李庆伟
一直从事鸟类细胞和分子进化的研究。
个性化签名
- 姓名:李庆伟
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
细胞生物学
- 研究兴趣:一直从事鸟类细胞和分子进化的研究。
李庆伟,男,博士生导师,辽宁师范大学副校长,一级特聘教授,1996年毕业于南京师范大学生命科学学院细胞遗传专业,获博士学位。现任辽宁省生物技术协会理事、辽宁省鸟类学会常务理事、辽宁省动物学会理事、辽宁省生态学会理事、辽宁省野生动物保护协会常务理事、中国遗传学会动物专业委员会委员、《遗传学报》、《遗传》杂志编委。1993年以来享受国务院政府特殊津贴,1996年首批入选国家人事部等七部委实施的“百千万人才工程”全省32人之一,1997获大连市劳动模范称号,1998年评为辽宁省青年专业技术拔尖人才,1999年获辽宁省优秀共产党员称号.1998年被聘为大连理工大学博士研究生导师,2004年被聘为大连医科大学博士生导师。
1980年中期以来一直从事鸟类细胞和分子进化的研究,先后完成了280余种鸟类染色体核型和部分种类的G、C带型的研究。首次提出鸟类染色体进化的“瀑布模型”,是国内外同类研究鸟类染色体核型和发表论文最多的学术团队之一,出版了国际上第一部《Chromosome Atlas of Birds》专著。90年代初又率先开展了鸟类mtDNA分子进化的研究,首次发现鸟类增大的mtDNA(18853bp),并首次将鸟类线粒体基因的tRNA二级结构引入鸟类种群特征的比较研究中,取得了很好的结果,在鸟类分子进化研究领域做出了原创性工作,得到了国内外同行的高度评价。李庆伟教授具有很强的学术前瞻性,2000年又率先在国际上开展了日本七鳃鳗基因组学和蛋白质组学的研究,并对其重要功能基因和蛋白进行了生物制药的研发研究,取得了具有创新性的研究成果。1994年以来先后主持过5项国家自然科学基金项目,1项海洋“863”项目,省市级项目10余项,发表论文80余篇,其中SCI收录10多篇。主编、参编学术专著4部,近年获辽宁省政府科学二等奖1项,三等奖2项,申请专利3项。同时在李庆伟教授的倡导下,2004年10月在辽宁师范大学成立了海洋生物功能基因与蛋白质组学研究所,组建了一个优秀的学术创新团队,积极地开展日本七鳃鳗功能基因学、蛋白质组学与海洋生物制药开发的研究。
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成果阅读
402
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成果数
7
李庆伟, SUN Yi, MA Fei, XIAO Bing, ZHENG Junjie, YUAN Xiaodong, TANG Minqian, WANG Li, YU Yefei & Li Qingwei
Science in China Ser. C Life Sciences 47 6(2004)510-520,-0001,():
-1年11月30日
The complete mitochondrial genomes of Asio flammeus and Asio otus were sequenced and found to span 18858 bp and 18493 bp,respectively.It is surprising to find the former to be the largest among all avian mitochondrial genomes sequenced so far.The two genomes have very similar gene order with that of Gallus gallus,neither contains the pseudo control region,but both have a single extra base,namely Cytidine,at position 174 in ND3 gene.The control regions of Asio flammeus and Asio otus’ mitochondrial genomes span 3288 bp and 2926 bp respectively,which are the longest among vertebrates except for Myxine glutinosa and contribute to the large size of two genomes.The 3′end of the control region of Asio flammeus and Asio otus contains many tandemly repeated sequences,which are highly similar to a putative control element,i.e.Mt5,and may form stable stem-loop secondary structures.Such repeated sequences probably play an important role in regulating transcription and replication of mitochondrial genome.Our results may provide important clues for uncovering the origin and evolution mechanisms of mitochondrion genome.
Asio flammeus, Asio otus, mitochondrial genome, control region, repeated sequence.,
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【期刊论文】Phylogeny of the bears (Ursidae) based on nuclear and mitochondrial genes
李庆伟, Li Yu a, b, c, Qing-wei Li d, O.A. Ryder e, Ya-ping Zhang a, *
Molecular Phylogenetics and Evolution 32(2004)480-494,-0001,():
-1年11月30日
The taxomic classification and phylogenetic relationships within the bear family remain argumentative subjects in recent years.Prior investigation has been concentrated on the application of different mitochondrial(mt)sequence data,herein we employ two nuclear single-copy gene segments,the partial exon 1 from gene encoding interphotoreceptor retinoid binding protein(IRBP)and the complete intron 1 from transthyretin(TTR)gene,in conjunction with previously published mt data,to clarify these enigmatic problems.The combined analyses of nuclear IRBP and TTR datasets not only corroborated prior hypotheses,positioning the spectacled bear most basally and grouping the brown and polar bear together but also provided new insights into the bear phylogeny,suggesting the sister-taxa association of sloth bear and sun bear with strong support.Analyses based on combination of nuclear and mt genes differed from nuclear analysis in recognizing the sloth bears as the earliest diverging species among the subfamily ursine representatives while the exact placement of the sun bear did not resolved.Asiatic and American black bears clustered as sister group in all analyses with moderate levels of bootstrap support and high posterior probabilities.Comparisons between the nuclear and mtDNA findings suggested that our combined nuclear dataset have the resolving power comparable to mtDNA dataset for the phylogenetic interpretation of the bear family.As can be seen from present study,the unanimous phylogeny for this recently derived family was still not produced and additional independent genetic markers were in need.
