王进军
昆虫分子生态学、昆虫分子毒理学、储藏物昆虫学、入侵生物学、有害生物综合治理等
个性化签名
- 姓名:王进军
- 目前身份:
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学术头衔:
博士生导师, “973”、“863”首席科学家, 优秀教师/优秀教育工作者
- 职称:-
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学科领域:
肿瘤学
- 研究兴趣:昆虫分子生态学、昆虫分子毒理学、储藏物昆虫学、入侵生物学、有害生物综合治理等
王进军,男,1970年生,1997年毕业于西南农业大学并获理学博士学位。现任西南农业大学教授、博士研究生导师,植物保护学院院长、昆虫学及害虫控制工程重庆市重点实验室主任、国际Systematic and Applied Acarology刊物、国内《中国南方果树》编委、中国粮油学会常务工作委员及储藏分会常务理事兼害虫防治学科组组长、重庆市植物保护学会副理事长。先后在英国伦敦大学皇家学院和美国佛罗里达大学大学深造,并多次赴泰国和日本等国进行访问或合作研究。主要从事昆虫分子生态学、昆虫分子毒理学、储藏物昆虫学、入侵生物学、有害生物综合治理等领域的研究与教学工作,特别是在昆虫适应环境胁迫的分子生态学研究方面具有较深的造诣。先后主持国家自然科学基金项目、国家重点基础研究(973)项目子专题、霍英东优秀青年教师科研基金、教育部科学技术研究重点项目、教育部博士点基金、教育部留学回国人员科研启动基金、人事部留学回国人员择优资助项目、国家外国专家局农业引智成果示范推广项目、市科委科技攻关等项目等20余项。在国际上首次报道了储藏物害虫书虱对气调处理产生抗性的基因潜力,并在实验室选育出了数个抗性品系,阐明了抗性发生发展的生理生化机理。应用比较毒理学的研究方法,系统研究了几种常见书虱对不同类型杀虫剂的敏感性差异,并就差异的生化机理进行了探讨,克隆了嗜卷书虱乙酰胆碱酯酶的基因片段。研究了书虱体内共生微生物Wolbachia对书虱的生殖调控作用,有望开辟出储藏物害虫生物防治的新途径。系统研究了朱砂叶螨适应酸雨胁迫的分子生态学机理,并就不同品系的适合度进行了评估,研究结果对于酸雨污染区害螨综合治理具有极大的参考价值。初步研究了生态系统对紫茎泽兰入侵的抵御机制。发表论著90余篇,18篇为SCI收录,获省部级科技进步一等奖1项,2001和2002年先后获中国植物保护学会首届、中国昆虫学会第三届青年科技奖,2002年入选重庆市首批优秀青年骨干教师培养计划并于2004年获重庆市首批优秀青年骨干教师称号。2004年评为全国优秀教师,同年入选“教育部新世纪优秀青年教师支持计划”。
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王进军, JIN-JUN WANG, , JAMES H. TSAI, WEI DING, ZHI-MO ZHAO, AND LUNG-SHU LI
J. Econ. Entomol. 94 (5): 1296-1301 (2001),-0001,():
-1年11月30日
Six plant essential oils alone as repellent and fumigant, and in combination with the controlled atmosphere against Liposcelis bostrychophila Badonnel were assessed in the laboratory. These essential oils were extracted from the leaves of six source plants: Citrus tangerina Tanaka, Citrus aurantium L., Citrus bergamia Risso et Poiteau, Pinus sylvestris L., Cupressus funebris Endl., and Eucalyptus citriodora Hook. The repellency test indicated that L. bostrychophila adults were repelled by FIlter paper strips treated with six essential oils. Of these essential oils, the C. funebris oil was most effective followed by that of P. sylvestris, C. tangerina, C. bergamia, and E. citriodora. The average repellency of the C. aurantium oil against L. bostrychophila adults was significantly lower than other five test oils by day 14. These essential oils had a high level of toxicity in the fumigation assay against L. bostrychophila adults at both 10 and 20ppm. When combined with two controlled atmosphere treatments (12% CO2+9% O2, and 10% CO2+5% O2, balanced N2), the toxicity of plant oils was enhanced significantly.
