李永生
分析化学;仪器分析;工业分析;流动注射分析(FIA);激光粒度分布分析;高精度粘度分析;奥格电子探针分光光度分析(Auger); X射线分析;扫描电镜(SEM or FE-SEM);工业水处理;火电厂及核电站水化学;计算机的应用;水净化系统的设计等
个性化签名
- 姓名:李永生
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
应用化学(具体应用入有关学科)
- 研究兴趣:分析化学;仪器分析;工业分析;流动注射分析(FIA);激光粒度分布分析;高精度粘度分析;奥格电子探针分光光度分析(Auger); X射线分析;扫描电镜(SEM or FE-SEM);工业水处理;火电厂及核电站水化学;计算机的应用;水净化系统的设计等
李永生,在日本留学、工作近十年;90年到日本留学,在日本获得理学博士学位、完成博士后研究;兼任美国Wiley出版社《Laboratory Robotics and Automation》英文期刊的海外编辑;从事专业是应用化学和分析化学;此外,曾担任过日本电力中央研究所研究员、日本法政大学化学讲师、日本立教(圣保罗)大学客座研究员等职;1999年从日本电力中央研究所辞职归国,任东北电力学院应用化学系主任(正处级),2004年作为引进人才调入四川大学化学工程学院(获川大启动资金50万元)。现任四川大学化学工程学院副院长,教授,博导;中国电机工程学会电厂化学专业委员会委员。
主要学科:分析化学;仪器分析;工业分析;流动注射分析(FIA);激光粒度分布分析;高精度粘度分析;奥格电子探针分光光度分析(Auger); X射线分析;扫描电镜(SEM or FE-SEM);工业水处理;火电厂及核电站水化学;计算机的应用;水净化系统的设计等。
主要研究内容:快速自动化学分析方法、仪器及装置的开发和研制;工业在线自动分析方法、仪器及装置的开发和研制;环境监测自动分析方法、仪器及装置的开发和研制;临床自动分析方法、仪器及装置的开发和研制;流动注射分析(FIA)理论、方法及仪器装置的研究和开发;带循环流动注射分析(ZCFIA)理论、方法及仪器装置的研究和开发;火电厂及核电站金属材料的腐蚀研究;火电厂及核电站水处理研究;计算机同时处理中、英、日文的应用研究。
1987年,撰写了《流动注射分析》专著(第一作者),已由北京大学出版社和中国光学会联合出版发行;2002年撰写英文版专著一部:《Flow Injection Analysis and Application for Chemistry Analysis》(吉林人民出版社,2002)。1985 研制成功FIA-T1-721型FIA分光光度分析仪,获电力部科技进步奖、国家级重大科技成果。分别在《Anal. Chim. Acta》(荷兰,英文), 《Anal. Science》(日本,英文), 《The Thermal and Nuclear Power》(日文),《Laboratory Robotics and Automation》(美国,英文), 《ぶせきかがく》(日文),《分析化学》(重要期刊),《电机工程学报》(重要),《中国电力》(重要),《分析实验室》(核心),《理化检验》(核心),《工业水处理》(核心),《仪表技术与传感器》(核心)等国内外重要期刊杂志上发表论文65篇,其中,英文论文13篇、日文论文11篇、中文论文41篇,被SCI收录的论文17篇,被EI收录的论文14篇。
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成果阅读
226
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成果数
5
李永生, Xiu-Feng Gaoa, ∗, Yong-Sheng Li b, Isao Karube c
Analytica Chimica Acta 443(2001)257-264,-0001,():
-1年11月30日
A simple assay of sulfated bile acid (SBA) in urine using flow injection (FI)-spectrophotometry with immobilized enzyme reactors is proposed. The system consists of an injection valve, a switch valve, two immobilized enzyme reactors and a UV-VIS detector with a flow cell. The multi-step enzymatic reactions will occur when an injected sample containing SBA passes through the immobilized enzyme reactors. First, SBA will desulfate under catalysis of immobilized bile acid sulphate sulfatase (BSS), to form 3β-hydroxyl bile acids; the produced 3-hydroxyl bile acid reacts with nicotinamide adenine dinucleotide (NAD+) under catalysis of co-immobilized 3β-hydroxylsteroid dehydrogenase (3β-HSD), and is converted to the 3-ketosteroid. Meanwhile, β-NAD+ is converted to reduced nicotinamide adenine dinucleotide (NADH). Then by catalysis of immobilized diaphorase, NADH reacts with a novel reagent called "water soluble tetrazolium blue-5" (WST-5) to generate a blue diformazan dye, which is detected at 550 nm. By using FI-spectrophotometry manifold and optimized conditions, we have obtained a linear response for 1-75βM glycolithocholate sulfate (GLCA-S) with a correlation coefficient of 0.999 and an analytical rate of 15 samples per hour. The R.S.D. was less than 1%. The recoveries (91-108%) of GLCA-S added into urine were satisfactory and the assay correlated well with the manualUBASTECmethod. Therefore, it will be applicable for urine tests on patients suffering from hepatobiliary disease.
