卢一平
长期从事泌尿外科,肾脏移植及男科学的临床、科研及教学工作,尤其在泌尿系统肿瘤基础和临床研究、泌尿系先天畸形诊治、腔道泌尿外科、男性生殖调控、男性性功能障碍诊治、移植免疫以及肾脏移植临床等方面有所特长。
个性化签名
- 姓名:卢一平
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学术头衔:
博士生导师
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学科领域:
外科学
- 研究兴趣:长期从事泌尿外科,肾脏移植及男科学的临床、科研及教学工作,尤其在泌尿系统肿瘤基础和临床研究、泌尿系先天畸形诊治、腔道泌尿外科、男性生殖调控、男性性功能障碍诊治、移植免疫以及肾脏移植临床等方面有所特长。
卢一平,1982年毕业于四川医学院医学系,获学士学位。1984年9月-1989年6月在华西医科大学医学院泌尿外科攻读硕士、博士研究生,分获医学硕士和医学博士学位。1989年9月-1991年9月赴德国萨尔大学医学院泌尿外科任博士研究生及客座医生,获德国医学博士学位。1991年10月起历任四川大学华西医院(原华西医科大学附属第一医院)泌尿外科讲师、主治医师、副教授、教授。长期从事泌尿外科及肾脏移植临床、科研及教学工作,尤其在泌尿系统肿瘤基础和临床研究、泌尿系先天畸形诊治、腔道泌尿外科、男性生殖调控、移植免疫以及肾脏移植临床等方面有所特长。
现任国务院高校学科同行评议组成员,国家科学技术奖励评审专家,教育部重点学科评审专家组成员,卫生部同行评议专家,国家自然科学基金委生命科学部评审专家,教育部留学回国人员科研启动基金评审专家,四川省卫生厅学术带头人;国际泌尿外科协会会员,国际器官移植协会会员,中华医学会器官移植专业委员会委员,中国透析移植研究会委员,四川省泌尿外科专业委员会副主任委员、四川省男科学专委会主任委员、四川省器官移植专业委员会副主任委员、四川省腔道泌尿外科学组副组长、成都市泌尿外科专业委员会副主任委员;中华医学会器官移植专业委员会医疗事故鉴定专家,北京大学医学部泌尿外科医师培训学院专家组成员,四川省高级人民法院司法鉴定特聘专家,四川省、成都市医学会医疗事故鉴定专家组成员,四川省、成都市计划生育事故鉴定专家组成员,成都市基本医疗保险评审专家。
担任《中华器官移植杂志》编委,《肾脏病、透析及肾移植杂志》编委,《国外医学:器官移植分册》编委,《国外医学:移植与血液透析分册》编委,《华西医学》编委,《现代泌尿外科杂志》编委,《移植杂志》编委,《生物医学工程学杂志》编委,《Current Opinion in Organ Transplantation Chinese Edition》编委,《中国肾友》编委,《生命之花》编委。
卢一平教授长期从事泌尿外科,肾脏移植及男科学的临床、科研及教学工作,尤其在泌尿系统肿瘤基础和临床研究、泌尿系先天畸形诊治、腔道泌尿外科、男性生殖调控、男性性功能障碍诊治、移植免疫以及肾脏移植临床等方面有所特长。已发表研究论文117篇,其中在国外专业杂志发表26篇;主编及参编专著13部。获包括国家自然科学基金重大项目分题在内的国家部委及省级以上纵向科研课题13项;获四川省科技进步一等奖、三等奖各一项,成都市科技进步三等奖一项,四川省卫生厅科技进步二等奖一项。
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成果数
13
【期刊论文】Study of Donor-Specific Antigens in Inducing Tolerance and Enhancing Graft Survival
卢一平, Y.P. Lu, J. Wang, Y.R. Yang, and X.D. Tang
Transplantation Proceedings, 31, 846 (1999),-0001,():
-1年11月30日
IT HAS been well proven that donor-specific antigens (DS-Ags) could induce immunologic tolerance of recipients and prolong allograft survival. But the detailed mechanisms are still unclear. The effect of the DS-Ags is influenced by some factors, particularly the species, the histocompatibility barries, the different sources of the DSAgs, the different pathways that the DS-Ags were given, the dose and timing of DS-Ags infusion, the immunoreactivity of the recipient to DS-Ags, etc. In this study, different DS-Ags and different pathways through which the DS-Ags were given were compared.
