张成孝
主要从事电分析化学、电化学发光分析和电化学的研究。
个性化签名
- 姓名:张成孝
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:-
-
学科领域:
分析化学
- 研究兴趣:主要从事电分析化学、电化学发光分析和电化学的研究。
张成孝,男,1958年1月生于陕西省耀县。日本东京工业大学博士,现任陕西师范大学化学与材料科学学院教授,博士生导师,院长,生物电分析化学研究室主任。主要从事电分析化学、电化学发光分析和电化学的研究。1986年至1995年,主要从事配合物极谱催化波的研究,主持完成的 “金属-杂环类试剂配合物极谱催化波的研究”,1992年获陕西省教委科技进步奖,1992年获中国化学会优秀青年化学奖,1993年获国家科技成果完成者证书。1995年受日本学术振兴会资助赴日本东京工业大学生命理工学院进行合作研究和学习,主要从事生物传感器和均相电化学发光免疫分析法的研究,合成和筛选了40多种配合物电子介体,研究了这些介体的电催化性能;完成了基于毛细作用进样的一次性生物传感器的研究。近几年提出了电化学溶出发光分析法、电生不稳定试剂化学发光法、能量转移电化学发光法、均相电化学发光免疫法。2002年3月至2003年3月在美国杰克狲州立大学从事生物电化学博士后研究。2006年2月至2006年5月美国杰佛罗里达国际大学从事化学与生物化学系博士后研究员。目前主要进行纳米电化学生物传感器和纳米电化学发光生物传感器的研究,现主持国家自然科学基金项目和陕西省政府项目4项。先后在国内外发表研究论文90余篇,其中SCI源期刊论文20余篇,出版教材5部,获得陕西省科技进步二等奖1项和陕西省优秀教学成果奖2项。2006年获政府特殊津贴。
-
主页访问
1481
-
关注数
0
-
成果阅读
483
-
成果数
9
【期刊论文】Homogeneous electrogenerated chemiluminescence immunoassay for the determination of digoxin
张成孝, Honglan Qi, Chengxiao Zhang ∗
Analytica Chimica Acta 501(2004)31-35,-0001,():
-1年11月30日
A novel homogeneous electrogenerated chemiluminescence immunoassay (ECLIA) for the determination of small hapten was developed. As a model system, digoxin was investigated while luminol served as luminescence label and BSA served as carrier protein. Digoxin was indirectly heavily labeled with luminol throughBSAto form luminol-BSA-digoxin conjugate. The immunocomplex of luminol-BSA-digoxin conjugate with anti-digoxin antibody underwent less ECL reaction than luminol-BSA-digoxin conjugate after immunoreaction took place. Two immunoassay formats, directly homogeneous immunodetection for anti-digoxin antibody and competitive immunoassay for digoxin, were proposed to determine anti-digoxin antibody and digoxin, respectively. The anti-digoxin antibody concentration was determined in the range from 1/50,000 to 1/2000 dilution. The ECL intensity versus digoxin concentration was linear in the range from 5.0×10−10 to 3.0×0−8 gml−1. The detection limit was 2.8×10−10 gml−1. The relative standard derivation for 1.0×10−9 gml−1 was 5.1%. The proposed method has been applied to assay digoxin in human control serum with satisfactory results.
