP sulfates specifically interfered with different stages of the coagulation cascade, and the anticoagulant activity improved with the increasing DS and decreased Mw.
Marine polysaccharide, Sulfation, Anticoagulant activity, Antiplatelet aggregation activity
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高向东, 李梅, 吕炜锋
药物生物技术, 2005, 12 (3): 162~166,-0001,():
-1年11月30日
为了实现用微生物发酵法生产辅酶Q10,筛选出一株辅酶Q10高产菌株,并对其从C源、N源、最适温度、最适pH值、通氧量、添加前体等方面进行了优化。筛选出的菌株用不同的碳源、氮源在37℃培养24h,检测其CoQ10含量;以富马酸为碳源、玉米浆为氮源、35℃、pH7.2发酵24h辅酶Q10含量达87mg/L;通氧量对该菌生长及辅酶Q10合成有促进作用;添加前体对羟基苯甲酸、异戊二烯、β2胡萝卜素均可有效提高辅酶Q10产量,其中异戊二烯可提高产率178.4%。该菌辅酶Q10产量较高,经TLC2UV法定量,HPLC法进行纯度检测,红外、质谱分析进行成品鉴定,其纯度达99.5%。
辅酶Q10, 筛选
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高向东, Cheng Sun a, Jian-Wen Wang a, Lei Fang a, Xiang-Dong Gao b, Ren-Xiang Tan a, *
C. Sun et al./Life Sciences 75(2004)1063-1073,-0001,():
-1年11月30日
The reactive oxygen species (ROS) and free radical-initiated reactions are ascertained to play multiple roles in degenerative or pathological events such as aging, cancer, heart dysfunction and Alzheimer's disease. EPS2 with a mean molecular weight of 1.3×105 was characterized as an antioxidant exopolysaccharide from the broth of a marine filamentous fungus Keissleriella sp. YS 4.08. Compositionally, it is composed of galactose, glucose, rhamnose, mannose and glucuronic acid in an approximate proportion of 50∶8∶1∶1∶0.4. The radical eliminating and antioxidant actions of the glycan was assessed in different in vitro systems showing that EPS2 exhibited profound scavenging activities in superoxide radical. As a reinforcement of the action, similar radical scavenging effects of EPS2 were also discerned with both site-specific and non site-specific hydroxyl radical using the deoxyribose assay method. Moreover, EPS2 effectively blocked as well the non site-specific strandbreaking of DNA induced by the Fenton reaction at concentrations of 0.1 and 1mg/mL. Further investigation of the effect of EPS2 on human low density lipoprotein (LDL) system demonstrated that it significantly inhibited copper-mediated oxidation of LDL in a dose-dependent manner. These results suggest that EPS2, possessing pronounced free radical scavenging and antioxidant activities, could be of considerable preventive and therapeutic significance to some life-threatening health problems such as cancer, atherogenesis and Alzheimer's disease which pathologically initiated by the presence of free radicals leading to the inevitable peroxidation of important biomolecules.
EPS2, Polysaccharide, Keissleriella sp., , Antioxidant, Free radicals
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高向东, 崔玉海, , 何成强
中国生化药物杂志,2004,25(5):278~281,-0001,():
-1年11月30日
目的建立凋亡素基因克隆及原核表达的方法。方法根据已报道的鸡贫血病毒凋亡素基因设计合成一对引物,通过PCR扩增,构建重组质粒pUC182VP3,将其与pET30质粒分别用限制性内切酶NdeⅠ、EcoRⅠ消化,构建原核表达载体pET302VP3,用以转化感受态的EcoliDE3,经IPTG化学诱导表达,表达产物进行SDS2PAGE电泳鉴定。结果凋亡素基因在EcoliDE3中获得了大量表达。结论该结果对凋亡素进一步开发为抗肿瘤新药具有重要意义。
凋亡素, 克隆, 表达, 抗肿瘤
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【期刊论文】Study on the Gene Transcription Level of Hippocampus NGF in C57 Mouse Development
高向东, Liu Xiaojun *, Wu Wutong **, Zhang Linyuan and Gao Xiangdong
Journall of Chinese Pharmaceutical Sciences 2009, 9 (2): 108-111,-0001,():
-1年11月30日
D-Nerve growth factor (13 NGF) mRNA levels in hippocampa from CsvBL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking 13-actin gene as an internal control. The levels of 6-and 12-month-old C57 mice were much lower compared with that of the 1-month-old mouse. However, there was no significant difference between these two groups. This result suggests that gene ranscription level of hippocampus 13 NGF in a C57 mouse descends evidently as it grows up. Base T at Site 294 of 13 NGF cDNA was substituted by C in the C57 mouse, as revealed by DNA sequencing for the first time. Nevertheless, this polymorphism of nucleotide did not make any difference in amino acid composition.
NGF, 13-actin, RT-PCR, Gene trans, c, r, i, p, t, ion, C57 mouse, Development
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