目的 建立人宫颈癌细胞株并观察其生物学特性。方法 从宫颈鳞癌组织取材，体外培养，建株后进行细胞形态学观察，分析其生物学特性和细胞遗传学变化，检测裸鼠接种CS1213后的成瘤性和p53、bcl-2、Ki67基因表达。结果 新建的CS1213细胞株在体外已传代170次。细胞呈圆形，悬浮生长，胞浆内有大量的张力原纤维，群体倍增时间为25h，集落形成率为19%，具有超二倍体核型，染色体主干系为80～88。细胞无支原体污染，有特异性的乳酸脱氢酶同工酶谱，p53、bcl-2和Ki67基因高表达，CS1213细胞接种Balb/c裸鼠，可形成移植瘤，并具有原发宫颈癌的组织学特性。结论 新建立的CS1213细胞具有人宫颈鳞状上皮细胞癌的特点，是研究肿瘤细胞浸润和转移的理想细胞模型。

Rat fibroblasts were infected with a retroviral vector containing the cDNA for rat tyrosine hydroxylase [TH; tyrosine 3-monooxygenase; L-tyrosine, tetrahydropteridine: oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2]. A THpositive clone was identified by biochemical assay and immunohistochemical staining. When supplemented in vitro with pterin cofactors required for TH activity, these cells produced L-dopa and released it into the cell culture medium. Uninfected control cells and fibroblasts infected with the TH vector were grafted separately to the caudate of rats with unilateral 6-hydroxydopamine lesions of the nigrostriatal pathway. Only grafts containing TH-expressing fibroblasts were found to reduce rotational asymmetry. These results have general implications for the application of gene therapy to human neurological disease and specific implications for Parkinson disease.

为了观察外源性端粒酶逆转录酶基因（hTERT）在人脐静脉血管内皮细胞（HIJVEc）的表达及对细胞功能和生长的影响。采用逆转录病毒载体转导的方法，将hTERT基因转入HU-VEC。检测基因转导后内皮细胞端粒酶的活性和生物学特性。结果发现hTERT转导后细胞端粒酶表达阳性，未转导的亲代细胞为阴性；转导细胞的体外生存时间延长但未永生化，而内皮细胞黏附分子表达的功能未受影响。

Objectives: To compare tumor necrosis factor receptor 1 (TNFR1) protein expression in women with unexplained early spontaneous abortion (UESA) and normal pregnancy. Methods: In a prospective study, 62 women with UESA and 60 with normal pregnancy were studied. Decidual membrane TNFR1 was detected by flow cytometry and immunohistochemistry, and serum soluble TNFR1 were by ELISA. Statistical analyses of resulting data employed the student's t test, the Fisher's Exact, and the nonparametric Wilcoxon test. Results: The percentage of membrane tumor necrosis factor receptor 1 (mTNFR1) positive decidual cells was 16.42F7.1 for women with UESA and 12.47F5.3 for women with normal pregnancy (pb0.05). The number of mTNFR1 positive cells was more in decidual stromal and vessel endothelial cells in women with UESA, and serum soluble tumor necrosis factor receptor 1 (sTNFR1) concentration was significantly higher than in women with normal pregnancy (554.56F126.7pg/ml vs. 175.3F52.4pg/ml; pb0.001). Conclusion: The overexpression of TNFR1 may contribute to the development of ESA.

目的：构建抗人肝细胞癌单链抗体基因，并在大肠杆菌中表达。方法：采用RT-PCR法扩增抗体重链和轻链可变区基因，用（Gly4Ser）3肽段连接VH基因和VL基因，构建克隆载体，将测序完全正确的ScFv基因克隆到分泌性表达载体pCANTAB5E中诱导表达转化的TGl和HB2151细胞。用SDS-PAGE和免疫组化方法分析检测表达产物。结果：DNA测序证实ScFv基因结构正确，可在大肠杆菌中成功表达，游离ScFv相对分子质量大约为30ku，与HC-108，HepG-2和SMMC-7721肝癌细胞有特异性的结合反应。结论：重组制备的抗人HCD78分子单链抗体在人肝癌的基础和临床研究中具有潜在的应用价值。