唐建武
肿瘤病理学
个性化签名
- 姓名:唐建武
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学术头衔:
博士生导师
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学科领域:
肿瘤学
- 研究兴趣:肿瘤病理学
唐建武,男,1953年5月出生于辽宁省大连市,汉族,农工党党员,病理学教授,博士生导师,现任大连医科大学校长,国务院政府津贴获得者。
曾留学荷兰,并曾任大连医科大学基础医学部副主任、基础医学院院长、大连市卫生局副局长、大连医科大学副校长,自2002年3月开始担任大连医科大学校长。现兼任辽宁省政协常委,大连市政协副主席,卫生部高等医学教育学会常务理事,全国高等医药教材建设研究会常任理事,卫生部成人教育规划教材编委会主任委员,全国医学专科教材评审委员会副主任委员,辽宁省及大连市医学会、医师学会副会长,辽宁省及大连市病理学会副主任委员等职。
唐建武教授长期从事肿瘤病理学教学、科研和医疗工作。主持完成课题及获奖多项,其中“子宫肿瘤凝集素结合特性研究”获省政府科技进步三等奖;国家自然科学基金项目“子宫鞘糖脂及子宫凝集素结合特性在不同功能时期和肿瘤时期变化”,获国家教委科技进步三等奖;国家自然科学基金项目“小鼠肝癌细胞在淋巴道转移中粘着机制”;“白细胞介素-2抗肿瘤作用对肿瘤淋巴细胞亚群和肿瘤坏死因子的影响”等已获得成果。在国内外杂志上发表各类论文50余篇,其中一些为SCI和“美国生物学文摘”等摘编、引用。主编、副主编卫生部全国规划教材《病理学》、《临床医学导论》、《生物医学基础》等著作8部。曾多次获得省市级优秀科技工作者、市劳模等光荣称号和国家教委、国家人事部全国优秀留学回国人员称号。
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唐建武, Li Hou, Jan-Wu Tang, Xiao-Nan Cui, Bo Wang, Bo Song, Lei Sun
World J Gastroenterol 10 16(2004)2318-2322,-0001,():
-1年11月30日
AIM:In order to elucidate the molecular mechanism of lymphatic metastasis of hepatocarcinoma,we detected the difference of gene expression between mouse hepatocarcinoma cell lines Hca-F and Hca-P with different lymphatic metastasis potential.METHODS:cDNA of Hca-F cells was used as a tester and cDNA of Hca-P cells was used as a driver.cDNAs highly expressed in Hca-F cells were isolated by the suppression subtractive hybridization (SSH) method.The isolated cDNA was cloned into T/A cloning vector.The ligation products were transformed into DH5 α competent cells.Individual clones were randomly selected and used for PCR amplification.Vector DNA from positive clones was isolated for sequencing. RESULTS:There were 800 positive clones in amplified subtracted cDNA library.Random analysis of 160 clones with PCR showed that 95% of the clones contained 100-700 bp inserts.Analysis of 20 sequenced cDNA clones randomly picked from the SSH library revealed 4 known genes (mouse heat shock protein 84 ku,DNA helicase,ribosomal protein S13,ethanol induced 6 gene) and 3 expressed sequence tags (ESTs).Four cDNAs showed no homology and presumably represent novel genes.CONCLUSION:A subtracted cDNA library of differentially expressed genes in mouse heptocarcinoma cell lines with different lymphatic metastasis potential was successfully constructed with SSH and T/A cloning techniques.The library is efficient and lays a solid foundation for searching new lymphatic metastasis related genes.The expression of mouse heat shock protein gene, DNA helicase and other 4 novel gene may be different between mouse heptocarcinoma cell lines with different lymphatic metastasis potential.
