李德全
长期从事植物抗逆生理及其分子机制研究,研究领域包括:植物抗逆信号转导;植物在干旱逆境下的渗透调节作用;活性氧伤害及其清除机制;逆境蛋白的生理功能;光合作用光抑制及光破坏防御机制;根信号对植物水分利用效率的调节和作物根系适应干旱逆境生理。
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- 姓名:李德全
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学术头衔:
博士生导师
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学科领域:
植物学
- 研究兴趣:长期从事植物抗逆生理及其分子机制研究,研究领域包括:植物抗逆信号转导;植物在干旱逆境下的渗透调节作用;活性氧伤害及其清除机制;逆境蛋白的生理功能;光合作用光抑制及光破坏防御机制;根信号对植物水分利用效率的调节和作物根系适应干旱逆境生理。
李德全,男,1955年生,山东博兴县人,教授,博士生导师。现任山东农业大学生命科学学院教授委员会主任,兼任山东省植物生理学会理事长,中国植物生理学会常务理事兼教科工作委员会主任委员。1978年毕业于山东农学院(现山东农业大学),留校从事植物生理学教学和科研工作至今。1985-1988年在职读植物生理生化研究生,获硕士学位。1991年被山东省教委确定为省属高校首批中青年学术骨干学科带头人人选,1994年晋升为教授,1996年被山东省学位委员会遴选批准为博士生导师,1997年获国务院政府特殊津贴。长期从事植物抗逆生理及其分子机制研究,研究领域包括:植物抗逆信号转导;植物在干旱逆境下的渗透调节作用;活性氧伤害及其清除机制;逆境蛋白的生理功能;光合作用光抑制及光破坏防御机制;根信号对植物水分利用效率的调节和作物根系适应干旱逆境生理。
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【期刊论文】Expression of MaMAPK Gene in Seedlings of Malus L. under Water Stress
李德全, Peng Lixinl, Gu Lingkun, Zheng Chengchao, Li Dequan, Shu Huairui
,-0001,():
-1年11月30日
Three apple materials: Malus hupehensis, drought-sensitive, M. sieversii, a drought-tolerance type, and M. micromalus, a middle type, were used in this experiment. The expression characteristics of MaMAPK gene in the three materials were analyzed by Northern blotting. Results showed that MaMAPK gene was expressed in roots and leaves (except for control) of the three species seedlings treated with 20% PEG for different time, and its expression gradually increased with the time, whose peak emerged when the seedlings were treated for 1.5hr, then its expression decreased gradually. Liquid kinase assays indicated that the dynamic changing trend of MAPK kinase activity was very similar to that of the mRNA relative expression amount of MaMAPK. The differences between were that the peak of kinase activity emerged later than the latter's. Although the increase amount of kinase activity decreased after the peak, it was still higher than that of the control until the end of treatment. These results suggested that MaMAPK gene was regulated not only by water stress on transcription level, but also by phosphorylation and dephosphorylation on protein level. In the three researched apple species, the dynamic change of kinase activity was similar to that of the mRNA relative expression amount of MaMAPK. However, there were the highest MAPK activity and the highest mRNA relative expression amount of MaMAPK in M. sieversii, followed by M micromalus and M. hupehensis. The results indicated that the mRNA relative expression amount of MaMAPK and the activity of MAPK both correlated with the ability of drought-tolerance in the three species. Therefore, the differences between the mRNA relative expression amount of MaMAPK and the activity of MAPK were one molecular mechanisms of the different drought-tolerance in the three species of Malus L..
