李春杰
禾草内生真菌、牧草与草坪草病理学、种子病理学及农业微生物学
个性化签名
- 姓名:李春杰
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
草原学
- 研究兴趣:禾草内生真菌、牧草与草坪草病理学、种子病理学及农业微生物学
李春杰,男,汉族,生于1968年3月,中共党员。博士,教授,博士生导师。现任草地农业教育部工程技术研究中心副主任。
1993年至今,从事禾草内生真菌、牧草与草坪草病理学、种子病理学及农业微生物学方面的科研和教学工作。其中:2000年~2001年,在荷兰百绿研究所(Barenbrug Research Holland)从事牧草与草坪草病害研究工作。2002年~2003年,在德国应用科技大学生物技术与品质保证实验室从事禾草内生真菌的研究工作。2006年~2007年,美国肯塔基大学(University of Kentucky)植物病理学系博士后工作。2008年~今:中国植物病理学会种子病理学专业委员会副主任委员、青年工作委员会副主任委员;2003年~今:中国草学会草地植保专业委员会理事、副秘书长;2002年~今:甘肃省植保学会 理事、副秘书长。
2009年,作为第八完成人,承担的“草业科学学科设计与人才培养体系建设”获国家教学成果特等奖。1999年,作为第三完成人,承担的“牧草病害及其防治”项目获国家科技进步三等奖。1997年,作为第五完成人,承担的“牧草种子质量与检测技术”项目获农业部科技进步三等奖。2008年,入选教育部“新世纪优秀人才支持计划”。2007年,被评为第八届“甘肃优秀青年”。2001年,入选甘肃省跨世纪学术带头人“333科技人才工程”第一、二层次。2000年,获甘肃省第四届青年科技奖。
主讲本科生《农业微生物学》和《草地保护学》;硕士研究生的《真菌学》、《高级牧草病理学》、《草坪草病害研究方法》和《有害生物综合治理》课程。
指导博硕士研究生18名,其中已获得硕士学位7名。
在研项目:国家自然科学基金项目“醉马草内生真菌多样性及其对家畜的影响”(30771531,2008~2010年)。国家973计划子课题”禾草内生真菌共生体的研究”(2007CB108902-4, 2007-2012年)。国家科技支撑子课题“利用内生真菌进行禾草育种的研究” (2008BABD3B06,2008~2011)。教育部新世纪优秀人才支持计划“醉马草内生真菌共生体的研究”(2009~2011)。农业部草原监理中心委托项目“西北地区草地植被长势监测研究”(2009~2010)。兰州市南北两山绿化指挥部委托项目“兰州市高削坡绿化草灌品种筛选与抗旱性评价”(2009~2010)和“绿桥工程-草地恢复技术与模式”(2009~2010)。
获得国家发明专利一项。正式发表研究论文70余篇,其中第一作者29篇,通讯作者14篇。副主编专著2部,参编著作和教材3部。其中在Plant Disease, Mycological Research, Mycotaxon, Microbiological Research, Conservation Genetics, Mycologia等SCI期刊上已发表论文11篇,国际会议上发表论文7篇,国际会议上交流展板9幅。
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20
李春杰, Xingxu Zhang, Xiaomei Fan, Chunjie Li, Zhibiao Nan
,-0001,():
-1年11月30日
The effects of cadmium (Cd) on germination,and antioxidative enzyme activity (AEA) involving superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase, and on amounts of malondialdehyde and proline present within Achnatherum inebrians, were determined for specimens infected (E?) vs. non-infected (E-) by Neotyphodium gansuense, and cultivated in the presence of various concentrations of CdCl2 (0, 50, 100, 200 and 300 lmol/l). Under high Cd concentrations (100, 200 and 300 lM), E? (vs. E-) specimens exhibited a higher germination rate and index, and higher values for shoot length, root length and dry biomass, but there was no significant difference (P[0.05) under low Cd concentrations (0 and 50 lM). AEA and the proline content increased, but malondialdehyde content declined in the E? (vs. E-) specimens under high Cd concentrations (100, 200 and 300 lM). There was no significant difference (P[0.05) under low Cd concentrations (0 and 50 lM). Endophyte infection was concluded to be of benefit to the germination and anti-oxidative mechanisms within A. inebrians under plant exposures to high CdCl2 concentrations.