Liposcelis bostrychophila,, plant essential oil,, controlled atmosphere,, toxicity
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王进军, J.-J. Wang, Z.-M. Zhao and J.-P. Zhang
,-0001,():
-1年11月30日
The host plant-mediated impact of simulated acid rain (pH 3.0, 3.5, 4.0, 5.6, and 6.8) on the behaviour, development, and reproduction of carmine spider mite, Tetranychus cinnabarinus (Boisduvals), were evaluated at 25℃ in a series of laboratory trials. The results indicated that the adults of carmine spider mite prefer to aggregate on pH 4.0 acid rain-treated eggplant leaves. The developmental times of nymph, larva and the pre-oviposition period of the mite on kidney bean leaves were significantly affected by acid rain treatments. The developmental times for the immature stages ranged from 11.4 days on pH 4.0 acid rain-treated leaves to 12.7 days on other acid rain-treated leaves. The survival of immatures varied from 85.14% on pH 4.0 acid rain-treated leaves to 78.83% on deionized water-treated leaves (pH 6.8). The mites feeding on acid rain-treated leaves (pH 3.0-5.6) had significantly greater reproductivity and longevity than those feeding on deionized water-treated leaves. The average greatest reproductivity (74.6 eggs per female) and longest female longevity (8.9 days) were recorded on pH 4.0 acid rain-treated leaves. Accordingly, the intrinsic rate of increase (rm) for the mite on pH 4.0 acid rain-treated leaves was the highest. Jackknife estimates of rm varied from 0.201 on pH 4.0 acid rain-treated leaves to 0.158 on pH 5.6 acid rain-treated leaves. The results implied that the population growth of the mite were enhanced by application of acid rain (pH 3.0-5.6) on host plants.
acid rain,, carmine spider mite,, host plant-mediated impact,, life table parameters,, preference
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王进军, Jin-Jun Wang, Wei-Xia Cheng, Wei Ding, Zhi-Mo Zhao
,-0001,():
-1年11月30日
The inhibition kinetics of dichlorvos on carboxylesterase and acetylcholinesterase (AChE) activity extracted from Liposcelis bostrychophila and L. entomophila (Psocoptera: Liposcelididae) were compared. The results showed that L. entomophila had significantly greater specific activity of carboxylesterase than L. bostrychophila (0.045 versus 0.012μmoles/mg/min). Moreover, the carboxylexterase of L. entomophila showed higher affinity (i.e. lower Km value) to the substrate 1-naphthyl acetate than L. bostrychophila (0.29 versus 0.67mM). The specific activity and affinity of AChE of the two species were not significantly different. The carboxylesterase of L. bostrychophila was more sensitive to the insecticide dichlorvos than that of L. entomophila. The I50s values of dichlorvos to carboxylesterase for L. bostrychophila and L. entomophila were 1.43 and 3.28μM, respectively, and to AChE were 324 and 612nM, respectively. Inhibition kinetics revealed that AChE from L. bostrychophila was 5.8-fold more sensitive to inhibition than AChE from L. entomophila.
psocid, susceptibility, carboxylesterase,, AChE,, DDVP
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王进军, JAMES H. TSAI, JIN-JUN WANG AND YING-HONG LIU
Florida Entomologist 85 (3) September 2002,-0001,():
-1年11月30日
Seasonal abundance of the Asian citrus psyllid, Diaphorina citri Kuwayama, was studied weekly in two orange jasmine [Murraya paniculata (L.) Jack] plots in southern Florida from October 1998 to October 1999. Psyllid populations occur throughout the season on orange jasmine in southern Florida. Population peaks were observed in October, November, and December in 1998, and May and August in 1999. Psyllid population levels were positively related to the availability of new shoot flushes which were in turn related to the weekly minimum temperature and rainfall. Natural enemies were not key factors in regulating populations during the study period. The populations of adult psyllids were also studied weekly on potted orange jasmine and grapefruit (Citrus paradise Macfadyen) plants from June 1999 to July 2000. The population levels of psyllid on both host plants were not significantly different and general population trends on the two hosts were similar over time. Continuous shoot flushes produced by orange jasmine could play an important role in maintaining high populations of this insect when new shoot flushes were not available in the commercial citrus groves.