Water stress, Malus L., , MaMAPK gene, Expression characteristics
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李德全, Dapeng Li, , Yunfeng Xu, Gangming XU, Lingkun GU, Dequan Li, Huairui Shu
D. Li et al. Phytochemistry 67 (2006) 658-667,-0001,():
-1年11月30日
Volatile esters are major aroma components of apple, and an alcohol acyltransferase (AAT) catalyzes the final step in ester biosynthesis. The gene MdAAT2, which encodes a predicted 51.2kDa protein containing features of other acyl transferases, was isolated from Malus domestica Borkh. (cv. Golden Delicious). In contrast to other apple varieties, the MdAAT2 gene of Golden Delicious is exclusively expressed in the fruit. The MdAAT2 protein is about 47.9kDa and mainly localized in the fruit peel, as indicated by immunoblot and immunolocalization analysis. Northern blot and immunoblot analysis showed that the transcription and translation of MdAA T2 have a positive correlation with apple AAT enzyme activity and ester production, except in the later ripening stage, suggesting that MdAAT2 is involved in the regulation of ester biosysthesis and that a post-translation modification may be involved in regulation of AAT enzyme activity. Tissue disk assays of fruit peel revealed that using extraneous alcohols can recover the corresponding ester formation. Transcription and translation of MdAAT2 were both depressed by 1-methylcyclopropene (1-MCP) treatment and subsequent ester production was also prevented. These results suggest that: (1) ester production is mainly regulated by MdAAT2; (2) ethylene is also involved in this regulatory progress and (3) ester compounds rely principally on the availability of substrates.
Malus domestica Borkh., , Rosaceae, Apple, Gene expression, Development, Ripening, Alcohol acyltransferase, MdAAT2, Esters
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李德全, DA-PENG LI, YUN-FENG XU, LI-PING SUN, LI-XIA LIU, XIAO-LI HU, DE-QUAN LI, HUAI-RUI SHU
J. Agric. Food Chem. 2006, 54, 3887-3895,-0001,():
-1年11月30日
Volatile esters, primarily synthesized in peel tissues, are major aromatic components of apple fruits [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.]. The use of cold storage combined with 1-methylcyclopropene (1-MCP) treatment prolongs the life of apples but represses the regeneration of esters during poststorage ripening. In this study, the regeneration of total esters was significantly increased in apple fruits treated with salicylic acid (SA) and Ethephon (ETH) that had been treated once or twice with 1-MCP. However, methyl jasmonate (MeJA) treatment resulted in regeneration of total esters after a single 1-MCP treatment. To determine the mechanism by which SA, ETH, and MeJA regulate ester regeneration, the apple alcohol acyltransferase gene (MdAAT2) was investigated at the mRNA, protein, and enzyme activity levels. Genes associated with ethylene perception were also investigated by RT-PCR. The results suggest that MdAAT2 controls ester regeneration and that MdETR1 plays a key role in ethylene perception and regulation of downstream MdAAT2 gene expression during poststorage. Ester compounds and concentrations differed in peels treated with different signal molecules, indicating that regulation of the pathway upstream of straight-chain ester biosynthesis depended on the regulation of lipoxygenase (LOX) and alcohol dehydrogenase (ADH) activity by SA, ETH, and MeJA during poststorage ripening.
Alcohol acyltransferase, apple [Malus sylvestris (, L., ), Mill., var., domestica (, Borkh., ), Mansf., ], ester regeneration, ethylene, poststorage ripening
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李德全, Qian-Quan Ma, , Wei Wang, Yong-Hua Li, De-Quan Li, Qi Zou
Journal of Plan: Physiology 163 (2006) 165-175,-0001,():
-1年11月30日
Effects of foltar application of 100 mmol/L glycinebetaine (GB) on PS II photochemistry in wheat (Triticum aestivum) flag leaves under drought stress combined with high irradiantce were investingated. The results show that GB-treated plants maintained a higher net photosynthetic rate during drought stress than non-GB treated plants. Exogenous GB can preserve the photochemical activity of PSII, for GB-treated plants maintain higher maximal photochemistry efficiency of PSII (Fv/Fm)and recover more rapidly from photoinhibition. In addition, GB-treated plants can maintain higher anti, oxidative enzyme activities and suffer less oxidative stress. Our data suggest that GB may protect the PSII complex from damage through accelerating D1 protein turnover and maintaining anti-oxidative enzyme activities at higher level to alleviate photodamage. Dieviyldithocarbamale as well as streptomycin treatment can impair the protective effect of GB on PSII. In summary, GB can enhance the photoinhibition tolerance of PSII.
