马力耕
植物细胞信号转导、植物发育生物学和功能基因组学。
个性化签名
- 姓名:马力耕
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 国家杰出青年科学基金获得者
- 职称:-
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学科领域:
植物学
- 研究兴趣:植物细胞信号转导、植物发育生物学和功能基因组学。
马力耕,1987毕业于河北师范大学生物学系,获学士学位;1990毕业于河北师范大学生物学系植物生理学专业,获硕士学位;1997 毕业于中国农业大学生物学院生物化学专业,获博士学位。2000年8月至2004年12月在耶鲁大学分子、细胞和发育生物学系做博士后。1998年8月至2000年8月担任河北师范大学教授、博士生导师、分子细胞生物学研究室主任。是国家自然科学杰出青年基金获得者;教育部全国高校优秀教师资助计划获得者;教育部全国高校骨干教师资助计划获得者;教育部、霍英东教育基金会全国高校青年教师研究奖获得者;中国植物生理学会优秀论文一等奖获得者;河北省有突出贡献的中青年专家;教育部科技进步二等奖获得者;河北省科技进步一等奖获得者;国际人类前沿科学计划(Human Frontier Science Program)研究项目获得者(long-term fellow);北京大学东盛科学论文奖获得者;研究结果被评为2002年度"中国高等学校十大科技进展"之一;曾主持国家自然科学杰出青年基金项目、国家自然科学基金青年项目、国家自然科学基金项目、863重大专项子课题。现主持863重大专项课题。
近10多年的研究工作主要集中在两个研究领域:植物细胞信号转导、植物发育生物学和功能基因组学。其中前者主要工作包括证实细胞外钙调素参与植物花粉萌发和花粉管伸长调控,G蛋白也参与调控这些过程并可能参与细胞外钙调素信号转导等工作。经过几年的工作,一方面为细胞外钙调素的研究找到了好的实验体系:植物花粉体系,更重要的是逐渐建立起了细胞外钙调素研究的理论体系。期间发表了我国学者在国内完成的第一篇学科最高刊物(The Plant Cell)论文。后者包括利用基因芯片分析技术研究植物光控发育等。
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马力耕, Edward Kraft, Sophia L. Stone, Lingeng Ma, Ning Su, Ying Gao, On-Sun Lau, Xing-Wang Deng, and Judy Callis*
Plant Physiology, December 2005, Vol. 139, pp. 1597-1611,-0001,():
-1年11月30日
Attachment of ubiquitin to substrate proteins is catalyzed by the three enzymes E1, E2 (ubiquitin conjugating [UBC]), and E3 (ubiquitin ligase). Forty-one functional proteins with a UBC domain and active-site cysteine are predicted in the Arabidopsis (Arabidopsis thaliana) genome, which includes four that are predicted or shown to function with ubiquitin-like proteins. Only nine were previously characterized biochemically as ubiquitin E2s. We obtained soluble protein for 22 of the 28 uncharacterized UBCs after expression in Escherichia coli and demonstrated that 16 function as ubiquitin E2s. Twelve, plus three previously characterized ubiquitin E2s, were also tested for the ability to catalyze ubiquitination in vitro in the presence of one of 65 really interesting new gene (RING) E3 ligases. UBC22, UBC19-20, and UBC1-6 had variable levels of E3-independent activity. Six UBCs were inactive with all RINGs tested. Closely related UBC8, 10, 11, and 28 were active with the largest number of RING E3s and with all RING types. Expression analysis was performed to determine whether E2s or E3s were expressed in specific organs or under specific environmental conditions. Closely related E2s show unique patterns of expression and most express ubiquitously. Some RING E3s are also ubiquitously expressed; however, others show organspecific expression. Of all the organs tested, RING mRNAs are most abundant in floral organs. This study demonstrates that E2 diversity includes examples with broad and narrow specificity toward RINGs, and that most ubiquitin E2s are broadly expressed with each having a unique spatial and developmental pattern of expression.
