许金钩
主要从事分子荧光光谱学和荧光分析新技术、免疫分析、核酸荧光探针和室温敏化磷光等方面的研究工作。
个性化签名
- 姓名:许金钩
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
分析化学
- 研究兴趣:主要从事分子荧光光谱学和荧光分析新技术、免疫分析、核酸荧光探针和室温敏化磷光等方面的研究工作。
许金钩,男,1937年出生,教授,博士生导师。1958年毕业于厦门大学化学系,留校任教至今。于1964年9月-1965年10月到北京大学化学系进修,1981年2月-1983年3月到加拿大不列颠哥伦比亚大学化学系作访问学者。1978年后历任讲师、副教授、教授、分析化学教研室主任、系副主任、福建省化学会理事长。现任厦门大学化学系教授、博士生导师、中国化学会理事兼分析化学委员会委员、中国微量元素科学研究会理事、全国高等学校化学教育研究中心学术委员会副主任、教育部化学教学指导委员会无机及分析化学教学指导组成员、《分析化学》编委、山西大学化学系及安徽师范大学化学系兼职教授。
长期从事分析化学方面的教学和科研工作,主要从事分子荧光光谱学和荧光分析新技术、免疫分析、核酸荧光探针和室温敏化磷光等方面的研究工作。曾与陈国珍教授等人合编《荧光分析法》和《荧光分析进展》等论著4本,在国内外重要学术刊物上发表论文近200篇。先后主持了5项国家自然科学基金、3项国家教委博士点基金和1项福建省自然科学基金的研究课题。曾获国家教委科学科技进步二等奖一次,国家教委(或教育部)科技进步三等奖三次,国家教委优秀教材二等奖、福建省优秀教学成果二等奖、光华科技基金三等奖和福建省科技成果三等奖各一次。
主要学术成果:1、研究和建立了某些同步荧光、导致荧光和荧光总发光光谱测定的新技术和新方法。并应用三维荧光光谱和三维荧光偏振光谱探索某些环境介质的性质、蛋白质和DNA的构象。2、研究并开拓了某些非荧光物质的光化学荧光衍生化反应,建立某些光化学荧光分析新方法。3、开拓了光化学荧光探针、酞菁与花菁类红区/近红外荧光探针、SiO2-有机染料纳米微球荧光探针、基于钯-偶氮类配合物和罗丹明内酰胺衍生物的光学分子探针,并相应地建立了多种测定生物物质和环境污染物的分析新方法和新技术。4、建立了某些热敏/PH敏感高分子相分离荧光免疫分析新技术。5、制备了含磁铁矿的SiO2纳米微球,以及氧化铝模板上制备的溶胶-凝胶纳米管阵列,并应用于生物物质的分离和分析。6、开拓了氯化血红素、某些酞菁化合物和硫-铁团簇化合物作为过氧化物酶的模拟酶用于酶促分析。
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781
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成果数
12
许金钩, Shun-Hua Li, Chun-Wei Yu and Jin-Gou Xu*
Chem. Commun., 2005, 450-452,-0001,():
-1年11月30日
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许金钩, Chang-Qing Zhu a, Jin-Long Chen b, Hong Zheng a, Yu-Qin Wu b, Jin-Gou Xu a, *
Analytica Chimica Acta 539(2005)311-316,-0001,():
-1年11月30日
A new highly sensitive and selective colorimetric method for fluoride determination in water is described. The novel reagent for this method is a cyanine dye (C1) on which the hydroxyl group has been protected by reaction with tert-butyldimethylsilane (TBS) to form the silanated dye, C2. C2 is selectively attacked by fluoride ions to reform C1. C1 has an absorption maximum at 600nm with a molar absorptivity of about 200,000. Under optimized conditions, absorbance at 600nm is proportional to fluoride concentration up to about 1
Fluoride ion, Cyanine, Hydroxyl deprotection reaction, Colorimetric method
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许金钩, Peng Lin a, b, Jian-Jun Feng b, c, Hong Zheng a, Huang-Hao Yang a, Jin-Gou Xu a, *
Talanta 65(2005)430-436,-0001,():
-1年11月30日
A fluorescence immunoassay for human IgG (Ag) was developed using a pH-sensitive polymer prepared by thermal initiation or redox initiation polymerization as a carrier. In the competitive immunoassay, appropriate quantity of Ag was immobilized on the polymer and the standard Ag (or sample) solution, and a constant amount of fluorescein isothiocyanate labeled goat anti-human IgG antibody (Ab-FITC) was added. Immobilized Ag and the standard (or sample) Ag competed for binding to the Ab-FITC in 37℃ in homogeneous format. After changing the pH to separate the polymer-immune complex precipitate, it was re-dissolved and determined by fluorescence method. The results showed that the immobilization efficiency, immunological reaction activities of immobilized Ag and phase transition pH range were improved as Ag was immobilized by thermal initiation instead of redox initiation polymerization. Under optimum conditions, the calibration graphs for the Ag in both methods, thermal initiation and redox initiation, were linear over the concentration range of 0.0-1000ngmL-1, with detection limits 8 (thermal initiation) and 12ngmL-1 (redox initiation), respectively. Moreover, some pH-sensitive polymer prepared only in organic solvent or under high temperature could also be used as an immunoreaction carrier by thermal initiation polymerization. Thermal initiation polymerization was a better immobilization mode.