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李德全, X.Q. GUAN, , S.J. ZHAO, D.Q. Li, H.R. SHU
PHOTOSYNTHETICA 42 (1): 31-36, 2004,-0001,():
-1年11月30日
Four grapevine cultivars, i.e. Cabernet Sauvignon (a member of the Western Europe cultivar group), Rizamat (a member of the East cultivar group), Red Double Taste (a hybridized cultivar from Vitis vinifera L. and V. labrusca L.), and 1103Paulsen (a hybridized rootstock), were treated by three severity orders of drought stress for 25 d. Then net photosynthetic rate (PN), maximal photochemical efficiency (Fv/Fm), actual photochemical efficiency (ΦPS2) of photosystem 2, total electron transport rate (JT), electron transport flows used in carboxylation (JC) and in oxygenation (Jo) reactions catalysed by ribulose-1, 5-bisphosphate carboxylase/ oxygenase were determined. PN was determined again after re-watering for 2 d by combined measurement of gas exchange and chlorophyll fluorescence. Along with the increase in severity of drought stress, PN, Fv/Fm, ΦPS2, JT, and Jc in all four cultivars decreased. The range of decrease differed among cultivars. Jo expressed various trends from cultivar to cultivar. In Rizamat that received slight and moderate drought stress, PN evidently decreased, but Jo markedly increased, thus maintaining high values of JT andΦPS2. Prior to the moderate drought stress, the FV/Fm was high in Rizamat, indicating that the photodamage had not happened ahead of the moderate drought stress given. Under the severe drought stress, the photorespiration rate in Rizamat decreased by 70 %, and JT, ΦPS2, and Fv/Fm also dropped to very low values, i.e. the photodamage of photosynthetic apparatus has taken place. This suggested that the photorespiration has consumed the excessive assimilatory power and the photo-protective function of photorespiration is very important for Rizamat. When Cabernet Sauvignon grew under drought stress, its Jo decreased in a small range, thus maintaining higher values of Jc, JT, ΦPS2 and Fv/Fm; hence no serious photodamage occurred. Despite of the fact that PN of cv. Red Double Taste decreased markedly under the slight drought stress, Jo still increased under the severe drought stress. This suggests that photorespiration is important in photoprotection under drought stress. Jo in cv. 1103Paulsen markedly decreased under slight stress. Accordingly, PN, Fv/Fm, ΦPS2, JT, and Jc decreased to extremely low values. Thus photorespiration effectively protects the photosynthetic apparatus from photo-damage under drought, assists in maintaining a relatively highΦPS2, and helps PN to be rapidly recovered after re-watering.
chlorophyll fluorescence, gas exchange, net assimilation rate, photoinhibition, Vitis vinifera L.,
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李德全, H.S. RA, , Y.Q. HAW, D.Q. LI
PHOTOSYNTHETICA 41 (1): 151-156, 2003,-0001,():
-1年11月30日
In the course of dehydration, the gas exchange and chlorophyll (Chl) fluorescence were measured under irradiance of 800
chlorophyll fluorescence, gas exchange, irradiance, Malus pumila, Mehler reaction, photorespiration, photosystem 2 non-cyclic electron transport.,
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【期刊论文】Responses of ABA and CTK to soil drought in leafless and leafy apple tree
李德全, LI Yan, PAN Hai-chun, LI De-quan
Journal of Zhejiang University SCIENCE V. 4, No. 1, P. 101-108, Jan. -Feb., 2003,-0001,():
-1年11月30日