Cadmium, Achnatherum inebrians, Germination, Antioxidative enzyme, Endophyte
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李春杰, C.J. Li, Z.F.Wang, N.Chen, and Z.B.Nan
APS Journals 93(2009)673-674,-0001,():
-1年11月30日
Phytopathology, Plant Disease, MPMI
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李春杰, Chunjie Li, Zhibiao Nan*, Fei Li
Microbiological Research 163(2008)431-440,-0001,():
-1年11月30日
Biological and physiological characteristics of Neotyphodium gansuense were compared with Neotyphodium coenophialum and Epichloe festucae at a range of temperatures and pH values, and on carbon and nitrogen amended media. N. gansuense was able to grow at 10-30 1C, but not at 51C, and slowly at 351C. The optimal temperature for both N. gansuense and N. coenophialum was 251C, but that of E. festucae was 20-25 1C. The optimal pH ranges for mycelial growth of N. gansuense, N. coenophialum and E. festucae were 5-9, 5-9 and 5-7, respectively. The Neotyphodium and Epichloe
Neotyphodium gansuense, Endophyte, Achnatherum inebrians, Biology, Physiology
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【期刊论文】Interactions of Neotyphodium gansuense, Achnatheruminebrians, and plant-pathogenic fungi
李春杰, Chun-jie LI, Jia-hui GAO, Zhi-biao NAN*
mycologicalresearchIII (2007) 1220-1227,-0001,():
-1年11月30日
Interactions of Neotyphodium gansuense, Achnatherum inebrians, and nine fungal pathogens were studied by tests of inhibition of four fungal pathogens by Neotyphodium endophytes in vitro and by inoculation of nine fungal pathogens on detached leaves of endophyteinfected (Et) and endophyte-free (E_) plants. Compared with the controls, most isolates of N. gansuense significantly inhibited the growth in vitro of, in decreasing order of inhibition, Bipolaris sorokiniana, Curvularia lunata, Fusarium acuminatum, and Alternaria alternata. Inhibition zones appeared between pathogens and some isolates of N. gansuense. Some isolates of N. gansuense significantly inhibited sporulation of B. sorokiniana, A. alternata, and C. lunata. However, there was no significant inhibition of F. acuminatum and a few isolates significantly increased sporulation. The leaf inoculation trial indicated that almost all fungal pathogens were able to cause lesions on detached leaves regardless of endophyte status. Both the number and size of disease lesions on Et A. inebrians leaves caused by A. alternate.
Detached leaves inoculation, Drunken horse grass, Dual culture, Fungal endophytes, Fungal pathogens
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【期刊论文】A new Neotyphodium species symbiotic with drunken horse grass (Achnatherum inebrians) in China
李春杰, CHUNJIE LI, ZHIBIAO NAN*, VOLKER H. PAUL, PETER D. DAPPRICH, YONG LIU
MYCOTAXONVolume 90 1(2004)141-147,-0001,():
-1年11月30日
A new endophytic fungus has been isolated from Achnatherum inebrians collected from Gansu Province, China. The morphological and cultural characters are used to describe the new species, Neotyphodium gansuense.
taxonomy, new taxon, alkaloids
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【期刊论文】Twelve polymorphic microsatellite loci for Achnatherum inebrians(Poaceae)
李春杰, Na Chen, Yan-Zhuo Yang, Xiao-li Yang, Xing-xu Zhang, Chun-jie Li
Conserv Genet 9(2008)961-963,-0001,():
-1年11月30日
Microsatellites are often highly variable and abundant in most complex genomes, therefore are widely used in population genetic studies. In this study, twelve polymorphic microsatellite loci were isolated and characterized for the Achnatherum inebrians, a plant abundant in grasslands of Northwest China. Characterization of 24 A. inebrians individuals form four geographically distant populations (Gansu, Qinghai, Xinjiang and Inner Mongolia provinces) showed moderate to high allelic diversity ranging from 3 to 13 alleles per locus, and the expected heterozygosity ranging from 0.41 to 0.67. No evidence of linkage disequilibrium was found for any locus pairwise comparisons. The markers described here will be useful for investigating the genetic diversity, genetic structure and gene flow of this species.
Achnatherum inebrians, Genetic diversity, Microsatellites Conservation
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李春杰, Na Chen, Yang Yang, Chun-Jie Li, Zhi-Biao Nan
Conserv Genet 9(2008)1699-1701,-0001,():
-1年11月30日
Achnatherum sibiricum is a threaten and toxic perennial bunchgrass mainly in the north of China. We developed 11 polymorphic microsatellite loci from A. sibiricum by combining biotin capture method. After validating and scoring, these loci were polymorphic in a test population with the range of alleles from four to 13 per locus. The observed and expected heterozygosities ranged from 0.1649 to 0.5624 and from 0.3071 to 0.8826, respectively. All 11 microsatellite markers were in Hardy– Weinberg equilibrium. These polymorphic microsatellites will be useful for genetic diversity analysis and population structure construction for A. sibiricum.
