洪法水
生物化学与分子生物学,微量元素和重金属离子在生物体内的作用机制
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- 姓名:洪法水
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学术头衔:
博士生导师
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学科领域:
植物学
- 研究兴趣:生物化学与分子生物学,微量元素和重金属离子在生物体内的作用机制
洪法水,男,出生于1960.12,1984年本科毕业于安徽大学生物系,2000年博士毕业于中国科技大学化学系分析化学专业,1984-1992工作于安徽技术师范学院生物系,1993-1997工作于淮北煤炭师范学院生物系,现任苏州大学生命科学学院教授、博导。
研究方向:生物化学与分子生物学,微量元素和重金属离子在生物体内的作用机制。曾主持省部级科研项目4项,主持或主要参加国家自然科学基金项目3项,主持市厅级科研项目6项。获省科技进步四等奖2项,获市科技进步二等奖和三等奖各1项。获苏州大学陈金荣生命科学英才奖(科研成果奖)一等奖、苏鑫科研成果奖、周氏科研成果奖各1项。1999年以来在国内外SCI刊源上发表或已录用论文68篇(截止2007年)。1995年获“全国优秀教师称号”。
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【期刊论文】Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach
洪法水, HONG Fashui, WANG Xuefeng, LIU Chao, SU Guoxing, SONG Weiping, WU Kang, TAO Ye & ZHAO Guiwen
SCIENCE IN CHINA (Series B) Vol. 46 No.1 (2003) 42-50,-0001,():
-1年11月30日
It was studied by spectroscopy that PSII reaction center complex consisting of three polypeptides, D1, D2 and Cytb559, were purified from PSII particle of CeCl3 treated spinach. The results of the experiment show that Ce3+ could improve the growth of spinach, and accelerate electron transport of PSII particles. Of chl-a of UV-Vis spectrum of D1/D2/Cytb559 complex, Soret band was blue-shifted by 3 nm and Q band by 2 nm, respectively, and the fluorescence emission peak was blue-shifted by 5 nm in CeCl3-treated spinach compared with the one in control. By the extended X-ray absorption fine structure (EXAFS) spectroscopy methods, it has been found that Ce3+ is coordinated with 8 nitrogen atoms in the first coordination shell with Ce-N bond length of 0.253 nm, and Ce3+ with 6 oxygen atoms in the second coordination shell with Ce-O bond length of 0.32 nm. However, the secondary structure of D1/D2/Cytb559 complex by circular dichroism (CD)spectroscopy has no significant change after CeCl3 treated. It might be that Ce3+ binds to porphyrin rings of chlorophyll and oxygen of amino acid residue of polypeptide in D1/D2/Cytb559 complex,and then accelerates the primary reaction of PSII, intensifies function of P680+ primary electron donor of D1/D2/Cytb559, but there is little change in conformation of PSII reaction center complex.
Ce3+, ,, spinach,, D1/, D2/, Cytb559 complex,, photosynthesis,, spectra characteristic.,
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