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【期刊论文】高、低淋巴道转移能力小鼠肝癌细胞株抑制性消减杂交文库的构建
唐建武, 崔晓楠, 侯力, 宋波, 班丽英
中国肿瘤,2007,16(8):620~624,-0001,():
-1年11月30日
应用抑制性消减杂交(suppression subtracted hvh ridization, SSH)技术,分别构建2个不同淋巴道转移能力小鼠肝癌细胞株的SSH文库.高转移文库以高转移能力细胞株Hca-F为检测子(tester),同源的低转移能力细胞株Hca-P为驱赶子(driver):低转移文库则相反.随机筛选2个文库阳性克隆进行测序。并在GenBank数据库中进行l司源性比较。成动构建高、低淋巴道转移能力小鼠肝癌细胞株SSH文库,高、低转移文库中分别包含995个及967个阳性克隆:其中.95%的阳性克隆含有300bp-1OOObp不等的插入片段:随机测序显示,文库中含有已知的基凶。ESTs及与任何序列无例源性的新基凶片段。
抑制性消减杂交技术, 肝肿瘤, 肿瘤转移, 细胞株
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唐建武, Xiao-Nan Cui, Jian-Wu Tang, Li Hou, Bo Song, Li-Ying Ban
World J Gastroenterol 2006 November 14; 12 (42): 6893-6897,-0001,():
-1年11月30日
AIM: To identify genes differentially expressed in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis. METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with low potential of lymphogenous metastasis Hca-P and its synogenetic cell line Hca-F with high metastatic potential was constructed by suppression subtracted hybridization (SSH) method.The screened clones of the subtracted library were sequenced and GenBank homology search was performed. RESULTS:Fifteen differentially expressed cDNA fragments of Hca-P were obtained which revealed 8 known genes,4 expressed sequence tags (ESTs) and 3 cDNAs showed no homology. CONCLUSION: Tumor metastasis is an incident involving multiple genes.SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.
Suppression subtracted hybridization, Liver neoplasm, Metastasis suppression genes
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唐建武, Bo Song, Jian-Wu Tang, Bo Wang, Xiao-Nan Cui, Li Hou, Lu Sun, Li-Min Mao, Chun-Hui Zhou, Yue Du, Li-Hui Wang, Hua-Xin Wang, Ren-Shu Zheng, Lei Sun
World J Gastroenterol 2005; 11, (10): 1463-1472,-0001,():
-1年11月30日
AIM: In order to obtain lymphogenous metastasisassociated genes,we compared the transcriptional profiles of mouse hepatocarcinoma cell lines Hca-F with highly lymphatic metastasis potential and Hca-P with low lymphatic metastasis potential.METHODS:Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA.In vitro transcription double-stranded cDNA was labeled with biotin (i.e.biotin-labeled cRNA,used as the probe).The cRNA probes hybridized with Affymetrix GeneChip MOE430A (containing 22 690 transcripts,including 14 500 known mouse genes and 4 371 ESTs) respectively and the signals were scanned by the GeneArray Scanner.The results were then analyzed by bioinformatics. RESULTS: Out of the 14 500 known genes investigated,110 (0.8%) were up regulated at least 23 fold.Among the total 4 371 ESTs,17 ESTs (0.4%) (data were not presented) were up regulated at least 23 fold. According to the Gene Ontology and TreeView analysis,the 110 genes were further classified into two groups:differential biological process profile and molecular function profile. CONCLUSION: Using high-throughput gene chip method,a large number of genes and their cellular functions about angiogenesis, cell adhesion,signal transduction, cell motility, transport, microtubule-based process,cytoskeleton organization and biogenesis, cell cycle, transcription, chaperone activity, motor activity, protein kinase activity, receptor binding and protein binding might be involved in the process of lymphatic metastasis and deserve to be used as potential candidates for further investigation. Cyclin D1, Fosl1, Hsp47, EGFR and AR, and Cav-1 are selected as the possible candidate genes of the metastatic phenotype, which need to be validated in later experiments. ESTs (data were not presented) might indicate novel genes associated with lymphatic metastasis. Validating the function of these genes is helpful to identify the key or candidate gene/pathway responsible for lymphatic metastasis, which might be used as the diagnostic markers and the therapeutic targets for lymphatic metastasis.
Hepatocarcinoma, Lymphatic metastasis, Cell lines Hca-F and Hca-P, Gene chip
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