pH-sensitive phase separating polymer, Fluorescence immunoassay, Human IgG
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许金钩, Shun-Hua Li, Wen-Tao Yuan, Chang-Qing Zhu, and Jin-Gou Xu*
Analytical Biochemistry 331(2004)235-242,-0001,():
-1年11月30日
A new chromogenic/fluorogenic molecular probe was developed for highly selective and species-differentiable detection of phosphate-containing anions in neutral aqueous solution. The coordinatively unsaturated lanthanide complex, made from Eu(Ⅲ) ion and 2-[(8-hydroxy-5-sulfo-7-quinoline)azo]-1,8-dihydroxy-3,6-naphthalene disulfonic acid, changed its conformation when binding to the incoming target anions, which resulted in differential absorption or fluorescence responses. It was demonstrated that not only phosphate and pyrophosphate but also DNA and RNA could be clearly distinguished by visible absorption or fluorescence spectra. Also, differential responses in absorption spectra were observed when AMP, ADP, and ATP were added into the sensing system. Selective quantitation of these phosphate-containing anions in aqueous solutions, therefore, can be easily available. DNA and RNA were distinguished by different colors and independent fluorescence emissions due to their intrinsic differences in b-D-ribose residues. Simultaneous or independent quantitation of DNA and RNA in a mixture sample, therefore, is possible without pretreatment with nuclease. Furthermore, the influence from the base selectivity can be eliminated by the use of the probe. The detection limits of phosphate and 50-ATP in neutral water were 6.0×10-7 and 9.0×10-7M, respectively, by the UV/Vis spectrophotometric method; the detection limits were 12.3ng/mL for DNA by fluorimetry and 2.3mg/L for RNA by UV/Vis spectrophotometry.
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许金钩, Chang-Qing Zhu, a, b, Shu-Juan Zhuo, Hong Zheng, Jin-Long Chen, Dong-Hui Li, c, Shun-Hua Li a and Jin-Gou Xu*a
Analyst, 2004, 129, 254-258,-0001,():
-1年11月30日
A fluorescence enhancement method with a cationic cyanine as a probe was developed for the determination of nucleic acids. Under the experimental conditions, the fluorescence enhancement of cyanine (lex/lem=524/591.5nm) was observed in the presence of DNA. The calibration graphs were linear over the range of 0.01-15mg mL-1 for both calf thymus DNA (CT DNA) and fish sperm DNA (FS DNA). The limits of detection were 0.005 and 0.007mg mL-1 for CT DNA and FS DNA, respectively. The method was applied to the determination of DNA in synthetic and real samples and satisfactory results were obtained. A possible fluorescence enhancement mechanism was also studied.
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【期刊论文】Novel fluorescent silica nanoparticle probe for ultrasensitive immunoassays
许金钩, W. Yang a, C.G. Zhang b, H.Y. Qu a, H.H. Yang a, J.G. Xu a, *
Analytica Chimica Acta 503(2004)163-169,-0001,():
-1年11月30日
The preparation and utilization of a novel particulate label based on fluorescent hybrid silica (FHS) nanoparticles are reported in this article. These nanoparticles have shown several unique advantages over existing dye molecules, quantum dots, and latex-based fluorescent particles in easy preparation, good photostability and high sensitivity. A high molar ratio of the fluorescent molecules present in the core to biomolecules on the particle surface was achieved by using the well-developed silica surface immobilization chemistry for biomolecular linking. A fluoroimmunoassay method for detecting trace level Hepatitis B Surface Antigen (HBsAg) was developed. The calibration graph for HBsAg was linear over the range 0.5-220ng/ml with a detection limit of 0.1ng/ml. The sensitivity is greatly increased when compared with the corresponding immunoassay performed with direct fluorophore labeling. The present work shows that these FHS nanoparticles are high-quality markers for biochemical assays.