Interphotoreceptor retinoid binding protein, Transthyretin, Evolution, Phylogenetic analysis
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李庆伟, Li Yu a, b, c, Qing-wei Li d, O.A. Ryder e, Ya-ping Zhang a, b, *
Molecular Phylogenetics and Evolution 33(2004)694-705,-0001,():
-1年11月30日
Phylogenetic relationships among 37 living species of order Carnivora spanning a relatively broad range of divergence times and taxonomic levels were examined using nuclear sequence data from exon 1 of the IRBP gene(1.3kb)and first intron of the TTR gene(1kb).These data were used to analyze carnivoran phylogeny at the family and generic level as well as the interspecific relationships within recently derived Felidae.Phylogenetic results using a combined IRBP + TTR dataset strongly supported within the superfamily Califormia,the red panda as the closest lineage to procyonid-mustelid(i.e.,Musteloidea)clade followed by pinnipeds(Otariidae and Phocidae),Ursidae(including the giant panda),and Canidae.Four feliform families,namely the monophyletic Herpestidae,Hyaenidae,and Felidae,as well as the paraphyletic Viverridae were consistently recovered convincingly.The utilities of these two gene segments for the phylogenetic analyses were extensively explored and both were found to be fairly informative for higher-group associations within the order Carnivora,but not for those of low level divergence at the species level.Therefore,there is a need to find additional genetic markers with more rapid mutation rates that would bediagnostic at deciphering relatively recent relationships within the Carnivora.
Interphotoreceptor retinoid binding protein, Transthyretin, Molecular evolution, Phylogenetic analysis
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33浏览
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李庆伟, 孙毅①, 马飞①, 肖冰①, 郑俊杰②, 袁晓东②, 汤敏谦②, 王黎③, 于业飞③, 李庆伟①**
中国科学C辑生命科学,2004,34(6):527~536,-0001,():
-1年11月30日
利用Long-PCR和PrimerWalking结合克隆测序法对短耳鸮和长耳鸮线粒体基因组进行了全序列测定,结果表明:短耳鸮mtDNA序列全长为18858bp,长耳鸮mtDNA全长为18493bp,其中短耳鸮mtDNA是目前已知最长的鸟类线粒体基因组,两种鸮类的基因组结构和基因排列顺序与家鸡相同,无假控制区,在ND3基因174位点都存在一个额外插入的胞苷酸(C).控制区序列异常增大是造成这两种鸟类mtDNA增大的主要原因,短耳鸮控制区长度为3288bp,长耳鸮为2926bp,这是目前已知的脊椎动物线粒体基因组中仅次于盲鳗的最大的控制区,在其控制区3,端存在大量的串联重复序列,分析发现这两种鸮类的重复序列和Mt5调控元件有较高的序列相似性,且能形成多重的茎环二级结构,这表明该重复序列可能具有一定的生理功能,影响线粒体基因组的复制或转录表达,从而使相应物种具有更大的选择优势,以适应环境和生存竞争.
短耳鸮, 长耳鸮, 线粒体基因组控制区, 重复序列
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【期刊论文】猛禽类15种鸟类线粒体tRNA基因序列及二级结构的比较研究
李庆伟, 王翔, 孙毅, 袁晓东, 汤敏谦, 王黎, 于业飞, ①
遗传学报,2004,31(4):411~419,-0001,():
-1年11月30日
利用PCR方法扩增13个猛禽类物种线粒体基因组中3个主要的tRNA基因簇:IQM(tRNAIle-tRNAGln-tRNAMet)、WANCY(tRNATrp-tRNAAla-tRNAAsn-tRNACys-tRNATyr)和HSL(tRNAHis-tRNASer(AGY)-tRNALeu(CUN)),测序后结合GenBank已登陆的游隼和普通相应序列探讨猛禽类共15种鸟类的分子系统进化。3个目的片段长度分别为212~214bp、353~362bp、205~208bp,通过比较这些tRNA基因序列和二级结构差异,发现可变核苷酸位点占47%,这些变异中67%出现在环区,且存在插入和(或)缺失。茎区相对保守,其中一些变异如双链的互补性碱基突变、G-U配对等对于维系tRNA二级结构的稳定性非常重要。以夜鹰为外群分别构建了15个猛禽类物种共11个线粒体tRNA基因全序列和茎区序列的NJ树和MP树,其中基于全序列的系统发育树分支具有较高的自引导值,因此该数据集所反映的猛禽类系统发育关系可能更接近真实水平。系统发育分析显示,隼形目鹰科更接近于鸱科而不是隼科,而草科的分类地位也与传统的形态学和DNA-DNA杂交数据的结论存在分歧。比较物种间tRNA基因二级结构发现,部分tRNA基因中的核苷酸插入和缺失特征在科水平具有共同衍征,提示这些特征对于猛禽类科间系统发育关系具有较高的参考价值。
猛禽类, tRNA 基因簇, 二级结构, 系统发育
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【期刊论文】光间受体视黄类物质结合蛋白(IRBP)的研究概况
李庆伟, 于黎, ), 李庆伟), 张亚平)
生物化学与生物物理进展,2002,29(1):138,-0001,():
-1年11月30日
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