Achnatherum sibiricum, Genetic diversity, Microsatellites, Conservation
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李春杰, Xingxu Zhang, Chunjie Li ∗, Zhibiao Nan
,-0001,():
-1年11月30日
The effects of cadmium on biomass production and growth parameters of drunken horse grass(Achnatherum inebrians) over an 8-week period were determined in a controlled-environment experiment.Changes were determined for relative water content, anti-oxidative enzymes (i.e., catalase (CAT),superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX)) and for H2O2 content,as well as levels of proline, malondialdehyde (MDA), and chlorophylls “a” and “b” present within leaves infected with Neotyphodium gansuense vs. non-infected controls. observations began 4 weeks after addition of CdCl2 (0, 50, 100 and 200 M) to the nutrient solution. Under high concentrations (100 and200 M) of CdCl2, endophyte-infected plants produced more biomass and had higher values for plantheight and tiller number compared to non-infected controls, but there was no significant difference (P > 0.05) under 0 and 50 M CdCl2. Anti-oxidative enzyme activities, H2O2 concentration, and chlorophylls“a” and “b” levels increased, but proline and malondialdehyde content declined in the infected plants vs. non-infected plants under high (100 and 200 M) concentrations of CdCl2. There was no significant difference (P>0.05) under 0 and 50 M CdCl2. Endophyte infection was concluded to be of benefit to the growth and anti-oxidative mechanisms within A. inebrians under high concentrations exposures to CdCl2.
Cadmium, Achnatherum inebrians, Growth, Anti-oxidative enzyme, Endophyte
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【期刊论文】New Neotyphodium endophyte species from the grass tribes Stipeae and Meliceae
李春杰, Christina D. Moon, Jean-Jacques Guillaumin, Catherine Ravel, Chunjie Li, Kelly D. Craven, Christopher L.Schardl
Mycologia, 99(6),2007,895-905.,-0001,():
-1年11月30日
Several species of Achnatherum (grass tribe Stipeae) and Melica (tribe Meliceae) typically are infected by nonpathogenic, seed-transmissible fungi with characteristics of Neotyphodium species (anamorphic Clavicipitaceae). Molecular phylogenetic studies clearly have distinguished the endophytes from Achnatherum inebrians (from Xinjiang Province, China), A. robustum and A. eminens (both from North America) and indicate that the A. inebrians endophyte comprises a unique nonhybrid lineage within the Epichloe and Neotyphodium phylogeny, whereas the endophytes of A. robustum, and A. eminens are hybrids with multiple Epichloe species (holomorphic Clavicipitaceae) as ancestors. Likewise distinct hybrid origins are indicated for Neotyphodium species from the European Melica species, M. ciliata and M. transsilvanica, the South African species M. decumbens and M. racemosa, and the South American species M. stuckertii. Neotyphodium species have been described from A. inebrians from Gansu Province, China, (N. gansuense), A. eminens (N. chisosum), M. stuckertii (N. tembladerae) and the South African Melica species (N. melicicola). However the endophytes from A. robustum and the European Melica species have not been described and the phylogenetic relationships of N. gansuense have not been investigated. Here we report a comprehensive study of morphological features and phylogenetic analyses of b -tubulin and actin gene sequences on an expanded collection of endophytes from the Stipeae and Meliceae. These data provide a firm foundation for the description of two new Neotyphodium species, N. guerinii from M. ciliata and M. transsilvanica, and N. funkii from A. robustum. We also propose the new variety, N. gansuense var. inebrians for endophytes of A. inebrians from Xinjiang Province, which are morphologically and phylogenetically distinct from, yet clearly related to, N. gansuense from Gansu Province.
Achnatherum, Clavicipitaceae, epichloe endophytes, evolution, grasses, hybridization, Melica, Poaceae, symbiosis
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【期刊论文】Root-invading fungi of milk vetch on the Loess Plateau, China
李春杰, Yali Yin a, b, Z.B.Nan a, *, Chunjie Li a, Fujiang Hou a
Agriculture, Ecosystems and Environment 124(2008)51-59,-0001,():
-1年11月30日
Root-invading fungi of milk vetch (Astragalus adsurgens) at different growth stages grown in three ages of sown pasture soil were studiedin Huanxian county, Gansu province, China. Pathogenicity was tested by seed and soil inoculation. A total of 44 fungi were isolated from milkvetch roots, 32 from the roots of field plants and 37 from the roots of potted seedlings. The total percentage of root segments yielding fungalcolonies increased with age of plants; they were 70.5%, 82.3% and 97.8% in 1-, 3-, and 5-year-old plant roots, respectively, differences thatwere significant (P<0.05). The frequency of isolation from roots of plants at re-greening, branching, flowering and yellowing growth stageswere 62.0%, 95.3%, 93.0% and 83.7%, respectively. The total percentage of root segments yielding fungal colonies from 3-, 6-and 12-weekoldpotted seedlings were 21.8%, 44.7% and 32.0%, respectively, differences that were also significant (P<0.05). Seedlings were easilyinfected and symptoms of damping-off disease were common in seedlings younger than 3-week-old. Of the 44 fungi isolated and tested forpathogenicity by seed inoculation, 19 decreased seed germination rate, 12 decreased seedling shoot length, and 22 decreased seedling rootlength. Fusarium solani, F. oxysporum, F. chlamydosporum, F. avenaceum and Gliocladium roseum were strongly pathogenic to milk vetch. Insoil inoculation tests, these same fungi (except for F. chlamydosporum) decreased seedling root dry matter production significantly(P<0.05), but seed germination and seedling survival were not affected by any of these fungi.
Milk vetch, Pasture, Root-invading fungi, Pathogenicity, Fusarium species, China
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