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马力耕, Derek J. Gingerich‡, Jennifer M. Gagne‡, Donald W. Salter‡§, Hanjo Hellmann¶‖, Mark Estelle¶, Ligeng Ma‡‡, and Richard D. Vierstra‡**
Vol. 280, No.19, Issue of May 13, pp. 18810-18821, 2005,-0001,():
-1年11月30日
Selective modification of proteins by ubiquitination is directed by diverse families of ubiquitin-protein ligases (or E3s). A large collection of E3s use Cullins (CULs) as scaffolds to form multisubunit E3 complexes in which the CUL binds a target recognition subcomplex and the RBX1 docking protein, which delivers the activated ubiquitin moiety. Arabidopsis and rice contain a large collection of CUL isoforms, indicating that multiple CUL-based E3s exist in plants. Here we show that Arabidopsis CUL3a and CUL3b associate with RBX1 and members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form BTB E3s. Eighty genes encoding BTB domain-containing proteins were identified in the Arabidopsis genome, indicating that a diverse array of BTB E3s is possible. In addition to the BTB domain, the encoded proteins also contain various other interaction motifs that likely serve as target recognition elements. DNA microarray analyses show that BTB genes are expressed widely in the plant and that tissue-specific and isoform-specific patterns exist. Arabidopsis defective in both CUL3a and CUL3b are embryo-lethal, indicating that BTB E3s are essential for plant development.
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马力耕, Sujuan Cui, Xiaoqiang Guo, Fang Chang, Yanwei Cui, Ligeng Ma, Ying Sun‡, and Daye Sun§
Vol. 280, No.36, Issue of September 9, pp. 31420-31427, 2005,-0001,():
-1年11月30日
Calmodulin, a highly conserved protein family that has long been well known as an intracellular calcium sensor, was identified in the culture medium and cell walls of Arabidopsis thaliana suspension-cultured cells by immunoblotting assay. A promotion effect by applying exogenous purified calmodulin and an inhibition effect by the addition of anti-calmodulin anti-serum or calmodulin antagonist to the medium on proliferation of suspension cells were found by monitoring incorporation of [methyl-3H]thymidine into nuclear DNA. Radioligand binding analysis with 35S-labeled calmodulin indicated the presence of specific, reversible, and saturable calmodulin binding sites on the surface of both A. thaliana suspension-cultured cells and its protoplasts; among them at least one is on the surface of Arabidopsis protoplasts, with the Kd~9.2 nM, and two are on the out-surface of Arabidopsis suspension-cultured cells, with Kd values of~47.5 and 830 nM. Chemical crosslinking of 35S-labeled calmodulin to protoplasts revealed 117- and 41-kDa plasma membrane proteins specifically bound to calmodulin, whereas cross-linking with intact suspension-cultured cells verified more calmodulin binding proteins which might be cell wall-associated in addition to membrane-localized. Taking together, our data provide first evidence for the presence of apoplastic calmodulin receptor-like binding proteins on the cell surface of Arabidopsis suspension-cultured cells, which strongly supports our previous idea that apoplastic calmodulin functions as a peptide signal involved in regulation of cell growth and development.
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马力耕, Zhong-lin Shang , , Li-geng Ma , Hai-lin Zhang , Rui-rong He , Xue-chen Wang , Su-juan Cui and Da-ye Sun
Plant Cell Physiol. 46 (4): 598-608 (2005),-0001,():
-1年11月30日
Confocal laser scanning microscopy (CLSM) and whole-cell patch-clamp were used to investigate the role of Ca2+ influx in maintaining the cytosolic Ca2+ concentration ([Ca2+]c) and the features of the Ca2+ influx pathway in germinating pollen grains of Lilium davidii D. [Ca2+]c decreased when Ca2+ influx was inhibited by EGTA or Ca2+ channel blockers. A hyperpolarization-activated Ca2+- permeable channel, which can be suppressed by trivalent cations, verapamil, nifedipine or diltiazem, was identified on the plasma membrane of pollen protoplasts with wholecell patch-clamp recording. Calmodulin (CaM) antiserum and W7-agarose, both of which are cell-impermeable CaM antagonists, lead to a [Ca2+]c decrease, while exogenous purified CaM triggers a transient increase of [Ca2+]c and also remarkably activated the hyperpolarization-activated Ca2+ conductance on plasma membrane of pollen protoplasts in a dose-dependent manner. Both the increase of [Ca2+]c and the activation of Ca2+ conductance which were induced by exogenous CaM were inhibited by EGTA or Ca2+ channel blockers. This primary evidence showed the presence of a voltage-dependent Ca2+-permeable channel, whose activity may be regulated by extracellular CaM, inpollen cells.