Electrogenerated chemiluminescence immunoassay, Digoxin, Luminol, Heavily labeled hapten
-
64浏览
-
0点赞
-
0收藏
-
0分享
-
159下载
-
0评论
-
引用
张成孝, Chengxiao ZHANG † and Honglan QI
ANALYTICAL SCIENCES JULY 2002, VOL. 18 819 2002,-0001,():
-1年11月30日
-
35浏览
-
0点赞
-
0收藏
-
0分享
-
200下载
-
0评论
-
引用
张成孝, Chengxiao Zhang a, Tetsuya Haruyama b, Eiry Kobatake b, Masuo Aizawa b, ∗
Analytica Chimica Acta 442(2001)257-265,-0001,():
-1年11月30日
A novel disposable electrochemical capillary-fill device for glucose was fabricated using thick-film technology and incorporating a water-soluble layer bearing Os-complex mediator and glucose oxidase. A simple dual-electrode system used as a transducer consisted of three carbon stripes for a working electrode and an Ag/AgCl strip for a reference/counter electrode. The reagent ink, containing glucose oxidase, osmium-Tris(4,7-dimethyl-1,10-phenanthroline), and water-soluble binder, was drop-coated on the biosensor base material between the strips of the carbon electrode. The electrode part was covered with thin glass cover in order to construct a microliter electrochemical capillary-fill. A drop of test solution is automatically introduced to the microchannel by capillary action and then a water-soluble layer bearing glucose oxidase, the mediator and buffer salts becomes solubilized and quickly dispersed throughout the volume of the microchannel. The composition of the enzyme and mediator in the water-soluble layer was optimized. The constructed device showed a wide linear response range for glucose from 0.1 to 25 mM, fast response, and good storage stability. The present investigation showed that an efficient biosensor was constructed with an extremely low loading of enzyme and mediator.
Biosensor, Disposable electrode, Glucose, Capillary, Osmium complex
-
75浏览
-
0点赞
-
0收藏
-
0分享
-
167下载
-
0评论
-
引用
张成孝, Chengxiao Zhang a, Qiang Gao a, Masuo Aizawa b, *
Analytica Chimica Acta 426(2001)33-41,-0001,():
-1年11月30日
A novel flow injection analytical system with a screen-printed enzyme sensor incorporating Os-complex mediator has been developed. A newly created glucose biosensor was fabricated using thick-film technique and co-immobilization of glucose oxidase and Os-complex on a permeable membrane. The composition of the enzyme and mediator in the membrane and the analytical conditions were optimized. The flow injection electrochemical system showed that glucose was detected linearly in the concentration range from 0.1 to 10 mMwith a detection limit of 0.03 mMby 50ml of sample injection, giving a throughout of about 40 samples per hour. The glucose biosensors retained their constant response after more than 100 injections and storage over a month. The designed electrochemical flow system with a disposable biosensor is suitable for automatic and rapid determination of glucose.
Glucose biosensor, Printed electrode, Glucose, Glucose oxidase, Flow injection analysis
-
80浏览
-
0点赞
-
0收藏
-
0分享
-
71下载
-
0评论
-
引用
张成孝, Chengxiao Zhang a, T. Haruyama b, E. Kobatake b, M. Aizawab
Analytica Chimica Acta 408(2000)225-232,-0001,():
-1年11月30日
A series of novel substituted-1, 10-phenanthroline complexes of osmium and ruthenium have been synthesized and characterized electrochemically with respect to their ability to act as electron transfer mediator for glucose oxidase. These complexes exhibit high second-order rate constants for reduced glucose oxidase in the order of 106-107M−1 s-1. The complexes bearing electron-donating substituents give a significantly higher rate constant with glucose oxidase and lower redox potential than those bearing electron-accepting substituents. 4, 7-Dimethy-1, 10-phenanthroline and 3, 4, 7, 8-tetramethy-1, 10-phenanthroline complexes of osmium have been characterized in details as mediator for glucose oxidase. The application of the family of mediators in amperometric biosensors seems feasible.f
Mediator, Osmium complex, Biosensor, Glucose oxidase, Glucose
-
54浏览
-
0点赞
-
0收藏
-
0分享
-
251下载
-
0评论
-
引用
【期刊论文】Highly sensitive electrochemical luminescence determination of thiamine
张成孝, Chengxiao Zhang a, *, Guojun Zhou a, Zhujun Zhang a, M. Aizawa b
Analytica Chimica Acta 394(1999)165-170,-0001,():
-1年11月30日
A highly sensitive electrochemical luminescence (ECL) method has been developed for the determination of thiamine based on electrochemically oxidizing tbiamine with rhodamine B as a sensitizer. A weak ECL signal of thiamine was electrochemically generated on a platinum electrode in an alkaline solution and strongly enhanced in the presence of rhodamine B. Under the condition of 0.10mM rhodamine B, 6mM CTMAB and 0.36M sodium hydroxide, the response to the concentration of tbiamine is linear range from 0.1 μgml 1 to 2μgml 1 and a detection limit of 0.08pg ml 1 can be achieved. This method has been successfully applied to the determination of thiamine in pbarmaceutical preparations. The energy transfer ECL scheme is also postulated.