The authors tested the contents of ABA (abscisic acid), ZR (zeatin riboside), DHZR (dihydrozeatin riboside) and iPA (isopentenyl adenosine) in leafless and leafy apple trees (Red Fuji/Males micromalus Makino) during soil drought stress. ABA concentration in drought stressed leafless trees increased significantly compared to the controls. ABA both in roots and xylem rose steadily in the earlier drought stage, reaching a maximum of 1.46
Apple tree, Endogenous hormone, Soil drought, Relative water content, Water potential
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李德全, JIA Hu-Sen, , LI De-Quan
植物学报Acta Botanica Sinica 2003, 45 (12): 1428-1433,-0001,():
-1年11月30日
The response of steady-state fluorescence (Fs) to irradiance in apple (Malus pumila Mill. cv. Tengmu No.1 /Malus hupehensis Rehd.) leaf increased and decreased at light levels below and above 400 μmol·m-2•s-1 photosynthetic photon flux density (PPFD), respectively, while the light-adapted maximal fluorescence (Fm') and minimal fluorescence (Fo') decreased constantly with the increasing PPFD, and the closure of photosystem II reaction center (PS II RC) increased continuously, reflected by the chlorophyll fluorescence parameter of (Fs -Fo')/ (Fm'-Fo'). These facts indicated that decrease of Fs above 400 µmol•m-2•s-1 PPFD was not caused by closure of PS II RC, but was mainly resulted from the process of light transfer from light-harvesting complex II (LHC II) to PS II RC. In the presence of Nethylmaleimide (NEM), an inhibitor of photosynthetic state transition, Fs kept on increasing in apple leaf at light levels from 400 to 700 µmol•m-2•s-1, which was the photosynthetic saturation irradiance of apple leaves. In addition, Fs still increased at light levels over 700 µmol•m-2•s-1 in apple leaf pre-treated with dithiothreitol (DTT), an inhibitor of xanthophyll cycle. These changes showed that state transition and xanthophyll cycle caused a decrease of Fs in apple leaf at light levels below and above the photosynthetic saturation irradiance, respectively. When apple leaf was pre-treated with NEM, the PS II apparent rate of photochemical reaction (P-rate) and photochemical quenching (qP) decreased significantly in the light range of 600-800 µmol•m-2•s-1, but the non-photochemical quenching (qN) existed a small increase at 600-800 µmol•m-2•s-1 and a decrease above 800 µmol•m-2•s-1. These phenomena suggested that state transition was mainly a photochemical and a non-photochemical process in apple leaf responding to light lower and higher than photosynthetic saturation irradiance, respectively.
state transition, chlorophyll fluorescence, light response
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【期刊论文】土壤干旱下不同抗旱性小麦品种的渗透调节和渗透调节物质
李德全, 邹琦, 程炳嵩
植物生理学报/Acta Phytophysiologica Sinica 1992, 18(1): 37-44,-0001,():
-1年11月30日
在土壤干旱下抗旱性强的小麦品种的渗透调节能力大于抗旱性弱的品种。土壤缓慢干旱时,几种主要渗透调节物质增加的先后次序为:Pro,K+,然后是可溶性糖和其他游离氮基酸,后两者几乎平行增加、在土壤干旱下的渗透调节物质中,有机溶质为可溶性糖,其他游离氢基酸,Pro和Mal;无机离子为K+,Ca2+,Mg2+等。对渗透调节的相对贡献率为:K+>可溶性糖>其他游离氧基酸>Ca2+>Mg2+>Pro。六种物质总和对渗透调节的相对贡献率为;轻度干旱56%—70%.中度干旱70%--79%,严重干旱79%--88%。
小麦, 土壤干旱, 渗透调节, 渗透调节物质
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【期刊论文】土壤水分胁迫对小麦叶片的渗透调节与延伸生长的影响
李德全, 邹琦, 程炳嵩
植物学报Acta Botanica Sinica 1992, 34 (2): 121-125,-0001,():
-1年11月30日
两个小麦品种叶片延伸速率随着土壤水分胁迫的加剧而下降,复水后“昌乐5号”恢复生长比“鲁麦5号”快.随着水势的下降,两个品种叶片渗透调节能力增加,“昌乐5号”约为0.4IMPa;“鲁麦5号”约为0.33MPa。在同一叶片延伸速率下,“昌乐5号”的水势、渗透势总是比“鲁麦5号”的更低,生长停止时前者的水势、渗透势分别为一1.50MPa、-l.70MPa左右;而后者剧分别为一1.20MPa和-1.30MPa左右。叶片细胞延伸生长的临界膨压不同,“昌乐5号”为0.22MPa,“鲁麦5号”为0.15MPa,扩张系数变化很小。
小麦, 土壤水分胁迫, 渗透调节, 叶片延伸速率
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