Cytosolic calcium-Extracellular CaM-Hyperpolarization-activated Ca2+, permeable channel-Lilium davidii-Pollen grain.,
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马力耕, Yan-Yun Pan , , Xin Wang , Li-Geng Ma and Da-Ye Sun , *
Plant Cell Physiol. 46 (10): 1657-1665 (2005),-0001,():
-1年11月30日
The phosphatidylinositol-specific phospholipase C (PIPLC) activity is detected in purified Lilium pollen protoplasts. Two PI-PLC full length cDNAs, LdPLC1 and LdPLC2, were isolated from pollen of Lilium daviddi. The amino acid sequences for the two PI-PLCs deduced from the two cDNA sequences contain X, Y catalytic motifs and C2 domains. Blast analysis shows that LdPLCs have 60–65% identities to the PI-PLCs from other plant species. Both recombinant PI-PLCs proteins expressed in E. colicells show the PIP2-hydrolyzing activity. The RT-PCR analysis,shows that both of them are expressed in pollen grains, whereas expression level of LdPLC2 is induced in germinating pollen. The exogenous purified calmodulin (CaM) is able to stimulate the activity of the PI-PLC when it is added into the pollen protoplast medium, while anti-CaM antibody suppresses the stimulation effect caused by exogenous CaM. PI-PLC activity is enhanced by G protein agonist cholera toxin and decreased by G protein antagonist pertussis toxin. Increasing in PI-PLC activity caused by exogenous purified CaM is also inhibited by pertussis toxin. A PI-PLC inhibitor, U-73122, inhibited the stimulation of PIPLC activity caused by cholera toxin and it also leads to the decrease of [Ca2+]cyt in pollen grains. Those results suggest that the PPI-PLC signaling pathway is present in Lilium daviddi pollen, and PI-PLC activity might be regulated by aheterotrimeric G protein and extracellular CaM.
cDNA cloning-Extracellular calmodulin (, CaM), -Heterotrimeric G protein-Lilium daviddi pollen-Phosphatidylinositol-specific phospholipase C.,
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马力耕, Jian-Xun Feng, Di Liu, , Yi Pan, Wei Gong, Li-Geng Ma, Jing-Chu Luo, Xing Wang Deng, and Yu-Xian Zhu, *
Plant Molecular Biology (2005) 59: 853-868,-0001,():
-1年11月30日
AP2/EREBP transcription factors (TFs) play functionally important roles in plant growth and development, especially in hormonal regulation and in response to environmental stress. Here we reported verification and correction of annotation through an exhaustive cDNA cloning and sequence analysis performed on 145 of 147 gene family members. A RACE analysis performed on genes with potential in-frame up-stream ATG codon resulted in identification of At2g28520 as an authentic AP2/EREBP member and corrected ORF annotations for three other members. A further phylogenetic analysis of this updated and likely complete family divided it into three major subfamilies. The expression patterns of the AP2/EREBP family members among the 11 organ or tissue types were examined using an oligo microarray and their hormonal and environmental responsiveness were further characterized using cDNA custom macroarrays. These etailed expression profile results provide strong support for a role for AP2/EREBP family members in development and in response to environmental stimuli, and a foundation for future functional analysis of this gene family.
AP2/, EREBP,, gene family,, HMM,, microarray,, trans, c, r, i, p, t, ion factor
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【期刊论文】Arabidopsis FHY1 Protein Stability Is Regulated by Lightvia Phytochrome A and 26S Proteasome1
马力耕, Yunping Shen, Suhua Feng, Ligeng Ma, Rongcheng Lin, Li-Jia Qu, Zhangliang Chen, Haiyang Wang, and Xing Wang Deng*
Plant Physiology, November 2005, Vol. 139, pp. 1234-1243,-0001,():
-1年11月30日
Phytochrome A (phyA) is the primary photoreceptor mediating responses to far-red light. Among the phyA downstream signaling components, Far-red Elongated Hypocotyl 1 (FHY1) is a genetically defined positive regulator of photomorphogenesis in far-red light. Both physiological and genomic characterization of the fhy1 mutants indicated a close functional relationship of FHY1 with phyA. Here, we showed that FHY1 is most abundant in young seedlings grown in darkness and is quickly downregulated during further seedling development and by light exposure. By using light-insensitive 35S promoter-driven functional β-glucuronidase-FHY1 and green fluorescent protein-FHY1 fusion proteins, we showed that this down-regulation of FHY1 protein abundance by light is largely at posttranscriptional level and most evident in the nuclei. The light-triggered FHY1 protein reduction is primarily mediated through the 26S proteasome-dependent protein degradation. Further, phyA is directly involved in mediating the light-triggered down-regulation of FHY1, and the dark accumulation of FHY1 requires functional pleiotropic Constitutive Photomorphogenic/De-Etiolated/Fusca proteins. Our data indicate that phyA, the 26S proteasome, and the Constitutive Photomorphogenic/De-Etiolated/Fusca proteins are all involved in the light regulation of FHY1 protein abundance during Arabidopsis (Arabidopsis thaliana) seedling development.