Elecnochemical luminescence, Thiamine, Rhodamine B, Energy transfer
-
44浏览
-
0点赞
-
0收藏
-
0分享
-
147下载
-
0评论
-
引用
【期刊论文】Flow injection chemiluminescence determination of isoniazid with electrogenerated hypochlorite
张成孝, Jiachu Huang, Chengxiao Zhang, Zhujun Zhang
Fresenius J Anal Chem (1999) 363: 126-128,-0001,():
-1年11月30日
A flow-injection chemiluminescence method for the determination of isoniazid based on the sensitizing effect of isoniazid on the chemiluminescence generating luminol-hypo-chlorite reaction is described. The hypochlorite was electrogen-erated on-line by constant current electrolysis, thus, eliminating instability of hypochlorite solution prepared from commer-cially available sodium hypochlorite. The calibration graph is linear in the range 1x10-8 to 1x10-6gmL-1, and the detection limit is 6x10-9gmL-1. The relative standard deviation for de-termination of 5x10-8gmL-1 is 2.8%. The proposed method has been successfully applied to the determination of isoniazid in pharmaceutical preparations.
-
46浏览
-
0点赞
-
0收藏
-
0分享
-
43下载
-
0评论
-
引用
张成孝, Chengxiao Zhanga, Jiachu Huang, a, Zhujun Zhanga, *, Masuo Aizawab
Analytica Chimica Acta 374(1998)105-110,-0001,():
-1年11月30日
A flow injection method for the determination of catecholamines based on the inhibition of the intensity of chemiluminescence from the luminol-hypochlorite system is described. The hypochlorite was electrogenerated on-line by constant cun-ent electrolysis, resulting in the elimination of the instability of hypochlorite solution prepared from commercially available sodium hypochlorite. The detection limits are 6
Flow injection, Chemilrminescence, Catecholamine, Luminol, Elecnolysis, Pharmaceutical analysis
-
46浏览
-
0点赞
-
0收藏
-
0分享
-
95下载
-
0评论
-
引用
张成孝, Chengxiao Zhang, Sichun Zhang and Zhujun Zhang*
Analyst, June 1998, Vol. 123 (1383-1386),-0001,():
-1年11月30日
A novel stripping chemiluminescence (CL) method in situ has been developed which is a combination of selective stripping and CL detection in situ. This method consists of a concentration (pre-electrolysis) step, in which the analyte metal ion is reduced at a controlled potential whilst being deposited as the metal on a solid electrode placed in an electrogenerated chemiluminescence flow cell, a subsequent media exchange step and finally the stripping-CL step, in which the CL emission on the electrode surface is detected in situ when the CL reagent solution is delivered into the cell to react with the metal ion which has just been stripped by oxidization. The concentration of analyte is quantified by CL intensity. Copper ion as a representative analyte was investigated using constant potential stripping and CL detection of a luminol-copper (ii)-cyanide system. The response to the concentration of copper is linear in the range 0.040-40mg dm23 and an extremely low detection limit of 0.02 mg dm23 can be achieved in a deposition time of 2min. The method developed has been applied successfully to the determination of copper in water samples. The method shows excellent selectivity and sensitivity compared with the associated CL and square wave anodic stripping voltammetry method.
Stripping analysis, chemiluminescence, flow system, copper, luminol,, water
-
39浏览
-
0点赞
-
0收藏
-
0分享
-
38下载
-
0评论
-
引用