Sulfated bile acid, Bile acid sulphate sulfatase, Water soluble tetrazolium blue-5, Flow injection analysis, Spectrophotometry, Immobilized enzymes
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李永生, Yong-Sheng Li∗, Yu Muo, Hou-Mei Xie
Analytica Chimica Acta 455(2002)315-325,-0001,():
-1年11月30日
A new flow injection spectrophotometric method is described for the simultaneous determination of silicate and phosphate. Effects on the sensitivity of the method of the wavelength, temperature, length of reaction coils, pump rates, acidity, sampling volume, concentration of the chromogenic reagent, etc. were also investigated. The optimum conditions were ascertained. The principle of the method is that total concentration of silicate plus phosphate is determined when a injected sample plug is passing through the first flow cell and then the concentration of silicate is serially) determined at a second flow cell of the same detector after continuously masking the yellow molybdophosphate in the sample zone. Finally, the concentration of phosphate is obtained by difference. Silicate and phosphate are determined in boiler water at power plants; 60-120 samples h−1 be analyzed. Determination ranges are 0.05-22mg l−1 for silicate and 0.1-24 mg l−1 for phosphate. Relative standard deviations for metasilicate and orthophosphate were ≤1.2 and 1.3%, respectively. Recovery ranges of silicate and phosphate in the samples are 98–103%.
Flow injection, Phosphate, Silicate, Simultaneous determination, Power plant, Boiler water
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【期刊论文】フローインジェクション/固定化酵素分光光度法による尿中硫酸抱合胆汁酸の測定法の開発
李永生, 高秀峰, 軽部征夫
BUNSEKI KAGAKU Vol.52, No.3, pp. 195-200 (2003),-0001,():
-1年11月30日
本論文は,新規酵素胆汁酸硫酸スルフェターゼ(BSS)とWST-5 を用いて,迅速,簡便,高感度な硫酸抱合胆汁酸(SBA)のFIA/分光光度分析法を開発した。測定原理は次のとおりである。SBA はBSS により脱硫酸化し,3β-ヒドロキシステロイドが生成する。この3β-ヒドロキシステロイドが3β-ヒドロキシステロイドデヒドロゲナーゼ(3β-Hydroxysteroid dehydrogenase:3β-HSD)の触媒作用下でNAD+と反応し,NADH を生成する。次にジアホラーゼによりWST-5 がNADH と反応して水溶性のホルマザンブルーを生成する。この生成物は,550nm に吸収極大があるので,その吸光度によってSBA を定量した。本法は分析速度が15検体/h,相対標準偏差は1~5.4% であり,測定の範囲は1~75μM であった。
sulfated bile acid, water soluble tetrazolium blue-5, flow injection analysis, spectrophotometry, immobilized enzyme.,
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李永生, Yong-Sheng LI*† and Yi-Ling DONG**
ANALYTICAL SCIENCES MAY 2004, VOL. 20, 831-836,-0001,():
-1年11月30日
Based on a flow-injection spectrophotometry, an automatic analytical method for determination of ppb-level chloride-ion has been established. By use of this method, a novel FIA method for the determination of SBAER performance has also been developed. In this paper, the effects of concentration, dosage, and flow rate of the regenerant on BEC of SBAER were first investigated dynamically by the FIA method. In addition, the flow rate of the sample water and the temperature of the ion exchange resin were also examined. The optimum conditions were obtained: the volume of the regenerant (sodium hydroxide) was 50 mL (0.15g resin), and its concentration was 3% (w/v); the volumetric flow rates of the regenerant and the sample water were 0.5ml/min (4.3m/h) and 1.5ml/min (13 m/h), respectively. The exchanging temperature was 25±5℃. The method is characterized by the use of a micro resin-column, shorter testing cycle, easy operation, and high reproducibility. The proposed method is approximately 30 times more efficient than the manual method, and it can be used for the exchange performance comparison of various SBAER.
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李永生, Yong-Sheng LI, *† Cheng-Xia XING, ** and Li-Ling YANG**
ANALYTICAL SCIENCES MARCH 2005, VOL. 21, 273-279,-0001,():
-1年11月30日
A new method for the rapid determination of trace-level sodium ion based on flow-injection analysis (FIA) and an ion selective electrode (ISE) is proposed. Various effects on the sensitivity of the method such as the flow rate and alkalinity of the carrier, sampling volume, temperature, length of reaction coil, length and thickness of alkalization tube, concentration of the alkalizing reagent etc. were investigated. The optimum conditions were ascertained. The method showed good linearity in the concentration ranges of 0.5-10μg L-1 and 10-100μg L-1, and could deal with 40-50 samples per hour. The consumption of the sample is only 0.80mL per time. The relative standard deviation was 0.55%, and the recovery range was 98-103%. By designing a hermetically sealed single-line FIA-ISE manifold, a problem removing the interference of sodium ion from air could be solved, and automatic alkalization of the sample was realized. This method has been used for successfully determining the trace-level sodium ions in the water-steam system at fossil power plants
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