Fluorescent hybrid silica (, FHS), nanoparticles, Hepatitis B Surface Antigen (, HBsAg), , Fluoroimmunoassay
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许金钩, Huang-Hao Yang, Hui-Ying Qu, Peng Lin, Shun-Hua Li, Ma-Tai Ding and Jin-Gou Xu*
Analyst, 2003, 128, 462-466,-0001,():
-1年11月30日
Nanometer-sized fluorescent hybrid silica (NFHS) particles were prepared for use as sensitive and photostable fluorescent probes in biological staining and diagnostics. The first step of the synthesis involves the covalent modification of 3-aminopropyltrimethoxysilane with an organic fluorophore, such as fluorescein isothiocyanate, under N2 atmosphere for getting a fluorescent silica precursor. Then the NFHS particles, with a diameter of well below 40 nm, were prepared by controlled hydrolysis of the fluorescent silica precursor with tetramethoxysilane (TMOS) using the reverse micelle technique. The fluorophores are dispersed homogeneously in the silica network of the NFHS particles and well protected from the environmental oxygen. Furthermore, since the fluorophores are covalently bound to the silica network, there is no migration, aggregation and leakage of the fluorophores. In comparison with common single organic fluorophores, these particle probes are brighter, more stable against photobleaching and do not suffer from intermittent on/off light emission (blinking). We have used these newly developed NFHS particles as a fluorescent marker to label antibodies, using silica immobilization method, for the immunoassay of human a-fetoprotein (AFP). The detection limit of this method was down to 0.05ng mL-1 underour current experimental conditions. We think this material would attract much attention and be applied widely in biotechnology.
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许金钩, Xin-Qi Zhan a, Dong-Hui Li b, Hong Zheng b, Jin-Gou Xu b, *, Yi-Qun Zhou c
Talanta 58(2002)855-860,-0001,():
-1年11月30日
A sensitive fluorimetric method for the determination of nitrogen oxides (NOx: NO+NO2) in air is described. Nitrogen dioxide (nitrogen monoxide was previously converted to nitrogen dioxide in oxide tubes) was aspirated through a fritted glass bubble at a flow rate of 500ml min-1 for 120min and fixed as nitrite, using 0.1 N NaOH as a trapping solution with the empirical absorption efficiency 0.74 and the stoichiometric factor 0.5. The method is based on the fluorescence quenching of a red-region fluorescent reagent, tetra-substituted amino aluminum phthalocyanine (TAAlPc), after being diazotized by nitrite. Under optimal conditions the linear range of the calibration curve for nitrite is 1-40ng ml-1 (NO2 0.24-9.6 ppb, v/v). The detection limit is 0.34ng ml-1 for nitrite (NO2 0.08 ppb, v/v) and the relative standard deviation for six replicate measurements of 15ng ml-1 nitrite is 3.2%. The method has been applied to the determination of nitrogen oxides in the air with satisfactory results. Typical gaseous co-pollutants such as SO2, H2S and HCHO did not interference the determination.
Nitrogen oxides, Tetra-substituted amino aluminum phthalocyanine, Fluorimetry, Determination
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许金钩, Huang-Hao Yang, a, b Qing-Zhi Zhu, Hui-Ying Qu, Xiao-Lan Chen, Ma-Tai Ding, b, and Jin-Gou Xu a, *
Analytical Biochemistry 308(2002)71-76,-0001,():
-1年11月30日
Sol-gel-derived mesoporous biomaterials were used for the first time in the flow-injection fluorescence immunoassay system. Anti-gentamicin antibody was immobilized in a mesoporous sol-gel material using tetramethoxysilane as a precursor and poly(ethylene glycol) as a template. The sol-gel glass was used to develop an immunoaffinity column for the flow-injection immunoassay of gentamicin. Little unspecific adsorption of gentamicin on the sol-gel and no antibody leaching under harsh elution conditions were found. The immunoassay is based on the competition between gentamicin and fluorescein isothiocyanate-labeled gentamicin for a limited number of encapsulated antibody binding sites. NaOH solution of 5
Flow injection immunoassay, Sol-gel material, Gentamicin
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【期刊论文】Fluorescence Immunoassay System Based on the Use of a pH-Sensitive Phase-Separating Polymer
许金钩, Huang-Hao Yang, *, Qing-Zhi Zhu, Shi Chen, Dong-Hui Li, , Xiao-Lan Chen, Ma-tai Ding, * and Jin-Gou Xu*
,-0001,():
-1年11月30日
Poly(N-isopropylacrylamide-co-methacrylic acid) [P(NIPAAm-co-MAA)], a linear water-soluble pH-sensitive phase-separating polymer, was synthesized and used as a novel separation carrier for the reactants in immunoassay. This polymer precipitates out of water below a critical pH 5.8 at 37℃ and redissolves when the pH of solution is above 6.2. The characteristic of this polymer makes it possible to carry out the immunochemical steps of an immunoassay in a true solution and then to quickly separate the resulting product from the reaction mixture. The above approach was applied to determination of α-fetoprotein with the competitive immunoassay format. Compared with traditional ELISA using the same reactants, the proposed method was much faster (the assay time decreased from 100±120 to 30min) and showed similar sensitivity, i.e., 0.04ng/mL. In addition, a sandwich immunoassay method for the determination of hepatitis B surface antigen was also studied, and the results showed that the pH phase-separating immunoassay could be carried out through a sandwich or a competitive method. This general technique may also be used for a wide variety of separation processes in addition to immunoassay, in which a speci®c component is to be isolated for analysis, recovery, or disposal.
immunoassay, pH-sensitive phase-separating polymer, α-fetoprotein, hepatitis B surface antigen.,
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