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马力耕, Yuling Jiaoa, Ligeng Ma, a, b, c, Elizabeth Strickland, a and Xing Wang Denga,
PLANT CELL 2005; 17; 3239-3256,-0001,():
-1年11月30日
Genome-wide 70-mer oligonucleotide microarrays of rice (Oryza sativa) and Arabidopsis thaliana were used to profile genome expression changes during light-regulated seedling development. We estimate that the expression of; 20% of the genome in both rice and Arabidopsis seedlings is regulated by white light. Qualitatively similar expression profiles from seedlings grown under different light qualities were observed in both species; however, a quantitatively weaker effect on genome expression was observed in rice. Most metabolic pathways exhibited qualitatively similar light regulation in both species with a few species-specific differences. Global comparison of expression profiles between rice and Arabidopsis reciprocal best-matched gene pairs revealed a higher correlation of genome expression patterns in constant light than in darkness, suggesting that the genome expression profile of photo morphogenesis is more conserved. Transcription factor gene expression under constant light exposure was poorly conserved between the two species, implying a faster-evolving rate of transcription factor gene expression in light-grown plants. Organ-specific expression profiles during seedling photo morphogenesis provide genome-level evidence for divergent light effects in different higher plant organs. Finally, overrepresentation of specific promoter motifs in root- and leaf-specific light-regulated genes in both species suggests that these cis-elements are important for gene expression responses to light.
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【期刊论文】A microarray analysis of the rice transcriptome and its comparison to Arabidopsis
马力耕, Ligeng Ma, , Chen Chen, Xigang Liu, Yuling Jiao, Ning Su, Lin Li, Xiangfeng Wang, Mengliang Cao, Ning Sun, Xiuqing Zhang, Jingyue Bao, Jian Li, Soren Pedersen, Lars Bolund, Hongyu Zhao, Longping Yuan, Gane Ka-Shu Wong, Jun Wang, Xing Wang Deng, and Jian Wang
Genome Research 15: 1274-1283,-0001,():
-1年11月30日
Arabidopsis and rice are the only two model plants whose finished phase genome sequence has been completed. Here we report the construction of an oligomer microarray based on the presently known and predicted gene models in the rice genome. This microarray was used to analyze the transcriptional activity of the gene models in representative rice organ types. Expression of 86% of the 41,754 known and predicted gene models was detected. A significant fraction of these expressed gene models are organized into chromosomal regions, about 100 kb in length, that exhibit a coexpression pattern. Compared with similar genome-wide surveys of the Arabidopsis transcriptome, our results indicate that similar proportions of the two genomes are expressed in their corresponding organ types. A large percentage of the rice gene models that lack significant Arabidopsis homologs are expressed. Furthermore, the expression patterns of rice and Arabidopsis best-matched homologous genes in distinct functional groups indicate dramatic differences in their degree of conservation between the two species. Thus, this initial comparative analysis reveals some basic similarities and differences between the Arabidopsis and rice transcriptomes.
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马力耕, 汪俏梅, 刘洋, 赵普庆, 马力耕**
自然科学进展。2005。15(7):776~781,-0001,():
-1年11月30日
系统素最初是指在番茄叶片中发现的与植物防卫反应相关的一种18个氨基酸的多肽信号,随着在茄科植物中其他相类似的多肽防卫信号的发现,现系统素被定义为茄科植物中具有调节防卫基因功能的一类多肽信号家族。近年来对系统素信号转导的研究取得了很大进展,不仅在系统素信号转导途径上,而且在系统素与其他信号转导途径的相互作用上均有了很大进展。文中介绍了系统素的信号转导途径以及系统素与其他信号转导途径,如与油菜素甾醇类激素(BRs)和紫外线-B(UV-B)信号转导途径之间的相互作用,并对这一领域的研究进行了展望。
系统素, 信号转导, 油菜素甾醇类, tBRI/, SR160, 茉莉酸
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