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【期刊论文】Human-Pig Spleen Transplantation Leading to High Level of Chimerism
卢一平, Y. Li, K. Wang, J. Cheng, F. Li, Y. Ma, and Y. Yang
Transplantation Proceedings, 32, 1103-1104 (2000),-0001,():
-1年11月30日
ONE OF the serious problems in allogenic clinical transplantation is the shortage of grafts. The pig is now widely accepted as the most appropriate candidate for xenotransplantation.1 It is very important to establish a large animal model to mimic pig-to-human xenotransplantation and to evaluate the donor-recipient interaction during the whole course in vivo. Our previous study showed that human bone marrow and spleen cells could survive in recipient pig for a long time after transplant. In this study, human spleen or spleen tissue was transplanted into pig to establish a higher level of chimerism. Therefore, it is possible to observe the interaction of human against pig, and it might be possible to develop xenogenic graft-versushost disease (xGVHD).
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【期刊论文】A New Rat Model of Transplant Arteriosclerosis Accelerated by Ischemia/Reperfusion Injury
卢一平, Y.P. Lu, W.G. Chen, I. Wang, and Y.P. Li
Transplantation Proceedings, 35, 184-186 (2003),-0001,():
-1年11月30日
ORGAN transplantation has become a realistic treatment for patients with end-stage organ failure.1,2 However, despite improvements in diagnosis and treatment of acute rejection, the consequences of chronic rejection, namely transplant arteriosclerosis, remain major obstacles to long-term survival of most solid organ allografts.3,4 Several animal models have been developed to investigate the mechanisms of the chronic rejection.5-7 However, most of these models have the disadvantages of requiring difficult operative skills, ensuring high rates of postoperative complications and host mortality as well as requiring a long time of observation. The present study was designed to investigate the feasibility of using SD and Wistar rats to design a new rat model of transplant arteriosclerosis and to accelerate the process by enhancing the ishemia/reperfusion (IR) injury.
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卢一平, M.Z. Liang, Y.P. Lu, F. Nan, Q. Yu, Y.P. Qin, and Y.G. Zou
Transplantation Proceedings, 36, 2065-2067 (2004),-0001,():
-1年11月30日
MYCOPHENOLIC acid (MPA) is the active metabolite of mycophenolate mofetil (MMF). Because of its immunosuppressive properties, the drug has now been widely used in immunosuppressive protocols after solid organ transplantation and for autoimmune diseases. The purpose of this study was to investigate the pharmacokinetics of MPA after single and multiple oral administrations of the pro-drug of MPA (MMF), in Chinese renal transplant recipients.
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卢一平, Y.P. Lu, B. Lin, M.Z. Liang, L. Wang, F. Nan, Q. Yu, and K.S. Tang
Transplantation Proceedings, 36, 2079-2081 (2004),-0001,():
-1年11月30日
MYCOPHENOLATE mofetil (MMF) is widely used in organ transplantation combined with other immunosuppressants to prevent acute rejection. 1-3 Because MMF can produce side effects, especially hematological and/or gastrointestinal toxicity, 1-5 therapeutic monitoring is becoming mandatory. Furthermore, there is no study to show the characteristics of MMF pharmacokinetics (PK) in Chinese patients. This study was designed to investigate the relationship between clinical events and the PK of mycophenolic acid (MPA) in Chinese kidney transplant recipients.
