程汉华
主要开展人及动物的细胞分子发育生物学相关领域的研究工作,如动物发育相关基因,性别决定与分化相关基因的分离克隆及作用机理的探索研究。
个性化签名
- 姓名:程汉华
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学术头衔:
博士生导师
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学科领域:
细胞生物学
- 研究兴趣:主要开展人及动物的细胞分子发育生物学相关领域的研究工作,如动物发育相关基因,性别决定与分化相关基因的分离克隆及作用机理的探索研究。
程汉华,女,教授、博士生导师。1993 年毕业于华西医科大学生物化学及分子生物学系,获博士学位,1998-1999年,在美国西东大学生物学系做访问学者,1999-2000 年在美国康乃尔大学动物学系博士后。长期从事本科生和研究生的生物化学,分子遗传学以及体细胞分子遗传学与细胞工程等课程的教学工作;科研上主要开展人及动物的细胞分子发育生物学相关领域的研究工作,如动物发育相关基因,性别决定与分化相关基因的分离克隆及作用机理的探索研究。发表SCI刊源及核心期刊研究论文四十多篇。获得过973项目、国家自然科学基金项目和教育部“新世纪优秀人才计划”资助。2002年获得四川省科技进步奖一等奖,2005年获得湖北省自然科学奖二等奖。
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6
【期刊论文】Characterization of Bovidae sex-determining gene SRY
程汉华, Hanhua CHENGa, *, Huifang SHIa, Rongjia ZHOUa, **, Yiqing GUOa, Li LIUa, Jiangdong LIUa, Yong JIANGa, Toshiyuki KUDOb, Shizuyo SUTOUb, ***
Genet. Sel. Evol. 33(2001)687-694,-0001,():
-1年11月30日
In mammals, testis determination is under the control of the sex-determining gene SRY. This Y-linked gene encodes a protein with a DNA binding domain similar to those found in high-mobility-group proteins. Here we report the cloning and sequences of the SRY genes of yak and Chinese native cattle. Our data show that SRY genes in Bovidae are less divergent, especially in the coding and 30 regions.
sex determination/, SRY/, evolution/, Bovidae
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程汉华, Hongshi Yu, Hanhua Cheng, Yiqing Guo, Laixin Xia, and Rongjia Zhou*
Biochemical and Biophysical Research Communications 307(2003)165-171,-0001,():
-1年11月30日
Several mechanisms were used in determination of the development of the male or female of vertebrates. The genes for determination of sequential hermaphrodite sex are unknown. Here, we reported cloning, alternative splicing, and expression patterns of the CYP17 gene of the rice field eel, a teleost fish with a characteristic of nature sex reversal. The CYP17 gene of the rice field eel was clustered into the CYP17 gene group of all the other vertebrates, especially into the fish subgroup. Four isoforms of the CYP17 were generated in gonads by alternative splicing and polyadenylation. Alternative splicing events of all these isoforms occurred in 30 regions, which encoded three different sizes (517, 512, and 159 aa) of proteins. RT-PCR results indicate specific expression in gonads of these isoforms. Northern blot analysis shows that expression patterns of the CYP17 (dominantly expressed in testis, less in ovary, and the least in ovotestis) are consistent with the sex reversal process of the rice field eel. In situ hybridization further shows its specific expression in germinal lamellae, the gonadal epithelium of the gonads. These findings indicate that CYP17 is differentially regulated in a sex-and developmentally specific manner, suggesting that the CYP17 potentially has important roles in gonad differentiation during sex reversal of the rice field eel.
Alternative splicing, Sex reversal, CYP17, The rice field eel
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【期刊论文】SRY-related genes in the genome of the rice feld eel (Monopterus albus)
程汉华, Rongjia ZHOUa, *, Hanhua CHENGa, Quiyang ZHANGb, Yiqing GUOa, Richard K. COOPERb, Terrence R. TIERSCHc
Genet. Sel. Evol. 34(2002)129-137,-0001,():
-1年11月30日
The mammalian sex determining gene, SRY, is the founding member of the new growing family of Sox (SRY-like HMG-box gene) genes. Sox genes encode transcription factors with diverse roles in development, and a few of them are involved in sex determination and differentiation. We report here the existence of Sox genes in the rice feld eel, Monopterus albus, and DNA sequence information of the HMG box region of ve Sox genes. The Sox1, Sox4 and Sox14 genes do not have introns in the HMG box region. The Sox9 gene and Sox17 gene, which each have an intron in the conserved region, show strong identity at the amino acid level with the corresponding genes of mammals and chickens. Similar structure and identity of the Sox9 and Sox17 genes among mammals, chickens and sh suggest that these genes have evolutionarily conserved roles, potentially including sex determination and differentiation.