Asian citrus psyllid,, seasonal abundance,, temperature,, rainfall
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【期刊论文】SAMPLING OF DIAPHORINA CITRI (HOMOPTERA: PSYLLIDAE) ON ORANGE JESSAMINE IN SOUTHERN FLORIDA
王进军, JAMES H. TSAI, JIN-JUN WANG, AND YING-HONG LIU,
Florida Entomologist 83 (4) December, 2000,-0001,():
-1年11月30日
Dispersion indices and related statistics of Asian citrus psyllid, Diaphorina citri Kuwayama, on orange jessamine [Murraya paniculata (L.) Jack] shoots in southern Florida from 1998 to 1999 were determined with 235 data sets and used to develop sampling plans. Three regression models, Taylor's power law, Iwao's patchiness regression, and k=c+dm [k=m2/(S2-m)] (where k is the parameter for the negative binomial distribution) were used to analyze the data. Taylor's power law (a= 0.3407
Asian citrus psyllid,, Taylor', s power Law,, Iwao', s patchiness regression,, negative bionomial distribution
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王进军, Jin-Jun Wang, Zhi-Mo Zhao, James H. Tsai*
Journal of Stored Products Research 36(2000)297-308,-0001,():
-1年11月30日
Two populations (S-1 and S-2) of the psocid, Liposcelis bostrychophila Badonnel were exposed to carbon dioxide enriched atmospheres. Carbon dioxide resistance developed at steady rates in these two populations during this study period. Selection with 35 and 55% CO2 resulted in resistance development as expressed by LT50. Resistance increased steadily under continuous selection to 4.6- and 5.3-fold by generation F30 for S-1 and S-2, respectively. Throughout the selection process, the slopes of regression lines were always lower than that of the control. The results of biochemical assays showed that the activities of carboxyl esterase (CarE) and superoxide dismutase (SOD) in vitro increased in the selection process. Exposure to higher CO2 content (HCC) resulted in a gradual decrease in CarE activity in both selected and control populations. Although the induction effect of CO2 on SOD was brief, the induction times for the S-1 and S-2 were greater than those of the control. The elevated catalase (CAT) activity in association with resistance development was also evident, but no statistical correlation was found between CAT activity and HCC resistance. No significant differences were found in acid phosphatase and alkaline phosphatase activities in both selected and control populations during this study. This study demonstrated that high CarE and SOD activities were positively correlated to CO2 resistance.
Liposcelis bostrychophila, Resistance, Controlled atmosphere, Enzyme activity
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王进军, JIN-JUN WANG†‡, JAMES H. TSAI*†, ZHI-MO ZHAO‡ and LUNG-SHU LI‡
INTERNATIONAL JOURNAL OF PEST MANAGEMENT, 2001, 47(1)55-62,-0001,():
-1年11月30日
The interactive effects on the development, survivorship and reproduction of Liposcelis bostrychophila Badonnel, of two biologically relevant controlled atmosphere (CA) environments 10% CO2+5% O2, and 12%CO2+9% O2, were determined at eight constant temperatures (17.5, 20, 22.5, 25, 27.5, 30, 32.5 and 35℃) in the laboratory. The life cycle parameters measured under nonmodified atmosphere conditions served as a control. A population reared at 17.5℃ failed either to develop or to reproduce regardless of atmospheric conditions, whereas populations reared under combined conditions successfully completed their life cycles. However, development, survivorship, and reproduction were all inhibited by hypoxia and hypercarbia compared with the control atmosphere. Egg development was more sensitive to 12% CO2 than to 10% CO2 exposure. Nymphal development, survivorship, and reproduction were more sensitive to 5% O2 (10% CO2) than 9% O2 (12% CO2). Sensitivity was evident at higher temperatures. The first and second nymphal instars were the most sensitive life stages to CA exposure. We conclude that 10±12% CO2 and 5±9% O2 atmospheres found in sealed storage containers as the result of respiration of the grain, insect and related organisms, would not be effective in the control of L. bostrychophila.