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卢一平, HAO ZENG, * QI WU, † HONG LI, * QIANG WEI, * YIPING LU, * XIANG LI, * FANG WANG, † FUJUN ZHAO, * ZHENGYU DING, ‡ AND YURU YANG*
Journal of Andrology, Vol. 26, No.2, March/April 2005,-0001,():
-1年11月30日
To screen different combinations of prostate-specific membrane antigen (PSMA) promoter/enhancer with the strongest transcriptional activity in prostate-specific cells, we used PSMA regulatory elements to control specific expression of the target gene in gene therapy of prostate adenocarcinoma. PSMA promoter and enhancer DNA sequences were amplified from the LNCaP human prostate cancer cell line by polymerase chain reaction, then recombinant plasmids of the enhanced green fluorescent protein (EGFP: pEGFP-PSMAPro, pEGFP-PSMAE-P, pEGFP-PSMAE(r)-P, pEGFPPSMA E(d)-P, and pEGFP-PSMAE(t)-P) were constructed with molecular clonal techniques. At the same time, all experimental cell lines were analyzed for the expression of PSMA with the use of PSMA monoclonal antibody and the ABC immunohistochemical assay kit. After plasmids were transfected via liposome, we observed the expression of the reporter gene (EGFP) under a fluorescent microscope and compared the different levels of EGFP expression with reverse transcriptase polymerase chain reaction and flow cytometry so that we could choose the one with the highest transcriptional activity. Only the LNCaP cell line expressed PSMA positively with immunohistochemical stain. The PSMA promoter/enhancer had transcriptional activity in PSMA(1) cell lines and no activity in PSMA(2) cell lines. PSMAE-P achieved the strongest activity in different PSMA promoter/ enhancer combinations. We confirmed the specific expression of PSMA in prostate cells again. Similarly, transcriptional activity of the PSMA promoter/enhancer was prostate specific. PSMAE-P achieved the strongest transcriptional activity among PSMA promoter/ enhancer combinations, which could be used in advanced research for tissue-specific treatment.
Adenocarcinoma,, regulatory element.,
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【期刊论文】Assessment of Glomerular Filtration Rate in Renal Transplant Patients Using Serum Cystatin C
卢一平, H. Xu, Y. Lu, D. Teng, J. Wang, L. Wang, and Y. Li
Transplantation Proceedings, 38, 2006-2008 (2006),-0001,():
-1年11月30日
Aim. Serum cystatin C (SCysC) has been proposed as a better marker of glomerular filtration rate (GFR) than serum creatinine (Scr). However, few data are available in renal transplant patients, especially, during the early postoperative phase. Methods. Thirty-nine renal transplant patients (22 men/17 women) were recruited for determination of SCysC and Scr before operation, at 1 week and at 4 weeks after operation. SCysC was determined by particle-enhanced turbidimetric immunoassay. Creatinine clearance (Ccr) was calculated using the Cockcroft-Gault formula. Results. SCysC and Scr levels significantly decreased with the recovery of allograft function. SCysC showed a significant correlation with Scr and Ccr. The relationship between SCysC and Scr showed a positive correlation (r=.849 preoperation, and r=.940 postoperation). The relationship between SCysC and Ccr revealed a negative correlation (r=.857 preoperation, and r=.876 postoperation). At the Ccr level of 50 to 80mL/min/1.73m2, the correlation between SCysC and Ccr (r=.778) was significantly better than that between Scr and Ccr (r=.553; P=.032). The concentration of SCysC was not affected by age, gender, height, body weight, hemoglobin, serum protein, glucose, or mycophenolate mofetil or azathioprine dosage. However, corticosteroids slightly increased the level of SCysC and cyclosporine (CsA) decreased it. The area under the curve of the receiver operating characteristic curve for SCysC and Scr are 0.964 and 0.915, respectively (P<.05). Conclusion. Although the concentration may be slightly influenced by prednisolone and CsA, SCysC is more sensitive than Scr to detect early and moderate deterioration of GFR in adult renal transplant recipients.
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卢一平, G. Cao, Y. Lu, R. Gao, Y. Xin, D. Teng, J. Wang, L. Wang, and Y. Li
Transplantation Proceedings, 38, 2234-2236 (2006),-0001,():
-1年11月30日
Introduction. We sought to investigate whether there was a difference between cyclosporine (CsA) and mycophenolate mofetil (MMF) to affect the expression of Fractalkine/ CX3CR1 in chronic allograft nephropathy (CAN). Methods. The Sprague-Dawley Wistar rat accelerated kidney sclerosis model was performed as modified from the procedure of Kamada. Recipients were divided into three oral treatment groups (each group n=8): group A was CsA 10mg/kg•d for 10 days followed by vehicle; group B was CsA 10mg/kg•d for 10 days followed by CsA 6mg/kg•d; group C was CsA 10mg/kg•d for 10 days followed by MMF 20mg/kg•d. Pathological changes graded according to Banff 97 Standards were observed at 4, 8, and 12 weeks posttransplantation. The immunohistochemistry and quantitative real-time fluorescence polymerase chain reaction (PCR) were used to assess the distribution and expression of Fractalkine/CX3CR1 in the grafted kidney. Results. Fractalkine/CX3CR1 were mostly expressed in the tubulointerstitium and tubular epithelial cell basolateral membrane. A proportion of the vessel showed positive staining for Fractalkine/CX3CR1, occasionally in glomerular parietal wall cells. The expression of Fractalkine/CX3CR1 in grafted kidneys at all the time points was significantly less in the MMF than in the CsA group or the control group (P<.05). Real-time fluorescence quantitative PCR revealed similar outcomes as immunohistochemistry. The expression of Fractalkine coincided with CX3CR1. Conclusion. Fractalkine/CX3CR1 may play an important role in the development of interstitial fibrosis in CAN. Different immunosuppresants have various effects on expression of the Fractalkine/CX3CR1.