sh/, Sox/, cloning/, sexdetermination
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【期刊论文】Molecular cloning, characterization, and expression in brain and gonad of Dmrt5 of zebrafish
程汉华, Yiqing Guo, Qin Li, Shang Gao, Xiang Zhou, Yan He, Xuan Shang, Hanhua Cheng*, Rongjia Zhou*
Biochemical and Biophysical Research Communications 324(2004)569-575,-0001,():
-1年11月30日
The DM domain gene family has at least eight members with conserved DNA-binding DM domain, which encodes putative transcription factors related to the sexual regulator Dsx of Drosophila and Mab-3 of Caenorhabditis elegans. Although some of the DM genes are involved in sexual development, the function of most of these genes remains unclear. There is also few structural and functional analysis concerning DM domain genes of the model fish, zebrafish. We report here molecular cloning, sequence, and expression of zebrafish Dmrt5, which consists of two exons, and encodes a 440-amino acid protein with conserved DMA and DMB domains in addition to DM domain. Phylogenetic analysis shows that zebrafish Dmrt5 fits within the Dmrt5 clade of fish and mammals. Zebrafish Dmrt5 was expressed in early gastrula period, subsequently increased to a high level in late stage of gastrula period (bud stage) and lower until the hatch period. In situ hybridization analysis showed its expression in developing central nervous system of embryos, especially in mid-brain and mid-hind brain boundary. In adult, its expression was restricted in brain and developing germ cells, especially in spermatogonia, spermatocytes, spermatids, and sperm cells, and in developing oocytes, including early perinucleolus stage oocyte, late yolk vesicle stage oocyte, and oil drop stage oocyte. These data suggest that zebrafish Dmrt5 have potentially important roles in gonadal development and may have contributed to the functional endocrine axis.
DM domain, Doublesex, Dmrt5, Sexual differentiation, Zebrafish
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【期刊论文】Gene structure, multiple alternative splicing, and expression in gonads of zebrafish Dmrt1
程汉华, Yiqing Guo, Hanhua Cheng *, Xiao Huang, Shang Gao, Hongshi Yu, Rongjia Zhou *
Biochemical and Biophysical Research Communications 330(2005)950-957,-0001,():
-1年11月30日
Many basic cellular processes are shared across vast phylogenetic distances, whereas sex-determining mechanisms are highly variable between phyla although the existence of two sexes is nearly universal in the animal kingdom. The only molecular similarity in sex determination found so far between phyla is among the fly doublesex, worm mab-3, and vertebrate Dmrt1/DMY, which contain a zinc-finger-like DNA-binding motif, DM domain. Here we report that three isoforms of the zebrafish Dmrt1 were generated in gonads by multiple alternative splicing, which encoded predicted proteins with 267, 246, and 132 amino acids, respectively. By cDNA cloning and genomic structure analysis, we found that there were seven exons of Dmrt1, which were alternatively spliced to generate the Dmrt1 isoforms. Northern blotting analysis revealed that expression of zebrafish Dmrt1 was higher in testis than ovary. Real time fluorescent quantitative RT-PCR indicated that expression of isoform a of Dmrt1 was dominantly higher than those of Dmrt1 b and c. Furthermore, in situ hybridization to gonads sections showed that Dmrt1 was expressed in developing germ cells of both testis and ovary, suggesting that the Dmrt1 gene is not only associated with testis development, but also, may be important in ovary differentiation of zebrafish.
Alternative splicing, Doublesex, Sex differentiation, Fish
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程汉华, Xiao Huang, Yiqing Guo, Yi Shui, Shang Gao, Hongshi Yu, Hanhua Cheng, and Rongjia Zhou
BIOLOGY OF REPRODUCTION 73, 1017-1024 (2005),-0001,():
-1年11月30日
Morphologically distinct males and females are observed throughout the animal kingdom. Why and how sex evolved and is maintained in most living organisms remains a key question in cellular and evolutionary biology. Here we report that four isoforms of dmrt1 (dsx-and mab3-related transcription factor 1) are generated in testis, ovotestis, and ovary by alternative splicing in the rice field eel, a fresh water fish that undergoes natural sex reversal from female to male during its life cycle. These transcripts encode four different size proteins with 301, 196, 300, and 205 amino acids. Like fly doublesex splicing, the dmrt1 of the rice field eel is also alternatively spliced at the 39 region, which generates diverse isoforms in gonads by alternative use of 39 sequences. Not only is dmrt1 expressed specifically in gonads, but its multiple isoforms are differentially coexpressed in gonadal epithelium during gonad transformation. Expression levels of a and b isoforms of dmrt1 ranged from low to high (ovary,ovotestis I, ovotestis II, ovotestis III,testis), based on comparisons of mean values from real-time fluorescent quantitative reverse transcription-polymerase chain reaction analysis. The overall expression level of dmrt1 b was much lower than that of dmrt1 a. Expression of dmrt1 d was not only low, but it also did not change significantly during sex transformation. The differential expression of dmrt1 isoforms may also be regulated by their 39 untranslated regions (UTRs), although these 39 UTRs do not contribute to intracellular localization of the Dmrt1 protein. These results provide new insight into roles of regulation at the level of splicing of dmrt1 in governing the sex differentiation cascade.
alternative splicing,, developmental biology,, dsx,, fish,, ovary,, sexual development, testis
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