Liposcelis bostrychophila,, development,, reproduction,, temperature,, controlled atmosphere
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王进军, W.-X. Cheng, J.-J. Wang, W. Ding and Z.-M. Zhao
,-0001,():
-1年11月30日
The inhibition kinetics on carboxylesterase (CarE) andacetylcholinesterase (AChE) of Liposcelis bostrychophila and Liposcelis entomophila of paraoxon andcarbosulfan were compared. The results showedthat L. entomophila exhibitedsignificantly greater specific activity of CarE than L. bostrychophila [0.045vs. 0.012lmol of a-naphthyl acetate (a-NA) hydrolysed/mg protein/min]. Moreover, L. entomophila showedhigher affinity (i.e. lower Km value) to the substrate a-NA than L. bostrychophila (0.29vs. 0.67mm). For AChE-specific activity andaffinity, no significant differences between the two species were observed. Based on the I50 values, AChE of L. bostrychophila was more sensitive to paraoxon andcarbosulfan than that of L. entomophila. According to inhibition kinetics, the results revealedthat AChE of L. bostrychophila was 3.8-foldmore sensitive to inhibition by paraoxon than that of L. entomophila, but L. entomophila was 1.5-foldmore susceptible to carbosulfan.
carbamates,, esterase,, organophosphates,, psocid,, susceptibility
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王进军, JINJUN WANG, ZHIMO ZHAO and LUNGSHU LI
INTERNATIONAL JOURNAL OF PEST MANAGEMENT, 1999, 45(1)75-79,-0001,():
-1年11月30日
Adult populations of the psocid, Liposcelis bostrychophila, were exposed for 30 generations to 35% CO2, 1% O2, and 64% N2 at 28℃, 80% RH, in order to select a strain resistant to controlled atmosphere (CA). Selection pressure was maintained at around 70% mortality. At the 30th generation, comparison of sensitivity between the selected strain and the original susceptible strain (CA-S) indicated a tolerance factor (TF) at the 50% mortality level (LT50) of 5.6-fold. Throughout the selection process, log-time against probit-mortality lines remained roughly parallel and the slopes remained lower than that of CA-S until the last generation. Implications are that at high levels of selection, multiple genetic factors continued to act together even at the 30th generation to select for adaptation to survival in a CA environment. Removal of selection pressure for five generations from one subpopulation of the selected strains from the 25th generation caused significant reduction in resistance. In the absence of CA exposure, the selected strain possessed the fitness defect. The CA-selected strain was calculated by R0 to have a fitness value of 0.39 relative to CA-S. In addition, the potential effective measures in the slowdown of resistance to CA of stored-product insects are also discussed.
Liposcelis bostrychophila,, controlled atmosphere,, resistance,, stability,, fitness
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王进军, James H. TSAI* and Jin-Jun WANG
BioControl 47: 645-655, 2002.,-0001,():
-1年11月30日
The effects of host age and parasitism by Lysiphlebia mirzai Shuja-Uddin (Hymenoptera: Braconidae) on the development, survivorship, reproduction, and life table parameters of brown citrus aphid, Toxoptera citricida (Kirkaldy) (Homoptera: Aphididae) were evaluated at 25
biological control,, host age preference,, Lysiphlebia mirzai,, population growth,, Toxoptera citricida
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