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卢一平, D. Teng, Y. Lu, R. Gao, Y. Xin, G. Cao, X. Li, L. Wang, J. Wang, and Y. Li
Transplantation Proceedings, 38, 2164-2167 (2006),-0001,():
-1年11月30日
Aims. Cytoprotective genes have shown to display potent anti-inflammatory and antiapoptotic functions in endothelial and smooth muscle cells. We investigated whether cytoprotective genes, especially A20, were involved in mycophenolate mofetil (MMF)’s ability to ameliorate transplant arteriosclerosis in an experimental chronic rejection model. Methods. Sprague-Dawley rat renal grafts were orthotopically transplanted into Wistar rats following the procedure of Kamada with our modification. The recipients were divided into three oral treatment groups: (1) vehicle group (cyclosporine [CsA] 10mg/kg•d×10d followed by vehicle), (2) CsA group (CsA 10mg/kg•d×10dd followed by CsA 6mg/kg • d), (3) MMF group (converted from CsA 10mg/kg•d×10d toMMF20mg/kg•d on day 11). At the same time points after transplantation, the rats were sacrificed to harvest the renal allografts. The expression of four cytoprotective genes, A20, heme oxygenase (HO)-1, Bcl-2, and Bcl-XL, was analyzed by quantitative reverse-transcriptase polymerase chain reaction and immunohistochemistry. Results. The four-cytoprotective genes were all detected in rat kidney allografts undergoing chronic allograft nephropathy. The expression of A20 in grafted kidneys was significantly higher in the MMF than in the CsA or the vehicle group (P<.01). There was no significant difference between the CsA and the MMF groups in the expression of HO-1, Bcl-2, and Bcl-XL. Conclusions. We demonstrated that MMF improved the expression of A20 in rat kidney allografts undergoing chronic allograft nephropathy. The correlation between MMF and A20 provide an explanation for the mechanism by which MMF ameliorates transplant arteriosclerosis in an experimental animal model of chronic rejection.
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卢一平, G. Cao, Y. Lu, R. Gao, Y. Xin, D. Teng, J. Wang, and Y. Li
Transplantation Proceedings, 38, 1998-2000 (2006),-0001,():
-1年11月30日
Aim. Fractalkine/CX3CR1 system may contribute to the pathogenesis of renal allograft chronic rejection (CR). Vascular endothelial growth factor (VEGF) is an endothelial mitogen, which shows increased expression in inflammation and vasculopathy. This study sought describe the expression and distribution of Fractalkine/CX3CR1 and VEGF, and their relationship to human renal allograft CR. Methods. Renal tissue from 10 patients with CR was examined for Fractalkine/CX3CR1 and VEGF protein by immunohistochemistry for comparison with patients displaying hyperacute rejection (n=10), acute rejection (n=10), and normal kidneys (n=10). All patients were selected based upon histologically proven diagnoses between 1992 and 2003. Results. Immunohistochemistry revealed that Fractalkine/CX3CR1 were mostly expressed in the tubulointerstitium and tubular epithelial cell basolateral membrane. Some vessels showed positive staining for Fractalkine/CX3CR1 as well as occasionally glomerular parietal wall cells. Among the CR group, VEGF was mostly expressed in tubular epithelium and the tubulointerstitium. A proportion of glomeruli and vessels had positive staining for VEGF, which was up-regulated most strikingly in the interstitial compartment in CR. There was markedly increased expression of Fractalkine/CX3CR1 and VEGF protein in the interstitium of the CR compared with other groups (P<.05). VEGF colocalized with the expression of Fractalkine/CX3CR1. Conclusion. Fractalkine/CX3CR1 and VEGF may play an important role in the development of interstitial fibrosis via mononuclear cell-induced cytokine production and myofibroblast stimulation in CR. Further studies are necessary to identify the role in the pathogenesis of CR.
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